Mutagenetix > Incidental Mutation

Incidental Mutation 'R9688:Naa16'

729008

Institutional Source

Beutler Lab

Gene Symbol

Naa16

Ensembl Gene

ENSMUSG00000022020

Gene Name

N(alpha)-acetyltransferase 16, NatA auxiliary subunit

Synonyms

Narg1l, 1300019C06Rik

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R9688 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

79325269-79390778 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 79335869 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 820 (Y820*)

Ref Sequence

ENSEMBL: ENSMUSP00000022597 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022597]

AlphaFold

Q9DBB4

Predicted Effect

probably null
Transcript: ENSMUST00000022597
AA Change: Y820*

SMART Domains

Protein: ENSMUSP00000022597
Gene: ENSMUSG00000022020
AA Change: Y820*

Domain	Start	End	E-Value	Type
TPR	46	79	2.99e1	SMART
TPR	80	113	2.98e-3	SMART
Blast:TPR	224	257	1e-10	BLAST
TPR	374	407	9.96e0	SMART
TPR	408	441	7.47e0	SMART
low complexity region	616	633	N/A	INTRINSIC
Blast:TPR	672	705	3e-12	BLAST
low complexity region	830	841	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700093K21Rik	A	G	11: 23,519,067		probably null	Het
Abca2	G	C	2: 25,434,447	A272P	possibly damaging	Het
Adarb1	T	A	10: 77,311,265	D479V	probably damaging	Het
Amz2	T	C	11: 109,429,939	F162S	probably damaging	Het
Ano4	A	T	10: 89,344,644	M1K	probably null	Het
Antxr1	G	A	6: 87,137,352	R478C	probably damaging	Het
Apobr	T	C	7: 126,587,491	S725P	probably benign	Het
Arap2	A	T	5: 62,714,766	D506E	probably damaging	Het
Ash1l	T	G	3: 88,984,717	I1301S	probably damaging	Het
Camk1g	T	A	1: 193,348,721	R305W	probably damaging	Het
Ces2g	A	T	8: 104,964,672	I147F	probably benign	Het
Cfap61	T	C	2: 146,036,838	F505L	probably damaging	Het
Cndp1	G	C	18: 84,637,857	N62K	probably benign	Het
Cog6	G	T	3: 53,009,107	Q214K	probably benign	Het
Depdc5	T	A	5: 32,897,932	Y226*	probably null	Het
Elp5	T	A	11: 69,970,599	I185F	probably damaging	Het
Ercc6	A	C	14: 32,575,798	H1334P	probably benign	Het
Ern1	T	C	11: 106,459,010	T27A	possibly damaging	Het
Esf1	A	T	2: 140,168,175	D80E	probably damaging	Het
Evpl	T	C	11: 116,234,160	K159E	probably damaging	Het
Gbx1	T	C	5: 24,526,512	Y102C	probably damaging	Het
Gjd2	A	G	2: 114,011,628	S123P	probably benign	Het
H2-M11	A	G	17: 36,548,162	Y198C	probably damaging	Het
Hps1	A	C	19: 42,766,708	V265G	probably benign	Het
Ifna16	C	A	4: 88,676,637	E74*	probably null	Het
Inca1	C	T	11: 70,688,984		probably null	Het
Jag2	A	G	12: 112,908,944	V1230A	probably benign	Het
Jam3	G	A	9: 27,098,908	T260M	probably benign	Het
Klhl5	A	T	5: 65,164,587	D652V	probably damaging	Het
L3mbtl1	A	T	2: 162,948,777	I81L	possibly damaging	Het
Lgalsl	C	T	11: 20,829,442	A68T	probably benign	Het
Map3k14	T	A	11: 103,239,233	D286V	possibly damaging	Het
Mdn1	A	G	4: 32,745,590	I4083V	probably damaging	Het
Ms4a5	T	C	19: 11,276,694	I136M	possibly damaging	Het
Nbea	G	T	3: 55,649,744	N2537K	probably benign	Het
Ncapd3	A	G	9: 27,056,053	S562G	probably benign	Het
Olfr1093	A	C	2: 86,786,661	K310N	probably damaging	Het
Olfr786	C	A	10: 129,437,098	C95*	probably null	Het
Papd7	A	G	13: 69,507,080	L425P	probably damaging	Het
Pcdhac2	A	G	18: 37,144,575	S203G	probably benign	Het
Pds5a	A	T	5: 65,654,853	F309I	probably benign	Het
Pdzd8	G	A	19: 59,345,251	L113F	probably benign	Het
Pex12	T	C	11: 83,298,431	Y4C	possibly damaging	Het
Ppfibp2	A	G	7: 107,719,241	D332G	probably benign	Het
Psg23	A	T	7: 18,610,622	W303R	probably damaging	Het
Pyy	T	A	11: 102,107,273	D27V	probably damaging	Het
Rc3h1	A	G	1: 160,942,664	Q302R	probably damaging	Het
Rhoh	A	G	5: 65,892,313		probably benign	Het
Samd9l	A	G	6: 3,377,087	M58T	probably damaging	Het
Scaf11	A	C	15: 96,415,927	H1324Q	probably damaging	Het
Serinc2	C	A	4: 130,264,340	A105S	probably damaging	Het
Setx	A	T	2: 29,146,316	S938C	probably damaging	Het
Sgo2a	A	T	1: 58,017,578	T974S	probably damaging	Het
Sh3rf1	T	C	8: 61,226,314	V130A	probably benign	Het
Slc43a2	T	C	11: 75,543,286	F97L	probably damaging	Het
Slc9a9	C	A	9: 95,229,054	Q642K	probably benign	Het
Soga3	T	C	10: 29,196,695	V661A	possibly damaging	Het
Sox6	G	T	7: 115,476,990	D804E	probably benign	Het
Tbx15	A	T	3: 99,326,392	R290S	possibly damaging	Het
Terf2	A	G	8: 107,094,911	S176P	probably damaging	Het
Ttc6	A	T	12: 57,673,816	H956L	possibly damaging	Het
Tti2	T	C	8: 31,154,251	V292A	probably damaging	Het
Vmn1r29	A	T	6: 58,307,754	E153V	probably benign	Het
Vmn1r55	G	T	7: 5,146,670	H251Q	probably benign	Het
Yeats4	T	C	10: 117,215,681	T224A	probably benign	Het
Yipf4	T	A	17: 74,499,083	F239Y	probably benign	Het
Zan	T	C	5: 137,468,455	D380G	probably damaging	Het
Zbtb9	T	C	17: 26,974,920	F433S	probably damaging	Het
Zic5	T	C	14: 122,464,023	K432R	unknown	Het

Other mutations in Naa16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Naa16	APN	14	79355729	missense	probably damaging	1.00
IGL01025:Naa16	APN	14	79384756	missense	probably damaging	1.00
IGL01155:Naa16	APN	14	79384715	missense	probably damaging	0.98
IGL01335:Naa16	APN	14	79345116	splice site	probably benign
IGL01981:Naa16	APN	14	79381516	missense	probably benign	0.05
IGL02230:Naa16	APN	14	79377361	splice site	probably benign
IGL02313:Naa16	APN	14	79384668	missense	probably damaging	1.00
IGL02418:Naa16	APN	14	79383366	missense	probably damaging	1.00
IGL02544:Naa16	APN	14	79335820	missense	probably damaging	1.00
IGL03051:Naa16	APN	14	79369082	missense	probably benign	0.01
IGL03064:Naa16	APN	14	79339628	missense	probably damaging	0.98
IGL03205:Naa16	APN	14	79356512	missense	possibly damaging	0.89
PIT4508001:Naa16	UTSW	14	79369087	missense	probably benign	0.15
R0651:Naa16	UTSW	14	79351392	missense	probably damaging	1.00
R1429:Naa16	UTSW	14	79359527	missense	probably benign	0.01
R1674:Naa16	UTSW	14	79387057	start codon destroyed	probably null	0.65
R1693:Naa16	UTSW	14	79351456	missense	probably damaging	1.00
R1874:Naa16	UTSW	14	79355743	missense	possibly damaging	0.62
R1992:Naa16	UTSW	14	79356491	missense	probably damaging	1.00
R2015:Naa16	UTSW	14	79345059	missense	probably damaging	1.00
R2391:Naa16	UTSW	14	79370049	missense	probably benign	0.16
R2847:Naa16	UTSW	14	79335883	missense	probably damaging	1.00
R2848:Naa16	UTSW	14	79335883	missense	probably damaging	1.00
R2877:Naa16	UTSW	14	79343298	missense	probably benign	0.00
R3884:Naa16	UTSW	14	79343262	missense	probably damaging	0.98
R4001:Naa16	UTSW	14	79343121	splice site	probably null
R4199:Naa16	UTSW	14	79355871	missense	probably damaging	1.00
R4638:Naa16	UTSW	14	79340033	splice site	probably null
R4676:Naa16	UTSW	14	79336348	unclassified	probably benign
R4690:Naa16	UTSW	14	79345057	missense	probably damaging	1.00
R4952:Naa16	UTSW	14	79345085	missense	probably damaging	1.00
R5087:Naa16	UTSW	14	79377415	missense	possibly damaging	0.68
R5104:Naa16	UTSW	14	79384700	nonsense	probably null
R5729:Naa16	UTSW	14	79355780	missense	probably damaging	1.00
R6178:Naa16	UTSW	14	79383340	missense	possibly damaging	0.93
R6960:Naa16	UTSW	14	79359471	missense	possibly damaging	0.65
R7794:Naa16	UTSW	14	79377494	missense	probably damaging	1.00
R7936:Naa16	UTSW	14	79341046	missense	possibly damaging	0.47
R8356:Naa16	UTSW	14	79359475	missense	probably benign	0.00
R8456:Naa16	UTSW	14	79359475	missense	probably benign	0.00
R8892:Naa16	UTSW	14	79390576	missense	probably benign	0.32
R8931:Naa16	UTSW	14	79344955	missense	probably damaging	1.00
R9010:Naa16	UTSW	14	79370042	missense	probably benign	0.01
R9068:Naa16	UTSW	14	79374849	missense	probably benign	0.18
R9360:Naa16	UTSW	14	79356503	missense	probably benign	0.05
X0064:Naa16	UTSW	14	79351389	missense	probably damaging	1.00
Z1177:Naa16	UTSW	14	79344979	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGCTTTGAGTCTTAGGAGCAAC -3'
(R):5'- AAATTGGAATCCTGCGTCACCC -3'

Sequencing Primer
(F):5'- GAGTCTTAGGAGCAACTTCCC -3'
(R):5'- TGGGCCTCCATAACCAGCTC -3'

Posted On

2022-10-06