Mutagenetix > Incidental Mutation

Incidental Mutation 'R1245:Vmn2r6'

152118

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r6

Ensembl Gene

ENSMUSG00000090581

Gene Name

vomeronasal 2, receptor 6

Synonyms

EG667069, EG620718

MMRRC Submission

039312-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.198) question?

Stock #

R1245 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64444916-64472855 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 64464211 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 208 (S208P)

Ref Sequence

ENSEMBL: ENSMUSP00000135148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165012] [ENSMUST00000176481]

AlphaFold

H3BK29

Predicted Effect

possibly damaging
Transcript: ENSMUST00000165012
AA Change: S119P

PolyPhen 2 Score 0.475 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000131831
Gene: ENSMUSG00000090581
AA Change: S119P

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	1.4e-72	PFAM
Pfam:Peripla_BP_6	58	244	1.2e-10	PFAM
Pfam:NCD3G	458	511	1.8e-17	PFAM
Pfam:7tm_3	542	779	3.9e-76	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000176481
AA Change: S208P

PolyPhen 2 Score 0.527 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000135148
Gene: ENSMUSG00000090581
AA Change: S208P

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	9.8e-77	PFAM
Pfam:Peripla_BP_6	142	331	3.4e-10	PFAM
Pfam:NCD3G	547	600	5.4e-17	PFAM
Pfam:7tm_3	633	867	3.9e-47	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 95.9%
20x: 91.5%

Validation Efficiency

98% (41/42)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy1	T	A	11: 7,119,410 (GRCm39)		probably benign	Het
Ahnak	A	G	19: 8,981,533 (GRCm39)	N939S	probably benign	Het
Cblb	C	A	16: 51,867,550 (GRCm39)		probably benign	Het
Cd22	A	G	7: 30,569,308 (GRCm39)	S603P	probably damaging	Het
Col6a6	G	A	9: 105,626,109 (GRCm39)	R1515C	possibly damaging	Het
Csf1r	C	A	18: 61,247,884 (GRCm39)	D317E	probably benign	Het
Dlg2	T	C	7: 92,091,803 (GRCm39)		probably benign	Het
Enpp3	G	A	10: 24,660,851 (GRCm39)		probably benign	Het
G530012D18Rik	CAGAGAGA	CAGAGAGAGA	1: 85,504,945 (GRCm39)		probably null	Het
Gm5828	A	T	1: 16,839,353 (GRCm39)		noncoding transcript	Het
Gpr137c	T	C	14: 45,516,522 (GRCm39)		probably benign	Het
Ibtk	A	G	9: 85,602,795 (GRCm39)		probably null	Het
Kctd7	A	G	5: 130,177,058 (GRCm39)	H96R	possibly damaging	Het
Lmcd1	T	A	6: 112,292,673 (GRCm39)	V175E	probably benign	Het
Lrfn2	A	T	17: 49,403,277 (GRCm39)		probably null	Het
Ltbp1	C	T	17: 75,634,189 (GRCm39)		probably benign	Het
Myc	A	G	15: 61,859,746 (GRCm39)	I140V	probably damaging	Het
Nckap1l	A	G	15: 103,364,352 (GRCm39)	E100G	probably damaging	Het
Ncs1	A	G	2: 31,174,705 (GRCm39)	N143S	probably benign	Het
Nmnat2	A	G	1: 152,987,949 (GRCm39)	D238G	probably benign	Het
Or10ag57	T	A	2: 87,218,553 (GRCm39)	V168E	probably benign	Het
Ppp2r3d	G	A	9: 101,071,593 (GRCm39)	T675I	probably damaging	Het
Psma2	A	G	13: 14,787,876 (GRCm39)	Y6C	probably damaging	Het
Rspo4	G	A	2: 151,709,846 (GRCm39)	E84K	probably damaging	Het
Shank2	G	A	7: 143,965,457 (GRCm39)	V1015I	probably benign	Het
Slc35e1	T	C	8: 73,246,415 (GRCm39)		probably benign	Het
Smg8	T	C	11: 86,974,436 (GRCm39)	D632G	possibly damaging	Het
Sppl2a	A	T	2: 126,755,441 (GRCm39)		probably benign	Het
Svep1	A	G	4: 58,066,427 (GRCm39)		probably null	Het
Ttn	T	A	2: 76,776,044 (GRCm39)	K1666M	probably damaging	Het
Ulk1	A	G	5: 110,937,206 (GRCm39)		probably null	Het
Unc80	A	G	1: 66,594,254 (GRCm39)	D1211G	possibly damaging	Het
Vmn2r112	A	G	17: 22,822,228 (GRCm39)	Q302R	probably benign	Het
Wnk1	C	T	6: 119,925,418 (GRCm39)	V1847I	probably benign	Het
Zfp653	A	T	9: 21,967,718 (GRCm39)	I529N	probably damaging	Het
Zfp948	T	C	17: 21,807,104 (GRCm39)	S99P	probably damaging	Het
Zfyve9	A	G	4: 108,550,508 (GRCm39)		probably benign	Het
Zscan29	T	C	2: 120,996,984 (GRCm39)	T246A	probably damaging	Het

Other mutations in Vmn2r6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01547:Vmn2r6	APN	3	64,445,525 (GRCm39)	missense	probably damaging	1.00
IGL01968:Vmn2r6	APN	3	64,463,766 (GRCm39)	missense	possibly damaging	0.94
IGL02009:Vmn2r6	APN	3	64,445,323 (GRCm39)	missense	possibly damaging	0.61
IGL02039:Vmn2r6	APN	3	64,463,610 (GRCm39)	missense	probably damaging	1.00
IGL02652:Vmn2r6	APN	3	64,463,749 (GRCm39)	missense	probably benign	0.24
IGL02737:Vmn2r6	APN	3	64,463,911 (GRCm39)	missense	possibly damaging	0.55
IGL02808:Vmn2r6	APN	3	64,463,917 (GRCm39)	missense	probably damaging	1.00
IGL03066:Vmn2r6	APN	3	64,472,574 (GRCm39)	missense	probably damaging	0.99
IGL03331:Vmn2r6	APN	3	64,445,428 (GRCm39)	missense	probably damaging	1.00
BB010:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
BB020:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
R0010:Vmn2r6	UTSW	3	64,466,966 (GRCm39)	nonsense	probably null
R0206:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0206:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0208:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0427:Vmn2r6	UTSW	3	64,467,008 (GRCm39)	missense	probably damaging	1.00
R0466:Vmn2r6	UTSW	3	64,463,723 (GRCm39)	missense	probably damaging	1.00
R1018:Vmn2r6	UTSW	3	64,464,261 (GRCm39)	missense	probably benign	0.00
R1104:Vmn2r6	UTSW	3	64,445,487 (GRCm39)	missense	possibly damaging	0.93
R1186:Vmn2r6	UTSW	3	64,472,488 (GRCm39)	missense	probably benign	0.01
R1295:Vmn2r6	UTSW	3	64,445,694 (GRCm39)	missense	probably damaging	1.00
R1473:Vmn2r6	UTSW	3	64,445,579 (GRCm39)	nonsense	probably null
R1498:Vmn2r6	UTSW	3	64,463,890 (GRCm39)	missense	probably damaging	1.00
R1925:Vmn2r6	UTSW	3	64,463,698 (GRCm39)	missense	possibly damaging	0.87
R2044:Vmn2r6	UTSW	3	64,445,262 (GRCm39)	missense	probably damaging	0.96
R2069:Vmn2r6	UTSW	3	64,463,519 (GRCm39)	missense	possibly damaging	0.89
R2253:Vmn2r6	UTSW	3	64,467,139 (GRCm39)	missense	probably damaging	1.00
R2261:Vmn2r6	UTSW	3	64,464,090 (GRCm39)	missense	probably benign	0.24
R2262:Vmn2r6	UTSW	3	64,464,090 (GRCm39)	missense	probably benign	0.24
R2350:Vmn2r6	UTSW	3	64,463,773 (GRCm39)	missense	probably benign	0.01
R2680:Vmn2r6	UTSW	3	64,445,707 (GRCm39)	missense	possibly damaging	0.91
R2846:Vmn2r6	UTSW	3	64,464,211 (GRCm39)	missense	possibly damaging	0.53
R2860:Vmn2r6	UTSW	3	64,454,760 (GRCm39)	missense	probably benign	0.00
R2861:Vmn2r6	UTSW	3	64,454,760 (GRCm39)	missense	probably benign	0.00
R3766:Vmn2r6	UTSW	3	64,463,929 (GRCm39)	missense	probably benign	0.19
R3870:Vmn2r6	UTSW	3	64,464,042 (GRCm39)	missense	probably damaging	0.96
R4018:Vmn2r6	UTSW	3	64,463,893 (GRCm39)	missense	probably benign	0.05
R4024:Vmn2r6	UTSW	3	64,445,671 (GRCm39)	missense	possibly damaging	0.73
R4026:Vmn2r6	UTSW	3	64,445,671 (GRCm39)	missense	possibly damaging	0.73
R4227:Vmn2r6	UTSW	3	64,445,369 (GRCm39)	missense	probably damaging	0.99
R4526:Vmn2r6	UTSW	3	64,445,145 (GRCm39)	missense	probably benign	0.32
R4570:Vmn2r6	UTSW	3	64,467,068 (GRCm39)	missense	probably benign	0.31
R4894:Vmn2r6	UTSW	3	64,454,829 (GRCm39)	missense	probably benign
R4934:Vmn2r6	UTSW	3	64,463,766 (GRCm39)	missense	probably damaging	0.99
R5057:Vmn2r6	UTSW	3	64,445,207 (GRCm39)	missense	probably damaging	1.00
R5059:Vmn2r6	UTSW	3	64,445,044 (GRCm39)	missense	possibly damaging	0.89
R5148:Vmn2r6	UTSW	3	64,464,015 (GRCm39)	missense	probably damaging	0.99
R5155:Vmn2r6	UTSW	3	64,445,935 (GRCm39)	missense	probably benign	0.44
R5179:Vmn2r6	UTSW	3	64,445,411 (GRCm39)	missense	probably benign	0.00
R5256:Vmn2r6	UTSW	3	64,464,263 (GRCm39)	missense	probably benign	0.33
R5861:Vmn2r6	UTSW	3	64,463,454 (GRCm39)	missense	probably benign	0.00
R5950:Vmn2r6	UTSW	3	64,472,652 (GRCm39)	missense	probably benign	0.05
R6081:Vmn2r6	UTSW	3	64,463,953 (GRCm39)	missense	probably benign	0.25
R6173:Vmn2r6	UTSW	3	64,467,176 (GRCm39)	missense	probably damaging	1.00
R6190:Vmn2r6	UTSW	3	64,445,424 (GRCm39)	missense	probably benign	0.04
R6240:Vmn2r6	UTSW	3	64,464,226 (GRCm39)	missense	probably damaging	1.00
R6433:Vmn2r6	UTSW	3	64,454,801 (GRCm39)	nonsense	probably null
R6645:Vmn2r6	UTSW	3	64,464,297 (GRCm39)	missense	probably damaging	1.00
R6791:Vmn2r6	UTSW	3	64,445,580 (GRCm39)	missense	probably damaging	1.00
R7265:Vmn2r6	UTSW	3	64,464,195 (GRCm39)	missense	probably benign	0.00
R7503:Vmn2r6	UTSW	3	64,447,372 (GRCm39)	nonsense	probably null
R7562:Vmn2r6	UTSW	3	64,463,941 (GRCm39)	missense	probably benign	0.00
R7584:Vmn2r6	UTSW	3	64,472,683 (GRCm39)	missense	probably benign	0.07
R7611:Vmn2r6	UTSW	3	64,472,563 (GRCm39)	missense	probably damaging	0.98
R7759:Vmn2r6	UTSW	3	64,463,991 (GRCm39)	missense	probably damaging	1.00
R7834:Vmn2r6	UTSW	3	64,445,443 (GRCm39)	missense	probably damaging	1.00
R7933:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
R7982:Vmn2r6	UTSW	3	64,467,241 (GRCm39)	missense	probably damaging	1.00
R8024:Vmn2r6	UTSW	3	64,467,245 (GRCm39)	missense	probably benign	0.40
R8074:Vmn2r6	UTSW	3	64,455,064 (GRCm39)	intron	probably benign
R8169:Vmn2r6	UTSW	3	64,447,310 (GRCm39)	missense	probably benign	0.01
R8337:Vmn2r6	UTSW	3	64,463,526 (GRCm39)	nonsense	probably null
R8736:Vmn2r6	UTSW	3	64,467,221 (GRCm39)	missense	probably damaging	1.00
R8962:Vmn2r6	UTSW	3	64,463,576 (GRCm39)	missense	probably damaging	1.00
R9139:Vmn2r6	UTSW	3	64,464,277 (GRCm39)	missense	probably benign	0.12
R9206:Vmn2r6	UTSW	3	64,467,032 (GRCm39)	missense	probably damaging	0.97
R9295:Vmn2r6	UTSW	3	64,463,484 (GRCm39)	missense	probably benign	0.00
R9332:Vmn2r6	UTSW	3	64,454,671 (GRCm39)	missense	probably benign	0.01
R9616:Vmn2r6	UTSW	3	64,445,724 (GRCm39)	missense	probably damaging	1.00
R9663:Vmn2r6	UTSW	3	64,463,549 (GRCm39)	missense	possibly damaging	0.90
R9685:Vmn2r6	UTSW	3	64,464,081 (GRCm39)	missense	probably benign	0.19
X0020:Vmn2r6	UTSW	3	64,445,871 (GRCm39)	missense	probably benign
X0066:Vmn2r6	UTSW	3	64,454,799 (GRCm39)	missense	probably damaging	1.00
Z1176:Vmn2r6	UTSW	3	64,463,746 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GCACAATGACTTTGGCAGTGGAAC -3'
(R):5'- ACTGTCTACAGTAGGATGTGAGGGC -3'

Sequencing Primer
(F):5'- CAGTGGAACTCTTTACTGCATCAAC -3'
(R):5'- CTGTTCACCTCCTTCTCAAATATTC -3'

Posted On

2014-01-29