Mutagenetix > Incidental Mutations

Incidental Mutation 'R4723:Olfr656'

354602

Institutional Source

Beutler Lab

Gene Symbol

Olfr656

Ensembl Gene

ENSMUSG00000073924

Gene Name

olfactory receptor 656

Synonyms

GA_x6K02T2PBJ9-7245486-7246451, MOR27-1

MMRRC Submission

041959-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R4723 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

104613955-104621603 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 104618489 bp

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 270 (V270A)

Ref Sequence

ENSEMBL: ENSMUSP00000149625 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098172] [ENSMUST00000215575]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000098172
AA Change: V278A

PolyPhen 2 Score 0.559 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000095774
Gene: ENSMUSG00000073924
AA Change: V278A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	41	319	5.2e-105	PFAM
Pfam:7TM_GPCR_Srsx	42	316	1.1e-8	PFAM
Pfam:7tm_1	51	302	4.9e-19	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210687
AA Change: V270A

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215575
AA Change: V270A

PolyPhen 2 Score 0.559 (Sensitivity: 0.88; Specificity: 0.91)

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.3%

Validation Efficiency

94% (77/82)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932443I19Rik	A	T	8: 13,735,937	N69I	probably damaging	Het
Adgrv1	T	C	13: 81,433,525	D4800G	probably benign	Het
Akna	T	C	4: 63,387,032	D499G	probably benign	Het
Arid1b	A	C	17: 5,337,290	I1673L	probably benign	Het
Bcr	T	A	10: 75,175,329	M24K	probably benign	Het
Bsn	A	G	9: 108,112,655	V1966A	probably benign	Het
Ccdc39	T	C	3: 33,813,078	N928S	possibly damaging	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,712,500		probably benign	Het
Cdc6	G	A	11: 98,908,831		probably null	Het
Cmtm1	C	T	8: 104,293,675	A371T	probably damaging	Het
Cmtm7	A	C	9: 114,763,391	V46G	possibly damaging	Het
Cmtr1	A	G	17: 29,687,157		probably null	Het
Col5a3	T	C	9: 20,809,591	H149R	unknown	Het
Coro7	T	G	16: 4,631,994	Q634P	probably benign	Het
Crym	A	G	7: 120,201,075		probably null	Het
Csmd3	A	T	15: 47,669,160	F2546L	probably benign	Het
Dmap1	T	C	4: 117,676,039	T273A	probably benign	Het
Dnah1	T	C	14: 31,272,942	Y2786C	probably damaging	Het
Echs1	A	C	7: 140,110,648		probably benign	Het
Edem3	T	A	1: 151,804,698	F525I	possibly damaging	Het
Exosc3	T	C	4: 45,319,642	I127V	probably benign	Het
Fam193a	A	G	5: 34,420,786	D208G	probably benign	Het
Farp2	T	C	1: 93,580,899	V773A	probably benign	Het
Gas6	A	G	8: 13,466,848	V550A	probably damaging	Het
Gkn3	C	T	6: 87,383,525	A163T	probably damaging	Het
Gm2423	A	G	13: 13,232,376		noncoding transcript	Het
Grin1	T	C	2: 25,294,470	S911G	probably benign	Het
Hkdc1	T	C	10: 62,400,354	I470V	probably benign	Het
Hsdl2	T	A	4: 59,593,270		probably benign	Het
Iars2	A	G	1: 185,315,979	Y519H	probably damaging	Het
Ikbkb	A	T	8: 22,669,607	M455K	probably benign	Het
Keap1	G	T	9: 21,231,410	H516Q	probably benign	Het
Klk1b27	A	T	7: 44,056,532	I220F	probably damaging	Het
Knop1	G	A	7: 118,855,864		probably benign	Het
Lhcgr	T	C	17: 88,742,602	T499A	probably benign	Het
Lrch3	A	T	16: 32,988,484		probably null	Het
Lrrc2	G	A	9: 110,970,160		probably null	Het
Lrrk2	T	C	15: 91,764,759	L1652P	probably damaging	Het
Mbl1	T	C	14: 41,154,558	V71A	possibly damaging	Het
Med8	T	A	4: 118,411,801	M1K	probably null	Het
Mfsd2b	A	G	12: 4,868,992	L88P	probably benign	Het
Mkrn2	T	A	6: 115,611,850	C185S	probably damaging	Het
Myo1d	A	G	11: 80,779,841		probably benign	Het
Napg	T	G	18: 62,992,492		probably null	Het
Ncor1	A	G	11: 62,378,612	M253T	probably benign	Het
Oas3	T	C	5: 120,766,256	T518A	unknown	Het
Obox3-ps8	A	G	17: 36,453,144		noncoding transcript	Het
Olfr519	A	T	7: 108,893,821	F195L	probably benign	Het
Olfr71	T	C	4: 43,705,785	K261R	probably damaging	Het
Opn5	A	T	17: 42,607,200	M57K	probably damaging	Het
Pde2a	C	G	7: 101,494,618	P148R	possibly damaging	Het
Prss56	C	T	1: 87,185,337	L158F	possibly damaging	Het
Psmg3	G	A	5: 139,826,370		probably benign	Het
Rnase9	C	T	14: 51,039,444	G26R	probably damaging	Het
Skint4	T	C	4: 112,118,236	V131A	possibly damaging	Het
Slc10a4	T	A	5: 73,012,055	V341E	probably damaging	Het
Slc16a14	T	C	1: 84,913,020	Y188C	probably damaging	Het
Slc7a1	G	T	5: 148,335,440	P476T	probably damaging	Het
Smchd1	A	T	17: 71,436,747	C474*	probably null	Het
Smurf1	A	T	5: 144,893,184	D336E	probably damaging	Het
Sox30	G	A	11: 45,984,765	S448N	probably benign	Het
Spag11a	G	A	8: 19,159,382	V63I	possibly damaging	Het
Sprr1b	T	G	3: 92,437,293	K92T	probably damaging	Het
Stam2	T	C	2: 52,720,950	Y20C	probably benign	Het
Sult2b1	T	A	7: 45,742,065	Y97F	probably damaging	Het
Tecpr2	C	T	12: 110,932,976	P593S	probably benign	Het
Tek	T	C	4: 94,799,160	V170A	possibly damaging	Het
Tiam2	A	G	17: 3,450,317	Y891C	probably benign	Het
Tmem220	A	G	11: 67,029,993	T75A	possibly damaging	Het
Traf3	T	G	12: 111,262,036	D560E	probably damaging	Het
Txlnb	A	G	10: 17,799,267	H56R	probably benign	Het
Vmn2r98	A	T	17: 19,066,340	N367Y	probably benign	Het
Vnn3	A	G	10: 23,851,691	I36M	possibly damaging	Het
Zbtb7a	G	A	10: 81,144,440	R156H	probably damaging	Het

Other mutations in Olfr656

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01616:Olfr656	APN	7	104617721	missense	probably damaging	0.98
IGL01908:Olfr656	APN	7	104617699	missense	probably damaging	1.00
IGL02695:Olfr656	APN	7	104618471	missense	probably damaging	1.00
IGL03055:Olfr656	UTSW	7	104618206	missense	probably damaging	1.00
R0128:Olfr656	UTSW	7	104618581	missense	probably damaging	1.00
R0184:Olfr656	UTSW	7	104618240	missense	probably damaging	1.00
R4674:Olfr656	UTSW	7	104618424	nonsense	probably null
R4675:Olfr656	UTSW	7	104618424	nonsense	probably null
R4979:Olfr656	UTSW	7	104618605	missense	probably null	0.03
R6273:Olfr656	UTSW	7	104617895	missense	probably damaging	1.00
R6359:Olfr656	UTSW	7	104618303	missense	probably damaging	1.00
R6582:Olfr656	UTSW	7	104618441	missense	probably damaging	1.00
R6750:Olfr656	UTSW	7	104618113	missense	probably damaging	0.99
R7426:Olfr656	UTSW	7	104617852	missense	probably damaging	1.00
R7786:Olfr656	UTSW	7	104617718	missense	probably benign	0.02
R8068:Olfr656	UTSW	7	104618253	nonsense	probably null
X0065:Olfr656	UTSW	7	104617758	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AATCGTGTGTATGGGCTGAC -3'
(R):5'- CAGAAGCTTTTCAGAGGATCTTATC -3'

Sequencing Primer
(F):5'- CAGCCGCACTGTTGGTCATTG -3'
(R):5'- TCCAGTTTCAAATACTGAGAGTTTC -3'

Posted On

2015-10-21