Mutagenetix > Incidental Mutation

Incidental Mutation 'R6278:Vmn2r73'

507776

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

MMRRC Submission

044448-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.172) question?

Stock #

R6278 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85506755-85525146 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 85522140 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 66 (H66Q)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

AlphaFold

D3Z7M3

Predicted Effect

probably benign
Transcript: ENSMUST00000077478
AA Change: H66Q

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: H66Q

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.8%
20x: 93.6%

Validation Efficiency

100% (49/49)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsm3	T	C	7: 119,373,072 (GRCm39)	S192P	probably damaging	Het
Amy1	T	A	3: 113,355,339 (GRCm39)	Y348F	probably damaging	Het
Brpf3	C	A	17: 29,040,258 (GRCm39)	P893H	probably benign	Het
Cage1	T	C	13: 38,200,395 (GRCm39)	T702A	possibly damaging	Het
Cdk11b	A	G	4: 155,734,060 (GRCm39)		probably benign	Het
Cdk5rap3	T	C	11: 96,802,729 (GRCm39)	Y207C	probably damaging	Het
Cenpc1	T	C	5: 86,183,394 (GRCm39)	K465R	probably damaging	Het
Cldn14	A	C	16: 93,716,486 (GRCm39)	L120R	possibly damaging	Het
Cyp2c69	A	T	19: 39,831,507 (GRCm39)	C435*	probably null	Het
Dmxl2	T	A	9: 54,323,046 (GRCm39)	E1446V	probably damaging	Het
Dnah17	T	A	11: 118,017,116 (GRCm39)	D208V	probably damaging	Het
Eif4g3	G	T	4: 137,915,394 (GRCm39)	G1430V	possibly damaging	Het
Eif5	T	A	12: 111,509,227 (GRCm39)	D284E	probably benign	Het
Fam47e	T	C	5: 92,710,376 (GRCm39)	L125P	probably damaging	Het
Far1	A	G	7: 113,167,344 (GRCm39)	I476M	probably benign	Het
Fsip2	A	T	2: 82,819,242 (GRCm39)	I4992L	probably benign	Het
Gli3	T	A	13: 15,899,698 (GRCm39)	N1028K	possibly damaging	Het
Gucy1a1	T	C	3: 82,004,941 (GRCm39)	K615E	probably damaging	Het
Gykl1	G	T	18: 52,828,280 (GRCm39)	S496I	probably benign	Het
Hmcn1	C	T	1: 150,573,170 (GRCm39)		probably null	Het
Itga2	T	A	13: 114,982,424 (GRCm39)	H1027L	probably benign	Het
Kif24	A	T	4: 41,423,498 (GRCm39)	V251E	probably damaging	Het
Nupr1	T	C	7: 126,224,518 (GRCm39)	Y30C	probably damaging	Het
Or5b119	T	A	19: 13,457,119 (GRCm39)	M148L	probably benign	Het
Or5g29	A	G	2: 85,421,342 (GRCm39)	I153V	probably benign	Het
Or5w14	A	T	2: 87,541,815 (GRCm39)	L145*	probably null	Het
Or8g26	T	A	9: 39,095,594 (GRCm39)	I40N	probably damaging	Het
P2ry1	T	A	3: 60,911,215 (GRCm39)	I118N	possibly damaging	Het
Pcdh1	C	T	18: 38,332,263 (GRCm39)	V247M	probably benign	Het
Pdia5	A	G	16: 35,250,293 (GRCm39)	V222A	possibly damaging	Het
Plce1	G	A	19: 38,713,495 (GRCm39)		probably null	Het
Ppm1m	T	C	9: 106,074,427 (GRCm39)	R239G	probably damaging	Het
Pramel30	C	T	4: 144,056,837 (GRCm39)	P7S	probably damaging	Het
Prkcz	A	T	4: 155,352,652 (GRCm39)	F492I	probably damaging	Het
Rpl6	T	C	5: 121,346,912 (GRCm39)	I269T	possibly damaging	Het
Scoc	T	C	8: 84,184,965 (GRCm39)	S2G	unknown	Het
Spesp1	A	T	9: 62,179,921 (GRCm39)	M329K	probably benign	Het
Ston2	T	C	12: 91,615,104 (GRCm39)	K435E	probably damaging	Het
Synj2	A	T	17: 6,026,149 (GRCm39)	L69F	probably damaging	Het
Tigd5	T	C	15: 75,781,842 (GRCm39)	I68T	probably damaging	Het
Tmcc2	A	T	1: 132,286,720 (GRCm39)	M577K	probably damaging	Het
Tmem131l	A	T	3: 83,849,798 (GRCm39)	I263N	possibly damaging	Het
Trip13	T	C	13: 74,061,439 (GRCm39)	E408G	probably benign	Het
Txnl4b	T	C	8: 110,295,735 (GRCm39)		probably null	Het
Ube2o	T	C	11: 116,430,369 (GRCm39)	E1123G	probably damaging	Het
Vmn1r123	A	G	7: 20,896,774 (GRCm39)	H222R	possibly damaging	Het
Zc3h13	T	A	14: 75,567,863 (GRCm39)	V1052D	probably benign	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85,506,795 (GRCm39)	missense
IGL01151:Vmn2r73	APN	7	85,507,086 (GRCm39)	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85,521,455 (GRCm39)	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85,507,267 (GRCm39)	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85,506,757 (GRCm39)	makesense	probably null
IGL01818:Vmn2r73	APN	7	85,519,109 (GRCm39)	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85,521,902 (GRCm39)	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85,520,847 (GRCm39)	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85,525,007 (GRCm39)	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85,518,980 (GRCm39)	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85,507,123 (GRCm39)	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85,507,383 (GRCm39)	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85,522,084 (GRCm39)	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85,507,495 (GRCm39)	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85,519,468 (GRCm39)	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85,525,075 (GRCm39)	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R0413:Vmn2r73	UTSW	7	85,521,087 (GRCm39)	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R1523:Vmn2r73	UTSW	7	85,519,486 (GRCm39)	missense	probably benign	0.02
R1618:Vmn2r73	UTSW	7	85,525,120 (GRCm39)	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85,506,889 (GRCm39)	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85,507,375 (GRCm39)	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85,506,936 (GRCm39)	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85,506,975 (GRCm39)	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85,521,431 (GRCm39)	missense	probably damaging	1.00
R2926:Vmn2r73	UTSW	7	85,520,871 (GRCm39)	missense	probably benign	0.17
R3719:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85,521,198 (GRCm39)	missense	probably benign
R3838:Vmn2r73	UTSW	7	85,507,258 (GRCm39)	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85,507,144 (GRCm39)	missense	probably benign
R4030:Vmn2r73	UTSW	7	85,521,044 (GRCm39)	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85,521,883 (GRCm39)	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85,520,768 (GRCm39)	missense	probably damaging	0.97
R4427:Vmn2r73	UTSW	7	85,506,981 (GRCm39)	missense	probably damaging	1.00
R4430:Vmn2r73	UTSW	7	85,519,449 (GRCm39)	missense	probably benign
R4552:Vmn2r73	UTSW	7	85,525,055 (GRCm39)	missense	probably benign	0.12
R4779:Vmn2r73	UTSW	7	85,520,923 (GRCm39)	nonsense	probably null
R4906:Vmn2r73	UTSW	7	85,506,829 (GRCm39)	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r73	UTSW	7	85,507,155 (GRCm39)	missense	probably damaging	0.98
R5344:Vmn2r73	UTSW	7	85,525,046 (GRCm39)	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85,518,996 (GRCm39)	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85,507,575 (GRCm39)	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85,507,299 (GRCm39)	missense	probably damaging	1.00
R5826:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R6018:Vmn2r73	UTSW	7	85,521,875 (GRCm39)	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85,507,429 (GRCm39)	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R6233:Vmn2r73	UTSW	7	85,519,099 (GRCm39)	missense	probably benign	0.00
R6283:Vmn2r73	UTSW	7	85,521,049 (GRCm39)	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85,506,828 (GRCm39)	missense	probably damaging	1.00
R6413:Vmn2r73	UTSW	7	85,519,544 (GRCm39)	nonsense	probably null
R6782:Vmn2r73	UTSW	7	85,519,563 (GRCm39)	missense	probably benign	0.00
R6884:Vmn2r73	UTSW	7	85,507,213 (GRCm39)	missense	probably benign	0.05
R6921:Vmn2r73	UTSW	7	85,507,446 (GRCm39)	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85,507,663 (GRCm39)	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85,522,075 (GRCm39)	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85,521,192 (GRCm39)	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85,521,147 (GRCm39)	missense	probably benign
R7811:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85,507,619 (GRCm39)	missense	probably benign
R8301:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85,507,128 (GRCm39)	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85,519,638 (GRCm39)	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85,521,902 (GRCm39)	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85,521,095 (GRCm39)	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85,507,668 (GRCm39)	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85,521,179 (GRCm39)	missense	probably damaging	0.98
R8860:Vmn2r73	UTSW	7	85,522,149 (GRCm39)	splice site	probably benign
R9002:Vmn2r73	UTSW	7	85,507,284 (GRCm39)	missense	probably benign	0.32
R9008:Vmn2r73	UTSW	7	85,521,904 (GRCm39)	missense	probably damaging	0.96
R9159:Vmn2r73	UTSW	7	85,521,931 (GRCm39)	missense	possibly damaging	0.90
R9180:Vmn2r73	UTSW	7	85,507,123 (GRCm39)	missense	probably benign	0.07
R9198:Vmn2r73	UTSW	7	85,518,987 (GRCm39)	missense	probably benign	0.01
R9329:Vmn2r73	UTSW	7	85,519,073 (GRCm39)	missense	probably benign
R9448:Vmn2r73	UTSW	7	85,522,027 (GRCm39)	missense	probably benign	0.00
R9463:Vmn2r73	UTSW	7	85,506,795 (GRCm39)	missense
R9589:Vmn2r73	UTSW	7	85,519,659 (GRCm39)	missense	probably benign	0.03
X0023:Vmn2r73	UTSW	7	85,507,664 (GRCm39)	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85,521,176 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85,521,480 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTGCCCATATAGGAGCTGTAAG -3'
(R):5'- GAAGAAGGCTTGTGATAGCATTATG -3'

Sequencing Primer
(F):5'- CTCTCATTTGCACAGTAGTAATTAGG -3'
(R):5'- AAGGCTTGTGATAGCATTATGATTTG -3'

Posted On

2018-03-15