Incidental Mutation 'R7591:Zfp442'
| ID |
587391 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp442
|
| Ensembl Gene |
ENSMUSG00000068130 |
| Gene Name |
zinc finger protein 442 |
| Synonyms |
OTTMUSG00000015730 |
| MMRRC Submission |
045638-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.060)
|
| Stock # |
R7591 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
2 |
| Chromosomal Location |
150249061-150293406 bp(-) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
A to T
at 150250092 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tyrosine to Stop codon
at position 603
(Y603*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000105542
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000109916]
[ENSMUST00000185796]
|
| AlphaFold |
A2AQA0 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000109916
AA Change: Y603*
|
| SMART Domains |
Protein: ENSMUSP00000105542
Gene: ENSMUSG00000068130
AA Change: Y603*
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
4 |
66 |
3.27e-19 |
SMART |
|
ZnF_C2H2
|
159 |
181 |
8.34e-3 |
SMART |
|
ZnF_C2H2
|
211 |
233 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
239 |
261 |
2.43e-4 |
SMART |
|
ZnF_C2H2
|
267 |
289 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
295 |
317 |
4.17e-3 |
SMART |
|
ZnF_C2H2
|
323 |
345 |
3.16e-3 |
SMART |
|
ZnF_C2H2
|
351 |
373 |
1.58e-3 |
SMART |
|
ZnF_C2H2
|
379 |
401 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
407 |
429 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
435 |
457 |
2.2e-2 |
SMART |
|
ZnF_C2H2
|
463 |
485 |
1.6e-4 |
SMART |
|
ZnF_C2H2
|
491 |
513 |
1.82e-3 |
SMART |
|
ZnF_C2H2
|
519 |
541 |
4.47e-3 |
SMART |
|
ZnF_C2H2
|
547 |
569 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
575 |
597 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
603 |
625 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
631 |
654 |
3.11e-2 |
SMART |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000185796
AA Change: Y546*
|
| SMART Domains |
Protein: ENSMUSP00000140098
Gene: ENSMUSG00000068130
AA Change: Y546*
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
3 |
65 |
1.4e-21 |
SMART |
|
ZnF_C2H2
|
158 |
180 |
3.4e-5 |
SMART |
|
ZnF_C2H2
|
210 |
232 |
3.9e-5 |
SMART |
|
ZnF_C2H2
|
238 |
260 |
1e-6 |
SMART |
|
ZnF_C2H2
|
266 |
288 |
5.6e-6 |
SMART |
|
ZnF_C2H2
|
294 |
316 |
1.8e-5 |
SMART |
|
ZnF_C2H2
|
322 |
344 |
1.3e-5 |
SMART |
|
ZnF_C2H2
|
350 |
372 |
6.7e-6 |
SMART |
|
ZnF_C2H2
|
378 |
400 |
9.6e-5 |
SMART |
|
ZnF_C2H2
|
406 |
428 |
6.9e-7 |
SMART |
|
ZnF_C2H2
|
434 |
456 |
7.7e-6 |
SMART |
|
ZnF_C2H2
|
462 |
484 |
1.9e-5 |
SMART |
|
ZnF_C2H2
|
490 |
512 |
1.5e-5 |
SMART |
|
ZnF_C2H2
|
518 |
540 |
2e-5 |
SMART |
|
ZnF_C2H2
|
546 |
568 |
3.5e-6 |
SMART |
|
ZnF_C2H2
|
574 |
597 |
1.3e-4 |
SMART |
|
| Meta Mutation Damage Score |
0.9756 |
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.7%
- 20x: 99.2%
|
| Validation Efficiency |
100% (45/45) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 43 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Ccne2 |
T |
C |
4: 11,201,393 (GRCm39) |
I307T |
probably benign |
Het |
| Chrm3 |
A |
T |
13: 9,927,349 (GRCm39) |
C562* |
probably null |
Het |
| Cldn11 |
A |
T |
3: 31,204,436 (GRCm39) |
E46D |
probably benign |
Het |
| Cyp4a14 |
A |
G |
4: 115,347,157 (GRCm39) |
|
probably null |
Het |
| Dbh |
A |
G |
2: 27,060,522 (GRCm39) |
T233A |
probably damaging |
Het |
| Dennd2c |
T |
A |
3: 103,040,661 (GRCm39) |
Y309N |
possibly damaging |
Het |
| Dnttip2 |
T |
A |
3: 122,070,117 (GRCm39) |
L444* |
probably null |
Het |
| Enpp5 |
G |
A |
17: 44,396,155 (GRCm39) |
G356S |
probably damaging |
Het |
| Epop |
A |
T |
11: 97,519,158 (GRCm39) |
V317E |
probably damaging |
Het |
| Eppk1 |
T |
C |
15: 75,991,797 (GRCm39) |
T1695A |
possibly damaging |
Het |
| Fam149a |
T |
A |
8: 45,803,472 (GRCm39) |
I421F |
possibly damaging |
Het |
| Impa2 |
T |
C |
18: 67,451,480 (GRCm39) |
L258P |
probably damaging |
Het |
| Itga1 |
G |
A |
13: 115,119,315 (GRCm39) |
R855W |
probably damaging |
Het |
| Kcnh5 |
T |
C |
12: 75,054,541 (GRCm39) |
T468A |
probably benign |
Het |
| Kif11 |
A |
G |
19: 37,372,711 (GRCm39) |
K33R |
probably damaging |
Het |
| Lamb1 |
C |
A |
12: 31,376,647 (GRCm39) |
A1657E |
probably damaging |
Het |
| Macc1 |
T |
C |
12: 119,410,393 (GRCm39) |
V387A |
probably damaging |
Het |
| Nalcn |
T |
C |
14: 123,561,297 (GRCm39) |
T734A |
probably benign |
Het |
| Nphp3 |
T |
C |
9: 103,895,477 (GRCm39) |
|
probably null |
Het |
| Nutm2 |
A |
G |
13: 50,627,903 (GRCm39) |
I461M |
probably damaging |
Het |
| Or14p1 |
T |
C |
13: 65,292,462 (GRCm39) |
F130L |
probably benign |
Het |
| Or4p7 |
G |
A |
2: 88,222,220 (GRCm39) |
V210M |
probably benign |
Het |
| Or4s2 |
A |
C |
2: 88,473,811 (GRCm39) |
K233N |
probably damaging |
Het |
| Or5b112 |
A |
G |
19: 13,319,619 (GRCm39) |
S166G |
probably benign |
Het |
| Pigo |
T |
C |
4: 43,025,093 (GRCm39) |
N2S |
probably benign |
Het |
| Plcl1 |
A |
T |
1: 55,736,608 (GRCm39) |
I650L |
probably benign |
Het |
| Ppcdc |
A |
T |
9: 57,342,262 (GRCm39) |
V20D |
probably damaging |
Het |
| Pramel27 |
A |
T |
4: 143,577,481 (GRCm39) |
I88L |
probably benign |
Het |
| Rhobtb2 |
A |
G |
14: 70,037,190 (GRCm39) |
V78A |
possibly damaging |
Het |
| Rp1l1 |
T |
C |
14: 64,263,558 (GRCm39) |
V226A |
probably damaging |
Het |
| Scrt1 |
C |
A |
15: 76,403,694 (GRCm39) |
G99C |
probably damaging |
Het |
| Skint9 |
A |
T |
4: 112,248,147 (GRCm39) |
I199N |
probably damaging |
Het |
| Slc5a4a |
T |
C |
10: 75,983,501 (GRCm39) |
|
probably benign |
Het |
| Smad3 |
C |
T |
9: 63,561,999 (GRCm39) |
W326* |
probably null |
Het |
| Spata18 |
A |
T |
5: 73,829,759 (GRCm39) |
I305F |
|
Het |
| Spata31h1 |
C |
A |
10: 82,128,046 (GRCm39) |
V1655F |
probably benign |
Het |
| St3gal1 |
A |
G |
15: 66,983,195 (GRCm39) |
V187A |
possibly damaging |
Het |
| Syne3 |
A |
G |
12: 104,906,863 (GRCm39) |
|
probably null |
Het |
| Trim69 |
G |
A |
2: 121,998,454 (GRCm39) |
R142Q |
probably benign |
Het |
| Vmn1r40 |
A |
T |
6: 89,691,755 (GRCm39) |
I191F |
probably benign |
Het |
| Vmn2r16 |
T |
A |
5: 109,510,223 (GRCm39) |
D535E |
probably damaging |
Het |
| Vwa5b2 |
A |
G |
16: 20,420,317 (GRCm39) |
E742G |
probably damaging |
Het |
| Zp1 |
T |
C |
19: 10,896,835 (GRCm39) |
N68S |
probably damaging |
Het |
|
| Other mutations in Zfp442 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01081:Zfp442
|
APN |
2 |
150,251,267 (GRCm39) |
nonsense |
probably null |
|
|
IGL02566:Zfp442
|
APN |
2 |
150,251,711 (GRCm39) |
critical splice acceptor site |
probably null |
|
|
IGL03217:Zfp442
|
APN |
2 |
150,251,714 (GRCm39) |
splice site |
probably benign |
|
|
LCD18:Zfp442
|
UTSW |
2 |
150,261,768 (GRCm39) |
intron |
probably benign |
|
|
PIT4812001:Zfp442
|
UTSW |
2 |
150,251,661 (GRCm39) |
nonsense |
probably null |
|
|
R0219:Zfp442
|
UTSW |
2 |
150,253,160 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0521:Zfp442
|
UTSW |
2 |
150,253,169 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R1633:Zfp442
|
UTSW |
2 |
150,250,260 (GRCm39) |
nonsense |
probably null |
|
|
R1702:Zfp442
|
UTSW |
2 |
150,251,100 (GRCm39) |
nonsense |
probably null |
|
|
R1829:Zfp442
|
UTSW |
2 |
150,250,983 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1868:Zfp442
|
UTSW |
2 |
150,250,100 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1898:Zfp442
|
UTSW |
2 |
150,250,582 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2030:Zfp442
|
UTSW |
2 |
150,250,042 (GRCm39) |
missense |
possibly damaging |
0.58 |
|
R4676:Zfp442
|
UTSW |
2 |
150,251,526 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4717:Zfp442
|
UTSW |
2 |
150,250,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4894:Zfp442
|
UTSW |
2 |
150,253,130 (GRCm39) |
critical splice donor site |
probably null |
|
|
R4932:Zfp442
|
UTSW |
2 |
150,251,635 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R4963:Zfp442
|
UTSW |
2 |
150,250,415 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5130:Zfp442
|
UTSW |
2 |
150,251,530 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R5476:Zfp442
|
UTSW |
2 |
150,250,079 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5986:Zfp442
|
UTSW |
2 |
150,249,944 (GRCm39) |
nonsense |
probably null |
|
|
R6042:Zfp442
|
UTSW |
2 |
150,250,016 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6383:Zfp442
|
UTSW |
2 |
150,293,321 (GRCm39) |
critical splice donor site |
probably null |
|
|
R6452:Zfp442
|
UTSW |
2 |
150,250,028 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6787:Zfp442
|
UTSW |
2 |
150,251,499 (GRCm39) |
missense |
possibly damaging |
0.72 |
|
R6931:Zfp442
|
UTSW |
2 |
150,252,860 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7061:Zfp442
|
UTSW |
2 |
150,249,937 (GRCm39) |
missense |
probably benign |
0.33 |
|
R7184:Zfp442
|
UTSW |
2 |
150,250,056 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R7214:Zfp442
|
UTSW |
2 |
150,251,201 (GRCm39) |
missense |
probably benign |
0.04 |
|
R7225:Zfp442
|
UTSW |
2 |
150,250,925 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7513:Zfp442
|
UTSW |
2 |
150,250,676 (GRCm39) |
missense |
unknown |
|
|
R7679:Zfp442
|
UTSW |
2 |
150,252,917 (GRCm39) |
nonsense |
probably null |
|
|
R7768:Zfp442
|
UTSW |
2 |
150,250,241 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R7801:Zfp442
|
UTSW |
2 |
150,251,639 (GRCm39) |
missense |
probably benign |
0.28 |
|
R7814:Zfp442
|
UTSW |
2 |
150,251,402 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R7848:Zfp442
|
UTSW |
2 |
150,253,146 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R8158:Zfp442
|
UTSW |
2 |
150,251,096 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
R8192:Zfp442
|
UTSW |
2 |
150,250,629 (GRCm39) |
missense |
unknown |
|
|
R8528:Zfp442
|
UTSW |
2 |
150,250,962 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9110:Zfp442
|
UTSW |
2 |
150,250,093 (GRCm39) |
missense |
probably benign |
0.30 |
|
R9269:Zfp442
|
UTSW |
2 |
150,251,287 (GRCm39) |
missense |
probably benign |
0.19 |
|
R9371:Zfp442
|
UTSW |
2 |
150,250,676 (GRCm39) |
missense |
unknown |
|
|
R9401:Zfp442
|
UTSW |
2 |
150,251,615 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R9459:Zfp442
|
UTSW |
2 |
150,250,668 (GRCm39) |
missense |
unknown |
|
|
R9711:Zfp442
|
UTSW |
2 |
150,250,207 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
Z1177:Zfp442
|
UTSW |
2 |
150,250,399 (GRCm39) |
missense |
probably damaging |
0.98 |
|
| Predicted Primers |
PCR Primer
(F):5'- AGAAATGTACAAAGGCTTCACCATATT -3'
(R):5'- GGGTTTCAAAGTGGTCTGCAAA -3'
Sequencing Primer
(F):5'- CTCTGAAGATGTCTGACATGCCAAG -3'
(R):5'- AGGCATTTGCATGTCAAGC -3'
|
| Posted On |
2019-10-24 |
Incidental Mutation