Mutagenetix > Incidental Mutation

Incidental Mutation 'R7771:Or7e166'

598581

Institutional Source

Beutler Lab

Gene Symbol

Or7e166

Ensembl Gene

ENSMUSG00000094678

Gene Name

olfactory receptor family 7 subfamily E member 166

Synonyms

MOR146-8P, Olfr857, GA_x6K02T2PVTD-13452606-13453535

MMRRC Submission

045827-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R7771 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19624125-19625054 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19624767 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 215 (F215I)

Ref Sequence

ENSEMBL: ENSMUSP00000148617 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077023] [ENSMUST00000212013] [ENSMUST00000217450]

AlphaFold

A0A1L1SUS1

Predicted Effect

probably benign
Transcript: ENSMUST00000077023
AA Change: F215I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000076281
Gene: ENSMUSG00000094678
AA Change: F215I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.5e-49	PFAM
Pfam:7TM_GPCR_Srsx	35	304	1.5e-5	PFAM
Pfam:7tm_1	41	290	9.4e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000212013
AA Change: F215I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (56/56)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	C	T	2: 69,069,535 (GRCm39)	A1287T	probably damaging	Het
Ahnak	A	G	19: 8,992,411 (GRCm39)	K4565R	probably damaging	Het
Asb13	A	G	13: 3,699,463 (GRCm39)	Y221C	probably damaging	Het
BB014433	C	T	8: 15,092,395 (GRCm39)	V153M	probably damaging	Het
Brip1	T	C	11: 85,952,850 (GRCm39)	E977G	probably benign	Het
C3	T	A	17: 57,522,797 (GRCm39)	D1029V	probably damaging	Het
Ccdc116	T	C	16: 16,957,455 (GRCm39)	E568G	probably benign	Het
Cdhr2	T	C	13: 54,866,088 (GRCm39)	V296A	probably damaging	Het
Cenpe	A	G	3: 134,946,702 (GRCm39)		probably null	Het
Cnot1	A	G	8: 96,491,753 (GRCm39)	V357A	probably damaging	Het
Coa3	A	T	11: 101,169,641 (GRCm39)	M38K	possibly damaging	Het
Cyp2d26	T	C	15: 82,675,947 (GRCm39)	N255S	probably benign	Het
Cysrt1	T	C	2: 25,129,237 (GRCm39)	S92G	probably benign	Het
D030056L22Rik	A	G	19: 18,690,842 (GRCm39)	E52G	possibly damaging	Het
Derl1	T	C	15: 57,743,436 (GRCm39)	D97G	probably damaging	Het
Ebf3	A	G	7: 136,911,092 (GRCm39)	Y141H	probably damaging	Het
Eci1	T	A	17: 24,652,125 (GRCm39)	L49*	probably null	Het
Gzma	C	T	13: 113,234,829 (GRCm39)	C54Y	probably damaging	Het
Hydin	A	G	8: 111,291,717 (GRCm39)	N3403S	probably damaging	Het
Ide	T	C	19: 37,275,525 (GRCm39)	N495D		Het
Ighv1-23	T	A	12: 114,728,356 (GRCm39)	Q22L	probably benign	Het
Itgb2l	A	G	16: 96,228,172 (GRCm39)	C444R	probably damaging	Het
Kcnj3	C	A	2: 55,336,949 (GRCm39)	H272N	probably damaging	Het
Lrp6	C	T	6: 134,439,579 (GRCm39)	C1218Y	probably damaging	Het
Lrrd1	T	C	5: 3,916,476 (GRCm39)	M831T	possibly damaging	Het
Map3k11	A	G	19: 5,740,636 (GRCm39)	E121G	probably damaging	Het
Mzt1	A	T	14: 99,278,012 (GRCm39)	I52N	probably damaging	Het
Or1j11	T	C	2: 36,312,156 (GRCm39)	S249P	possibly damaging	Het
Or52z12	A	G	7: 103,233,297 (GRCm39)	I23V	probably benign	Het
Pcdhb17	A	C	18: 37,619,962 (GRCm39)	Y584S	possibly damaging	Het
Per3	A	T	4: 151,110,657 (GRCm39)	D380E	probably damaging	Het
Per3	A	T	4: 151,125,902 (GRCm39)	L139Q	probably damaging	Het
Pgap6	T	C	17: 26,341,047 (GRCm39)	Y717H	probably damaging	Het
Pik3cg	T	C	12: 32,254,013 (GRCm39)	H658R	probably benign	Het
Pitpnm3	G	A	11: 71,952,314 (GRCm39)	Q626*	probably null	Het
Polr3e	T	C	7: 120,539,801 (GRCm39)	V542A	probably benign	Het
Rgs22	T	A	15: 36,050,224 (GRCm39)	H866L	possibly damaging	Het
Rnf150	A	G	8: 83,590,832 (GRCm39)	E65G	probably benign	Het
Sema3f	C	A	9: 107,569,625 (GRCm39)	R100L	possibly damaging	Het
Skic3	A	G	13: 76,283,149 (GRCm39)	H792R	probably benign	Het
Smim18	T	C	8: 34,232,370 (GRCm39)	D83G	possibly damaging	Het
Snx13	T	A	12: 35,174,527 (GRCm39)	D685E	probably benign	Het
Stat5a	A	C	11: 100,754,045 (GRCm39)	N125T	probably benign	Het
Stk24	T	C	14: 121,575,045 (GRCm39)	E21G	probably damaging	Het
Tasor	A	G	14: 27,189,516 (GRCm39)	T923A	probably damaging	Het
Tlr3	C	T	8: 45,856,076 (GRCm39)	V35I	probably benign	Het
Tmprss11b	A	T	5: 86,809,554 (GRCm39)		probably null	Het
Trpm2	T	C	10: 77,768,013 (GRCm39)	I829V	probably benign	Het
Ttc39a	T	C	4: 109,288,647 (GRCm39)	Y276H	probably damaging	Het
Vmn2r98	T	A	17: 19,287,460 (GRCm39)		probably null	Het
Wdr73	G	A	7: 80,542,975 (GRCm39)	A211V	probably benign	Het
Zc3h4	T	C	7: 16,163,824 (GRCm39)	V673A	unknown	Het
Zfp382	A	G	7: 29,832,760 (GRCm39)	H137R	probably damaging	Het
Zp2	T	G	7: 119,742,865 (GRCm39)	D89A	probably damaging	Het
Zpld2	A	T	4: 133,922,754 (GRCm39)	N526K	probably benign	Het
Zranb3	A	C	1: 127,960,605 (GRCm39)	Y220D	probably damaging	Het
Zswim8	G	A	14: 20,763,048 (GRCm39)	V316I	probably damaging	Het

Other mutations in Or7e166

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01510:Or7e166	APN	9	19,624,575 (GRCm39)	missense	probably benign	0.00
IGL01919:Or7e166	APN	9	19,624,638 (GRCm39)	missense	probably benign	0.00
IGL02157:Or7e166	APN	9	19,624,585 (GRCm39)	missense	probably benign	0.07
IGL02550:Or7e166	APN	9	19,624,343 (GRCm39)	missense	possibly damaging	0.92
IGL03329:Or7e166	APN	9	19,624,597 (GRCm39)	missense	probably benign	0.16
IGL02799:Or7e166	UTSW	9	19,624,314 (GRCm39)	missense	probably damaging	0.99
R0356:Or7e166	UTSW	9	19,624,743 (GRCm39)	missense	probably damaging	1.00
R0927:Or7e166	UTSW	9	19,624,945 (GRCm39)	missense	probably benign	0.39
R1161:Or7e166	UTSW	9	19,624,476 (GRCm39)	missense	probably damaging	1.00
R1848:Or7e166	UTSW	9	19,624,386 (GRCm39)	missense	probably benign	0.01
R5191:Or7e166	UTSW	9	19,624,630 (GRCm39)	missense	probably damaging	0.98
R5216:Or7e166	UTSW	9	19,624,585 (GRCm39)	missense	probably benign	0.07
R5259:Or7e166	UTSW	9	19,624,109 (GRCm39)	splice site	probably null
R5342:Or7e166	UTSW	9	19,624,333 (GRCm39)	missense	probably damaging	1.00
R5506:Or7e166	UTSW	9	19,624,570 (GRCm39)	missense	possibly damaging	0.61
R5526:Or7e166	UTSW	9	19,624,994 (GRCm39)	nonsense	probably null
R5594:Or7e166	UTSW	9	19,624,302 (GRCm39)	missense	probably damaging	0.99
R5928:Or7e166	UTSW	9	19,625,049 (GRCm39)	missense	probably benign	0.02
R6569:Or7e166	UTSW	9	19,624,638 (GRCm39)	missense	probably benign	0.00
R6858:Or7e166	UTSW	9	19,624,765 (GRCm39)	missense	probably damaging	0.98
R7077:Or7e166	UTSW	9	19,624,428 (GRCm39)	missense	probably benign
R7378:Or7e166	UTSW	9	19,624,183 (GRCm39)	missense	probably damaging	1.00
R8038:Or7e166	UTSW	9	19,624,976 (GRCm39)	missense	possibly damaging	0.52
R8160:Or7e166	UTSW	9	19,624,085 (GRCm39)	intron	probably benign
R8223:Or7e166	UTSW	9	19,624,705 (GRCm39)	missense	probably benign
R8400:Or7e166	UTSW	9	19,624,389 (GRCm39)	missense	probably benign	0.45
R8780:Or7e166	UTSW	9	19,624,653 (GRCm39)	missense	possibly damaging	0.92
R8946:Or7e166	UTSW	9	19,624,885 (GRCm39)	missense	probably damaging	0.99
R9164:Or7e166	UTSW	9	19,624,954 (GRCm39)	missense	probably benign	0.25
R9475:Or7e166	UTSW	9	19,624,939 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AAGCCCTTGCCTCTGTAGATG -3'
(R):5'- GACCATACTTTCCCTGGAAGAAC -3'

Sequencing Primer
(F):5'- CCCTTGCCTCTGTAGATGTTTGG -3'
(R):5'- TTCCCTGGAAGAACTAGAAACTGTG -3'

Posted On

2019-11-26