Mutagenetix > Incidental Mutation

Incidental Mutation 'R7802:Vmn2r25'

600565

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r25

Ensembl Gene

ENSMUSG00000094672

Gene Name

vomeronasal 2, receptor 25

Synonyms

EG545874

MMRRC Submission

045857-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R7802 (G1)

Quality Score

210.009

Status

Validated

Chromosome

Chromosomal Location

123799773-123830149 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 123828791 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 161 (I161N)

Ref Sequence

ENSEMBL: ENSMUSP00000124342 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000162046]

AlphaFold

W4VSP2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000162046
AA Change: I161N

PolyPhen 2 Score 0.879 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000124342
Gene: ENSMUSG00000094672
AA Change: I161N

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	473	6e-31	PFAM
Pfam:NCD3G	519	572	5.8e-25	PFAM
Pfam:7tm_3	603	840	4.8e-55	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

97% (35/36)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abl1	T	C	2: 31,650,438 (GRCm39)	V12A	probably benign	Het
Bahcc1	T	C	11: 120,165,518 (GRCm39)	F983S	probably benign	Het
Cecr2	T	C	6: 120,720,808 (GRCm39)	I312T	probably benign	Het
Col6a2	A	T	10: 76,439,632 (GRCm39)	W711R	probably damaging	Het
Epb41l4a	A	G	18: 33,961,227 (GRCm39)	F436L	probably benign	Het
Epha1	C	T	6: 42,338,875 (GRCm39)	R641Q	possibly damaging	Het
Ercc6	C	A	14: 32,239,260 (GRCm39)	A116E	probably damaging	Het
Ermard	A	G	17: 15,281,423 (GRCm39)	E611G	probably benign	Het
Galnt16	T	G	12: 80,628,021 (GRCm39)	I239S	probably damaging	Het
Gna15	T	C	10: 81,350,175 (GRCm39)	R76G	probably benign	Het
Herc2	A	G	7: 55,813,838 (GRCm39)	Y2657C	probably damaging	Het
Mapkapk2	T	C	1: 130,984,639 (GRCm39)	I238V	possibly damaging	Het
Med13l	T	C	5: 118,866,655 (GRCm39)	S570P	probably benign	Het
Mrap	C	T	16: 90,546,247 (GRCm39)	T112M	probably benign	Het
Nadsyn1	T	C	7: 143,359,763 (GRCm39)	Q403R	probably benign	Het
Nostrin	T	C	2: 69,019,356 (GRCm39)	V467A	probably benign	Het
Palb2	A	T	7: 121,710,119 (GRCm39)		probably null	Het
Parp16	A	G	9: 65,137,179 (GRCm39)	N135S	probably benign	Het
Pcnt	A	T	10: 76,211,137 (GRCm39)		probably null	Het
Pde8b	T	C	13: 95,237,446 (GRCm39)	D116G	probably damaging	Het
Pierce1	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	2: 28,356,122 (GRCm39)		probably benign	Het
Psmc5	T	C	11: 106,152,538 (GRCm39)		probably null	Het
Rsf1	G	T	7: 97,310,979 (GRCm39)	V570F		Het
Rundc3a	A	G	11: 102,290,835 (GRCm39)	E306G	probably benign	Het
Serpinb6b	C	T	13: 33,155,579 (GRCm39)			Het
Setx	A	G	2: 29,037,033 (GRCm39)	T1173A	probably benign	Het
Slamf8	A	G	1: 172,415,677 (GRCm39)	S54P	probably damaging	Het
Slc5a2	A	C	7: 127,870,970 (GRCm39)	D570A	possibly damaging	Het
Slco5a1	T	A	1: 13,060,700 (GRCm39)	Q7L	possibly damaging	Het
Stt3b	G	T	9: 115,105,949 (GRCm39)	S175R	probably damaging	Het
Taar6	T	A	10: 23,861,151 (GRCm39)	I132F	probably benign	Het
Tenm3	A	G	8: 48,689,500 (GRCm39)	V2029A	probably damaging	Het
Tgm4	A	G	9: 122,880,401 (GRCm39)		probably benign	Het
Tmt1a	A	T	15: 100,203,182 (GRCm39)	N152I	possibly damaging	Het
Togaram1	T	A	12: 65,013,758 (GRCm39)	C336*	probably null	Het
Ttn	G	A	2: 76,768,015 (GRCm39)	T3028M	unknown	Het
Vwf	T	C	6: 125,643,640 (GRCm39)	C2394R		Het

Other mutations in Vmn2r25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Vmn2r25	APN	6	123,830,130 (GRCm39)	missense	probably benign	0.25
IGL01781:Vmn2r25	APN	6	123,816,324 (GRCm39)	missense	possibly damaging	0.48
IGL01843:Vmn2r25	APN	6	123,829,962 (GRCm39)	missense	possibly damaging	0.67
IGL02023:Vmn2r25	APN	6	123,816,388 (GRCm39)	missense	probably damaging	0.96
IGL02502:Vmn2r25	APN	6	123,816,392 (GRCm39)	missense	probably damaging	0.96
IGL02709:Vmn2r25	APN	6	123,816,723 (GRCm39)	missense	possibly damaging	0.50
IGL03053:Vmn2r25	APN	6	123,800,077 (GRCm39)	missense	probably damaging	1.00
PIT4468001:Vmn2r25	UTSW	6	123,816,557 (GRCm39)	missense	probably benign	0.00
PIT4812001:Vmn2r25	UTSW	6	123,800,447 (GRCm39)	missense	probably damaging	1.00
R0054:Vmn2r25	UTSW	6	123,829,984 (GRCm39)	missense	probably benign	0.00
R0312:Vmn2r25	UTSW	6	123,805,539 (GRCm39)	splice site	probably benign
R0366:Vmn2r25	UTSW	6	123,800,581 (GRCm39)	nonsense	probably null
R0390:Vmn2r25	UTSW	6	123,800,140 (GRCm39)	missense	probably damaging	1.00
R0466:Vmn2r25	UTSW	6	123,829,008 (GRCm39)	missense	probably benign	0.16
R0541:Vmn2r25	UTSW	6	123,816,786 (GRCm39)	missense	probably damaging	0.97
R0612:Vmn2r25	UTSW	6	123,816,481 (GRCm39)	missense	probably damaging	1.00
R0865:Vmn2r25	UTSW	6	123,829,976 (GRCm39)	missense	probably benign	0.09
R1219:Vmn2r25	UTSW	6	123,816,282 (GRCm39)	missense	probably benign	0.00
R1240:Vmn2r25	UTSW	6	123,828,864 (GRCm39)	missense	probably damaging	0.98
R1701:Vmn2r25	UTSW	6	123,828,754 (GRCm39)	splice site	probably null
R1780:Vmn2r25	UTSW	6	123,805,424 (GRCm39)	missense	probably damaging	1.00
R1809:Vmn2r25	UTSW	6	123,802,337 (GRCm39)	missense	probably benign	0.00
R1833:Vmn2r25	UTSW	6	123,816,643 (GRCm39)	missense	probably benign	0.01
R1964:Vmn2r25	UTSW	6	123,800,254 (GRCm39)	missense	possibly damaging	0.94
R2154:Vmn2r25	UTSW	6	123,816,805 (GRCm39)	missense	probably benign	0.01
R2164:Vmn2r25	UTSW	6	123,816,518 (GRCm39)	missense	possibly damaging	0.96
R3799:Vmn2r25	UTSW	6	123,830,143 (GRCm39)	missense	probably benign	0.12
R3836:Vmn2r25	UTSW	6	123,830,044 (GRCm39)	missense	probably damaging	1.00
R3946:Vmn2r25	UTSW	6	123,817,057 (GRCm39)	missense	probably damaging	0.97
R4282:Vmn2r25	UTSW	6	123,800,606 (GRCm39)	missense	probably damaging	1.00
R4367:Vmn2r25	UTSW	6	123,805,496 (GRCm39)	missense	probably damaging	1.00
R4438:Vmn2r25	UTSW	6	123,816,756 (GRCm39)	missense	probably benign	0.03
R4488:Vmn2r25	UTSW	6	123,799,819 (GRCm39)	missense	probably damaging	1.00
R4580:Vmn2r25	UTSW	6	123,799,982 (GRCm39)	missense	possibly damaging	0.46
R4631:Vmn2r25	UTSW	6	123,829,962 (GRCm39)	missense	possibly damaging	0.94
R4765:Vmn2r25	UTSW	6	123,800,182 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r25	UTSW	6	123,805,406 (GRCm39)	missense	probably benign
R5207:Vmn2r25	UTSW	6	123,817,062 (GRCm39)	missense	probably damaging	1.00
R5254:Vmn2r25	UTSW	6	123,802,277 (GRCm39)	missense	probably damaging	1.00
R5444:Vmn2r25	UTSW	6	123,805,451 (GRCm39)	missense	probably benign	0.00
R5586:Vmn2r25	UTSW	6	123,802,255 (GRCm39)	missense	probably damaging	1.00
R5607:Vmn2r25	UTSW	6	123,805,318 (GRCm39)	missense	possibly damaging	0.49
R5985:Vmn2r25	UTSW	6	123,800,587 (GRCm39)	missense	probably benign
R6046:Vmn2r25	UTSW	6	123,799,876 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r25	UTSW	6	123,799,900 (GRCm39)	missense	possibly damaging	0.69
R6569:Vmn2r25	UTSW	6	123,828,941 (GRCm39)	missense	probably benign	0.01
R6826:Vmn2r25	UTSW	6	123,800,071 (GRCm39)	missense	probably damaging	1.00
R7054:Vmn2r25	UTSW	6	123,800,569 (GRCm39)	missense	probably damaging	1.00
R7120:Vmn2r25	UTSW	6	123,805,394 (GRCm39)	missense	possibly damaging	0.51
R7177:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7287:Vmn2r25	UTSW	6	123,829,040 (GRCm39)	missense	possibly damaging	0.49
R7397:Vmn2r25	UTSW	6	123,800,498 (GRCm39)	missense	probably damaging	0.96
R7486:Vmn2r25	UTSW	6	123,800,101 (GRCm39)	missense	probably damaging	1.00
R7699:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7700:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7759:Vmn2r25	UTSW	6	123,800,339 (GRCm39)	missense	probably damaging	0.99
R7850:Vmn2r25	UTSW	6	123,805,431 (GRCm39)	missense	probably damaging	1.00
R8064:Vmn2r25	UTSW	6	123,800,581 (GRCm39)	nonsense	probably null
R8170:Vmn2r25	UTSW	6	123,829,976 (GRCm39)	missense	probably benign	0.09
R8340:Vmn2r25	UTSW	6	123,829,972 (GRCm39)	missense	probably benign	0.01
R8346:Vmn2r25	UTSW	6	123,802,350 (GRCm39)	missense	probably benign	0.00
R8395:Vmn2r25	UTSW	6	123,799,982 (GRCm39)	missense	possibly damaging	0.81
R8889:Vmn2r25	UTSW	6	123,800,569 (GRCm39)	missense	probably damaging	1.00
R9094:Vmn2r25	UTSW	6	123,805,391 (GRCm39)	missense	probably benign	0.44
R9204:Vmn2r25	UTSW	6	123,830,092 (GRCm39)	missense	probably benign	0.00
R9253:Vmn2r25	UTSW	6	123,816,960 (GRCm39)	missense	probably damaging	0.98
R9392:Vmn2r25	UTSW	6	123,816,937 (GRCm39)	missense	probably benign
R9520:Vmn2r25	UTSW	6	123,830,066 (GRCm39)	nonsense	probably null
R9525:Vmn2r25	UTSW	6	123,800,164 (GRCm39)	missense	probably damaging	1.00
R9622:Vmn2r25	UTSW	6	123,816,579 (GRCm39)	missense	probably damaging	0.99
X0020:Vmn2r25	UTSW	6	123,816,359 (GRCm39)	missense	possibly damaging	0.95
Z1176:Vmn2r25	UTSW	6	123,799,856 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGTGGGCTACATAGGAAGTTC -3'
(R):5'- AATAAGTCCCTGGGATTCTTTCTC -3'

Sequencing Primer
(F):5'- GTGGGCTACATAGGAAGTTCAATATC -3'
(R):5'- GGGATTCTTTCTCTTCAATGTCAAC -3'

Posted On

2019-11-26