Mutagenetix > Incidental Mutation

Incidental Mutation 'R8440:Vmn2r61'

660385

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r61

Ensembl Gene

ENSMUSG00000090967

Gene Name

vomeronasal 2, receptor 61

Synonyms

Gprc2a-rs2, Casr-rs2, EG637873

MMRRC Submission

067884-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R8440 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41909477-41950179 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 41916080 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Valine at position 231 (E231V)

Ref Sequence

ENSEMBL: ENSMUSP00000129576 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166131]

AlphaFold

L7N2B8

Predicted Effect

probably benign
Transcript: ENSMUST00000166131
AA Change: E231V

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000129576
Gene: ENSMUSG00000090967
AA Change: E231V

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	4e-42	PFAM
Pfam:NCD3G	514	567	1.9e-21	PFAM
Pfam:7tm_3	600	835	6.2e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

97% (38/39)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ak5	G	C	3: 152,209,680 (GRCm39)		probably null	Het
Bbs9	T	A	9: 22,479,046 (GRCm39)	V163E	probably damaging	Het
Bsn	G	A	9: 107,988,651 (GRCm39)	A2367V	probably benign	Het
Cbr3	T	C	16: 93,480,421 (GRCm39)	I70T	probably damaging	Het
Chka	T	C	19: 3,943,375 (GRCm39)	Y426H	probably damaging	Het
Cpq	T	A	15: 33,213,341 (GRCm39)	M120K	possibly damaging	Het
Dcaf1	T	A	9: 106,725,073 (GRCm39)	C520S	possibly damaging	Het
Egfr	T	C	11: 16,859,831 (GRCm39)	Y1018H	probably damaging	Het
Eif3l	T	C	15: 78,961,120 (GRCm39)	Y58H	possibly damaging	Het
Frmd8	T	C	19: 5,915,202 (GRCm39)	Y234C	possibly damaging	Het
Fus	T	C	7: 127,568,998 (GRCm39)	Y50H	unknown	Het
Hmcn1	T	A	1: 150,570,671 (GRCm39)	Q2164L	probably damaging	Het
Hmcn2	C	A	2: 31,281,088 (GRCm39)	L1867I	probably benign	Het
Hsd11b1	T	C	1: 192,904,420 (GRCm39)	K260E	probably benign	Het
Igkv4-91	C	T	6: 68,745,556 (GRCm39)	G114D	probably benign	Het
Inpp4b	G	A	8: 82,768,524 (GRCm39)	E678K	probably damaging	Het
Kifc3	G	T	8: 95,836,422 (GRCm39)	P113Q	possibly damaging	Het
Lgi4	T	A	7: 30,760,049 (GRCm39)		probably null	Het
Map1s	A	G	8: 71,365,163 (GRCm39)	N107D	probably damaging	Het
Meiob	G	T	17: 25,037,302 (GRCm39)	C60F	probably benign	Het
Nedd4l	G	T	18: 65,022,126 (GRCm39)		probably null	Het
Omp	A	G	7: 97,794,253 (GRCm39)	F125L	probably damaging	Het
Or1ad6	T	C	11: 50,860,024 (GRCm39)	Y60H	probably damaging	Het
Or51h1	G	A	7: 102,308,275 (GRCm39)	M82I	probably damaging	Het
Or5a1	C	T	19: 12,097,308 (GRCm39)	C244Y	probably damaging	Het
Pcdha3	T	C	18: 37,080,914 (GRCm39)	V552A	probably damaging	Het
Plek	T	C	11: 16,945,276 (GRCm39)	D18G	possibly damaging	Het
Ppip5k2	T	C	1: 97,675,276 (GRCm39)	T376A	probably damaging	Het
Prkdc	CAAA	CAAAA	16: 15,653,022 (GRCm39)	3833	probably null	Het
Rabgap1	T	C	2: 37,432,692 (GRCm39)		probably null	Het
Rtn1	T	A	12: 72,270,173 (GRCm39)	I113F	probably damaging	Het
Ssu2	T	A	6: 112,364,950 (GRCm39)	D4V	probably benign	Het
St6galnac2	T	C	11: 116,568,374 (GRCm39)	H363R	probably damaging	Het
Stk19	T	C	17: 35,055,456 (GRCm39)	S60G	possibly damaging	Het
Suco	G	A	1: 161,679,907 (GRCm39)	T332I	probably damaging	Het
Trbv20	T	A	6: 41,165,892 (GRCm39)	Y106N	probably damaging	Het
Uck1	C	A	2: 32,150,153 (GRCm39)		probably benign	Het
Vmn1r128	G	A	7: 21,083,745 (GRCm39)	V150I	probably benign	Het
Vmn2r54	A	T	7: 12,350,013 (GRCm39)	V523E	possibly damaging	Het
Xkr9	A	G	1: 13,771,603 (GRCm39)	D373G	probably benign	Het
Zfp592	G	C	7: 80,691,271 (GRCm39)	S1150T	possibly damaging	Het

Other mutations in Vmn2r61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r61	APN	7	41,950,175 (GRCm39)	missense	possibly damaging	0.96
IGL00824:Vmn2r61	APN	7	41,916,438 (GRCm39)	missense	probably benign	0.03
IGL00903:Vmn2r61	APN	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
IGL01125:Vmn2r61	APN	7	41,909,550 (GRCm39)	missense	probably damaging	1.00
IGL01393:Vmn2r61	APN	7	41,916,258 (GRCm39)	missense	probably benign	0.08
IGL01712:Vmn2r61	APN	7	41,909,661 (GRCm39)	missense	probably damaging	0.98
IGL01822:Vmn2r61	APN	7	41,950,130 (GRCm39)	missense	probably benign	0.18
IGL01835:Vmn2r61	APN	7	41,950,015 (GRCm39)	missense	probably benign	0.12
IGL01844:Vmn2r61	APN	7	41,909,639 (GRCm39)	missense	probably benign	0.00
IGL01953:Vmn2r61	APN	7	41,949,613 (GRCm39)	missense	probably damaging	1.00
IGL02032:Vmn2r61	APN	7	41,949,466 (GRCm39)	missense	probably damaging	0.99
IGL02054:Vmn2r61	APN	7	41,926,158 (GRCm39)	critical splice donor site	probably null
IGL02569:Vmn2r61	APN	7	41,926,070 (GRCm39)	missense	probably damaging	1.00
IGL02697:Vmn2r61	APN	7	41,924,892 (GRCm39)	missense	possibly damaging	0.55
IGL02958:Vmn2r61	APN	7	41,949,361 (GRCm39)	missense	probably benign
IGL03290:Vmn2r61	APN	7	41,915,408 (GRCm39)	missense	probably benign	0.00
IGL03337:Vmn2r61	APN	7	41,916,509 (GRCm39)	missense	possibly damaging	0.58
IGL03369:Vmn2r61	APN	7	41,909,517 (GRCm39)	missense	probably benign
IGL03402:Vmn2r61	APN	7	41,909,679 (GRCm39)	missense	probably benign
R0026:Vmn2r61	UTSW	7	41,924,898 (GRCm39)	missense	possibly damaging	0.64
R0319:Vmn2r61	UTSW	7	41,949,941 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r61	UTSW	7	41,915,335 (GRCm39)	missense	probably benign	0.02
R0555:Vmn2r61	UTSW	7	41,915,442 (GRCm39)	missense	probably benign	0.02
R0691:Vmn2r61	UTSW	7	41,949,844 (GRCm39)	missense	probably damaging	1.00
R1701:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R1718:Vmn2r61	UTSW	7	41,950,121 (GRCm39)	missense	probably benign
R1835:Vmn2r61	UTSW	7	41,916,076 (GRCm39)	nonsense	probably null
R1920:Vmn2r61	UTSW	7	41,949,710 (GRCm39)	missense	possibly damaging	0.73
R2069:Vmn2r61	UTSW	7	41,949,425 (GRCm39)	missense	probably benign	0.06
R2326:Vmn2r61	UTSW	7	41,916,287 (GRCm39)	missense	probably damaging	1.00
R2402:Vmn2r61	UTSW	7	41,949,529 (GRCm39)	missense	possibly damaging	0.90
R3103:Vmn2r61	UTSW	7	41,916,067 (GRCm39)	missense	possibly damaging	0.73
R3107:Vmn2r61	UTSW	7	41,916,491 (GRCm39)	missense	possibly damaging	0.82
R4426:Vmn2r61	UTSW	7	41,950,159 (GRCm39)	missense	probably benign
R4426:Vmn2r61	UTSW	7	41,950,157 (GRCm39)	missense	probably benign
R4484:Vmn2r61	UTSW	7	41,950,120 (GRCm39)	missense	probably benign
R4748:Vmn2r61	UTSW	7	41,916,565 (GRCm39)	missense	probably damaging	0.96
R4835:Vmn2r61	UTSW	7	41,916,459 (GRCm39)	missense	possibly damaging	0.52
R4863:Vmn2r61	UTSW	7	41,950,132 (GRCm39)	missense	probably benign	0.03
R4923:Vmn2r61	UTSW	7	41,916,520 (GRCm39)	missense	probably damaging	1.00
R4968:Vmn2r61	UTSW	7	41,949,478 (GRCm39)	missense	probably benign	0.14
R5114:Vmn2r61	UTSW	7	41,949,953 (GRCm39)	missense	possibly damaging	0.92
R5297:Vmn2r61	UTSW	7	41,909,646 (GRCm39)	missense	probably benign
R5497:Vmn2r61	UTSW	7	41,924,906 (GRCm39)	missense	possibly damaging	0.95
R5508:Vmn2r61	UTSW	7	41,916,242 (GRCm39)	missense	possibly damaging	0.52
R5587:Vmn2r61	UTSW	7	41,949,911 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,916,677 (GRCm39)	missense	probably benign	0.00
R5782:Vmn2r61	UTSW	7	41,949,253 (GRCm39)	missense	probably damaging	1.00
R6136:Vmn2r61	UTSW	7	41,916,455 (GRCm39)	missense	probably damaging	1.00
R6207:Vmn2r61	UTSW	7	41,909,616 (GRCm39)	missense	probably benign	0.01
R6265:Vmn2r61	UTSW	7	41,915,915 (GRCm39)	missense	probably benign	0.01
R6272:Vmn2r61	UTSW	7	41,949,242 (GRCm39)	missense	probably damaging	1.00
R6355:Vmn2r61	UTSW	7	41,916,659 (GRCm39)	missense	probably benign	0.00
R6469:Vmn2r61	UTSW	7	41,915,283 (GRCm39)	nonsense	probably null
R6554:Vmn2r61	UTSW	7	41,926,139 (GRCm39)	missense	probably damaging	1.00
R6699:Vmn2r61	UTSW	7	41,949,580 (GRCm39)	missense	probably benign
R6768:Vmn2r61	UTSW	7	41,949,748 (GRCm39)	missense	probably damaging	1.00
R6824:Vmn2r61	UTSW	7	41,949,403 (GRCm39)	missense	probably benign	0.10
R6930:Vmn2r61	UTSW	7	41,949,364 (GRCm39)	missense	probably benign	0.02
R7053:Vmn2r61	UTSW	7	41,916,557 (GRCm39)	missense	probably damaging	0.96
R7238:Vmn2r61	UTSW	7	41,916,629 (GRCm39)	missense	possibly damaging	0.73
R7332:Vmn2r61	UTSW	7	41,909,534 (GRCm39)	missense	probably benign	0.00
R7359:Vmn2r61	UTSW	7	41,915,407 (GRCm39)	missense	probably benign	0.11
R7553:Vmn2r61	UTSW	7	41,916,205 (GRCm39)	missense	not run
R7710:Vmn2r61	UTSW	7	41,916,472 (GRCm39)	missense	probably damaging	1.00
R7732:Vmn2r61	UTSW	7	41,916,097 (GRCm39)	missense	probably benign
R7839:Vmn2r61	UTSW	7	41,916,032 (GRCm39)	missense	probably damaging	0.97
R7916:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R8026:Vmn2r61	UTSW	7	41,916,141 (GRCm39)	missense	probably benign	0.02
R8499:Vmn2r61	UTSW	7	41,949,700 (GRCm39)	missense	probably damaging	0.99
R8771:Vmn2r61	UTSW	7	41,916,194 (GRCm39)	missense	probably damaging	0.99
R8847:Vmn2r61	UTSW	7	41,950,010 (GRCm39)	missense	probably damaging	1.00
R8986:Vmn2r61	UTSW	7	41,915,325 (GRCm39)	nonsense	probably null
R9290:Vmn2r61	UTSW	7	41,915,385 (GRCm39)	missense	probably benign	0.27
R9311:Vmn2r61	UTSW	7	41,950,092 (GRCm39)	missense	possibly damaging	0.92
R9324:Vmn2r61	UTSW	7	41,916,619 (GRCm39)	missense	probably benign	0.00
R9476:Vmn2r61	UTSW	7	41,949,593 (GRCm39)	missense	probably damaging	1.00
R9521:Vmn2r61	UTSW	7	41,916,626 (GRCm39)	missense	probably damaging	0.99
R9619:Vmn2r61	UTSW	7	41,926,136 (GRCm39)	missense	probably damaging	0.98
R9729:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r61	UTSW	7	41,949,388 (GRCm39)	missense	possibly damaging	0.93
Z1176:Vmn2r61	UTSW	7	41,916,166 (GRCm39)	missense	probably benign	0.00
Z1176:Vmn2r61	UTSW	7	41,909,585 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- TGATTCTATCCTGAATGAACGAGG -3'
(R):5'- AACATCCCATGAAGAGGTCAAG -3'

Sequencing Primer
(F):5'- GAACGAGGTCAGTTTAATTCTCTG -3'
(R):5'- ACAGTTGTGCAATATGTCGC -3'

Posted On

2021-01-18