|Institutional Source||Beutler Lab|
|Gene Name||Ras association (RalGDS/AF-6) and pleckstrin homology domains 1|
|Synonyms||C730009O10Rik, Lpd, 9430025M21Rik, lamellipodin|
|Essential gene?||Probably non essential (E-score: 0.120)|
|Stock #||R9146 (G1)|
|Chromosomal Location||60482292-60567104 bp(-) (GRCm38)|
|Type of Mutation||critical splice donor site (2 bp from exon)|
|DNA Base Change (assembly)||A to G at 60518978 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000027168 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000027168] [ENSMUST00000090293] [ENSMUST00000140485]|
|Coding Region Coverage||
|Validation Efficiency||98% (54/55)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to the Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of adapter proteins, which function in cell migration. Members of this family contain pleckstrin-homology domains, Ras-association domains, and proline-rich C-termini. The protein encoded by this gene regulates actin dynamics through interaction with Ena/Vasodilator proteins as well as direct binding to filamentous actin to regulate actin network assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]
PHENOTYPE: Mice homozygous for a conditional allele activated in all cells exhibit background sensitive neonatal or postnatal lethality, decreased body size, belly spotting and decreased melanocyte numbers in the trunk. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Raph1||
(F):5'- CACTGTACGGAAAGTCTTTGAG -3'
(R):5'- AGTGATCCATTTCTCTTGTGCATG -3'
(F):5'- TGACTGTCCTCGAACTCAGAAATCTG -3'
(R):5'- CTCTTGTGCATGAGTAGGAGACC -3'