Mutagenetix > Incidental Mutation

Incidental Mutation 'R1775:Ank2'

204306

Institutional Source

Beutler Lab

Gene Symbol

Ank2

Ensembl Gene

ENSMUSG00000032826

Gene Name

ankyrin 2, brain

Synonyms

Ankyrin-B, Ank-2, Ankyrin-2, Gm4392, ankyrin B

MMRRC Submission

039806-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1775 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

126921612-127499350 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 126934547 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 799 (T799M)

Ref Sequence

ENSEMBL: ENSMUSP00000043765 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044443] [ENSMUST00000182064] [ENSMUST00000182078]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000044443
AA Change: T799M

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000043765
Gene: ENSMUSG00000032826
AA Change: T799M

Domain	Start	End	E-Value	Type
low complexity region	9	22	N/A	INTRINSIC
low complexity region	57	69	N/A	INTRINSIC
ZU5	128	232	4.13e-61	SMART
Pfam:ZU5	289	374	2.8e-8	PFAM
low complexity region	587	597	N/A	INTRINSIC
DEATH	603	697	1.52e-27	SMART
low complexity region	732	748	N/A	INTRINSIC
low complexity region	860	873	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000182062

Predicted Effect

unknown
Transcript: ENSMUST00000182064
AA Change: T3736M

SMART Domains

Protein: ENSMUSP00000138620
Gene: ENSMUSG00000032826
AA Change: T3736M

Domain	Start	End	E-Value	Type
ANK	9	38	1e1	SMART
ANK	42	71	8.9e-7	SMART
ANK	75	104	4.4e-9	SMART
ANK	108	137	2.8e-9	SMART
ANK	141	169	5.3e-1	SMART
ANK	170	199	7.3e-1	SMART
ANK	211	240	1.1e-7	SMART
ANK	244	273	4.4e-9	SMART
ANK	277	306	9.3e-8	SMART
ANK	310	339	2.1e-8	SMART
ANK	343	372	1.3e-7	SMART
ANK	376	405	6.2e-9	SMART
ANK	409	438	1.1e-7	SMART
ANK	442	471	2.9e-8	SMART
ANK	475	504	1.1e-5	SMART
ANK	508	537	6.5e-6	SMART
ANK	541	570	2.3e-7	SMART
ANK	574	603	2.4e-7	SMART
ANK	607	636	3.2e-9	SMART
ANK	640	669	5.5e-5	SMART
ANK	673	702	1.9e-8	SMART
ANK	706	735	3.3e-9	SMART
low complexity region	755	775	N/A	INTRINSIC
low complexity region	793	806	N/A	INTRINSIC
low complexity region	841	853	N/A	INTRINSIC
ZU5	912	1016	2e-63	SMART
low complexity region	1371	1381	N/A	INTRINSIC
low complexity region	1448	1463	N/A	INTRINSIC
low complexity region	1490	1503	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000182078
AA Change: T3649M

SMART Domains

Protein: ENSMUSP00000138753
Gene: ENSMUSG00000032826
AA Change: T3649M

Domain	Start	End	E-Value	Type
low complexity region	7	18	N/A	INTRINSIC
low complexity region	114	125	N/A	INTRINSIC
low complexity region	191	209	N/A	INTRINSIC
low complexity region	304	312	N/A	INTRINSIC
low complexity region	478	493	N/A	INTRINSIC
low complexity region	527	544	N/A	INTRINSIC
DEATH	591	685	1e-29	SMART
low complexity region	720	736	N/A	INTRINSIC
low complexity region	848	861	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000182118

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182271

Predicted Effect

probably benign
Transcript: ENSMUST00000182571

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.5%
20x: 92.9%

Validation Efficiency

98% (95/97)

MGI Phenotype

PHENOTYPE: Homozygous mutation of this gene results in death by postnatal day 8, although some animals survive to P20. Mutant animals display reduced body size, impaired balance and locomotion, brain structure dysmorphologies, abnormal lens, and optic nerve degeneration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034J04Rik	A	G	12: 11,222,142 (GRCm38)	S20P	unknown	Het
4921528I07Rik	G	A	9: 114,279,318 (GRCm38)		noncoding transcript	Het
4930562C15Rik	G	A	16: 4,851,558 (GRCm38)		probably null	Het
4933427I04Rik	A	G	4: 123,860,493 (GRCm38)	T67A	possibly damaging	Het
Abcc2	A	T	19: 43,798,419 (GRCm38)	N160Y	possibly damaging	Het
Acaca	A	G	11: 84,300,422 (GRCm38)	D155G	possibly damaging	Het
Afg3l1	G	A	8: 123,492,900 (GRCm38)	R389Q	possibly damaging	Het
Anxa2	T	C	9: 69,488,081 (GRCm38)	Y202H	possibly damaging	Het
Arhgef33	A	G	17: 80,373,743 (GRCm38)	T771A	probably benign	Het
Atp12a	T	C	14: 56,372,589 (GRCm38)	V182A	probably benign	Het
Birc6	T	C	17: 74,612,286 (GRCm38)	L210S	probably damaging	Het
Car3	A	G	3: 14,864,432 (GRCm38)	T73A	probably benign	Het
Cd5l	T	C	3: 87,368,659 (GRCm38)	L312P	probably damaging	Het
Celf5	A	G	10: 81,467,304 (GRCm38)		probably benign	Het
Cep290	T	A	10: 100,496,810 (GRCm38)	V257E	probably damaging	Het
Cntrob	T	C	11: 69,320,867 (GRCm38)	D177G	possibly damaging	Het
Copg2	A	T	6: 30,810,336 (GRCm38)	F658I	probably damaging	Het
Csmd3	T	C	15: 47,899,739 (GRCm38)	T1234A	probably damaging	Het
Dhx35	A	G	2: 158,806,437 (GRCm38)	T72A	probably damaging	Het
Dmrtc2	A	T	7: 24,874,367 (GRCm38)	H209L	possibly damaging	Het
Eml5	G	T	12: 98,852,704 (GRCm38)		probably null	Het
Evpl	T	C	11: 116,223,660 (GRCm38)	E1068G	possibly damaging	Het
Ezh2	A	G	6: 47,576,660 (GRCm38)	M41T	probably damaging	Het
Fmo3	A	G	1: 162,968,725 (GRCm38)	S93P	possibly damaging	Het
Gdi2	A	G	13: 3,560,018 (GRCm38)	Y213C	possibly damaging	Het
Gpr15	A	T	16: 58,718,558 (GRCm38)	I56K	probably benign	Het
Gria2	G	T	3: 80,691,338 (GRCm38)	S796R	probably benign	Het
Hectd4	T	A	5: 121,291,191 (GRCm38)		probably benign	Het
Hgfac	T	C	5: 35,042,850 (GRCm38)		probably benign	Het
Hspg2	C	T	4: 137,520,156 (GRCm38)	R1200W	probably damaging	Het
Ift52	G	A	2: 163,025,355 (GRCm38)	D78N	possibly damaging	Het
Iqca1	C	A	1: 90,081,416 (GRCm38)	W415L	probably damaging	Het
Ism1	A	T	2: 139,746,043 (GRCm38)	K236N	probably damaging	Het
Itgb6	C	T	2: 60,672,644 (GRCm38)	W43*	probably null	Het
Kcnk18	T	A	19: 59,235,341 (GRCm38)	I306N	probably damaging	Het
Kctd8	T	C	5: 69,340,560 (GRCm38)	K248E	probably damaging	Het
Layn	T	A	9: 51,059,533 (GRCm38)	I237F	probably benign	Het
Lce3f	C	T	3: 92,992,941 (GRCm38)	P23L	unknown	Het
Lct	A	G	1: 128,300,301 (GRCm38)	F1152L	probably damaging	Het
Mettl25b	A	G	3: 87,923,817 (GRCm38)	S404P	probably damaging	Het
Mxra8	T	C	4: 155,843,074 (GRCm38)	I413T	probably damaging	Het
Nedd9	A	G	13: 41,317,962 (GRCm38)	V187A	probably benign	Het
Net1	A	G	13: 3,887,642 (GRCm38)	L207P	probably damaging	Het
Neurod1	G	T	2: 79,454,437 (GRCm38)	P201T	probably benign	Het
Nlrp1b	A	G	11: 71,161,821 (GRCm38)	F927S	probably damaging	Het
Ntrk3	T	A	7: 78,356,041 (GRCm38)	H524L	possibly damaging	Het
Nup98	A	C	7: 102,134,937 (GRCm38)	S1063A	probably benign	Het
Or10ak11	T	A	4: 118,829,868 (GRCm38)	M191L	probably benign	Het
Or11h4b	G	T	14: 50,681,166 (GRCm38)	P156Q	possibly damaging	Het
Or56b1	G	A	7: 104,636,159 (GRCm38)	V162I	probably benign	Het
Or5b97	C	A	19: 12,901,235 (GRCm38)	V182F	probably benign	Het
Or5m9b	G	A	2: 86,074,760 (GRCm38)	V7I	possibly damaging	Het
Or6d15	T	C	6: 116,582,964 (GRCm38)		probably benign	Het
Pdzd2	G	A	15: 12,592,460 (GRCm38)	R33W	probably damaging	Het
Phf21a	G	A	2: 92,330,515 (GRCm38)	V243I	probably damaging	Het
Ppp1r12b	A	G	1: 134,893,348 (GRCm38)		probably null	Het
Rexo5	T	C	7: 119,845,411 (GRCm38)	V703A	probably benign	Het
Rgs4	A	G	1: 169,745,278 (GRCm38)	S30P	probably benign	Het
Rxra	C	A	2: 27,756,244 (GRCm38)	D340E	probably damaging	Het
Samhd1	A	G	2: 157,107,547 (GRCm38)	V473A	probably benign	Het
Scn3a	T	A	2: 65,472,342 (GRCm38)	K1253N	probably damaging	Het
Scn7a	T	C	2: 66,680,955 (GRCm38)	N1207S	probably benign	Het
Sec24d	T	A	3: 123,336,517 (GRCm38)	I443N	probably damaging	Het
Sema4g	T	A	19: 44,999,242 (GRCm38)		probably null	Het
Sema5b	A	G	16: 35,660,324 (GRCm38)	K787R	probably benign	Het
Serpinc1	A	G	1: 160,989,647 (GRCm38)	N104D	probably benign	Het
Sfn	T	C	4: 133,601,231 (GRCm38)	H180R	probably damaging	Het
Slc22a6	G	T	19: 8,619,107 (GRCm38)		probably null	Het
Slc9a8	T	C	2: 167,457,358 (GRCm38)	S217P	probably benign	Het
Smc6	A	G	12: 11,309,269 (GRCm38)	N965D	probably benign	Het
Sod2	T	A	17: 13,015,032 (GRCm38)	I177N	probably damaging	Het
Sp4	A	T	12: 118,299,600 (GRCm38)	I237K	probably damaging	Het
Sulf2	T	C	2: 166,079,612 (GRCm38)	K697R	probably benign	Het
Svs4	A	T	2: 164,277,085 (GRCm38)	D110E	unknown	Het
Tas1r1	T	A	4: 152,038,218 (GRCm38)	R57*	probably null	Het
Tlr5	T	C	1: 182,973,722 (GRCm38)	I197T	probably damaging	Het
Tmc3	T	C	7: 83,612,532 (GRCm38)	V606A	probably benign	Het
Tmem241	T	A	18: 12,118,412 (GRCm38)	L37F	probably damaging	Het
Tmem59l	A	T	8: 70,486,253 (GRCm38)	N90K	probably damaging	Het
Tram1	A	C	1: 13,576,456 (GRCm38)		probably benign	Het
Trim15	T	A	17: 36,865,169 (GRCm38)	H162L	probably benign	Het
Ttc28	T	A	5: 111,276,811 (GRCm38)	Y1501N	probably benign	Het
Ttc34	T	C	4: 154,862,214 (GRCm38)	V857A	probably benign	Het
Tulp4	A	G	17: 6,139,046 (GRCm38)	T48A	probably damaging	Het
Usp9y	T	C	Y: 1,368,089 (GRCm38)	E939G	probably damaging	Het
Utp20	A	G	10: 88,770,808 (GRCm38)	I1634T	probably benign	Het
Vmn2r72	T	C	7: 85,738,170 (GRCm38)	M729V	probably benign	Het
Vps13c	C	T	9: 67,881,447 (GRCm38)	T334M	probably damaging	Het
Wnk4	C	G	11: 101,276,340 (GRCm38)		probably benign	Het
Xpo1	A	G	11: 23,271,193 (GRCm38)	N97D	probably benign	Het
Xpo4	A	T	14: 57,603,672 (GRCm38)	F518Y	probably benign	Het
Zfp668	C	T	7: 127,866,606 (GRCm38)	V469I	possibly damaging	Het
Zfp85	C	A	13: 67,749,704 (GRCm38)	C83F	probably damaging	Het

Other mutations in Ank2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01298:Ank2	APN	3	126,959,720 (GRCm38)	missense	possibly damaging	0.80
IGL01652:Ank2	APN	3	126,933,041 (GRCm38)	missense	probably benign	0.00
IGL01969:Ank2	APN	3	126,953,223 (GRCm38)	missense	possibly damaging	0.47
IGL02122:Ank2	APN	3	126,937,874 (GRCm38)	splice site	probably benign
IGL02537:Ank2	APN	3	126,955,916 (GRCm38)	missense	probably damaging	1.00
IGL02858:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL02981:Ank2	APN	3	126,934,562 (GRCm38)	missense	possibly damaging	0.58
IGL02981:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03024:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03074:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03111:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03129:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03174:Ank2	APN	3	126,940,095 (GRCm38)	missense	probably damaging	0.98
IGL03177:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03185:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03188:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03242:Ank2	APN	3	126,928,805 (GRCm38)	missense	possibly damaging	0.90
IGL03244:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03248:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03285:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03304:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03358:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03380:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03389:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03400:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
IGL03409:Ank2	APN	3	126,955,870 (GRCm38)	missense	probably damaging	1.00
ballast	UTSW	3	126,943,133 (GRCm38)	missense	unknown
Chain	UTSW	3	126,946,938 (GRCm38)	intron	probably benign
Deadman	UTSW	3	126,929,822 (GRCm38)	missense	probably benign	0.19
drag	UTSW	3	127,003,982 (GRCm38)	missense	probably damaging	1.00
mooring	UTSW	3	126,934,577 (GRCm38)	missense	possibly damaging	0.73
Treasure	UTSW	3	126,946,749 (GRCm38)	missense	unknown
Windlass	UTSW	3	126,946,149 (GRCm38)	missense	probably benign
R0033:Ank2	UTSW	3	127,104,748 (GRCm38)	splice site	probably benign
R0042:Ank2	UTSW	3	126,936,631 (GRCm38)	missense	probably damaging	0.99
R0042:Ank2	UTSW	3	126,936,631 (GRCm38)	missense	probably damaging	0.99
R0079:Ank2	UTSW	3	126,934,615 (GRCm38)	missense	probably benign	0.01
R0423:Ank2	UTSW	3	126,929,860 (GRCm38)	nonsense	probably null
R0699:Ank2	UTSW	3	126,929,829 (GRCm38)	missense	probably benign	0.00
R0724:Ank2	UTSW	3	126,962,337 (GRCm38)	missense	probably damaging	1.00
R0990:Ank2	UTSW	3	126,934,666 (GRCm38)	missense	possibly damaging	0.64
R1450:Ank2	UTSW	3	126,957,302 (GRCm38)	missense	possibly damaging	0.94
R1500:Ank2	UTSW	3	126,932,982 (GRCm38)	missense	probably benign
R1702:Ank2	UTSW	3	126,955,899 (GRCm38)	missense	probably benign	0.00
R1703:Ank2	UTSW	3	126,929,766 (GRCm38)	missense	probably damaging	1.00
R1710:Ank2	UTSW	3	126,933,060 (GRCm38)	nonsense	probably null
R1743:Ank2	UTSW	3	126,928,675 (GRCm38)	missense	probably damaging	0.99
R1852:Ank2	UTSW	3	126,997,851 (GRCm38)	critical splice donor site	probably null
R2198:Ank2	UTSW	3	126,934,577 (GRCm38)	missense	possibly damaging	0.73
R2892:Ank2	UTSW	3	127,248,243 (GRCm38)	splice site	probably null
R2893:Ank2	UTSW	3	127,248,243 (GRCm38)	splice site	probably null
R2894:Ank2	UTSW	3	127,248,243 (GRCm38)	splice site	probably null
R3148:Ank2	UTSW	3	126,933,075 (GRCm38)	missense	probably benign	0.00
R3776:Ank2	UTSW	3	126,942,262 (GRCm38)	intron	probably benign
R3784:Ank2	UTSW	3	126,953,193 (GRCm38)	missense	probably damaging	1.00
R3856:Ank2	UTSW	3	126,929,844 (GRCm38)	missense	probably benign	0.00
R3906:Ank2	UTSW	3	127,016,898 (GRCm38)	missense	probably damaging	1.00
R3907:Ank2	UTSW	3	127,016,898 (GRCm38)	missense	probably damaging	1.00
R3953:Ank2	UTSW	3	126,988,160 (GRCm38)	missense	probably damaging	1.00
R3963:Ank2	UTSW	3	126,934,596 (GRCm38)	missense	probably benign
R4367:Ank2	UTSW	3	126,946,149 (GRCm38)	missense	probably benign
R4414:Ank2	UTSW	3	127,225,762 (GRCm38)	critical splice donor site	probably null
R4432:Ank2	UTSW	3	126,947,806 (GRCm38)	intron	probably benign
R4433:Ank2	UTSW	3	126,947,806 (GRCm38)	intron	probably benign
R4579:Ank2	UTSW	3	126,958,963 (GRCm38)	missense	probably damaging	1.00
R4597:Ank2	UTSW	3	126,988,151 (GRCm38)	missense	probably damaging	1.00
R4603:Ank2	UTSW	3	127,032,016 (GRCm38)	missense	probably benign	0.00
R4729:Ank2	UTSW	3	126,976,896 (GRCm38)	nonsense	probably null
R4815:Ank2	UTSW	3	126,936,761 (GRCm38)	missense	probably benign
R4826:Ank2	UTSW	3	126,956,001 (GRCm38)	missense	probably benign	0.35
R4871:Ank2	UTSW	3	126,959,795 (GRCm38)	missense	probably damaging	1.00
R4880:Ank2	UTSW	3	127,046,826 (GRCm38)	splice site	probably null
R4915:Ank2	UTSW	3	126,942,671 (GRCm38)	intron	probably benign
R4935:Ank2	UTSW	3	126,956,064 (GRCm38)	missense	probably damaging	1.00
R4936:Ank2	UTSW	3	126,955,039 (GRCm38)	missense	possibly damaging	0.94
R4937:Ank2	UTSW	3	126,962,401 (GRCm38)	missense	probably damaging	1.00
R4946:Ank2	UTSW	3	126,941,940 (GRCm38)	intron	probably benign
R4963:Ank2	UTSW	3	127,032,096 (GRCm38)	missense	probably benign	0.01
R4989:Ank2	UTSW	3	126,963,445 (GRCm38)	missense	possibly damaging	0.94
R5023:Ank2	UTSW	3	126,941,871 (GRCm38)	intron	probably benign
R5060:Ank2	UTSW	3	126,945,921 (GRCm38)	intron	probably benign
R5078:Ank2	UTSW	3	126,942,353 (GRCm38)	intron	probably benign
R5086:Ank2	UTSW	3	126,947,348 (GRCm38)	intron	probably benign
R5134:Ank2	UTSW	3	126,963,445 (GRCm38)	missense	possibly damaging	0.94
R5148:Ank2	UTSW	3	127,025,636 (GRCm38)	splice site	probably null
R5175:Ank2	UTSW	3	127,004,024 (GRCm38)	missense	probably damaging	1.00
R5275:Ank2	UTSW	3	127,032,183 (GRCm38)	missense	probably damaging	1.00
R5295:Ank2	UTSW	3	127,032,183 (GRCm38)	missense	probably damaging	1.00
R5303:Ank2	UTSW	3	126,945,804 (GRCm38)	intron	probably benign
R5309:Ank2	UTSW	3	126,959,768 (GRCm38)	missense	probably damaging	0.99
R5312:Ank2	UTSW	3	126,959,768 (GRCm38)	missense	probably damaging	0.99
R5352:Ank2	UTSW	3	127,498,991 (GRCm38)	utr 5 prime	probably benign
R5355:Ank2	UTSW	3	126,944,049 (GRCm38)	intron	probably benign
R5386:Ank2	UTSW	3	126,981,933 (GRCm38)	missense	probably benign	0.01
R5396:Ank2	UTSW	3	126,953,226 (GRCm38)	missense	probably damaging	1.00
R5518:Ank2	UTSW	3	126,959,699 (GRCm38)	missense	probably damaging	0.98
R5534:Ank2	UTSW	3	126,947,298 (GRCm38)	intron	probably benign
R5554:Ank2	UTSW	3	126,998,973 (GRCm38)	missense	possibly damaging	0.78
R5582:Ank2	UTSW	3	126,946,305 (GRCm38)	intron	probably benign
R5747:Ank2	UTSW	3	126,941,751 (GRCm38)	intron	probably benign
R5794:Ank2	UTSW	3	126,930,020 (GRCm38)	missense	probably benign	0.00
R5831:Ank2	UTSW	3	127,339,159 (GRCm38)	start gained	probably benign
R5925:Ank2	UTSW	3	126,932,963 (GRCm38)	missense	probably benign	0.18
R5954:Ank2	UTSW	3	126,997,861 (GRCm38)	missense	probably benign	0.34
R5956:Ank2	UTSW	3	126,942,688 (GRCm38)	intron	probably benign
R5986:Ank2	UTSW	3	127,012,686 (GRCm38)	missense	possibly damaging	0.94
R5992:Ank2	UTSW	3	126,959,651 (GRCm38)	critical splice donor site	probably null
R6020:Ank2	UTSW	3	126,946,821 (GRCm38)	intron	probably benign
R6027:Ank2	UTSW	3	126,997,879 (GRCm38)	missense	possibly damaging	0.92
R6049:Ank2	UTSW	3	126,943,020 (GRCm38)	missense	possibly damaging	0.95
R6060:Ank2	UTSW	3	126,955,952 (GRCm38)	missense	probably damaging	1.00
R6114:Ank2	UTSW	3	127,011,051 (GRCm38)	missense	probably damaging	1.00
R6124:Ank2	UTSW	3	127,248,151 (GRCm38)	missense	probably benign	0.31
R6156:Ank2	UTSW	3	126,944,237 (GRCm38)	missense	probably damaging	1.00
R6173:Ank2	UTSW	3	127,052,746 (GRCm38)	missense	probably damaging	1.00
R6176:Ank2	UTSW	3	126,945,471 (GRCm38)	missense	probably benign	0.05
R6184:Ank2	UTSW	3	126,962,398 (GRCm38)	missense	probably damaging	1.00
R6199:Ank2	UTSW	3	127,004,006 (GRCm38)	missense	probably damaging	1.00
R6241:Ank2	UTSW	3	127,052,748 (GRCm38)	missense	probably damaging	1.00
R6254:Ank2	UTSW	3	126,941,804 (GRCm38)	intron	probably benign
R6259:Ank2	UTSW	3	127,016,986 (GRCm38)	missense	probably benign	0.28
R6260:Ank2	UTSW	3	126,943,557 (GRCm38)	missense	probably benign
R6321:Ank2	UTSW	3	126,946,938 (GRCm38)	intron	probably benign
R6393:Ank2	UTSW	3	126,929,757 (GRCm38)	missense	probably damaging	1.00
R6406:Ank2	UTSW	3	127,032,225 (GRCm38)	missense	probably damaging	1.00
R6544:Ank2	UTSW	3	126,933,222 (GRCm38)	missense	probably damaging	0.99
R6583:Ank2	UTSW	3	127,016,964 (GRCm38)	missense	probably damaging	1.00
R6739:Ank2	UTSW	3	127,079,994 (GRCm38)	missense	probably damaging	1.00
R6754:Ank2	UTSW	3	127,096,839 (GRCm38)	intron	probably benign
R6786:Ank2	UTSW	3	126,958,932 (GRCm38)	missense	probably damaging	0.99
R6798:Ank2	UTSW	3	126,944,264 (GRCm38)	intron	probably benign
R6882:Ank2	UTSW	3	126,945,757 (GRCm38)	intron	probably benign
R6940:Ank2	UTSW	3	126,941,972 (GRCm38)	intron	probably benign
R6949:Ank2	UTSW	3	127,010,884 (GRCm38)	missense	probably benign	0.00
R7001:Ank2	UTSW	3	127,077,581 (GRCm38)	missense	probably damaging	1.00
R7033:Ank2	UTSW	3	126,944,850 (GRCm38)	nonsense	probably null
R7036:Ank2	UTSW	3	126,946,392 (GRCm38)	intron	probably benign
R7045:Ank2	UTSW	3	127,012,744 (GRCm38)	missense	probably damaging	1.00
R7048:Ank2	UTSW	3	127,025,618 (GRCm38)	missense	probably benign	0.03
R7054:Ank2	UTSW	3	126,943,303 (GRCm38)	intron	probably benign
R7069:Ank2	UTSW	3	126,946,298 (GRCm38)	intron	probably benign
R7091:Ank2	UTSW	3	127,023,351 (GRCm38)	missense	probably damaging	0.98
R7107:Ank2	UTSW	3	127,003,982 (GRCm38)	missense	probably damaging	1.00
R7175:Ank2	UTSW	3	126,946,941 (GRCm38)	missense	unknown
R7191:Ank2	UTSW	3	126,946,392 (GRCm38)	missense	unknown
R7272:Ank2	UTSW	3	126,943,133 (GRCm38)	missense	unknown
R7381:Ank2	UTSW	3	126,936,628 (GRCm38)	missense	possibly damaging	0.46
R7394:Ank2	UTSW	3	126,936,653 (GRCm38)	missense	possibly damaging	0.77
R7462:Ank2	UTSW	3	126,943,034 (GRCm38)	missense	unknown
R7490:Ank2	UTSW	3	126,958,889 (GRCm38)	missense	probably damaging	0.99
R7514:Ank2	UTSW	3	127,025,603 (GRCm38)	missense	probably benign	0.06
R7534:Ank2	UTSW	3	126,934,333 (GRCm38)	splice site	probably null
R7540:Ank2	UTSW	3	126,988,159 (GRCm38)	missense	possibly damaging	0.94
R7547:Ank2	UTSW	3	126,945,203 (GRCm38)	missense	unknown
R7579:Ank2	UTSW	3	126,946,398 (GRCm38)	missense	unknown
R7584:Ank2	UTSW	3	126,946,128 (GRCm38)	nonsense	probably null
R7625:Ank2	UTSW	3	127,052,800 (GRCm38)	missense	probably damaging	1.00
R7698:Ank2	UTSW	3	127,032,211 (GRCm38)	missense	probably benign	0.35
R7716:Ank2	UTSW	3	126,943,166 (GRCm38)	missense	unknown
R7718:Ank2	UTSW	3	126,965,013 (GRCm38)	missense	possibly damaging	0.88
R7722:Ank2	UTSW	3	127,029,302 (GRCm38)	missense	probably benign	0.01
R7738:Ank2	UTSW	3	126,947,622 (GRCm38)	missense
R7977:Ank2	UTSW	3	126,945,707 (GRCm38)	missense	unknown
R7987:Ank2	UTSW	3	126,945,707 (GRCm38)	missense	unknown
R8007:Ank2	UTSW	3	126,936,447 (GRCm38)	intron	probably benign
R8150:Ank2	UTSW	3	126,947,513 (GRCm38)	missense
R8161:Ank2	UTSW	3	127,032,129 (GRCm38)	missense
R8196:Ank2	UTSW	3	126,929,883 (GRCm38)	missense	probably damaging	0.99
R8248:Ank2	UTSW	3	126,937,785 (GRCm38)	missense	possibly damaging	0.78
R8255:Ank2	UTSW	3	126,946,749 (GRCm38)	missense	unknown
R8279:Ank2	UTSW	3	126,933,171 (GRCm38)	missense	probably benign	0.04
R8300:Ank2	UTSW	3	127,010,906 (GRCm38)	missense
R8716:Ank2	UTSW	3	126,942,839 (GRCm38)	nonsense	probably null
R8724:Ank2	UTSW	3	126,943,756 (GRCm38)	missense	unknown
R8765:Ank2	UTSW	3	127,057,082 (GRCm38)	missense	possibly damaging	0.94
R8779:Ank2	UTSW	3	126,965,102 (GRCm38)	missense	probably damaging	0.99
R8783:Ank2	UTSW	3	127,052,806 (GRCm38)	missense	probably damaging	1.00
R8785:Ank2	UTSW	3	126,997,921 (GRCm38)	missense	probably damaging	1.00
R8826:Ank2	UTSW	3	126,947,302 (GRCm38)	missense	unknown
R8872:Ank2	UTSW	3	126,997,876 (GRCm38)	missense	possibly damaging	0.88
R8903:Ank2	UTSW	3	127,046,782 (GRCm38)	missense	probably damaging	1.00
R8906:Ank2	UTSW	3	126,933,071 (GRCm38)	missense	probably benign	0.00
R8918:Ank2	UTSW	3	126,943,731 (GRCm38)	missense	unknown
R8947:Ank2	UTSW	3	126,942,747 (GRCm38)	intron	probably benign
R8977:Ank2	UTSW	3	126,944,926 (GRCm38)	missense	unknown
R8990:Ank2	UTSW	3	127,048,180 (GRCm38)	critical splice donor site	probably null
R8994:Ank2	UTSW	3	126,929,822 (GRCm38)	missense	probably benign	0.19
R9009:Ank2	UTSW	3	126,934,376 (GRCm38)	unclassified	probably benign
R9123:Ank2	UTSW	3	126,940,095 (GRCm38)	missense	probably damaging	1.00
R9125:Ank2	UTSW	3	126,940,095 (GRCm38)	missense	probably damaging	1.00
R9130:Ank2	UTSW	3	127,016,916 (GRCm38)	missense
R9175:Ank2	UTSW	3	126,928,753 (GRCm38)	missense	possibly damaging	0.52
R9220:Ank2	UTSW	3	126,943,437 (GRCm38)	missense	unknown
R9225:Ank2	UTSW	3	126,942,462 (GRCm38)	missense	unknown
R9286:Ank2	UTSW	3	127,052,732 (GRCm38)	missense	probably damaging	0.99
R9325:Ank2	UTSW	3	126,981,855 (GRCm38)	missense	probably damaging	0.98
R9367:Ank2	UTSW	3	126,945,029 (GRCm38)	missense	unknown
R9385:Ank2	UTSW	3	126,959,717 (GRCm38)	missense	probably benign	0.00
R9391:Ank2	UTSW	3	126,937,745 (GRCm38)	missense	probably damaging	0.99
R9422:Ank2	UTSW	3	127,096,856 (GRCm38)	missense	unknown
R9536:Ank2	UTSW	3	126,942,382 (GRCm38)	missense	unknown
R9647:Ank2	UTSW	3	126,998,974 (GRCm38)	missense	possibly damaging	0.93
R9650:Ank2	UTSW	3	126,942,180 (GRCm38)	missense	unknown
R9666:Ank2	UTSW	3	126,933,189 (GRCm38)	nonsense	probably null
R9686:Ank2	UTSW	3	126,946,901 (GRCm38)	missense	unknown
R9730:Ank2	UTSW	3	127,225,844 (GRCm38)	missense
R9738:Ank2	UTSW	3	126,943,472 (GRCm38)	missense	unknown
R9743:Ank2	UTSW	3	126,940,145 (GRCm38)	missense	possibly damaging	0.81
R9747:Ank2	UTSW	3	126,959,018 (GRCm38)	missense	probably damaging	1.00
R9800:Ank2	UTSW	3	126,946,500 (GRCm38)	missense	unknown
R9803:Ank2	UTSW	3	126,959,077 (GRCm38)	missense	possibly damaging	0.64
RF020:Ank2	UTSW	3	126,945,476 (GRCm38)	missense	unknown
Z1088:Ank2	UTSW	3	127,029,509 (GRCm38)	missense	possibly damaging	0.45
Z1177:Ank2	UTSW	3	126,944,357 (GRCm38)	missense	unknown
Z1187:Ank2	UTSW	3	126,955,952 (GRCm38)	missense	probably damaging	1.00
Z1190:Ank2	UTSW	3	126,955,952 (GRCm38)	missense	probably damaging	1.00
Z1192:Ank2	UTSW	3	126,955,952 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGCCTCACACAACACTGTC -3'
(R):5'- TCTGTGCTGCCAAGGAGAAG -3'

Sequencing Primer
(F):5'- ACACAACACTGTCACAATTATGTATC -3'
(R):5'- CTGCCAAGGAGAAGAAGGAGC -3'

Posted On

2014-06-23