Mutagenetix > Incidental Mutation

Incidental Mutation 'R3788:Flnc'

272371

Institutional Source

Beutler Lab

Gene Symbol

Flnc

Ensembl Gene

ENSMUSG00000068699

Gene Name

filamin C, gamma

Synonyms

1110055E19Rik, actin binding protein 280, Fln2

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R3788 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

29433256-29461883 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 29454057 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 1820 (F1820L)

Ref Sequence

ENSEMBL: ENSMUSP00000099139 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065090] [ENSMUST00000101617]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000065090
AA Change: F1853L

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000064163
Gene: ENSMUSG00000068699
AA Change: F1853L

Domain	Start	End	E-Value	Type
CH	39	141	1.17e-25	SMART
CH	162	258	2.09e-22	SMART
IG_FLMN	275	372	3.84e-38	SMART
IG_FLMN	375	472	3.73e-36	SMART
IG_FLMN	474	569	4.41e-38	SMART
IG_FLMN	571	662	3.61e-34	SMART
IG_FLMN	667	762	2.48e-41	SMART
IG_FLMN	764	865	6.7e-29	SMART
IG_FLMN	867	964	3.42e-35	SMART
IG_FLMN	966	1060	2.92e-29	SMART
IG_FLMN	1062	1153	3.69e-40	SMART
IG_FLMN	1155	1248	6.53e-41	SMART
IG_FLMN	1250	1348	7.23e-34	SMART
IG_FLMN	1350	1441	2.04e-42	SMART
IG_FLMN	1443	1537	1.75e-41	SMART
IG_FLMN	1539	1634	1.31e-40	SMART
IG_FLMN	1636	1738	1.05e-30	SMART
IG_FLMN	1777	1858	2.93e-11	SMART
IG_FLMN	1859	1950	2.55e-43	SMART
IG_FLMN	1951	2037	2.43e-17	SMART
IG_FLMN	2041	2132	1.52e-41	SMART
PDB:2E9I\|A	2133	2162	3e-7	PDB
IG_FLMN	2217	2310	2.93e-11	SMART
IG_FLMN	2314	2405	1.67e-38	SMART
IG_FLMN	2408	2500	2.56e-25	SMART
IG_FLMN	2505	2596	9.54e-34	SMART
low complexity region	2618	2628	N/A	INTRINSIC
IG_FLMN	2635	2737	2.11e-26	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000101617
AA Change: F1820L

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000099139
Gene: ENSMUSG00000068699
AA Change: F1820L

Domain	Start	End	E-Value	Type
CH	39	141	1.17e-25	SMART
CH	162	258	2.09e-22	SMART
IG_FLMN	275	372	3.84e-38	SMART
IG_FLMN	375	472	3.73e-36	SMART
IG_FLMN	474	569	4.41e-38	SMART
IG_FLMN	571	662	3.61e-34	SMART
IG_FLMN	667	762	2.48e-41	SMART
IG_FLMN	764	865	6.7e-29	SMART
IG_FLMN	867	964	3.42e-35	SMART
IG_FLMN	966	1060	2.92e-29	SMART
IG_FLMN	1062	1153	3.69e-40	SMART
IG_FLMN	1155	1248	6.53e-41	SMART
IG_FLMN	1250	1348	7.23e-34	SMART
IG_FLMN	1350	1441	2.04e-42	SMART
IG_FLMN	1443	1537	1.75e-41	SMART
IG_FLMN	1539	1634	1.31e-40	SMART
IG_FLMN	1636	1738	6.11e-32	SMART
IG_FLMN	1744	1825	2.93e-11	SMART
IG_FLMN	1826	1917	2.55e-43	SMART
IG_FLMN	1918	2004	2.43e-17	SMART
IG_FLMN	2008	2099	1.52e-41	SMART
PDB:2E9I\|A	2100	2129	3e-7	PDB
IG_FLMN	2184	2277	2.93e-11	SMART
IG_FLMN	2281	2372	1.67e-38	SMART
IG_FLMN	2375	2467	2.56e-25	SMART
IG_FLMN	2472	2563	9.54e-34	SMART
low complexity region	2585	2595	N/A	INTRINSIC
IG_FLMN	2602	2704	2.11e-26	SMART

Meta Mutation Damage Score

0.2948

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of three related filamin genes, specifically gamma filamin. These filamin proteins crosslink actin filaments into orthogonal networks in cortical cytoplasm and participate in the anchoring of membrane proteins for the actin cytoskeleton. Three functional domains exist in filamin: an N-terminal filamentous actin-binding domain, a C-terminal self-association domain, and a membrane glycoprotein-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a hypomorphic allele display neonatal lethality, respiratory failure, reduced skeletal muscle mass, and abnormal skeletal muscle fiber morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610507B11Rik	T	A	11: 78,288,297 (GRCm38)		probably null	Het
Abca4	T	A	3: 122,052,912 (GRCm38)	V26E	possibly damaging	Het
Abhd16a	A	G	17: 35,101,587 (GRCm38)	N411S	probably damaging	Het
Akap13	G	A	7: 75,702,153 (GRCm38)		probably null	Het
Aph1b	T	C	9: 66,794,066 (GRCm38)		probably benign	Het
Aspm	C	T	1: 139,463,203 (GRCm38)	T742I	probably damaging	Het
Bclaf3	A	G	X: 159,566,496 (GRCm38)	H619R	probably benign	Het
Cemip	A	T	7: 83,943,898 (GRCm38)	L1199H	probably damaging	Het
Chd2	G	A	7: 73,447,130 (GRCm38)		probably benign	Het
Clnk	A	G	5: 38,714,998 (GRCm38)	Y310H	probably damaging	Het
Crmp1	A	G	5: 37,284,140 (GRCm38)	D522G	probably damaging	Het
Cyth3	A	G	5: 143,636,543 (GRCm38)		probably benign	Het
Dcbld1	T	A	10: 52,319,658 (GRCm38)	Y392N	probably damaging	Het
Fam60a	A	G	6: 148,926,119 (GRCm38)	S134P	possibly damaging	Het
Galnt18	G	A	7: 111,520,115 (GRCm38)	R385*	probably null	Het
Gpatch3	C	A	4: 133,575,168 (GRCm38)	R137S	possibly damaging	Het
Gpc6	C	T	14: 117,624,466 (GRCm38)	P265S	probably damaging	Het
Harbi1	T	A	2: 91,720,607 (GRCm38)	D308E	probably benign	Het
Hdhd2	G	A	18: 76,955,187 (GRCm38)		probably null	Het
Hk1	T	C	10: 62,275,688 (GRCm38)	K737E	possibly damaging	Het
Hnrnpr	G	A	4: 136,336,313 (GRCm38)	V345M	probably damaging	Het
Kalrn	T	C	16: 34,220,240 (GRCm38)	H944R	probably damaging	Het
Kdm2a	A	T	19: 4,351,805 (GRCm38)	C207S	probably damaging	Het
Kirrel	C	T	3: 87,089,151 (GRCm38)	M380I	probably null	Het
Krt75	C	T	15: 101,573,521 (GRCm38)	G104D	possibly damaging	Het
Lnpk	A	T	2: 74,522,263 (GRCm38)	S358R	probably benign	Het
Map2	A	G	1: 66,416,863 (GRCm38)	T1512A	probably damaging	Het
March10	T	A	11: 105,397,079 (GRCm38)	L132F	probably damaging	Het
Mfrp	G	A	9: 44,105,457 (GRCm38)	W65*	probably null	Het
Mgat5	A	G	1: 127,366,443 (GRCm38)	D174G	probably benign	Het
Miga2	T	A	2: 30,371,225 (GRCm38)	Y177*	probably null	Het
Mroh3	T	C	1: 136,185,475 (GRCm38)	D747G	probably damaging	Het
Muc5b	A	G	7: 141,863,834 (GRCm38)	T3506A	possibly damaging	Het
Myo7b	G	T	18: 31,974,112 (GRCm38)	P1277T	possibly damaging	Het
Naaa	C	T	5: 92,272,554 (GRCm38)		probably null	Het
Ndufs2	T	C	1: 171,235,320 (GRCm38)	D410G	possibly damaging	Het
Olfr506	T	A	7: 108,613,073 (GRCm38)	Y255*	probably null	Het
Olfr559	A	G	7: 102,723,487 (GRCm38)		probably null	Het
Olfr873	A	G	9: 20,300,370 (GRCm38)	I58V	probably benign	Het
Olfr955	A	G	9: 39,470,069 (GRCm38)	I219T	probably benign	Het
Osbp	A	T	19: 11,978,921 (GRCm38)	Y409F	probably benign	Het
Plxnb1	T	A	9: 109,109,287 (GRCm38)	V1303D	possibly damaging	Het
Prkcg	G	A	7: 3,313,747 (GRCm38)	D246N	probably damaging	Het
Ranbp17	GCCTGGATACTGACC	GCC	11: 33,219,203 (GRCm38)		probably benign	Het
Sbf1	G	A	15: 89,299,528 (GRCm38)	R1261*	probably null	Het
Scn4a	T	C	11: 106,344,274 (GRCm38)	N341S	probably damaging	Het
Sec61a2	C	A	2: 5,879,625 (GRCm38)		probably null	Het
Sgcd	T	A	11: 47,355,205 (GRCm38)	K57*	probably null	Het
Slc12a5	T	C	2: 164,993,775 (GRCm38)	L861P	probably damaging	Het
Slc6a16	A	G	7: 45,259,962 (GRCm38)	D184G	probably benign	Het
Snx7	A	G	3: 117,838,990 (GRCm38)		probably benign	Het
Sptbn2	A	G	19: 4,745,922 (GRCm38)	I1710V	probably damaging	Het
Sytl2	A	T	7: 90,376,081 (GRCm38)	I426F	probably benign	Het
Tdp1	A	G	12: 99,891,752 (GRCm38)		probably benign	Het
Tmem232	C	A	17: 65,382,633 (GRCm38)	D496Y	possibly damaging	Het
Tomm20l	C	T	12: 71,111,742 (GRCm38)	A58V	possibly damaging	Het
Ttc26	T	A	6: 38,403,524 (GRCm38)		probably null	Het
Ttn	A	G	2: 76,974,208 (GRCm38)	V240A	probably benign	Het
Ttn	T	C	2: 76,945,274 (GRCm38)	E1854G	unknown	Het
Vmn2r98	A	T	17: 19,080,625 (GRCm38)	T630S	probably benign	Het
Xrcc1	G	C	7: 24,566,908 (GRCm38)	A220P	probably benign	Het

Other mutations in Flnc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00952:Flnc	APN	6	29,459,547 (GRCm38)	nonsense	probably null
IGL01099:Flnc	APN	6	29,433,618 (GRCm38)	missense	probably damaging	0.99
IGL01656:Flnc	APN	6	29,443,508 (GRCm38)	splice site	probably benign
IGL01659:Flnc	APN	6	29,448,671 (GRCm38)	missense	probably damaging	0.98
IGL01780:Flnc	APN	6	29,438,493 (GRCm38)	nonsense	probably null
IGL01935:Flnc	APN	6	29,454,280 (GRCm38)	missense	probably damaging	1.00
IGL02039:Flnc	APN	6	29,450,719 (GRCm38)	missense	probably benign	0.05
IGL02119:Flnc	APN	6	29,447,512 (GRCm38)	missense	probably damaging	0.98
IGL02122:Flnc	APN	6	29,444,336 (GRCm38)	missense	possibly damaging	0.70
IGL02236:Flnc	APN	6	29,454,376 (GRCm38)	missense	probably damaging	1.00
IGL02350:Flnc	APN	6	29,438,493 (GRCm38)	nonsense	probably null
IGL02357:Flnc	APN	6	29,438,493 (GRCm38)	nonsense	probably null
IGL02428:Flnc	APN	6	29,451,485 (GRCm38)	missense	probably damaging	1.00
IGL02496:Flnc	APN	6	29,440,685 (GRCm38)	missense	probably damaging	0.98
IGL02516:Flnc	APN	6	29,450,841 (GRCm38)	missense	probably damaging	0.99
IGL02696:Flnc	APN	6	29,446,698 (GRCm38)	missense	probably damaging	0.98
IGL03165:Flnc	APN	6	29,449,378 (GRCm38)	missense	probably damaging	1.00
IGL03190:Flnc	APN	6	29,445,637 (GRCm38)	splice site	probably benign
I1329:Flnc	UTSW	6	29,451,415 (GRCm38)	missense	probably damaging	1.00
R0111:Flnc	UTSW	6	29,454,340 (GRCm38)	missense	probably damaging	0.99
R0665:Flnc	UTSW	6	29,455,531 (GRCm38)	missense	probably damaging	1.00
R0748:Flnc	UTSW	6	29,446,344 (GRCm38)	missense	probably damaging	0.99
R0960:Flnc	UTSW	6	29,441,512 (GRCm38)	missense	probably damaging	1.00
R1328:Flnc	UTSW	6	29,438,613 (GRCm38)	missense	probably damaging	1.00
R1502:Flnc	UTSW	6	29,438,694 (GRCm38)	missense	probably benign	0.45
R1544:Flnc	UTSW	6	29,444,080 (GRCm38)	missense	probably benign	0.00
R1565:Flnc	UTSW	6	29,455,171 (GRCm38)	missense	probably damaging	1.00
R1640:Flnc	UTSW	6	29,433,807 (GRCm38)	missense	possibly damaging	0.78
R1691:Flnc	UTSW	6	29,441,214 (GRCm38)	missense	probably benign	0.09
R1818:Flnc	UTSW	6	29,457,448 (GRCm38)	missense	probably damaging	1.00
R1826:Flnc	UTSW	6	29,455,185 (GRCm38)	missense	probably damaging	0.99
R1851:Flnc	UTSW	6	29,443,479 (GRCm38)	missense	probably damaging	1.00
R1898:Flnc	UTSW	6	29,438,666 (GRCm38)	nonsense	probably null
R1905:Flnc	UTSW	6	29,459,460 (GRCm38)	missense	probably damaging	1.00
R1985:Flnc	UTSW	6	29,444,416 (GRCm38)	splice site	probably benign
R2016:Flnc	UTSW	6	29,443,797 (GRCm38)	critical splice donor site	probably null
R2017:Flnc	UTSW	6	29,443,797 (GRCm38)	critical splice donor site	probably null
R2020:Flnc	UTSW	6	29,444,363 (GRCm38)	missense	probably damaging	0.97
R2104:Flnc	UTSW	6	29,450,735 (GRCm38)	critical splice donor site	probably null
R2132:Flnc	UTSW	6	29,443,676 (GRCm38)	missense	probably damaging	1.00
R2141:Flnc	UTSW	6	29,448,675 (GRCm38)	missense	probably damaging	1.00
R2197:Flnc	UTSW	6	29,459,135 (GRCm38)	missense	probably damaging	1.00
R2202:Flnc	UTSW	6	29,459,508 (GRCm38)	missense	probably damaging	1.00
R2203:Flnc	UTSW	6	29,459,508 (GRCm38)	missense	probably damaging	1.00
R2204:Flnc	UTSW	6	29,459,508 (GRCm38)	missense	probably damaging	1.00
R2205:Flnc	UTSW	6	29,459,508 (GRCm38)	missense	probably damaging	1.00
R2209:Flnc	UTSW	6	29,455,845 (GRCm38)	missense	possibly damaging	0.91
R2248:Flnc	UTSW	6	29,451,401 (GRCm38)	missense	probably damaging	0.99
R2258:Flnc	UTSW	6	29,438,666 (GRCm38)	nonsense	probably null
R2259:Flnc	UTSW	6	29,438,666 (GRCm38)	nonsense	probably null
R2280:Flnc	UTSW	6	29,438,666 (GRCm38)	nonsense	probably null
R2281:Flnc	UTSW	6	29,438,666 (GRCm38)	nonsense	probably null
R2873:Flnc	UTSW	6	29,447,543 (GRCm38)	missense	probably damaging	0.96
R2900:Flnc	UTSW	6	29,448,585 (GRCm38)	missense	probably damaging	0.98
R3799:Flnc	UTSW	6	29,443,739 (GRCm38)	missense	probably damaging	1.00
R3801:Flnc	UTSW	6	29,447,404 (GRCm38)	missense	probably damaging	0.98
R3851:Flnc	UTSW	6	29,453,719 (GRCm38)	missense	probably damaging	1.00
R3910:Flnc	UTSW	6	29,459,427 (GRCm38)	missense	probably damaging	1.00
R3982:Flnc	UTSW	6	29,442,941 (GRCm38)	missense	probably damaging	1.00
R3983:Flnc	UTSW	6	29,442,941 (GRCm38)	missense	probably damaging	1.00
R4023:Flnc	UTSW	6	29,451,635 (GRCm38)	missense	possibly damaging	0.95
R4676:Flnc	UTSW	6	29,445,154 (GRCm38)	splice site	probably null
R4694:Flnc	UTSW	6	29,443,448 (GRCm38)	missense	probably damaging	1.00
R4695:Flnc	UTSW	6	29,440,429 (GRCm38)	missense	probably damaging	0.99
R4735:Flnc	UTSW	6	29,455,813 (GRCm38)	missense	probably damaging	1.00
R4773:Flnc	UTSW	6	29,445,039 (GRCm38)	missense	possibly damaging	0.96
R4828:Flnc	UTSW	6	29,455,167 (GRCm38)	missense	probably damaging	1.00
R4856:Flnc	UTSW	6	29,447,890 (GRCm38)	missense	probably damaging	1.00
R4879:Flnc	UTSW	6	29,460,806 (GRCm38)	missense	probably damaging	0.99
R4899:Flnc	UTSW	6	29,446,843 (GRCm38)	missense	probably benign	0.17
R4906:Flnc	UTSW	6	29,447,525 (GRCm38)	missense	probably damaging	0.99
R5089:Flnc	UTSW	6	29,447,813 (GRCm38)	missense	probably damaging	0.96
R5173:Flnc	UTSW	6	29,455,538 (GRCm38)	missense	probably damaging	1.00
R5174:Flnc	UTSW	6	29,448,894 (GRCm38)	missense	possibly damaging	0.91
R5290:Flnc	UTSW	6	29,457,554 (GRCm38)	missense	probably damaging	1.00
R5338:Flnc	UTSW	6	29,444,064 (GRCm38)	missense	possibly damaging	0.47
R5352:Flnc	UTSW	6	29,449,318 (GRCm38)	missense	possibly damaging	0.85
R5397:Flnc	UTSW	6	29,441,161 (GRCm38)	missense	possibly damaging	0.87
R5431:Flnc	UTSW	6	29,456,384 (GRCm38)	missense	possibly damaging	0.74
R5481:Flnc	UTSW	6	29,441,217 (GRCm38)	missense	probably damaging	1.00
R5511:Flnc	UTSW	6	29,458,898 (GRCm38)	missense	probably damaging	1.00
R5539:Flnc	UTSW	6	29,446,230 (GRCm38)	missense	probably damaging	1.00
R5549:Flnc	UTSW	6	29,453,691 (GRCm38)	missense	probably damaging	1.00
R5567:Flnc	UTSW	6	29,444,045 (GRCm38)	nonsense	probably null
R5584:Flnc	UTSW	6	29,446,628 (GRCm38)	missense	probably damaging	0.98
R5689:Flnc	UTSW	6	29,441,592 (GRCm38)	missense	probably benign	0.03
R5753:Flnc	UTSW	6	29,433,489 (GRCm38)	missense	probably benign
R5786:Flnc	UTSW	6	29,459,537 (GRCm38)	nonsense	probably null
R5822:Flnc	UTSW	6	29,459,430 (GRCm38)	missense	probably damaging	0.98
R5823:Flnc	UTSW	6	29,461,202 (GRCm38)	missense	probably damaging	0.99
R5933:Flnc	UTSW	6	29,441,106 (GRCm38)	missense	probably damaging	0.99
R6043:Flnc	UTSW	6	29,446,608 (GRCm38)	missense	probably damaging	1.00
R6320:Flnc	UTSW	6	29,459,063 (GRCm38)	missense	probably damaging	1.00
R6337:Flnc	UTSW	6	29,454,319 (GRCm38)	missense	probably damaging	0.99
R6399:Flnc	UTSW	6	29,458,883 (GRCm38)	missense	probably damaging	1.00
R6423:Flnc	UTSW	6	29,445,156 (GRCm38)	splice site	probably null
R6540:Flnc	UTSW	6	29,446,377 (GRCm38)	missense	possibly damaging	0.96
R6547:Flnc	UTSW	6	29,448,608 (GRCm38)	missense	probably damaging	0.98
R6717:Flnc	UTSW	6	29,450,902 (GRCm38)	small deletion	probably benign
R6875:Flnc	UTSW	6	29,445,749 (GRCm38)	missense	probably damaging	1.00
R7193:Flnc	UTSW	6	29,450,871 (GRCm38)	missense	probably damaging	1.00
R7255:Flnc	UTSW	6	29,445,766 (GRCm38)	missense	probably damaging	1.00
R7303:Flnc	UTSW	6	29,460,850 (GRCm38)	missense	probably benign	0.31
R7413:Flnc	UTSW	6	29,452,259 (GRCm38)	missense	probably damaging	1.00
R7422:Flnc	UTSW	6	29,455,471 (GRCm38)	missense	probably damaging	1.00
R7559:Flnc	UTSW	6	29,459,010 (GRCm38)	missense	probably damaging	1.00
R7632:Flnc	UTSW	6	29,446,985 (GRCm38)	missense	probably damaging	0.98
R7651:Flnc	UTSW	6	29,444,050 (GRCm38)	missense	probably benign	0.08
R7679:Flnc	UTSW	6	29,456,790 (GRCm38)	missense	probably benign	0.00
R7697:Flnc	UTSW	6	29,456,517 (GRCm38)	missense	probably damaging	0.98
R7788:Flnc	UTSW	6	29,456,444 (GRCm38)	missense	possibly damaging	0.67
R7852:Flnc	UTSW	6	29,440,898 (GRCm38)	missense	probably damaging	1.00
R7870:Flnc	UTSW	6	29,454,307 (GRCm38)	missense	probably damaging	1.00
R7873:Flnc	UTSW	6	29,456,991 (GRCm38)	missense	possibly damaging	0.88
R7921:Flnc	UTSW	6	29,447,770 (GRCm38)	missense	possibly damaging	0.58
R7950:Flnc	UTSW	6	29,456,382 (GRCm38)	missense	possibly damaging	0.61
R7953:Flnc	UTSW	6	29,447,829 (GRCm38)	missense	probably damaging	0.99
R7970:Flnc	UTSW	6	29,447,526 (GRCm38)	missense	possibly damaging	0.96
R8071:Flnc	UTSW	6	29,457,446 (GRCm38)	missense	probably damaging	1.00
R8143:Flnc	UTSW	6	29,441,485 (GRCm38)	missense	probably benign	0.20
R8166:Flnc	UTSW	6	29,433,732 (GRCm38)	missense	probably damaging	0.99
R8167:Flnc	UTSW	6	29,455,922 (GRCm38)	missense	probably damaging	0.98
R8306:Flnc	UTSW	6	29,449,370 (GRCm38)	missense	probably benign	0.05
R8428:Flnc	UTSW	6	29,450,850 (GRCm38)	missense	probably benign	0.36
R8466:Flnc	UTSW	6	29,438,622 (GRCm38)	missense	probably damaging	0.98
R8671:Flnc	UTSW	6	29,443,502 (GRCm38)	critical splice donor site	probably null
R8885:Flnc	UTSW	6	29,455,411 (GRCm38)	missense	probably damaging	0.96
R8922:Flnc	UTSW	6	29,456,836 (GRCm38)	missense	probably damaging	0.99
R8923:Flnc	UTSW	6	29,452,237 (GRCm38)	missense	probably damaging	1.00
R8985:Flnc	UTSW	6	29,440,500 (GRCm38)	missense	probably benign	0.37
R9075:Flnc	UTSW	6	29,447,647 (GRCm38)	missense	probably damaging	0.96
R9098:Flnc	UTSW	6	29,455,519 (GRCm38)	nonsense	probably null
R9162:Flnc	UTSW	6	29,455,861 (GRCm38)	missense	probably damaging	1.00
R9199:Flnc	UTSW	6	29,441,491 (GRCm38)	missense	probably benign	0.31
R9204:Flnc	UTSW	6	29,452,354 (GRCm38)	missense	possibly damaging	0.93
R9273:Flnc	UTSW	6	29,447,816 (GRCm38)	missense	probably benign	0.08
R9411:Flnc	UTSW	6	29,441,485 (GRCm38)	missense	probably benign
R9412:Flnc	UTSW	6	29,441,485 (GRCm38)	missense	probably benign
R9413:Flnc	UTSW	6	29,441,485 (GRCm38)	missense	probably benign
R9451:Flnc	UTSW	6	29,445,463 (GRCm38)	missense	probably damaging	0.98
R9524:Flnc	UTSW	6	29,461,110 (GRCm38)	missense	probably damaging	1.00
R9575:Flnc	UTSW	6	29,454,400 (GRCm38)	missense	probably damaging	0.98
R9582:Flnc	UTSW	6	29,460,737 (GRCm38)	missense	probably damaging	0.99
R9595:Flnc	UTSW	6	29,433,721 (GRCm38)	missense	probably benign	0.05
R9664:Flnc	UTSW	6	29,457,215 (GRCm38)	missense	probably damaging	1.00
R9665:Flnc	UTSW	6	29,455,448 (GRCm38)	missense	probably damaging	1.00
R9686:Flnc	UTSW	6	29,456,435 (GRCm38)	missense	possibly damaging	0.84
Z1088:Flnc	UTSW	6	29,457,151 (GRCm38)	missense	probably damaging	1.00
Z1177:Flnc	UTSW	6	29,457,130 (GRCm38)	missense	probably damaging	1.00
Z1177:Flnc	UTSW	6	29,447,545 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGTAAGAACCTGCCATGTACAG -3'
(R):5'- GAGGTCAGAGGGTCTATCCTTG -3'

Sequencing Primer
(F):5'- TGCCATGTACAGGCACCATG -3'
(R):5'- CAGAGGGTCTATCCTTGTGCCG -3'

Posted On

2015-03-25