Mutagenetix > Incidental Mutations

Incidental Mutation 'R4870:Cdc14a'

376484

Institutional Source

Beutler Lab

Gene Symbol

Cdc14a

Ensembl Gene

ENSMUSG00000033502

Gene Name

CDC14 cell division cycle 14A

Synonyms

CDC14A2, Cdc14, CDC14a1, A830059A17Rik

MMRRC Submission

042480-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.225) question?

Stock #

R4870 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

116272553-116424032 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 116423460 bp

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 9 (I9T)

Ref Sequence

ENSEMBL: ENSMUSP00000102100 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090464] [ENSMUST00000106491] [ENSMUST00000197830]

Predicted Effect

probably benign
Transcript: ENSMUST00000090464
AA Change: I9T

PolyPhen 2 Score 0.298 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000087950
Gene: ENSMUSG00000033502
AA Change: I9T

Domain	Start	End	E-Value	Type
Pfam:DSPn	13	153	1.6e-66	PFAM
Pfam:Y_phosphatase	210	324	1.1e-7	PFAM
Pfam:DSPc	214	328	1.8e-14	PFAM
low complexity region	539	558	N/A	INTRINSIC
low complexity region	573	595	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106491
AA Change: I9T

PolyPhen 2 Score 0.298 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000102100
Gene: ENSMUSG00000033502
AA Change: I9T

Domain	Start	End	E-Value	Type
Pfam:DSPn	12	121	9.5e-44	PFAM
Pfam:Y_phosphatase	160	274	6.4e-8	PFAM
Pfam:DSPc	160	280	2.8e-14	PFAM
low complexity region	490	509	N/A	INTRINSIC
low complexity region	524	546	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127704

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136994

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148857

Predicted Effect

probably benign
Transcript: ENSMUST00000197830

Meta Mutation Damage Score

0.0765

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.9%
20x: 94.1%

Validation Efficiency

100% (77/77)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931406P16Rik	C	A	7: 34,284,887	V104L	possibly damaging	Het
Abcb11	A	T	2: 69,239,196	I1285N	probably damaging	Het
Abcc8	G	A	7: 46,107,259	R721*	probably null	Het
Alox8	T	C	11: 69,186,568	Y423C	probably damaging	Het
Ankle2	T	A	5: 110,251,478		probably null	Het
Ceacam18	T	C	7: 43,641,904	C257R	probably damaging	Het
Cilp	T	C	9: 65,279,698	V1025A	probably damaging	Het
Clcn7	G	T	17: 25,153,565		probably benign	Het
Csnk1d	A	T	11: 120,983,188		probably benign	Het
Cyp11b2	A	G	15: 74,853,146	S285P	probably benign	Het
Dip2b	T	A	15: 100,195,784		probably null	Het
Dmrt1	T	A	19: 25,505,855	M1K	probably null	Het
Dnajc14	T	C	10: 128,817,350	V684A	probably benign	Het
Dnmt3b	A	G	2: 153,670,364	Q335R	probably benign	Het
Exoc3l2	T	A	7: 19,495,192	C772S	unknown	Het
F2rl1	A	T	13: 95,513,984	F130Y	probably damaging	Het
Galk2	C	G	2: 125,929,637	S194*	probably null	Het
Gm7102	C	T	19: 61,175,926	G24R	unknown	Het
Gpbp1l1	A	G	4: 116,573,517	T62A	probably benign	Het
H2-Q10	A	T	17: 35,470,460	D53V	probably damaging	Het
H2-T22	T	C	17: 36,039,032	K356R	probably benign	Het
Insrr	G	A	3: 87,811,604	V956M	probably damaging	Het
Ints7	A	G	1: 191,596,331	T239A	probably damaging	Het
Isl1	A	G	13: 116,308,270		probably benign	Het
Kcng2	A	G	18: 80,322,868	C90R	probably benign	Het
Kif3c	C	T	12: 3,401,735	P171S	probably damaging	Het
Knl1	A	G	2: 119,081,513	T1704A	probably benign	Het
Lats1	A	G	10: 7,705,785	Y778C	probably damaging	Het
Limd2	T	C	11: 106,159,389	M1V	probably null	Het
Mcm10	A	T	2: 5,004,159	I333N	probably damaging	Het
Mipep	T	A	14: 60,802,880	L283*	probably null	Het
Mixl1	A	G	1: 180,694,672	S215P	probably benign	Het
Mmp21	A	G	7: 133,678,677	L188P	probably damaging	Het
Mob1a	T	C	6: 83,340,239	S213P	probably benign	Het
Ndufaf6	T	C	4: 11,060,917	T220A	probably benign	Het
Nr4a3	A	T	4: 48,051,651	Y135F	possibly damaging	Het
Ntn1	CCTTCTTCT	CCTTCT	11: 68,213,026		probably benign	Het
Obscn	A	T	11: 59,136,206	L57Q	probably damaging	Het
Olfr1161	T	C	2: 88,025,460	L246P	probably damaging	Het
Olfr603	T	A	7: 103,383,633	D123V	probably damaging	Het
Pirb	T	A	7: 3,712,662	M839L	probably benign	Het
Plcl2	A	G	17: 50,607,226	E421G	possibly damaging	Het
Ppp1r12b	T	C	1: 134,949,033	N99S	probably benign	Het
Ptpro	A	G	6: 137,377,132	K169E	probably damaging	Het
Rita1	T	A	5: 120,611,383	K88N	probably damaging	Het
Rptn	A	T	3: 93,396,469	K370*	probably null	Het
Simc1	A	G	13: 54,539,763	D115G	probably null	Het
Stab1	T	A	14: 31,142,043	N136I	probably benign	Het
Syt4	T	A	18: 31,447,356		probably benign	Het
Sytl2	A	T	7: 90,388,898	N522I	probably damaging	Het
Tax1bp1	A	G	6: 52,729,493		probably benign	Het
Tenm2	A	T	11: 36,078,569	D847E	probably damaging	Het
Th	G	T	7: 142,894,097	D321E	probably benign	Het
Tmem44	A	T	16: 30,540,773	L46Q	probably damaging	Het
Trp53bp1	A	G	2: 121,256,641	L178P	probably damaging	Het
Trp63	A	G	16: 25,866,218	*285W	probably null	Het
Tsen34	T	C	7: 3,694,381		probably benign	Het
Tssk4	C	T	14: 55,651,815	T256I	probably benign	Het
Ttc17	A	T	2: 94,366,609	N464K	probably damaging	Het
Ttll2	C	T	17: 7,351,599	V310I	probably benign	Het
Ubn1	A	G	16: 5,077,313	E741G	probably damaging	Het
Urad	T	A	5: 147,315,454	I63F	probably damaging	Het
Vcan	T	C	13: 89,704,739	T701A	probably benign	Het
Vmn2r58	A	G	7: 41,837,215	V752A	possibly damaging	Het
Vmn2r69	C	A	7: 85,411,585	V264L	possibly damaging	Het
Zfp292	A	G	4: 34,808,917	S1376P	probably damaging	Het
Zfp955a	G	A	17: 33,241,725	R478*	probably null	Het
Znfx1	A	C	2: 167,055,269	F578L	probably benign	Het

Other mutations in Cdc14a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00801:Cdc14a	APN	3	116294844	nonsense	probably null
IGL01062:Cdc14a	APN	3	116274712	splice site	probably benign
IGL01584:Cdc14a	APN	3	116392825	nonsense	probably null
IGL03084:Cdc14a	APN	3	116348452	critical splice donor site	probably null
IGL03237:Cdc14a	APN	3	116404626	intron	probably benign
IGL03296:Cdc14a	APN	3	116297158	missense	probably benign	0.02
PIT4131001:Cdc14a	UTSW	3	116328661	missense	possibly damaging	0.66
R0707:Cdc14a	UTSW	3	116293713	splice site	probably benign
R0782:Cdc14a	UTSW	3	116322136	missense	probably damaging	1.00
R0835:Cdc14a	UTSW	3	116328522	missense	probably benign	0.12
R1363:Cdc14a	UTSW	3	116293860	small deletion	probably benign
R1507:Cdc14a	UTSW	3	116293997	missense	possibly damaging	0.47
R1545:Cdc14a	UTSW	3	116293724	critical splice donor site	probably null
R1795:Cdc14a	UTSW	3	116298473	missense	possibly damaging	0.81
R1797:Cdc14a	UTSW	3	116322194	missense	probably damaging	1.00
R1830:Cdc14a	UTSW	3	116422647	nonsense	probably null
R4229:Cdc14a	UTSW	3	116293764	missense	probably damaging	0.99
R4655:Cdc14a	UTSW	3	116328487	missense	probably damaging	1.00
R4769:Cdc14a	UTSW	3	116294750	critical splice donor site	probably null
R4980:Cdc14a	UTSW	3	116392857	nonsense	probably null
R6228:Cdc14a	UTSW	3	116351213	missense	probably damaging	1.00
R6248:Cdc14a	UTSW	3	116308194	missense	probably benign	0.01
R6402:Cdc14a	UTSW	3	116348459	missense	probably damaging	1.00
R6749:Cdc14a	UTSW	3	116297158	missense	possibly damaging	0.68
R6852:Cdc14a	UTSW	3	116328676	missense	possibly damaging	0.94
R6996:Cdc14a	UTSW	3	116328706	missense	probably damaging	1.00
R7185:Cdc14a	UTSW	3	116294027	missense	probably benign
R7783:Cdc14a	UTSW	3	116404587	missense	probably damaging	1.00
R7896:Cdc14a	UTSW	3	116294833	missense	probably benign	0.00
R7991:Cdc14a	UTSW	3	116308238	missense	probably benign	0.01
R8049:Cdc14a	UTSW	3	116293928	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- ATCCCAAGCAGAGAAGCCTG -3'
(R):5'- CCATGATCACTTTGGGAGCC -3'

Sequencing Primer
(F):5'- AGAGAAGCCTGCACTGTCC -3'
(R):5'- AAAGACTTTGCCCGGCCTTG -3'

Posted On

2016-03-17