|Institutional Source||Beutler Lab|
|Gene Name||RAD54 like (S. cerevisiae)|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R6369 (G1)|
|Chromosomal Location||116094264-116123690 bp(-) (GRCm38)|
|Type of Mutation||critical splice donor site (2 bp from exon)|
|DNA Base Change (assembly)||A to G at 116111189 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000099766 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000102704] [ENSMUST00000102705]|
|Coding Region Coverage||
|Validation Efficiency||98% (54/55)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008]
PHENOTYPE: Mice homozygous for a knock-out allele are sensitive to DNA crosslinking agents and to ionizing radiation and show abnormal definitive hematopoiesis following X-ray treatment. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Rad54l||
(F):5'- GTGAGGAACCCCATAACCTG -3'
(R):5'- TACATGAGGGATGGGTCACG -3'
(F):5'- TGAGGAACCCCATAACCTGAACAC -3'
(R):5'- TCACGTTGGGCCTGCTAGAG -3'