Incidental Mutation 'PIT4469001:Dusp16'
ID 555825
Institutional Source Beutler Lab
Gene Symbol Dusp16
Ensembl Gene ENSMUSG00000030203
Gene Name dual specificity phosphatase 16
Synonyms MKP-7, MKP7, D6Ertd213e, 3830417M17Rik
Accession Numbers
Essential gene? Probably essential (E-score: 0.890) question?
Stock # PIT4469001 (G1)
Quality Score 131.008
Status Not validated
Chromosome 6
Chromosomal Location 134692431-134769588 bp(-) (GRCm39)
Type of Mutation unclassified
DNA Base Change (assembly) G to A at 134738115 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000144834 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000100857] [ENSMUST00000129433] [ENSMUST00000149776] [ENSMUST00000204083]
AlphaFold Q6PCP3
Predicted Effect probably benign
Transcript: ENSMUST00000100857
SMART Domains Protein: ENSMUSP00000098419
Gene: ENSMUSG00000030203

RHOD 12 134 5.58e-16 SMART
DSPc 158 297 1.66e-68 SMART
Blast:DSPc 576 621 9e-11 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000129433
SMART Domains Protein: ENSMUSP00000115925
Gene: ENSMUSG00000030203

Blast:RHOD 1 67 8e-41 BLAST
PDB:2VSW|B 1 83 1e-52 PDB
DSPc 91 232 3.73e-44 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000148926
Predicted Effect probably benign
Transcript: ENSMUST00000149776
SMART Domains Protein: ENSMUSP00000144784
Gene: ENSMUSG00000030203

RHOD 12 134 5.58e-16 SMART
Blast:DSPc 158 203 2e-6 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000204083
SMART Domains Protein: ENSMUSP00000144834
Gene: ENSMUSG00000030203

RHOD 12 124 1.5e-8 SMART
Coding Region Coverage
  • 1x: 93.3%
  • 3x: 90.7%
  • 10x: 84.4%
  • 20x: 71.1%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.[provided by RefSeq, May 2010]
PHENOTYPE: Mice homozygous for a gene trap allele exhibit complete neonatal lethality and decreased birth weight. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 29 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A630001G21Rik A G 1: 85,652,920 (GRCm39) F83L probably benign Het
Acsm2 T A 7: 119,177,408 (GRCm39) C308S possibly damaging Het
Ak3 A G 19: 29,025,157 (GRCm39) S25P probably damaging Het
Atp13a2 T A 4: 140,721,438 (GRCm39) V176E unknown Het
Bmper A T 9: 23,317,845 (GRCm39) H488L probably benign Het
Cacna1c A T 6: 118,572,933 (GRCm39) C2084S unknown Het
Ccp110 T A 7: 118,321,600 (GRCm39) N418K probably benign Het
Ddx17 C A 15: 79,428,014 (GRCm39) G32C probably damaging Het
Dennd2b G A 7: 109,130,337 (GRCm39) A888V probably damaging Het
Efl1 T G 7: 82,307,373 (GRCm39) F90V probably benign Het
Ell2 T A 13: 75,910,011 (GRCm39) N252K probably damaging Het
Gdf6 T C 4: 9,859,569 (GRCm39) V217A probably damaging Het
H1f4 A T 13: 23,806,362 (GRCm39) V40E probably damaging Het
Hint1 T A 11: 54,760,896 (GRCm39) S112T unknown Het
Kif5c T C 2: 49,631,360 (GRCm39) V679A probably benign Het
Lrrn3 A T 12: 41,503,017 (GRCm39) D433E probably benign Het
Mast4 G A 13: 102,941,226 (GRCm39) T277M probably damaging Het
Naa11 A G 5: 97,539,485 (GRCm39) probably null Het
Pcdh18 T A 3: 49,709,518 (GRCm39) H599L probably benign Het
Pgc A T 17: 48,039,680 (GRCm39) K25* probably null Het
Pramel58 T A 5: 94,830,652 (GRCm39) V50E probably damaging Het
Psd4 G A 2: 24,284,306 (GRCm39) D57N probably benign Het
Pxdn G A 12: 30,055,828 (GRCm39) R1238Q probably benign Het
Smim41 T C 15: 101,191,152 (GRCm39) V6A probably benign Het
Spata32 T C 11: 103,100,653 (GRCm39) N38S probably benign Het
Tpr A G 1: 150,279,707 (GRCm39) T279A probably benign Het
Unc13a C A 8: 72,110,958 (GRCm39) E418* probably null Het
Vmn2r97 C A 17: 19,149,878 (GRCm39) T422K probably benign Het
Zfp474 A T 18: 52,771,791 (GRCm39) Q148L possibly damaging Het
Other mutations in Dusp16
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01090:Dusp16 APN 6 134,702,912 (GRCm39) missense probably benign 0.13
IGL01305:Dusp16 APN 6 134,695,824 (GRCm39) missense probably benign 0.03
IGL01331:Dusp16 APN 6 134,695,067 (GRCm39) missense possibly damaging 0.53
IGL02535:Dusp16 APN 6 134,695,790 (GRCm39) missense probably benign
IGL02606:Dusp16 APN 6 134,737,999 (GRCm39) missense probably damaging 0.96
IGL02696:Dusp16 APN 6 134,695,398 (GRCm39) missense probably damaging 0.99
ANU22:Dusp16 UTSW 6 134,695,824 (GRCm39) missense probably benign 0.03
PIT4504001:Dusp16 UTSW 6 134,716,846 (GRCm39) missense possibly damaging 0.90
R0492:Dusp16 UTSW 6 134,695,365 (GRCm39) missense probably benign
R0578:Dusp16 UTSW 6 134,695,284 (GRCm39) missense probably damaging 1.00
R1630:Dusp16 UTSW 6 134,697,524 (GRCm39) missense probably damaging 1.00
R1962:Dusp16 UTSW 6 134,695,099 (GRCm39) nonsense probably null
R2004:Dusp16 UTSW 6 134,695,802 (GRCm39) missense probably benign
R3690:Dusp16 UTSW 6 134,738,082 (GRCm39) unclassified probably benign
R3730:Dusp16 UTSW 6 134,695,824 (GRCm39) missense probably benign
R5778:Dusp16 UTSW 6 134,695,277 (GRCm39) missense probably benign 0.01
R6267:Dusp16 UTSW 6 134,697,456 (GRCm39) critical splice donor site probably null
R6296:Dusp16 UTSW 6 134,697,456 (GRCm39) critical splice donor site probably null
R6860:Dusp16 UTSW 6 134,702,842 (GRCm39) nonsense probably null
R7248:Dusp16 UTSW 6 134,695,940 (GRCm39) missense probably benign 0.01
R7645:Dusp16 UTSW 6 134,702,888 (GRCm39) missense probably damaging 0.97
R8108:Dusp16 UTSW 6 134,716,836 (GRCm39) missense probably benign
R8743:Dusp16 UTSW 6 134,694,933 (GRCm39) missense probably benign 0.35
R8824:Dusp16 UTSW 6 134,716,732 (GRCm39) missense probably benign
R8934:Dusp16 UTSW 6 134,718,639 (GRCm39) intron probably benign
R9328:Dusp16 UTSW 6 134,716,902 (GRCm39) missense probably damaging 1.00
R9364:Dusp16 UTSW 6 134,695,982 (GRCm39) missense probably damaging 1.00
R9430:Dusp16 UTSW 6 134,737,829 (GRCm39) missense probably damaging 1.00
R9476:Dusp16 UTSW 6 134,695,226 (GRCm39) missense probably benign 0.07
R9510:Dusp16 UTSW 6 134,695,226 (GRCm39) missense probably benign 0.07
R9598:Dusp16 UTSW 6 134,695,185 (GRCm39) missense probably benign 0.01
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2019-06-07