Mutagenetix > Incidental Mutation

Incidental Mutation 'R7797:Miip'

600270

Institutional Source

Beutler Lab

Gene Symbol

Miip

Ensembl Gene

ENSMUSG00000029022

Gene Name

migration and invasion inhibitory protein

Synonyms

D4Wsu114e

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R7797 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

147860778-147868816 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 147862918 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 236 (G236S)

Ref Sequence

ENSEMBL: ENSMUSP00000113897 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030886] [ENSMUST00000119975] [ENSMUST00000172710]

AlphaFold

A2A7Y5

Predicted Effect

probably benign
Transcript: ENSMUST00000030886
AA Change: G236S

PolyPhen 2 Score 0.205 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000030886
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000119975
AA Change: G236S

PolyPhen 2 Score 0.171 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000113897
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
Pfam:MIIP	41	382	1.4e-142	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000172710
AA Change: G236S

PolyPhen 2 Score 0.205 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000134085
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

100% (68/68)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that interacts with the oncogene protein insulin-like growth factor binding protein 2 and may function as an inhibitor of cell migration and invasion. This protein also interacts with the cell division protein 20 and may be involved in regulating mitotic progression. This protein may function as a tumor suppressor by inhibiting the growth or certain cancers. [provided by RefSeq, Sep 2011]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921501E09Rik	T	C	17: 33,067,690	D46G	probably damaging	Het
9230110C19Rik	G	T	9: 8,027,129	P136Q	possibly damaging	Het
Abca8b	A	G	11: 109,971,683		probably null	Het
Adam3	T	G	8: 24,694,644	N535T	probably damaging	Het
Adamts4	A	G	1: 171,257,818	K679E	probably damaging	Het
Arnt	G	A	3: 95,480,261		probably null	Het
Asah2	A	G	19: 32,022,361	F321L	probably damaging	Het
Asb15	T	C	6: 24,562,506	S156P	probably damaging	Het
Baiap2l1	A	C	5: 144,318,950	S65A	probably damaging	Het
Bend7	T	G	2: 4,749,644	M186R	probably damaging	Het
Blnk	T	C	19: 40,959,788	K146E	possibly damaging	Het
Cd19	A	G	7: 126,413,508	W238R	probably damaging	Het
Cd44	T	A	2: 102,848,734	N241I	probably benign	Het
Cdh23	T	C	10: 60,385,194	E1257G	probably benign	Het
Cntnap5c	T	C	17: 58,359,275	V1100A	probably benign	Het
Cul7	T	C	17: 46,658,642	V945A	possibly damaging	Het
Dock2	C	T	11: 34,282,652	C1116Y	probably damaging	Het
Ehbp1	T	C	11: 22,096,109	M547V	possibly damaging	Het
Fam50b	G	A	13: 34,747,101	E187K	possibly damaging	Het
Gm5346	T	A	8: 43,626,374	E271V	probably benign	Het
Grhl3	C	G	4: 135,559,105	K88N	possibly damaging	Het
Hbb-y	T	A	7: 103,851,881	Y146F	probably damaging	Het
Hivep2	T	C	10: 14,130,103	V815A	probably benign	Het
Hoxa7	T	A	6: 52,215,890	I173F	probably damaging	Het
Igkv1-110	T	G	6: 68,270,993	S29A	probably benign	Het
Jade2	A	G	11: 51,817,299	S696P	probably benign	Het
Kif1b	T	A	4: 149,237,387	Q1025L	probably benign	Het
Kif2c	A	T	4: 117,171,743	C241S	probably benign	Het
Kmt2d	G	T	15: 98,864,406	H400N	probably benign	Het
Krt35	T	A	11: 100,094,887	N174Y	probably damaging	Het
Ldlrad1	G	A	4: 107,209,491	A8T	probably benign	Het
Magi3	A	G	3: 104,051,302	V489A	probably damaging	Het
Mapk7	T	C	11: 61,489,415	E739G	possibly damaging	Het
Mcidas	G	A	13: 112,998,987	G315S	probably damaging	Het
Mdga1	C	T	17: 29,842,840		probably null	Het
Megf8	G	A	7: 25,334,597	R607H	probably damaging	Het
Met	T	A	6: 17,533,953	N634K	probably damaging	Het
Mmp11	G	T	10: 75,923,480	T107K		Het
Mroh2b	A	C	15: 4,949,105	N1378H	probably benign	Het
Naa15	G	A	3: 51,448,610	C322Y	probably damaging	Het
Naip1	T	A	13: 100,444,478	Y87F	probably damaging	Het
Ngrn	A	G	7: 80,264,437	D112G	probably benign	Het
Osbpl1a	C	A	18: 12,882,264	C369F	probably damaging	Het
Pcnx2	G	C	8: 125,785,348	D1406E	possibly damaging	Het
Pold1	G	A	7: 44,541,789	P206L	probably benign	Het
Psen1	A	G	12: 83,699,622	S20G	probably benign	Het
Ptpn23	C	T	9: 110,393,807	D61N	possibly damaging	Het
Rbfox3	A	T	11: 118,496,484	L268Q	possibly damaging	Het
Rbm22	A	G	18: 60,561,272	T26A	probably damaging	Het
Rpgrip1	G	A	14: 52,133,820	R332Q	possibly damaging	Het
Rrm2b	A	T	15: 37,927,261	L347*	probably null	Het
Rsf1	A	T	7: 97,661,485	D474V		Het
Ryr2	C	T	13: 11,801,180	R640Q	probably damaging	Het
Sec16b	A	G	1: 157,561,675	E691G	unknown	Het
Sept1	A	T	7: 127,214,765	V365E	unknown	Het
Tlk2	A	G	11: 105,210,618	H114R	probably benign	Het
Tmem200a	A	G	10: 25,993,966	I135T	possibly damaging	Het
Tpst2	T	A	5: 112,307,916	V107E	probably damaging	Het
Trim30a	A	T	7: 104,411,200	D456E	possibly damaging	Het
Ttll3	T	C	6: 113,394,777	V45A	possibly damaging	Het
Ulk2	A	G	11: 61,782,102	Y889H	probably benign	Het
Umodl1	A	G	17: 30,959,151	S34G	probably benign	Het
Vmn1r67	A	G	7: 10,446,976	I56V	probably benign	Het
Vmn2r120	C	T	17: 57,508,874	G827E	probably damaging	Het
Zc3h6	T	C	2: 129,015,635		probably null	Het

Other mutations in Miip

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00531:Miip	APN	4	147865865	missense	probably damaging	1.00
IGL02134:Miip	APN	4	147865278	splice site	probably benign
IGL02829:Miip	APN	4	147863061	missense	probably benign	0.01
IGL03350:Miip	APN	4	147862522	missense	probably benign	0.01
R0200:Miip	UTSW	4	147862263	missense	probably damaging	0.99
R1647:Miip	UTSW	4	147865234	missense	probably benign	0.02
R1783:Miip	UTSW	4	147865774	missense	probably damaging	1.00
R1848:Miip	UTSW	4	147863092	missense	probably damaging	0.99
R1944:Miip	UTSW	4	147865965	missense	probably benign	0.15
R3615:Miip	UTSW	4	147865914	missense	probably benign	0.00
R3616:Miip	UTSW	4	147865914	missense	probably benign	0.00
R3882:Miip	UTSW	4	147861052	missense	possibly damaging	0.93
R4579:Miip	UTSW	4	147861061	missense	probably damaging	1.00
R5183:Miip	UTSW	4	147863069	missense	probably damaging	1.00
R6054:Miip	UTSW	4	147865678	missense	probably benign	0.00
R6056:Miip	UTSW	4	147862335	missense	probably damaging	1.00
R6304:Miip	UTSW	4	147863083	missense	probably benign	0.12
R6568:Miip	UTSW	4	147865915	missense	probably benign
R6603:Miip	UTSW	4	147865923	missense	possibly damaging	0.92
R7639:Miip	UTSW	4	147862564	missense	probably benign	0.22
R7701:Miip	UTSW	4	147862914	missense	probably null	0.86
R7795:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7796:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7872:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7920:Miip	UTSW	4	147862918	missense	probably benign	0.17
R8468:Miip	UTSW	4	147861471	missense	probably damaging	1.00
R8492:Miip	UTSW	4	147861424	missense	probably damaging	1.00
R8677:Miip	UTSW	4	147863046	missense	probably damaging	1.00
R8852:Miip	UTSW	4	147866382	start gained	probably benign
R8860:Miip	UTSW	4	147866382	start gained	probably benign
R9755:Miip	UTSW	4	147865862	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGGGAGAAATGTCCTCCCAG -3'
(R):5'- GGACAATAGCTCTCCAGTCACTAG -3'

Sequencing Primer
(F):5'- AAATGTCCTCCCAGGGGCAG -3'
(R):5'- ATAGCTCTCCAGTCACTAGTGAGC -3'

Posted On

2019-11-26