Mutagenetix > Incidental Mutation

Incidental Mutation 'R8400:Acot10'

647832

Institutional Source

Beutler Lab

Gene Symbol

Acot10

Ensembl Gene

ENSMUSG00000047565

Gene Name

acyl-CoA thioesterase 10

Synonyms

p48, MT-ACT48, Acate3

MMRRC Submission

067763-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.147) question?

Stock #

R8400 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

20665300-20666836 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 20666258 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 161 (E161G)

Ref Sequence

ENSEMBL: ENSMUSP00000051333 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052910]

AlphaFold

Q32MW3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000052910
AA Change: E161G

PolyPhen 2 Score 0.556 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000051333
Gene: ENSMUSG00000047565
AA Change: E161G

Domain	Start	End	E-Value	Type
SCOP:d1lo7a_	108	222	1e-4	SMART
PDB:4IEN\|D	277	400	3e-6	PDB

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	G	A	11: 9,243,925 (GRCm39)	M1929I	probably benign	Het
Abca13	T	C	11: 9,248,218 (GRCm39)	I2655T	probably damaging	Het
Acad10	A	G	5: 121,764,268 (GRCm39)	V887A	possibly damaging	Het
Astn1	C	T	1: 158,484,670 (GRCm39)	P919L	probably benign	Het
Atp1a4	T	A	1: 172,062,061 (GRCm39)	D688V	probably damaging	Het
C4bp	C	A	1: 130,564,484 (GRCm39)	C400F	probably damaging	Het
Col6a6	A	C	9: 105,651,995 (GRCm39)	D1005E	probably damaging	Het
Csnk1g3	C	T	18: 54,086,360 (GRCm39)	R422C	probably benign	Het
Cutc	T	C	19: 43,741,644 (GRCm39)	S15P	probably benign	Het
Dgkb	T	C	12: 38,652,837 (GRCm39)		probably null	Het
Disc1	T	C	8: 125,959,732 (GRCm39)	V748A	probably benign	Het
Dmbt1	C	G	7: 130,684,317 (GRCm39)	D778E	unknown	Het
Dmxl2	T	C	9: 54,291,037 (GRCm39)	Y2471C	probably benign	Het
Fam185a	T	A	5: 21,643,814 (GRCm39)	N243K	probably benign	Het
Fchsd2	A	T	7: 100,902,780 (GRCm39)	Q386L	possibly damaging	Het
Gm904	C	A	13: 50,797,453 (GRCm39)	P49Q	probably damaging	Het
H2-Q10	C	T	17: 35,781,374 (GRCm39)	R59C	probably damaging	Het
Ier5l	A	G	2: 30,363,105 (GRCm39)	Y307H	possibly damaging	Het
Kmt2e	C	A	5: 23,702,090 (GRCm39)	T906K	probably benign	Het
Kndc1	C	T	7: 139,493,434 (GRCm39)	R467W	probably damaging	Het
Muc5ac	C	A	7: 141,364,213 (GRCm39)	T2508K	probably damaging	Het
Nlrp9a	A	T	7: 26,264,431 (GRCm39)	M784L	probably benign	Het
Nlrp9b	T	A	7: 19,757,937 (GRCm39)	C391*	probably null	Het
Nubp2	A	C	17: 25,103,439 (GRCm39)	M146R	probably damaging	Het
Or10q3	T	A	19: 11,848,578 (GRCm39)	M1L	probably damaging	Het
Or1a1b	C	T	11: 74,097,221 (GRCm39)	V274M	possibly damaging	Het
Or2ag1b	T	A	7: 106,288,876 (GRCm39)	S21C	probably benign	Het
Or2b2	G	A	13: 21,888,085 (GRCm39)	V305M	probably benign	Het
Or4k45	A	T	2: 111,395,747 (GRCm39)	L14H	probably damaging	Het
Or7e166	A	T	9: 19,624,389 (GRCm39)	N89Y	probably benign	Het
Otud1	T	C	2: 19,663,189 (GRCm39)	V106A	possibly damaging	Het
Pcdhac1	T	A	18: 37,225,453 (GRCm39)	Y755*	probably null	Het
Pkd1l3	C	T	8: 110,350,520 (GRCm39)	P455L	possibly damaging	Het
Ptprb	GAGACCCTCGGGAGCACTGCAGAGACCCTCAGGAACACTGCAAAGACCCTCGGGAGCACTGCAGAGACCCTCAGGAACACTGCAAAGACCCTCGGGAGCACTGCAGAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACT	GAGACCCTCGGGAGCACTGCAGAGACCCTCAGGAACACTGCAAAGACCCTCGGGAGCACTGCAGAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACT	10: 116,119,477 (GRCm39)		probably benign	Het
Samhd1	T	C	2: 156,941,353 (GRCm39)	E648G	probably benign	Het
Smarca5	T	C	8: 81,435,756 (GRCm39)	T794A	probably benign	Het
Spc24	A	T	9: 21,669,026 (GRCm39)	L87H	probably damaging	Het
Sppl2b	TGTCACAGGT	TGT	10: 80,701,903 (GRCm39)		probably null	Het
Stra6l	G	A	4: 45,864,905 (GRCm39)	R77Q	probably damaging	Het
Tdrd1	G	A	19: 56,837,081 (GRCm39)	V472M	probably benign	Het
Tsc2	A	T	17: 24,823,961 (GRCm39)	I948K	possibly damaging	Het
Ttc39d	T	C	17: 80,523,434 (GRCm39)	V31A	probably benign	Het
Vmn1r158	A	T	7: 22,489,305 (GRCm39)	C301*	probably null	Het
Vmn2r22	A	T	6: 123,614,486 (GRCm39)	L368*	probably null	Het
Vmn2r79	A	G	7: 86,651,308 (GRCm39)	T236A	probably benign	Het
Vwa1	T	C	4: 155,857,225 (GRCm39)	H191R	probably benign	Het
Zdbf2	T	A	1: 63,344,135 (GRCm39)	V838E	possibly damaging	Het

Other mutations in Acot10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01306:Acot10	APN	15	20,666,051 (GRCm39)	missense	probably benign	0.11
IGL01610:Acot10	APN	15	20,665,781 (GRCm39)	missense	probably damaging	1.00
IGL02457:Acot10	APN	15	20,666,229 (GRCm39)	missense	possibly damaging	0.88
IGL02587:Acot10	APN	15	20,665,883 (GRCm39)	missense	possibly damaging	0.93
IGL02951:Acot10	APN	15	20,665,868 (GRCm39)	missense	probably benign	0.36
ANU23:Acot10	UTSW	15	20,666,051 (GRCm39)	missense	probably benign	0.11
PIT4151001:Acot10	UTSW	15	20,666,684 (GRCm39)	missense	probably damaging	0.98
R0026:Acot10	UTSW	15	20,666,322 (GRCm39)	missense	probably benign	0.10
R0026:Acot10	UTSW	15	20,666,322 (GRCm39)	missense	probably benign	0.10
R0462:Acot10	UTSW	15	20,666,712 (GRCm39)	missense	possibly damaging	0.85
R1312:Acot10	UTSW	15	20,666,585 (GRCm39)	missense	probably benign	0.00
R1495:Acot10	UTSW	15	20,665,593 (GRCm39)	missense	probably damaging	0.99
R2128:Acot10	UTSW	15	20,666,712 (GRCm39)	missense	probably benign	0.00
R3779:Acot10	UTSW	15	20,665,628 (GRCm39)	missense	probably damaging	0.98
R4110:Acot10	UTSW	15	20,666,612 (GRCm39)	missense	probably damaging	1.00
R4111:Acot10	UTSW	15	20,666,612 (GRCm39)	missense	probably damaging	1.00
R4464:Acot10	UTSW	15	20,665,830 (GRCm39)	nonsense	probably null
R4668:Acot10	UTSW	15	20,666,028 (GRCm39)	missense	probably benign
R4933:Acot10	UTSW	15	20,666,416 (GRCm39)	missense	possibly damaging	0.88
R5255:Acot10	UTSW	15	20,666,018 (GRCm39)	missense	probably benign	0.01
R5885:Acot10	UTSW	15	20,666,190 (GRCm39)	missense	probably benign	0.01
R6190:Acot10	UTSW	15	20,665,871 (GRCm39)	missense	possibly damaging	0.80
R6301:Acot10	UTSW	15	20,666,348 (GRCm39)	missense	probably benign	0.05
R6805:Acot10	UTSW	15	20,665,452 (GRCm39)	missense	probably benign	0.42
R7334:Acot10	UTSW	15	20,665,629 (GRCm39)	missense	possibly damaging	0.86
R7601:Acot10	UTSW	15	20,665,715 (GRCm39)	missense	probably damaging	1.00
R9195:Acot10	UTSW	15	20,665,517 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTGGATTTACAAATGCCGGC -3'
(R):5'- ACATTGAAGTTCTCCTACCTTTGGG -3'

Sequencing Primer
(F):5'- GGCCTCTTATTTTCAGAATCTTGAG -3'
(R):5'- GGTACTGACCCTGAACTACGAG -3'

Posted On

2020-09-02