Mutagenetix > Incidental Mutation

Incidental Mutation 'R4860:Vps13d'

372392

Institutional Source

Beutler Lab

Gene Symbol

Vps13d

Ensembl Gene

ENSMUSG00000020220

Gene Name

vacuolar protein sorting 13D

Synonyms

MMRRC Submission

042471-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4860 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

144972622-145195005 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 145087161 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 165 (F165L)

Ref Sequence

ENSEMBL: ENSMUSP00000118699 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020441] [ENSMUST00000036579] [ENSMUST00000130704]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000020441
AA Change: F3249L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000020441
Gene: ENSMUSG00000020220
AA Change: F3249L

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	118	1.8e-37	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
coiled coil region	665	685	N/A	INTRINSIC
low complexity region	765	781	N/A	INTRINSIC
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2866	2884	N/A	INTRINSIC
low complexity region	2973	2983	N/A	INTRINSIC
Pfam:DUF1162	3246	3530	1.1e-110	PFAM
low complexity region	3797	3810	N/A	INTRINSIC
low complexity region	3913	3921	N/A	INTRINSIC
low complexity region	4119	4132	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000036579
AA Change: F3280L

SMART Domains

Protein: ENSMUSP00000043240
Gene: ENSMUSG00000020220
AA Change: F3280L

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	116	3.5e-35	PFAM
Pfam:VPS13	131	353	9.6e-57	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
Pfam:VPS13_mid_rpt	608	896	4.3e-35	PFAM
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2891	2909	N/A	INTRINSIC
low complexity region	2998	3008	N/A	INTRINSIC
Pfam:SHR-BD	3271	3555	4.2e-86	PFAM
low complexity region	3822	3835	N/A	INTRINSIC
low complexity region	3938	3946	N/A	INTRINSIC
Pfam:VPS13_C	3978	4126	4.8e-24	PFAM
low complexity region	4144	4157	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000130704
AA Change: F165L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000118699
Gene: ENSMUSG00000020220
AA Change: F165L

Domain	Start	End	E-Value	Type
Pfam:DUF1162	162	446	1.4e-111	PFAM
low complexity region	713	726	N/A	INTRINSIC
low complexity region	829	837	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000185113
AA Change: F2100L

Meta Mutation Damage Score

0.0577

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.0%
20x: 87.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit embryonic lethality at E7. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700030K09Rik	T	C	8: 72,455,423 (GRCm38)	S466P	possibly damaging	Het
Ablim1	T	C	19: 57,079,866 (GRCm38)	T267A	probably damaging	Het
Acap2	A	T	16: 31,103,499 (GRCm38)	L724Q	possibly damaging	Het
Acsbg3	A	G	17: 56,888,655 (GRCm38)	N684S	probably benign	Het
Adcy4	T	C	14: 55,781,927 (GRCm38)	T89A	possibly damaging	Het
Agrp	T	C	8: 105,567,368 (GRCm38)	E41G	probably benign	Het
Ajm1	T	C	2: 25,578,753 (GRCm38)	Y382C	probably damaging	Het
Akr1d1	G	A	6: 37,564,491 (GRCm38)	V308M	probably damaging	Het
Ap1m2	C	T	9: 21,309,674 (GRCm38)	R54Q	probably benign	Het
Arhgap45	T	A	10: 80,027,066 (GRCm38)	V692E	probably damaging	Het
Arid5b	G	T	10: 68,243,095 (GRCm38)	N137K	probably damaging	Het
Arsi	G	A	18: 60,916,651 (GRCm38)	G202E	probably benign	Het
BC048679	T	C	7: 81,495,720 (GRCm38)	N27D	probably benign	Het
Ccdc78	A	G	17: 25,788,700 (GRCm38)	N237S	probably benign	Het
Cd46	G	A	1: 195,062,396 (GRCm38)	L345F	possibly damaging	Het
Cngb3	A	T	4: 19,425,569 (GRCm38)	N459I	possibly damaging	Het
Ctnnd2	A	G	15: 30,881,167 (GRCm38)	E731G	probably damaging	Het
Ctsh	A	G	9: 90,054,548 (GRCm38)	E26G	probably benign	Het
Cul1	G	T	6: 47,517,146 (GRCm38)	K464N	probably benign	Het
Cul1	T	A	6: 47,517,191 (GRCm38)	S479R	probably damaging	Het
Dcaf5	A	T	12: 80,340,232 (GRCm38)	D373E	probably benign	Het
Dhcr7	A	G	7: 143,840,500 (GRCm38)	Q126R	probably benign	Het
Dok2	T	C	14: 70,777,516 (GRCm38)	F228L	probably damaging	Het
Dpep3	T	G	8: 105,976,189 (GRCm38)	I314L	probably benign	Het
Eps8	A	G	6: 137,514,295 (GRCm38)	F362L	probably damaging	Het
Espn	G	T	4: 152,138,846 (GRCm38)	R250S	probably damaging	Het
Faf1	A	C	4: 109,742,896 (GRCm38)	N163H	probably damaging	Het
Fcho1	C	T	8: 71,710,481 (GRCm38)	V635I	probably benign	Het
Garin5b	C	T	7: 4,757,469 (GRCm38)		probably null	Het
Gask1b	C	A	3: 79,936,674 (GRCm38)	S36*	probably null	Het
Gm7579	G	A	7: 142,211,908 (GRCm38)	C17Y	unknown	Het
Gpx8	G	T	13: 113,045,508 (GRCm38)	Y130*	probably null	Het
Gvin1	A	T	7: 106,163,436 (GRCm38)	Y609N	possibly damaging	Het
Hectd4	T	A	5: 121,305,818 (GRCm38)	M30K	probably benign	Het
Iqub	T	C	6: 24,450,842 (GRCm38)	D586G	probably damaging	Het
Klhl25	T	C	7: 75,867,050 (GRCm38)	I568T	probably benign	Het
Larp6	A	G	9: 60,737,810 (GRCm38)	E411G	probably damaging	Het
Lepr	A	C	4: 101,789,337 (GRCm38)	I822L	probably benign	Het
Lrig3	C	A	10: 126,011,052 (GRCm38)	D896E	probably benign	Het
Lrp1	C	T	10: 127,553,824 (GRCm38)	G3114D	probably damaging	Het
Lyset	A	T	12: 102,744,055 (GRCm38)		probably benign	Het
Macf1	A	T	4: 123,486,750 (GRCm38)	Y1263N	probably damaging	Het
Mapk10	T	C	5: 102,990,619 (GRCm38)	D180G	probably damaging	Het
Matr3	T	A	18: 35,581,640 (GRCm38)	V113E	probably damaging	Het
Mbd4	A	T	6: 115,848,926 (GRCm38)	F368Y	possibly damaging	Het
Mcpt8	G	A	14: 56,082,280 (GRCm38)	R238W	probably benign	Het
Mcrip2	G	T	17: 25,864,647 (GRCm38)	T86N	possibly damaging	Het
Mink1	A	G	11: 70,611,592 (GRCm38)	N1043S	probably damaging	Het
Muc4	G	A	16: 32,754,616 (GRCm38)	G1497R	probably benign	Het
Muc4	T	A	16: 32,754,625 (GRCm38)	S1500T	probably benign	Het
Nbeal2	G	A	9: 110,635,194 (GRCm38)	T1128I	probably benign	Het
Nrg2	T	A	18: 36,196,547 (GRCm38)	Y205F	probably damaging	Het
Nubp2	A	G	17: 24,884,456 (GRCm38)	M149T	probably benign	Het
Or4d2b	T	A	11: 87,889,225 (GRCm38)	M224L	probably damaging	Het
Or8d6	G	T	9: 39,942,504 (GRCm38)	M81I	probably benign	Het
Or8j3c	T	C	2: 86,422,957 (GRCm38)	T240A	probably damaging	Het
Pax3	A	G	1: 78,192,456 (GRCm38)	I191T	possibly damaging	Het
Pdcd5	T	C	7: 35,643,710 (GRCm38)	N137D	possibly damaging	Het
Pik3c2a	G	A	7: 116,340,156 (GRCm38)	A1649V	probably damaging	Het
Pkhd1l1	T	C	15: 44,537,378 (GRCm38)	S2183P	possibly damaging	Het
Plekho1	T	A	3: 95,988,993 (GRCm38)	Q388L	possibly damaging	Het
Ppfibp1	A	G	6: 146,990,514 (GRCm38)	T91A	probably benign	Het
Ptger4	G	T	15: 5,242,606 (GRCm38)	N177K	probably benign	Het
Reln	A	G	5: 21,901,751 (GRCm38)	F3207S	probably benign	Het
Rigi	G	T	4: 40,210,000 (GRCm38)	S644R	probably damaging	Het
Ripk4	C	T	16: 97,751,536 (GRCm38)	R194H	probably damaging	Het
Rnf112	A	T	11: 61,452,744 (GRCm38)	C112S	possibly damaging	Het
Rprd1b	T	G	2: 158,074,935 (GRCm38)	Y278*	probably null	Het
Sel1l	A	G	12: 91,831,602 (GRCm38)	L140P	probably damaging	Het
Shroom3	G	T	5: 92,943,086 (GRCm38)	V1151F	probably damaging	Het
Slc38a3	A	G	9: 107,655,064 (GRCm38)	V423A	probably damaging	Het
Slitrk6	T	C	14: 110,751,883 (GRCm38)	T131A	probably damaging	Het
Slmap	A	T	14: 26,460,209 (GRCm38)	V323E	probably benign	Het
Smim6	T	C	11: 115,913,504 (GRCm38)	V39A	probably benign	Het
Sorbs1	T	C	19: 40,337,005 (GRCm38)	T382A	probably benign	Het
Sparc	G	A	11: 55,399,211 (GRCm38)	T218I	possibly damaging	Het
Steap1	A	T	5: 5,736,589 (GRCm38)	F283I	probably damaging	Het
Stil	A	G	4: 115,038,474 (GRCm38)	T586A	probably benign	Het
Tbce	T	A	13: 14,019,795 (GRCm38)	D93V	probably damaging	Het
Tcf12	C	T	9: 71,858,840 (GRCm38)	G504S	probably null	Het
Tex55	A	G	16: 38,828,145 (GRCm38)	S201P	probably damaging	Het
Tle4	A	T	19: 14,464,345 (GRCm38)	I435K	probably benign	Het
Tmem245	A	G	4: 56,899,164 (GRCm38)	F254S	probably damaging	Het
Tubgcp3	T	C	8: 12,649,722 (GRCm38)	K377R	probably benign	Het
Ush2a	A	T	1: 188,553,275 (GRCm38)	T2003S	probably benign	Het
Usp53	A	G	3: 122,961,363 (GRCm38)	S32P	possibly damaging	Het
Vmn1r78	T	A	7: 12,152,756 (GRCm38)	L98Q	probably damaging	Het
Vmn2r116	A	C	17: 23,401,803 (GRCm38)	Q837P	probably benign	Het
Vmn2r3	T	C	3: 64,275,601 (GRCm38)	I226V	probably benign	Het
Vmn2r84	T	A	10: 130,385,843 (GRCm38)	D836V	probably damaging	Het
Vstm4	A	G	14: 32,863,785 (GRCm38)	E103G	possibly damaging	Het
Zfp870	A	T	17: 32,883,340 (GRCm38)	C339*	probably null	Het

Other mutations in Vps13d

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Vps13d	APN	4	145,168,540 (GRCm38)	missense	probably damaging	0.98
IGL00484:Vps13d	APN	4	145,126,575 (GRCm38)	missense	probably benign	0.04
IGL00591:Vps13d	APN	4	145,190,559 (GRCm38)	missense	possibly damaging	0.95
IGL00816:Vps13d	APN	4	145,155,994 (GRCm38)	missense	probably benign	0.00
IGL00835:Vps13d	APN	4	145,160,652 (GRCm38)	missense	probably damaging	0.97
IGL00847:Vps13d	APN	4	145,085,408 (GRCm38)	missense	probably benign	0.26
IGL01084:Vps13d	APN	4	145,154,955 (GRCm38)	missense	probably benign	0.00
IGL01116:Vps13d	APN	4	144,972,750 (GRCm38)	unclassified	probably benign
IGL01150:Vps13d	APN	4	145,149,275 (GRCm38)	missense	probably benign
IGL01329:Vps13d	APN	4	145,156,206 (GRCm38)	missense	possibly damaging	0.69
IGL01338:Vps13d	APN	4	145,088,322 (GRCm38)	missense	probably damaging	1.00
IGL01583:Vps13d	APN	4	145,045,088 (GRCm38)	missense	probably damaging	1.00
IGL01598:Vps13d	APN	4	145,016,901 (GRCm38)	missense	probably benign	0.21
IGL01620:Vps13d	APN	4	145,094,867 (GRCm38)	missense	possibly damaging	0.70
IGL01636:Vps13d	APN	4	145,075,048 (GRCm38)	missense	probably damaging	1.00
IGL01723:Vps13d	APN	4	145,173,145 (GRCm38)	missense	possibly damaging	0.84
IGL01895:Vps13d	APN	4	145,156,266 (GRCm38)	missense	possibly damaging	0.57
IGL01981:Vps13d	APN	4	145,086,747 (GRCm38)	missense	probably damaging	0.99
IGL02192:Vps13d	APN	4	145,148,858 (GRCm38)	missense	probably benign	0.02
IGL02197:Vps13d	APN	4	145,128,309 (GRCm38)	missense	probably benign	0.01
IGL02209:Vps13d	APN	4	145,156,101 (GRCm38)	missense	probably damaging	0.97
IGL02219:Vps13d	APN	4	145,168,146 (GRCm38)	missense	probably benign	0.00
IGL02377:Vps13d	APN	4	145,156,364 (GRCm38)	missense	probably damaging	1.00
IGL02404:Vps13d	APN	4	145,148,735 (GRCm38)	missense	probably damaging	1.00
IGL02552:Vps13d	APN	4	145,173,137 (GRCm38)	missense	possibly damaging	0.46
IGL02651:Vps13d	APN	4	145,164,559 (GRCm38)	missense	probably benign	0.02
IGL02708:Vps13d	APN	4	145,128,280 (GRCm38)	missense	probably benign	0.12
IGL02811:Vps13d	APN	4	145,131,765 (GRCm38)	missense	possibly damaging	0.55
IGL02821:Vps13d	APN	4	145,148,762 (GRCm38)	missense	probably damaging	0.98
IGL02838:Vps13d	APN	4	145,075,025 (GRCm38)	missense	probably benign	0.31
IGL02968:Vps13d	APN	4	145,122,498 (GRCm38)	missense	probably benign	0.32
IGL03176:Vps13d	APN	4	145,074,963 (GRCm38)	missense	probably benign	0.16
IGL03352:Vps13d	APN	4	145,167,502 (GRCm38)	missense	possibly damaging	0.49
IGL03374:Vps13d	APN	4	145,108,575 (GRCm38)	missense	possibly damaging	0.70
IGL03375:Vps13d	APN	4	145,091,947 (GRCm38)	missense	probably damaging	1.00
IGL03383:Vps13d	APN	4	145,168,319 (GRCm38)	critical splice acceptor site	probably null
IGL03411:Vps13d	APN	4	145,149,324 (GRCm38)	missense	probably damaging	1.00
broken	UTSW	4	145,086,735 (GRCm38)	missense
demotion	UTSW	4	145,138,613 (GRCm38)	missense
BB008:Vps13d	UTSW	4	145,096,284 (GRCm38)	nonsense	probably null
BB018:Vps13d	UTSW	4	145,096,284 (GRCm38)	nonsense	probably null
PIT4283001:Vps13d	UTSW	4	145,108,588 (GRCm38)	missense
PIT4434001:Vps13d	UTSW	4	145,155,247 (GRCm38)	missense
R0069:Vps13d	UTSW	4	145,062,563 (GRCm38)	missense	probably benign	0.09
R0069:Vps13d	UTSW	4	145,062,563 (GRCm38)	missense	probably benign	0.09
R0076:Vps13d	UTSW	4	145,164,694 (GRCm38)	splice site	probably benign
R0211:Vps13d	UTSW	4	145,114,778 (GRCm38)	missense	probably benign	0.08
R0219:Vps13d	UTSW	4	145,105,909 (GRCm38)	missense	probably benign	0.01
R0284:Vps13d	UTSW	4	145,144,802 (GRCm38)	missense	probably benign	0.01
R0345:Vps13d	UTSW	4	145,117,625 (GRCm38)	missense	possibly damaging	0.81
R0400:Vps13d	UTSW	4	145,065,827 (GRCm38)	missense	probably benign	0.00
R0417:Vps13d	UTSW	4	144,976,560 (GRCm38)	missense	probably benign	0.19
R0538:Vps13d	UTSW	4	145,045,095 (GRCm38)	missense	probably damaging	1.00
R0560:Vps13d	UTSW	4	145,054,190 (GRCm38)	missense	probably damaging	1.00
R0627:Vps13d	UTSW	4	145,087,184 (GRCm38)	missense	probably damaging	1.00
R0707:Vps13d	UTSW	4	145,155,932 (GRCm38)	missense	probably damaging	1.00
R0782:Vps13d	UTSW	4	145,126,625 (GRCm38)	splice site	probably benign
R0925:Vps13d	UTSW	4	145,156,551 (GRCm38)	missense	probably damaging	1.00
R0993:Vps13d	UTSW	4	145,117,692 (GRCm38)	nonsense	probably null
R1135:Vps13d	UTSW	4	145,155,589 (GRCm38)	missense	probably benign	0.01
R1165:Vps13d	UTSW	4	145,126,471 (GRCm38)	missense	probably benign
R1263:Vps13d	UTSW	4	145,170,348 (GRCm38)	missense	probably benign	0.01
R1397:Vps13d	UTSW	4	145,141,334 (GRCm38)	missense	probably damaging	1.00
R1398:Vps13d	UTSW	4	145,099,983 (GRCm38)	missense	probably null
R1521:Vps13d	UTSW	4	145,105,861 (GRCm38)	missense	probably benign	0.00
R1522:Vps13d	UTSW	4	145,098,172 (GRCm38)	splice site	probably null
R1725:Vps13d	UTSW	4	145,143,260 (GRCm38)	missense	possibly damaging	0.90
R1759:Vps13d	UTSW	4	145,155,857 (GRCm38)	missense	probably benign
R1826:Vps13d	UTSW	4	145,155,003 (GRCm38)	missense	probably damaging	0.96
R1900:Vps13d	UTSW	4	145,126,606 (GRCm38)	missense	probably benign	0.23
R1943:Vps13d	UTSW	4	145,155,857 (GRCm38)	missense	probably benign
R1955:Vps13d	UTSW	4	145,156,143 (GRCm38)	missense	probably damaging	1.00
R2008:Vps13d	UTSW	4	145,155,243 (GRCm38)	missense	probably benign	0.00
R2013:Vps13d	UTSW	4	145,108,508 (GRCm38)	missense	probably damaging	0.99
R2014:Vps13d	UTSW	4	145,108,508 (GRCm38)	missense	probably damaging	0.99
R2038:Vps13d	UTSW	4	145,181,115 (GRCm38)	critical splice donor site	probably null
R2108:Vps13d	UTSW	4	145,075,047 (GRCm38)	missense	probably damaging	0.99
R2130:Vps13d	UTSW	4	145,156,101 (GRCm38)	missense	probably benign	0.17
R2134:Vps13d	UTSW	4	145,148,339 (GRCm38)	missense	probably benign	0.00
R2168:Vps13d	UTSW	4	145,087,323 (GRCm38)	splice site	probably benign
R2220:Vps13d	UTSW	4	145,178,320 (GRCm38)	missense	probably damaging	1.00
R2240:Vps13d	UTSW	4	145,110,895 (GRCm38)	missense	possibly damaging	0.70
R2332:Vps13d	UTSW	4	145,148,686 (GRCm38)	missense	probably benign
R2357:Vps13d	UTSW	4	145,074,977 (GRCm38)	frame shift	probably null
R2365:Vps13d	UTSW	4	145,087,324 (GRCm38)	splice site	probably benign
R2571:Vps13d	UTSW	4	145,149,136 (GRCm38)	missense	probably benign	0.20
R3149:Vps13d	UTSW	4	145,126,577 (GRCm38)	missense	possibly damaging	0.70
R3150:Vps13d	UTSW	4	145,086,790 (GRCm38)	missense	probably damaging	0.98
R3547:Vps13d	UTSW	4	145,074,975 (GRCm38)	missense	probably damaging	0.99
R3716:Vps13d	UTSW	4	145,075,726 (GRCm38)	missense	probably damaging	1.00
R3718:Vps13d	UTSW	4	145,075,726 (GRCm38)	missense	probably damaging	1.00
R3725:Vps13d	UTSW	4	145,115,648 (GRCm38)	splice site	probably benign
R3794:Vps13d	UTSW	4	145,085,437 (GRCm38)	splice site	probably benign
R3875:Vps13d	UTSW	4	145,190,544 (GRCm38)	missense	probably damaging	1.00
R3948:Vps13d	UTSW	4	145,141,340 (GRCm38)	missense	probably damaging	1.00
R3953:Vps13d	UTSW	4	145,148,880 (GRCm38)	missense	probably damaging	1.00
R4021:Vps13d	UTSW	4	145,075,061 (GRCm38)	missense	possibly damaging	0.90
R4323:Vps13d	UTSW	4	145,152,778 (GRCm38)	missense	probably benign	0.28
R4346:Vps13d	UTSW	4	145,072,529 (GRCm38)	intron	probably benign
R4509:Vps13d	UTSW	4	145,062,602 (GRCm38)	missense	probably damaging	1.00
R4613:Vps13d	UTSW	4	145,131,655 (GRCm38)	missense	possibly damaging	0.95
R4657:Vps13d	UTSW	4	145,074,842 (GRCm38)	missense	probably damaging	1.00
R4680:Vps13d	UTSW	4	145,108,510 (GRCm38)	missense	possibly damaging	0.94
R4688:Vps13d	UTSW	4	145,178,212 (GRCm38)	missense	probably benign
R4797:Vps13d	UTSW	4	145,054,155 (GRCm38)	missense	probably damaging	1.00
R4798:Vps13d	UTSW	4	145,178,056 (GRCm38)	missense	probably damaging	0.98
R4817:Vps13d	UTSW	4	145,069,165 (GRCm38)	missense	probably damaging	1.00
R4839:Vps13d	UTSW	4	145,085,430 (GRCm38)	missense	possibly damaging	0.95
R4860:Vps13d	UTSW	4	145,087,161 (GRCm38)	missense	probably benign
R4869:Vps13d	UTSW	4	145,128,042 (GRCm38)	missense	probably damaging	1.00
R4904:Vps13d	UTSW	4	145,155,445 (GRCm38)	missense	probably damaging	1.00
R4912:Vps13d	UTSW	4	145,155,857 (GRCm38)	missense	probably benign
R4916:Vps13d	UTSW	4	144,983,393 (GRCm38)	missense	probably damaging	1.00
R4976:Vps13d	UTSW	4	145,105,898 (GRCm38)	missense	possibly damaging	0.82
R5029:Vps13d	UTSW	4	145,156,282 (GRCm38)	missense	probably benign	0.02
R5049:Vps13d	UTSW	4	145,086,766 (GRCm38)	missense	probably damaging	1.00
R5077:Vps13d	UTSW	4	145,088,241 (GRCm38)	missense	probably damaging	0.98
R5119:Vps13d	UTSW	4	145,105,898 (GRCm38)	missense	possibly damaging	0.82
R5227:Vps13d	UTSW	4	145,181,207 (GRCm38)	splice site	probably null
R5291:Vps13d	UTSW	4	145,062,569 (GRCm38)	missense	probably damaging	0.99
R5344:Vps13d	UTSW	4	145,178,334 (GRCm38)	missense	probably damaging	0.98
R5348:Vps13d	UTSW	4	145,065,889 (GRCm38)	missense	probably damaging	0.99
R5478:Vps13d	UTSW	4	145,167,550 (GRCm38)	missense	probably damaging	0.99
R5632:Vps13d	UTSW	4	145,074,882 (GRCm38)	missense	probably damaging	0.99
R5642:Vps13d	UTSW	4	145,170,302 (GRCm38)	missense	possibly damaging	0.66
R5712:Vps13d	UTSW	4	145,087,173 (GRCm38)	missense	probably benign	0.07
R5747:Vps13d	UTSW	4	145,168,283 (GRCm38)	missense	probably benign	0.00
R5752:Vps13d	UTSW	4	145,148,970 (GRCm38)	missense	probably benign	0.06
R5804:Vps13d	UTSW	4	145,100,070 (GRCm38)	missense	probably benign	0.03
R5917:Vps13d	UTSW	4	145,100,010 (GRCm38)	missense	probably damaging	0.96
R5932:Vps13d	UTSW	4	145,045,041 (GRCm38)	missense	possibly damaging	0.71
R5940:Vps13d	UTSW	4	145,074,975 (GRCm38)	missense	probably benign	0.09
R5978:Vps13d	UTSW	4	145,122,611 (GRCm38)	missense	probably benign
R6031:Vps13d	UTSW	4	145,168,509 (GRCm38)	missense	probably benign	0.01
R6031:Vps13d	UTSW	4	145,168,509 (GRCm38)	missense	probably benign	0.01
R6143:Vps13d	UTSW	4	145,148,565 (GRCm38)	missense	possibly damaging	0.95
R6174:Vps13d	UTSW	4	144,975,193 (GRCm38)	nonsense	probably null
R6191:Vps13d	UTSW	4	145,149,348 (GRCm38)	missense	probably damaging	1.00
R6198:Vps13d	UTSW	4	145,148,990 (GRCm38)	missense	probably benign	0.28
R6374:Vps13d	UTSW	4	145,122,681 (GRCm38)	missense	probably damaging	1.00
R6379:Vps13d	UTSW	4	145,088,258 (GRCm38)	missense	probably benign
R6388:Vps13d	UTSW	4	145,155,574 (GRCm38)	missense	probably benign	0.06
R6418:Vps13d	UTSW	4	145,092,280 (GRCm38)	missense	probably damaging	0.98
R6466:Vps13d	UTSW	4	145,057,495 (GRCm38)	missense	possibly damaging	0.47
R6602:Vps13d	UTSW	4	145,103,664 (GRCm38)	intron	probably benign
R6604:Vps13d	UTSW	4	145,181,124 (GRCm38)	missense	probably damaging	1.00
R7051:Vps13d	UTSW	4	145,163,344 (GRCm38)	missense	probably benign	0.00
R7052:Vps13d	UTSW	4	145,163,344 (GRCm38)	missense	probably benign	0.00
R7103:Vps13d	UTSW	4	145,115,492 (GRCm38)	missense
R7231:Vps13d	UTSW	4	145,057,462 (GRCm38)	missense
R7246:Vps13d	UTSW	4	145,156,050 (GRCm38)	missense
R7339:Vps13d	UTSW	4	145,121,368 (GRCm38)	missense
R7409:Vps13d	UTSW	4	145,141,254 (GRCm38)	missense
R7419:Vps13d	UTSW	4	145,115,503 (GRCm38)	missense
R7424:Vps13d	UTSW	4	145,148,747 (GRCm38)	missense
R7439:Vps13d	UTSW	4	145,105,856 (GRCm38)	missense
R7440:Vps13d	UTSW	4	145,128,411 (GRCm38)	missense
R7528:Vps13d	UTSW	4	145,091,922 (GRCm38)	missense
R7547:Vps13d	UTSW	4	145,057,538 (GRCm38)	missense
R7558:Vps13d	UTSW	4	145,154,580 (GRCm38)	missense
R7699:Vps13d	UTSW	4	145,085,405 (GRCm38)	missense
R7729:Vps13d	UTSW	4	145,075,052 (GRCm38)	missense
R7789:Vps13d	UTSW	4	145,100,065 (GRCm38)	missense
R7813:Vps13d	UTSW	4	145,178,063 (GRCm38)	nonsense	probably null
R7834:Vps13d	UTSW	4	145,108,573 (GRCm38)	missense
R7840:Vps13d	UTSW	4	145,103,676 (GRCm38)	missense
R7880:Vps13d	UTSW	4	145,181,114 (GRCm38)	critical splice donor site	probably null
R7912:Vps13d	UTSW	4	145,173,127 (GRCm38)	missense
R7915:Vps13d	UTSW	4	145,086,819 (GRCm38)	missense
R7931:Vps13d	UTSW	4	145,096,284 (GRCm38)	nonsense	probably null
R8021:Vps13d	UTSW	4	145,148,675 (GRCm38)	missense
R8048:Vps13d	UTSW	4	145,155,567 (GRCm38)	missense
R8057:Vps13d	UTSW	4	144,975,183 (GRCm38)	missense
R8063:Vps13d	UTSW	4	145,114,757 (GRCm38)	missense
R8131:Vps13d	UTSW	4	145,156,137 (GRCm38)	missense
R8190:Vps13d	UTSW	4	145,152,751 (GRCm38)	missense
R8226:Vps13d	UTSW	4	145,149,290 (GRCm38)	missense
R8241:Vps13d	UTSW	4	145,148,477 (GRCm38)	missense
R8254:Vps13d	UTSW	4	144,983,312 (GRCm38)	splice site	probably benign
R8305:Vps13d	UTSW	4	145,092,288 (GRCm38)	missense
R8415:Vps13d	UTSW	4	145,091,979 (GRCm38)	missense
R8460:Vps13d	UTSW	4	145,170,439 (GRCm38)	intron	probably benign
R8487:Vps13d	UTSW	4	145,155,247 (GRCm38)	missense	probably benign	0.11
R8543:Vps13d	UTSW	4	145,016,783 (GRCm38)	nonsense	probably null
R8679:Vps13d	UTSW	4	145,085,407 (GRCm38)	missense
R8716:Vps13d	UTSW	4	145,075,778 (GRCm38)	missense
R8749:Vps13d	UTSW	4	145,138,613 (GRCm38)	missense
R8772:Vps13d	UTSW	4	145,075,032 (GRCm38)	missense
R8788:Vps13d	UTSW	4	145,086,735 (GRCm38)	missense
R8789:Vps13d	UTSW	4	145,069,173 (GRCm38)	missense
R8836:Vps13d	UTSW	4	145,156,078 (GRCm38)	missense
R8874:Vps13d	UTSW	4	145,155,202 (GRCm38)	missense
R8918:Vps13d	UTSW	4	145,046,303 (GRCm38)	missense
R9129:Vps13d	UTSW	4	145,171,679 (GRCm38)	missense
R9220:Vps13d	UTSW	4	145,056,488 (GRCm38)	missense
R9233:Vps13d	UTSW	4	145,152,774 (GRCm38)	missense
R9234:Vps13d	UTSW	4	145,149,222 (GRCm38)	missense
R9256:Vps13d	UTSW	4	145,155,804 (GRCm38)	missense
R9350:Vps13d	UTSW	4	145,155,763 (GRCm38)	missense
R9398:Vps13d	UTSW	4	145,170,386 (GRCm38)	nonsense	probably null
R9415:Vps13d	UTSW	4	145,069,957 (GRCm38)	missense
R9438:Vps13d	UTSW	4	145,131,744 (GRCm38)	missense
R9469:Vps13d	UTSW	4	145,054,121 (GRCm38)	missense
R9487:Vps13d	UTSW	4	145,081,299 (GRCm38)	critical splice donor site	probably null
R9524:Vps13d	UTSW	4	145,096,244 (GRCm38)	missense
R9616:Vps13d	UTSW	4	145,098,131 (GRCm38)	missense
R9655:Vps13d	UTSW	4	145,086,735 (GRCm38)	missense
R9709:Vps13d	UTSW	4	145,149,345 (GRCm38)	missense
R9767:Vps13d	UTSW	4	145,152,736 (GRCm38)	missense
R9773:Vps13d	UTSW	4	145,092,049 (GRCm38)	missense
R9779:Vps13d	UTSW	4	145,072,402 (GRCm38)	missense
R9796:Vps13d	UTSW	4	145,127,935 (GRCm38)	critical splice donor site	probably null
X0021:Vps13d	UTSW	4	145,155,025 (GRCm38)	missense	probably damaging	0.99
Z1176:Vps13d	UTSW	4	145,107,067 (GRCm38)	missense
Z1177:Vps13d	UTSW	4	145,178,296 (GRCm38)	missense
Z1177:Vps13d	UTSW	4	145,154,908 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- GCCCAACCCACTGTTTGAAG -3'
(R):5'- TCCAGAAAAGGCAATGTCTTGTACC -3'

Sequencing Primer
(F):5'- CCTAATGAGCAGCTTGACAATGGTC -3'
(R):5'- TACCTTTAGGGGTGTCACTGGAAAAC -3'

Posted On

2016-03-01