Mutagenetix > Incidental Mutation

Incidental Mutation 'R5397:Mplkipl1'

429776

Institutional Source

Beutler Lab

Gene Symbol

Mplkipl1

Ensembl Gene

ENSMUSG00000094649

Gene Name

M-phase specific PLK1 intereacting protein like 1

Synonyms

Gm7102

MMRRC Submission

042968-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.141) question?

Stock #

R5397 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

61163124-61164747 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 61164364 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 24 (G24R)

Ref Sequence

ENSEMBL: ENSMUSP00000137574 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000180168]

AlphaFold

J3QQ10

Predicted Effect

unknown
Transcript: ENSMUST00000180168
AA Change: G24R

SMART Domains

Protein: ENSMUSP00000137574
Gene: ENSMUSG00000094649
AA Change: G24R

Domain	Start	End	E-Value	Type
Pfam:MPLKIP	31	170	8.9e-27	PFAM

Meta Mutation Damage Score

0.1094

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox2	T	C	14: 8,243,803 (GRCm38)	T518A	probably benign	Het
Acvr1b	T	A	15: 101,096,845 (GRCm39)	V254D	probably damaging	Het
Adar	T	C	3: 89,642,626 (GRCm39)	I169T	probably benign	Het
Afg3l2	G	T	18: 67,554,329 (GRCm39)	L458M	probably damaging	Het
Arap1	A	T	7: 101,034,119 (GRCm39)	Q187L	possibly damaging	Het
Atad5	T	A	11: 80,002,319 (GRCm39)	M1037K	probably damaging	Het
Bsg	T	A	10: 79,544,629 (GRCm39)	W56R	probably damaging	Het
C1qtnf3	A	G	15: 10,978,627 (GRCm39)	T276A	probably damaging	Het
Capn2	A	G	1: 182,298,271 (GRCm39)	C665R	probably damaging	Het
Cast	A	G	13: 74,869,056 (GRCm39)	S248P	possibly damaging	Het
Cd68	C	T	11: 69,556,484 (GRCm39)	V108I	probably benign	Het
Cyp2d11	A	T	15: 82,276,279 (GRCm39)	W131R	probably damaging	Het
Dhx58	A	G	11: 100,594,746 (GRCm39)	V50A	probably damaging	Het
Fam124a	A	G	14: 62,843,838 (GRCm39)	S449G	probably benign	Het
Flnc	G	A	6: 29,441,160 (GRCm39)	M371I	possibly damaging	Het
Gad1-ps	G	A	10: 99,281,009 (GRCm39)		noncoding transcript	Het
Gm4787	G	C	12: 81,424,604 (GRCm39)	T518S	probably benign	Het
Gpr149	A	G	3: 62,438,226 (GRCm39)	S644P	probably damaging	Het
Gucy1b1	G	A	3: 81,951,458 (GRCm39)	T274I	possibly damaging	Het
Kcnq5	A	G	1: 21,476,080 (GRCm39)	V541A	probably damaging	Het
Kdm5b	G	A	1: 134,549,836 (GRCm39)		probably null	Het
Lig4	G	T	8: 10,022,644 (GRCm39)	R379S	probably benign	Het
Map7	G	A	10: 20,149,067 (GRCm39)	R514Q	unknown	Het
Mertk	T	A	2: 128,613,384 (GRCm39)	F467I	possibly damaging	Het
Mettl4	A	T	17: 95,034,705 (GRCm39)	Y463*	probably null	Het
Nme8	T	C	13: 19,878,549 (GRCm39)	D70G	probably damaging	Het
Npat	A	G	9: 53,481,774 (GRCm39)	N1161D	probably damaging	Het
Or4k77	T	A	2: 111,199,285 (GRCm39)	C103S	probably benign	Het
Or51ac3	T	A	7: 103,213,713 (GRCm39)	I258F	probably damaging	Het
Or6c76b	T	C	10: 129,692,579 (GRCm39)	F64S	probably damaging	Het
Paxip1	A	T	5: 27,977,002 (GRCm39)		probably benign	Het
Peg10	C	CTCG	6: 4,756,453 (GRCm39)		probably benign	Het
Plxnc1	T	C	10: 94,679,614 (GRCm39)	T923A	probably benign	Het
Pms1	T	A	1: 53,231,279 (GRCm39)	K857*	probably null	Het
Ppp1r9b	A	G	11: 94,892,936 (GRCm39)	E260G	probably damaging	Het
Prpf3	A	T	3: 95,760,891 (GRCm39)	S4T	probably benign	Het
Rdh14	T	A	12: 10,444,869 (GRCm39)	V240D	probably damaging	Het
Ripply1	TTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCT	TTCCTCCTCCTCCTCCTCCTCCTCCTCCT	X: 138,680,599 (GRCm39)		probably benign	Het
S100a1	A	T	3: 90,419,442 (GRCm39)	M1K	probably null	Het
Slc2a5	G	A	4: 150,224,280 (GRCm39)		probably null	Het
Slc5a5	T	C	8: 71,343,823 (GRCm39)	T160A	probably damaging	Het
Srcap	T	G	7: 127,152,468 (GRCm39)		probably null	Het
Tgm6	T	A	2: 129,983,828 (GRCm39)	M329K	possibly damaging	Het
Tom1l1	G	A	11: 90,552,600 (GRCm39)	A201V	probably benign	Het
Trgv5	A	C	13: 19,376,728 (GRCm39)	E42D	possibly damaging	Het
Ttc13	T	C	8: 125,402,002 (GRCm39)	T662A	possibly damaging	Het
Ttn	T	C	2: 76,555,599 (GRCm39)	T30469A	probably damaging	Het
Ube3a	T	A	7: 58,936,660 (GRCm39)	S645R	probably benign	Het
Vgll2	A	G	10: 51,901,262 (GRCm39)	E64G	probably damaging	Het
Vmn1r25	A	T	6: 57,956,060 (GRCm39)	C76*	probably null	Het
Vmn2r101	A	G	17: 19,809,104 (GRCm39)	N78D	probably damaging	Het
Zcchc10	CCAGCAGCAGCAGCAGCAGCAG	CCAGCAGCAGCAGCAGCAG	11: 53,223,344 (GRCm39)		probably benign	Het
Zcchc7	C	A	4: 44,926,048 (GRCm39)	A28E	probably damaging	Het

Other mutations in Mplkipl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01718:Mplkipl1	APN	19	61,164,199 (GRCm39)	missense	probably damaging	0.98
IGL02958:Mplkipl1	APN	19	61,164,118 (GRCm39)	missense	possibly damaging	0.83
R0427:Mplkipl1	UTSW	19	61,163,908 (GRCm39)	missense	probably damaging	1.00
R1886:Mplkipl1	UTSW	19	61,164,136 (GRCm39)	missense	probably damaging	0.97
R4576:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4578:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4615:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4616:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4617:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4621:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4622:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4623:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4826:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4827:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4829:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4830:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4870:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4871:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R4951:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5112:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5301:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5317:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5335:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5399:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5591:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5592:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5594:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5616:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5884:Mplkipl1	UTSW	19	61,164,364 (GRCm39)	missense	unknown
R5919:Mplkipl1	UTSW	19	61,163,907 (GRCm39)	missense	probably damaging	1.00
R7003:Mplkipl1	UTSW	19	61,164,319 (GRCm39)	missense	possibly damaging	0.93
R7112:Mplkipl1	UTSW	19	61,163,997 (GRCm39)	missense	probably damaging	1.00
R7266:Mplkipl1	UTSW	19	61,163,973 (GRCm39)	missense	possibly damaging	0.84
R8489:Mplkipl1	UTSW	19	61,164,085 (GRCm39)	missense	probably damaging	0.98
R8979:Mplkipl1	UTSW	19	61,164,169 (GRCm39)	missense	probably damaging	1.00
Z1177:Mplkipl1	UTSW	19	61,164,188 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- AGTAGCCGAACTGATGCTGG -3'
(R):5'- CAGTTGATCGCTGAGGTTCG -3'

Sequencing Primer
(F):5'- AACTGATGCTGGGACCCC -3'
(R):5'- AGGTTCGGCAGTTGAGGC -3'

Posted On

2016-09-06