Mutagenetix > Incidental Mutation

Incidental Mutation 'R6416:Anxa11'

517957

Institutional Source

Beutler Lab

Gene Symbol

Anxa11

Ensembl Gene

ENSMUSG00000021866

Gene Name

annexin A11

Synonyms

A830099O17Rik, Anx11

MMRRC Submission

044558-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R6416 (G1)

Quality Score

130.008

Status

Validated

Chromosome

Chromosomal Location

25842156-25886804 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 25874270 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Arginine at position 235 (Q235R)

Ref Sequence

ENSEMBL: ENSMUSP00000107983 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022416] [ENSMUST00000112364]

AlphaFold

P97384

Predicted Effect

possibly damaging
Transcript: ENSMUST00000022416
AA Change: Q235R

PolyPhen 2 Score 0.666 (Sensitivity: 0.86; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000022416
Gene: ENSMUSG00000021866
AA Change: Q235R

Domain	Start	End	E-Value	Type
low complexity region	3	31	N/A	INTRINSIC
low complexity region	73	175	N/A	INTRINSIC
ANX	215	267	7.18e-25	SMART
ANX	287	339	7.57e-24	SMART
ANX	371	423	1.35e-20	SMART
ANX	446	498	1.89e-24	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112364
AA Change: Q235R

PolyPhen 2 Score 0.738 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000107983
Gene: ENSMUSG00000021866
AA Change: Q235R

Domain	Start	End	E-Value	Type
low complexity region	3	31	N/A	INTRINSIC
low complexity region	73	175	N/A	INTRINSIC
ANX	215	267	7.18e-25	SMART
ANX	287	339	7.57e-24	SMART
Pfam:Annexin	357	392	1.2e-9	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000124704

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133547

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184083

Coding Region Coverage

1x: 100.0%
3x: 99.8%
10x: 98.4%
20x: 94.6%

Validation Efficiency

99% (67/68)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. [provided by RefSeq, Dec 2015]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310061N02Rik	C	A	16: 88,707,891 (GRCm38)	R6L	unknown	Het
Ap2b1	T	A	11: 83,308,239 (GRCm38)	M1K	probably null	Het
Atl2	G	A	17: 79,850,223 (GRCm38)	T563I	probably benign	Het
Azin1	A	T	15: 38,492,343 (GRCm38)	S307R	possibly damaging	Het
Ccdc153	A	G	9: 44,245,780 (GRCm38)	T118A	probably benign	Het
Chac1	G	A	2: 119,353,534 (GRCm38)	V206I	probably damaging	Het
Chrm3	A	G	13: 9,877,662 (GRCm38)	V446A	probably benign	Het
Cped1	T	C	6: 22,123,649 (GRCm38)	F467S	probably damaging	Het
Csmd3	G	A	15: 48,673,560 (GRCm38)	P82L	probably damaging	Het
Ddx60	T	A	8: 61,977,950 (GRCm38)	S840T	probably benign	Het
Ddx60	C	T	8: 61,998,681 (GRCm38)	H1202Y	probably benign	Het
Dnah8	T	A	17: 30,765,635 (GRCm38)	N3102K	probably benign	Het
Dst	A	T	1: 34,116,128 (GRCm38)	K85M	probably damaging	Het
Ehbp1l1	T	C	19: 5,718,757 (GRCm38)	I839M	probably benign	Het
Fam184b	T	A	5: 45,537,653 (GRCm38)	M750L	probably benign	Het
Frem2	T	C	3: 53,572,378 (GRCm38)	T1965A	probably benign	Het
Ftl1	A	T	7: 45,459,210 (GRCm38)	D41E	probably benign	Het
Garem2	G	A	5: 30,116,737 (GRCm38)	W698*	probably null	Het
Glt8d2	T	C	10: 82,652,906 (GRCm38)	Y283C	probably damaging	Het
Hars	T	C	18: 36,773,590 (GRCm38)	E109G	possibly damaging	Het
Hspb9	T	C	11: 100,714,210 (GRCm38)	S121P	probably damaging	Het
Hus1b	A	T	13: 30,947,205 (GRCm38)	L157Q	probably damaging	Het
Hykk	A	G	9: 54,946,359 (GRCm38)	M322V	probably benign	Het
Igkv4-61	C	A	6: 69,417,154 (GRCm38)	A31S	possibly damaging	Het
Il17f	T	A	1: 20,777,907 (GRCm38)	M116L	probably benign	Het
Kif22	T	C	7: 127,028,932 (GRCm38)	K9E	possibly damaging	Het
Krt90	T	C	15: 101,559,244 (GRCm38)	E233G	probably benign	Het
Lipg	T	C	18: 74,957,236 (GRCm38)	M81V	probably benign	Het
Mocos	C	T	18: 24,701,456 (GRCm38)	S850L	probably damaging	Het
Mug2	T	A	6: 122,082,754 (GRCm38)	S1364T	probably damaging	Het
Neb	A	T	2: 52,185,328 (GRCm38)	N208K	probably benign	Het
Oca2	G	T	7: 56,328,767 (GRCm38)	R561L	probably benign	Het
Olfm5	A	G	7: 104,154,053 (GRCm38)	L401P	probably damaging	Het
Olfr109	T	A	17: 37,467,080 (GRCm38)	Y291*	probably null	Het
Olfr1238	C	A	2: 89,406,522 (GRCm38)	A186S	possibly damaging	Het
Olfr331	T	C	11: 58,502,340 (GRCm38)	D72G	probably damaging	Het
Olfr828	G	A	9: 18,815,892 (GRCm38)	T134M	probably benign	Het
Olfr952	T	A	9: 39,426,891 (GRCm38)	Y60F	probably damaging	Het
Oxgr1	T	C	14: 120,022,448 (GRCm38)	N116D	probably damaging	Het
Pcdha11	G	T	18: 37,012,169 (GRCm38)		probably null	Het
Pi4ka	T	C	16: 17,358,322 (GRCm38)	I418V	probably benign	Het
Pih1d2	T	C	9: 50,618,609 (GRCm38)	V62A	probably benign	Het
Pkib	T	A	10: 57,728,138 (GRCm38)	V46E	probably damaging	Het
Pum1	A	G	4: 130,728,287 (GRCm38)		probably null	Het
Rbak	G	T	5: 143,176,552 (GRCm38)	Q19K	possibly damaging	Het
Sema3c	A	G	5: 17,576,961 (GRCm38)	T32A	probably damaging	Het
Shprh	T	C	10: 11,167,873 (GRCm38)	W835R	probably damaging	Het
Sis	T	G	3: 72,911,854 (GRCm38)	K1456N	probably damaging	Het
Slc4a4	A	T	5: 89,179,729 (GRCm38)	N675I	probably benign	Het
Slc8a3	C	A	12: 81,315,627 (GRCm38)	M139I	probably damaging	Het
Sorcs3	T	C	19: 48,802,759 (GRCm38)	F1182S	probably damaging	Het
St8sia2	A	T	7: 73,971,921 (GRCm38)	I96N	probably damaging	Het
Stx19	T	C	16: 62,822,057 (GRCm38)	S79P	probably damaging	Het
Tecta	C	T	9: 42,375,267 (GRCm38)	V698M	probably damaging	Het
Timm22	T	C	11: 76,411,139 (GRCm38)	S150P	probably damaging	Het
Tjp1	C	T	7: 65,313,205 (GRCm38)	D995N	possibly damaging	Het
Tmem135	T	A	7: 89,147,794 (GRCm38)	T365S	probably benign	Het
Tmem174	T	C	13: 98,636,981 (GRCm38)	T114A	probably benign	Het
Tnc	A	G	4: 64,007,816 (GRCm38)	I909T	probably benign	Het
Trcg1	A	G	9: 57,241,330 (GRCm38)	I62V	possibly damaging	Het
Tulp1	A	T	17: 28,356,031 (GRCm38)	*487K	probably null	Het
Unc79	T	A	12: 103,131,646 (GRCm38)	V1826E	possibly damaging	Het
Vmn2r13	A	T	5: 109,174,116 (GRCm38)	N238K	probably damaging	Het
Vmn2r22	T	A	6: 123,637,738 (GRCm38)	N298Y	probably damaging	Het
Vmn2r23	T	A	6: 123,712,902 (GRCm38)	F246I	probably damaging	Het
Vps9d1	A	C	8: 123,248,639 (GRCm38)	V194G	probably damaging	Het
Zfp260	A	T	7: 30,104,810 (GRCm38)	H45L	possibly damaging	Het
Zfp846	A	T	9: 20,593,720 (GRCm38)	H292L	possibly damaging	Het

Other mutations in Anxa11

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02194:Anxa11	APN	14	25,870,129 (GRCm38)	missense	unknown
twirl	UTSW	14	25,872,734 (GRCm38)	missense	unknown
R0597:Anxa11	UTSW	14	25,874,228 (GRCm38)	missense	probably damaging	1.00
R0656:Anxa11	UTSW	14	25,873,997 (GRCm38)	missense	probably damaging	1.00
R0717:Anxa11	UTSW	14	25,874,789 (GRCm38)	splice site	probably null
R1087:Anxa11	UTSW	14	25,870,179 (GRCm38)	missense	unknown
R2207:Anxa11	UTSW	14	25,874,297 (GRCm38)	missense	probably damaging	1.00
R5041:Anxa11	UTSW	14	25,874,764 (GRCm38)	nonsense	probably null
R6298:Anxa11	UTSW	14	25,872,734 (GRCm38)	missense	unknown
R6944:Anxa11	UTSW	14	25,874,752 (GRCm38)	missense	probably damaging	0.99
R7389:Anxa11	UTSW	14	25,872,888 (GRCm38)	missense	probably damaging	0.99
R7760:Anxa11	UTSW	14	25,872,827 (GRCm38)	nonsense	probably null
R8881:Anxa11	UTSW	14	25,874,263 (GRCm38)	missense	probably damaging	1.00
X0005:Anxa11	UTSW	14	25,874,290 (GRCm38)	missense	probably benign	0.03
Z1177:Anxa11	UTSW	14	25,870,176 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GGTAAGAGAGCAGACTGACTCC -3'
(R):5'- AGGCTTATCTTGGCAAACCTAC -3'

Sequencing Primer
(F):5'- TGCAGGTCACTGTGTAGGCC -3'
(R):5'- TACCCTAACTAAGCTCATGGTCATC -3'

Posted On

2018-05-24