Incidental Mutation 'R8029:Ces2b'
ID 617830
Institutional Source Beutler Lab
Gene Symbol Ces2b
Ensembl Gene ENSMUSG00000050097
Gene Name carboxyesterase 2B
Synonyms
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.048) question?
Stock # R8029 (G1)
Quality Score 225.009
Status Not validated
Chromosome 8
Chromosomal Location 104831572-104840093 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 104834850 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Asparagine to Serine at position 192 (N192S)
Ref Sequence ENSEMBL: ENSMUSP00000063005 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000059449] [ENSMUST00000163042]
AlphaFold Q6PDB7
Predicted Effect probably damaging
Transcript: ENSMUST00000059449
AA Change: N192S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000063005
Gene: ENSMUSG00000050097
AA Change: N192S

DomainStartEndE-ValueType
Pfam:COesterase 10 535 8.5e-175 PFAM
Pfam:Abhydrolase_3 140 305 1.8e-11 PFAM
Pfam:Peptidase_S9 161 296 9e-8 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000163042
SMART Domains Protein: ENSMUSP00000124796
Gene: ENSMUSG00000050097

DomainStartEndE-ValueType
signal peptide 1 26 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 71 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700014D04Rik T C 13: 59,742,377 H543R possibly damaging Het
Acvr1c A G 2: 58,296,117 I118T possibly damaging Het
Alpk1 T C 3: 127,729,285 D36G possibly damaging Het
Aox2 G A 1: 58,343,668 V1036I probably benign Het
Arhgef1 T C 7: 24,919,738 L468P probably damaging Het
Armc2 A T 10: 41,927,000 L559Q probably damaging Het
Asb3 A T 11: 31,101,180 R506* probably null Het
Asna1 T C 8: 85,019,827 M131V probably benign Het
Aspm A G 1: 139,471,632 H1045R probably benign Het
Cacna1g G T 11: 94,409,738 R2099S probably benign Het
Cd177 C T 7: 24,756,169 W309* probably null Het
Cd34 A C 1: 194,958,552 M242L probably benign Het
Cdh24 T A 14: 54,639,399 N49I probably damaging Het
Chsy3 A G 18: 59,179,447 I331V possibly damaging Het
Disp1 A G 1: 183,089,288 S523P probably damaging Het
Dlgap5 T C 14: 47,416,440 H44R probably benign Het
Dnajc30 G T 5: 135,064,332 A28S probably benign Het
Dsc2 C T 18: 20,032,274 G881R possibly damaging Het
Exd1 A T 2: 119,528,723 H226Q probably damaging Het
Gata6 T A 18: 11,054,944 V291E possibly damaging Het
Gfi1b A T 2: 28,613,675 probably null Het
Grk3 T A 5: 112,961,642 I150L probably benign Het
Gstcd C T 3: 133,082,107 V277M probably damaging Het
Idua T C 5: 108,669,412 F17S probably benign Het
Ighg1 T C 12: 113,329,145 K268R Het
Lama1 G T 17: 67,817,594 R2883M Het
Lpar3 T C 3: 146,240,963 M132T probably benign Het
Macf1 T C 4: 123,444,892 T4351A possibly damaging Het
March6 A G 15: 31,496,002 probably null Het
Mbtps1 C A 8: 119,547,805 probably benign Het
Mctp1 T C 13: 77,029,886 Y931H probably damaging Het
Mug2 A G 6: 122,081,545 probably null Het
Myh1 G T 11: 67,211,240 probably null Het
Mylk2 T C 2: 152,920,299 S497P probably damaging Het
Nectin4 A G 1: 171,386,687 D470G probably benign Het
Nop2 C T 6: 125,144,420 P722S possibly damaging Het
Nphp1 T C 2: 127,741,116 T626A probably benign Het
Nudt9 C T 5: 104,050,611 probably benign Het
Olfr1145 A G 2: 87,810,032 T71A probably benign Het
Olfr18 A G 9: 20,314,347 F191S possibly damaging Het
Olfr512 T A 7: 108,713,830 F159Y possibly damaging Het
Olfr835 T C 9: 19,035,794 S224P probably damaging Het
Oplah T C 15: 76,305,696 Y143C probably benign Het
Osbpl1a T G 18: 12,914,521 E125D probably benign Het
P2ry1 C A 3: 61,003,522 N27K possibly damaging Het
Palld A T 8: 61,877,312 I177N probably damaging Het
Plcl1 A T 1: 55,696,078 I193L probably benign Het
Polr1a T A 6: 71,912,956 L53* probably null Het
Ppp1r9a A G 6: 5,057,518 E531G possibly damaging Het
Prex1 C A 2: 166,575,603 Q1361H probably benign Het
Ptprc T C 1: 138,078,459 E795G probably damaging Het
Rars C T 11: 35,821,165 V295I probably damaging Het
Rnaseh2b T C 14: 62,353,548 V116A possibly damaging Het
Rnf43 T A 11: 87,731,894 L480H probably benign Het
Rttn A G 18: 89,090,474 T1601A not run Het
Setd1a C G 7: 127,786,214 Q698E probably benign Het
Sh3gl3 T C 7: 82,270,883 Y100H probably benign Het
Sis T C 3: 72,921,142 Y1200C probably damaging Het
Snx13 A C 12: 35,119,886 H610P probably damaging Het
Spdl1 T C 11: 34,822,592 R217G probably benign Het
Spop G A 11: 95,474,367 E113K probably benign Het
Sult2a3 G A 7: 14,121,628 P101L probably damaging Het
Tbl1xr1 C T 3: 22,200,436 H348Y probably damaging Het
Tmie T C 9: 110,867,487 T109A possibly damaging Het
Ttc12 A G 9: 49,470,251 V140A possibly damaging Het
Ube2m A G 7: 13,036,597 L58P probably damaging Het
Urb1 A G 16: 90,779,152 S839P possibly damaging Het
Vmn2r117 T A 17: 23,477,770 D221V probably benign Het
Vps41 C T 13: 18,823,785 Q263* probably null Het
Zfand3 A T 17: 30,135,433 T75S probably benign Het
Zscan26 A G 13: 21,445,350 C202R probably damaging Het
Other mutations in Ces2b
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01388:Ces2b APN 8 104834604 splice site probably benign
IGL01905:Ces2b APN 8 104833962 missense probably damaging 1.00
IGL02528:Ces2b APN 8 104834969 missense probably damaging 1.00
IGL02659:Ces2b APN 8 104832570 splice site probably benign
IGL02885:Ces2b APN 8 104834931 missense probably damaging 1.00
PIT4142001:Ces2b UTSW 8 104836810 missense probably damaging 1.00
R0092:Ces2b UTSW 8 104836512 missense possibly damaging 0.48
R0403:Ces2b UTSW 8 104833945 missense probably damaging 0.98
R0600:Ces2b UTSW 8 104835910 missense probably benign 0.06
R0637:Ces2b UTSW 8 104834605 splice site probably benign
R1574:Ces2b UTSW 8 104835889 missense probably benign 0.16
R1574:Ces2b UTSW 8 104835889 missense probably benign 0.16
R3036:Ces2b UTSW 8 104834626 missense possibly damaging 0.87
R3086:Ces2b UTSW 8 104832769 missense possibly damaging 0.92
R4761:Ces2b UTSW 8 104836561 critical splice donor site probably null
R4920:Ces2b UTSW 8 104836906 missense probably benign
R4937:Ces2b UTSW 8 104832781 missense probably benign 0.29
R5211:Ces2b UTSW 8 104835063 missense possibly damaging 0.89
R5550:Ces2b UTSW 8 104838437 missense probably benign 0.00
R5790:Ces2b UTSW 8 104833936 missense probably damaging 1.00
R6403:Ces2b UTSW 8 104836269 nonsense probably null
R6692:Ces2b UTSW 8 104837287 missense probably damaging 1.00
R6720:Ces2b UTSW 8 104836869 missense probably benign 0.32
R6899:Ces2b UTSW 8 104836766 splice site probably null
R7148:Ces2b UTSW 8 104838296 missense probably damaging 1.00
R7270:Ces2b UTSW 8 104837840 missense possibly damaging 0.50
R7571:Ces2b UTSW 8 104835009 missense probably damaging 1.00
R7626:Ces2b UTSW 8 104837385 missense possibly damaging 0.82
R7841:Ces2b UTSW 8 104835060 missense probably benign 0.19
R7892:Ces2b UTSW 8 104832753 missense probably damaging 1.00
R8293:Ces2b UTSW 8 104832626 missense unknown
R8296:Ces2b UTSW 8 104836480 missense possibly damaging 0.77
R8721:Ces2b UTSW 8 104833895 missense possibly damaging 0.88
R9100:Ces2b UTSW 8 104831589 unclassified probably benign
R9361:Ces2b UTSW 8 104837407 critical splice donor site probably null
R9461:Ces2b UTSW 8 104837379 missense probably benign 0.39
R9477:Ces2b UTSW 8 104833924 missense probably damaging 1.00
X0027:Ces2b UTSW 8 104833928 missense probably damaging 1.00
Z1177:Ces2b UTSW 8 104832595 frame shift probably null
Predicted Primers PCR Primer
(F):5'- ATGGATTCACGGAGGTGGAC -3'
(R):5'- ATGATGGCTCCATGGAAGAGTC -3'

Sequencing Primer
(F):5'- ACGATGGATCTCTATTGGCAGCC -3'
(R):5'- GCTCCATGGAAGAGTCCTTTG -3'
Posted On 2020-01-23