Mutagenetix > Incidental Mutation

Incidental Mutation 'R7762:Fat1'

597939

Institutional Source

Beutler Lab

Gene Symbol

Fat1

Ensembl Gene

ENSMUSG00000070047

Gene Name

FAT atypical cadherin 1

Synonyms

mFat1, Fath, 2310038E12Rik

MMRRC Submission

045818-MU

Accession Numbers

Genbank: NM_001081286

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7762 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

44935447-45052257 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 45037337 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Histidine at position 3762 (L3762H)

Ref Sequence

ENSEMBL: ENSMUSP00000149194 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098796] [ENSMUST00000189017] [ENSMUST00000191428] [ENSMUST00000215588]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000098796
AA Change: L3739H

PolyPhen 2 Score 0.935 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000096394
Gene: ENSMUSG00000070047
AA Change: L3739H

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000189017
AA Change: L3739H

Predicted Effect

possibly damaging
Transcript: ENSMUST00000191428
AA Change: L3739H

PolyPhen 2 Score 0.935 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000140596
Gene: ENSMUSG00000070047
AA Change: L3739H

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000215588
AA Change: L3762H

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

Meta Mutation Damage Score

0.1120

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (79/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit holoprosencephaly, anophthalmia, kidney defects and perinatal lethality. Mice homozygous for a hypomorphic allele exhibit altered shoulder girdle and facial musculature, retinal defects, abnormal inner earpatterning and kidney defects. [provided by MGI curators]

Allele List at MGI

All alleles(56) : Targeted, other(1) Gene trapped(55)

Other mutations in this stock

Total: 82 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600015I10Rik	G	A	6: 48,932,686	V622I	probably benign	Het
9130011E15Rik	A	G	19: 45,940,443		probably null	Het
Acod1	T	G	14: 103,051,340	D95E	probably damaging	Het
Agpat4	A	G	17: 12,210,322	T154A	possibly damaging	Het
Ahnak2	A	T	12: 112,775,680	S653T	probably benign	Het
Aox2	C	T	1: 58,349,104	P1124S	probably damaging	Het
Atp4a	T	C	7: 30,720,036	I637T	probably damaging	Het
Atxn7	T	A	14: 14,100,467	C718S	probably damaging	Het
Btbd2	T	A	10: 80,643,556	I516F	probably damaging	Het
Card6	A	G	15: 5,105,338	S128P	probably benign	Het
Cat	T	C	2: 103,456,858	K476E	probably benign	Het
Ccr9	A	T	9: 123,779,957	T235S	probably benign	Het
Cep55	T	G	19: 38,069,069		probably null	Het
Clec2e	A	G	6: 129,095,128	F96S	possibly damaging	Het
Clk2	G	A	3: 89,167,191	V53I	probably benign	Het
Clnk	T	C	5: 38,768,141	M106V	probably benign	Het
Col6a5	T	C	9: 105,931,324	I842V	unknown	Het
Csf1r	A	T	18: 61,110,500	N196I	probably benign	Het
D5Ertd579e	A	T	5: 36,613,381	N116K		Het
Dnaja4	A	G	9: 54,709,210	I166V	probably benign	Het
Dqx1	A	G	6: 83,061,032	E467G	probably damaging	Het
Dync2h1	T	C	9: 7,129,719	T1760A	probably benign	Het
Eea1	T	C	10: 96,028,439	V940A	probably benign	Het
Egr1	T	A	18: 34,863,545	V460E	probably damaging	Het
Eral1	A	G	11: 78,074,533	I352T	possibly damaging	Het
F2	T	C	2: 91,628,696	H476R	possibly damaging	Het
Fam83b	A	G	9: 76,492,432	V463A	possibly damaging	Het
Fibp	A	T	19: 5,464,174	N296Y	probably benign	Het
Figla	A	G	6: 86,017,326	M28V	probably benign	Het
Foxd3	C	A	4: 99,657,125	Y167*	probably null	Het
Gm17019	T	A	5: 15,030,992	H145L	probably benign	Het
Gm340	T	C	19: 41,583,667	L287S	probably benign	Het
Gm48552	C	T	10: 81,390,435	P18L	probably damaging	Het
Gm9573	G	A	17: 35,622,085	T403I	unknown	Het
H2-Q6	A	G	17: 35,428,101	N283S	probably benign	Het
Hrh2	T	A	13: 54,214,039	C11*	probably null	Het
Hrnr	G	T	3: 93,332,199	G3248V	unknown	Het
Iapp	C	A	6: 142,303,396	N58K	possibly damaging	Het
Itga5	T	C	15: 103,349,757	N837S	probably benign	Het
Klhl35	C	A	7: 99,468,440	H64N	probably benign	Het
Lrba	T	A	3: 86,532,201	V2015E	probably damaging	Het
Mdn1	T	C	4: 32,734,421	I3276T	probably benign	Het
Mlh3	G	T	12: 85,268,284	T376K	possibly damaging	Het
Nbea	A	T	3: 55,649,705	H2550Q	probably damaging	Het
Nid1	G	A	13: 13,489,045	G763D	probably damaging	Het
Ntf5	C	A	7: 45,415,819	A125E	probably damaging	Het
Obsl1	A	T	1: 75,503,523	C460S	probably benign	Het
Olfr1272	T	A	2: 90,296,631	K77*	probably null	Het
Olfr130	G	A	17: 38,067,675	C168Y	probably damaging	Het
Olfr1380	T	A	11: 49,564,761	I280N	possibly damaging	Het
Olfr1496	A	T	19: 13,781,286	R223W	probably damaging	Het
Olfr348	A	T	2: 36,787,010	I162F	probably benign	Het
Olfr820	A	G	10: 130,017,181		probably benign	Het
Olfr933	G	A	9: 38,976,194	V173I	probably benign	Het
Orai3	G	A	7: 127,773,571	G130S	unknown	Het
Pcdhb12	T	G	18: 37,435,924	V41G	probably damaging	Het
Plec	A	T	15: 76,183,623	L1194Q	unknown	Het
Pml	C	A	9: 58,220,173	C763F	probably damaging	Het
Prdm15	T	A	16: 97,818,273	I318F	probably benign	Het
Rcbtb2	C	A	14: 73,178,466	T473N	probably benign	Het
Sbno1	T	A	5: 124,374,666	I1347F	probably benign	Het
Secisbp2l	C	T	2: 125,768,193	D269N	probably damaging	Het
Serpina9	A	T	12: 104,001,316	F273L	probably damaging	Het
Sgms2	T	A	3: 131,323,249	Y319F	probably benign	Het
Sgo2b	A	T	8: 63,926,497	H1100Q	probably benign	Het
Sh3bp5l	A	T	11: 58,345,928		probably null	Het
Shh	T	G	5: 28,466,666	K33T	probably benign	Het
Slc5a9	A	G	4: 111,890,174	Y339H	probably damaging	Het
Spice1	T	A	16: 44,370,501		probably null	Het
Tbx2	A	G	11: 85,835,901	E257G	probably damaging	Het
Tenm2	G	A	11: 36,023,306	T2468I	possibly damaging	Het
Tmeff2	A	T	1: 50,979,416	N186Y	probably benign	Het
Tmem132c	T	C	5: 127,554,696	V673A	possibly damaging	Het
Trappc11	G	T	8: 47,522,376	T269K	probably damaging	Het
Trpc6	T	C	9: 8,653,149	F652S	possibly damaging	Het
Trpv1	G	T	11: 73,254,222	K711N	probably benign	Het
Vcan	G	T	13: 89,692,937	P1496Q	probably damaging	Het
Vmn1r192	G	T	13: 22,187,675	A125E	probably damaging	Het
Vti1b	A	T	12: 79,164,946		probably null	Het
Vwa3b	A	C	1: 37,124,045	D583A	probably damaging	Het
Zfp638	T	C	6: 83,976,272	S1120P	probably damaging	Het
Zfyve26	A	T	12: 79,268,635	F1356I	probably benign	Het

Other mutations in Fat1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Fat1	APN	8	45024602	missense	possibly damaging	0.93
IGL00157:Fat1	APN	8	44951670	missense	possibly damaging	0.96
IGL00481:Fat1	APN	8	45050940	missense	probably benign	0.18
IGL00983:Fat1	APN	8	45033390	missense	probably damaging	1.00
IGL01089:Fat1	APN	8	45017857	missense	probably damaging	1.00
IGL01135:Fat1	APN	8	45024840	missense	probably damaging	1.00
IGL01143:Fat1	APN	8	45035532	missense	possibly damaging	0.72
IGL01155:Fat1	APN	8	45023949	missense	probably damaging	1.00
IGL01376:Fat1	APN	8	45026841	missense	probably benign	0.00
IGL01411:Fat1	APN	8	45026800	missense	probably damaging	1.00
IGL01443:Fat1	APN	8	45040576	missense	probably damaging	1.00
IGL01453:Fat1	APN	8	45051270	missense	probably damaging	1.00
IGL01606:Fat1	APN	8	45023049	missense	probably benign	0.26
IGL01622:Fat1	APN	8	45029555	missense	possibly damaging	0.64
IGL01623:Fat1	APN	8	45029555	missense	possibly damaging	0.64
IGL01672:Fat1	APN	8	45040700	missense	probably benign	0.05
IGL01735:Fat1	APN	8	45036239	missense	probably benign	0.07
IGL01793:Fat1	APN	8	44989112	missense	probably benign
IGL01820:Fat1	APN	8	45010502	missense	probably damaging	1.00
IGL01969:Fat1	APN	8	44952599	missense	probably damaging	0.98
IGL02012:Fat1	APN	8	45027540	missense	possibly damaging	0.95
IGL02227:Fat1	APN	8	45023659	missense	probably damaging	1.00
IGL02256:Fat1	APN	8	44950332	missense	probably damaging	1.00
IGL02273:Fat1	APN	8	44950331	missense	probably damaging	1.00
IGL02317:Fat1	APN	8	45025818	missense	probably benign	0.33
IGL02324:Fat1	APN	8	45040556	missense	probably damaging	1.00
IGL02336:Fat1	APN	8	44951583	missense	probably benign	0.16
IGL02442:Fat1	APN	8	44950323	missense	probably benign	0.02
IGL02486:Fat1	APN	8	45025072	missense	probably benign	0.16
IGL02551:Fat1	APN	8	45051398	missense	probably damaging	1.00
IGL02617:Fat1	APN	8	45035591	missense	probably benign	0.31
IGL02698:Fat1	APN	8	45023164	missense	probably benign
IGL02885:Fat1	APN	8	44989167	missense	probably benign	0.01
IGL02904:Fat1	APN	8	45040682	missense	probably damaging	1.00
IGL02953:Fat1	APN	8	45024314	missense	probably damaging	1.00
IGL03108:Fat1	APN	8	45023614	missense	probably damaging	1.00
IGL03153:Fat1	APN	8	45030123	missense	possibly damaging	0.83
IGL03183:Fat1	APN	8	44950586	missense	probably damaging	0.99
IGL03327:Fat1	APN	8	44950468	missense	probably damaging	1.00
IGL03405:Fat1	APN	8	45025241	missense	probably damaging	1.00
Laggardly	UTSW	8	45044464	missense	probably damaging	1.00
R2257_fat1_465	UTSW	8	44950371	missense	probably damaging	1.00
Shrinkage	UTSW	8	45018037	missense	probably damaging	1.00
F5493:Fat1	UTSW	8	45025480	missense	probably damaging	0.99
G1citation:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
I2289:Fat1	UTSW	8	45024996	missense	probably benign	0.01
IGL02837:Fat1	UTSW	8	45017434	missense	probably benign	0.00
PIT4283001:Fat1	UTSW	8	45029540	missense	probably damaging	1.00
PIT4283001:Fat1	UTSW	8	45037207	missense	probably damaging	1.00
PIT4576001:Fat1	UTSW	8	45024645	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R0078:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R0197:Fat1	UTSW	8	45026553	missense	probably benign	0.00
R0328:Fat1	UTSW	8	45023790	missense	probably benign	0.35
R0367:Fat1	UTSW	8	45024313	missense	probably damaging	1.00
R0371:Fat1	UTSW	8	44951892	missense	probably damaging	1.00
R0380:Fat1	UTSW	8	45010123	missense	probably damaging	0.97
R0389:Fat1	UTSW	8	44950348	missense	probably benign	0.00
R0433:Fat1	UTSW	8	45024649	missense	possibly damaging	0.51
R0456:Fat1	UTSW	8	45029534	missense	probably damaging	1.00
R0494:Fat1	UTSW	8	44950542	missense	probably damaging	1.00
R0506:Fat1	UTSW	8	45022951	missense	probably damaging	0.99
R0512:Fat1	UTSW	8	44951332	nonsense	probably null
R0624:Fat1	UTSW	8	45051168	missense	possibly damaging	0.46
R0701:Fat1	UTSW	8	45026553	missense	probably benign	0.00
R0723:Fat1	UTSW	8	45026749	missense	probably damaging	1.00
R0787:Fat1	UTSW	8	45040555	missense	probably damaging	1.00
R0788:Fat1	UTSW	8	45023983	missense	probably benign	0.27
R0862:Fat1	UTSW	8	45018037	missense	probably damaging	1.00
R0864:Fat1	UTSW	8	45018037	missense	probably damaging	1.00
R0907:Fat1	UTSW	8	45026598	missense	probably benign	0.08
R0962:Fat1	UTSW	8	45033326	splice site	probably benign
R1051:Fat1	UTSW	8	45044506	missense	probably damaging	1.00
R1156:Fat1	UTSW	8	45039890	missense	possibly damaging	0.94
R1237:Fat1	UTSW	8	45044279	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45010545	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45010545	missense	probably damaging	1.00
R1478:Fat1	UTSW	8	45025622	missense	probably damaging	0.99
R1482:Fat1	UTSW	8	44953244	missense	probably benign	0.04
R1496:Fat1	UTSW	8	45033390	missense	probably damaging	1.00
R1498:Fat1	UTSW	8	45025484	nonsense	probably null
R1508:Fat1	UTSW	8	45026862	missense	probably benign	0.01
R1577:Fat1	UTSW	8	45023383	missense	probably benign	0.30
R1646:Fat1	UTSW	8	45018042	missense	probably damaging	1.00
R1652:Fat1	UTSW	8	45025178	nonsense	probably null
R1656:Fat1	UTSW	8	45025530	nonsense	probably null
R1662:Fat1	UTSW	8	44953164	missense	probably benign	0.20
R1672:Fat1	UTSW	8	45036835	missense	probably damaging	1.00
R1704:Fat1	UTSW	8	45025576	missense	probably damaging	1.00
R1708:Fat1	UTSW	8	45024792	missense	probably damaging	1.00
R1710:Fat1	UTSW	8	45010482	missense	probably benign	0.00
R1812:Fat1	UTSW	8	45036803	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	44953304	missense	probably benign	0.01
R1872:Fat1	UTSW	8	45038349	missense	probably damaging	1.00
R1883:Fat1	UTSW	8	45051147	missense	probably benign	0.17
R1893:Fat1	UTSW	8	45023856	missense	probably damaging	1.00
R1930:Fat1	UTSW	8	45044228	missense	possibly damaging	0.91
R1931:Fat1	UTSW	8	45044228	missense	possibly damaging	0.91
R1952:Fat1	UTSW	8	45033926	missense	probably benign	0.00
R1957:Fat1	UTSW	8	45040682	missense	probably damaging	1.00
R1999:Fat1	UTSW	8	44952393	missense	probably damaging	0.96
R2019:Fat1	UTSW	8	45023746	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45024332	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45026704	missense	probably damaging	1.00
R2117:Fat1	UTSW	8	45037463	missense	probably benign	0.33
R2196:Fat1	UTSW	8	45024646	missense	probably damaging	1.00
R2204:Fat1	UTSW	8	45023700	missense	probably damaging	1.00
R2256:Fat1	UTSW	8	44950371	missense	probably damaging	1.00
R2257:Fat1	UTSW	8	44950371	missense	probably damaging	1.00
R2409:Fat1	UTSW	8	45040530	splice site	probably benign
R2416:Fat1	UTSW	8	45026383	missense	probably damaging	1.00
R3021:Fat1	UTSW	8	45044011	missense	probably damaging	1.00
R3108:Fat1	UTSW	8	45045173	splice site	probably null
R3109:Fat1	UTSW	8	45045173	splice site	probably null
R3196:Fat1	UTSW	8	44951868	missense	probably benign	0.00
R3683:Fat1	UTSW	8	45017938	missense	probably benign
R3732:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3732:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3733:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3753:Fat1	UTSW	8	45025479	missense	probably damaging	0.97
R3905:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R3907:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R3908:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R4060:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4061:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4062:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4063:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4078:Fat1	UTSW	8	44989122	missense	probably damaging	0.99
R4105:Fat1	UTSW	8	45036851	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45010437	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45050944	missense	probably damaging	1.00
R4161:Fat1	UTSW	8	45036787	missense	probably benign	0.00
R4364:Fat1	UTSW	8	44952962	missense	probably benign	0.01
R4394:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4395:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4396:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4412:Fat1	UTSW	8	45023599	missense	probably damaging	0.99
R4542:Fat1	UTSW	8	45041894	missense	probably damaging	1.00
R4591:Fat1	UTSW	8	45026242	missense	probably benign
R4606:Fat1	UTSW	8	44950683	missense	possibly damaging	0.47
R4612:Fat1	UTSW	8	45025147	missense	probably damaging	1.00
R4730:Fat1	UTSW	8	45033477	missense	probably damaging	1.00
R4778:Fat1	UTSW	8	45038326	missense	probably benign	0.04
R4824:Fat1	UTSW	8	44989114	missense	probably damaging	1.00
R4829:Fat1	UTSW	8	45036162	missense	probably damaging	1.00
R4832:Fat1	UTSW	8	45013065	missense	possibly damaging	0.95
R4849:Fat1	UTSW	8	45012970	missense	probably benign	0.15
R4896:Fat1	UTSW	8	44951280	missense	possibly damaging	0.68
R4927:Fat1	UTSW	8	45022963	missense	probably damaging	0.96
R4941:Fat1	UTSW	8	45036275	missense	probably benign	0.00
R5011:Fat1	UTSW	8	45031263	critical splice acceptor site	probably null
R5040:Fat1	UTSW	8	45023380	missense	probably damaging	1.00
R5112:Fat1	UTSW	8	45024282	missense	probably damaging	1.00
R5151:Fat1	UTSW	8	44951814	missense	possibly damaging	0.74
R5161:Fat1	UTSW	8	44952512	missense	probably benign	0.00
R5162:Fat1	UTSW	8	45025809	missense	probably benign	0.02
R5353:Fat1	UTSW	8	45036131	missense	probably benign	0.13
R5425:Fat1	UTSW	8	45025885	missense	possibly damaging	0.64
R5458:Fat1	UTSW	8	45013053	missense	probably damaging	1.00
R5479:Fat1	UTSW	8	45036875	missense	possibly damaging	0.88
R5543:Fat1	UTSW	8	45023479	missense	probably damaging	0.99
R5569:Fat1	UTSW	8	45039836	missense	probably damaging	0.98
R5610:Fat1	UTSW	8	44953072	nonsense	probably null
R5734:Fat1	UTSW	8	45051209	missense	probably damaging	0.99
R5832:Fat1	UTSW	8	45017423	missense	possibly damaging	0.65
R5860:Fat1	UTSW	8	45051129	missense	probably benign
R5886:Fat1	UTSW	8	45027681	critical splice donor site	probably null
R5886:Fat1	UTSW	8	45033395	missense	probably damaging	1.00
R5919:Fat1	UTSW	8	45026873	critical splice donor site	probably null
R5930:Fat1	UTSW	8	45044036	missense	probably benign	0.10
R5960:Fat1	UTSW	8	45033368	missense	probably damaging	1.00
R5988:Fat1	UTSW	8	45029456	missense	probably benign	0.00
R6166:Fat1	UTSW	8	44952485	missense	probably damaging	1.00
R6184:Fat1	UTSW	8	44953392	missense	probably benign	0.00
R6208:Fat1	UTSW	8	45027613	missense	probably damaging	0.99
R6351:Fat1	UTSW	8	45033495	missense	probably damaging	1.00
R6391:Fat1	UTSW	8	44952342	missense	possibly damaging	0.69
R6701:Fat1	UTSW	8	44950681	missense	probably damaging	1.00
R6702:Fat1	UTSW	8	44953046	missense	probably benign	0.28
R6703:Fat1	UTSW	8	44953046	missense	probably benign	0.28
R6704:Fat1	UTSW	8	45024373	missense	probably damaging	1.00
R6822:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R6852:Fat1	UTSW	8	45035598	missense	possibly damaging	0.46
R6863:Fat1	UTSW	8	45044464	missense	probably damaging	1.00
R6885:Fat1	UTSW	8	44952452	missense	possibly damaging	0.94
R6912:Fat1	UTSW	8	45051023	missense	probably benign	0.00
R6927:Fat1	UTSW	8	45024495	missense	probably benign	0.41
R6964:Fat1	UTSW	8	45043945	missense	probably damaging	1.00
R7010:Fat1	UTSW	8	44953349	nonsense	probably null
R7062:Fat1	UTSW	8	44950216	start codon destroyed	probably null	0.99
R7063:Fat1	UTSW	8	45040775	missense	probably benign	0.09
R7071:Fat1	UTSW	8	44989108	missense	possibly damaging	0.67
R7117:Fat1	UTSW	8	45031468	missense	probably damaging	0.98
R7146:Fat1	UTSW	8	44950925	missense	probably benign
R7210:Fat1	UTSW	8	45023503	missense	probably damaging	1.00
R7227:Fat1	UTSW	8	45010609	missense	probably benign	0.08
R7270:Fat1	UTSW	8	45037438	missense	probably damaging	1.00
R7373:Fat1	UTSW	8	45026665	missense	probably damaging	1.00
R7390:Fat1	UTSW	8	44952474	missense	possibly damaging	0.81
R7465:Fat1	UTSW	8	45044152	missense	probably benign	0.35
R7476:Fat1	UTSW	8	45031274	missense	probably benign	0.01
R7483:Fat1	UTSW	8	45023160	missense	probably benign	0.13
R7484:Fat1	UTSW	8	45036184	missense	probably damaging	1.00
R7526:Fat1	UTSW	8	45023427	missense	probably damaging	1.00
R7549:Fat1	UTSW	8	44988994	missense	probably benign	0.01
R7554:Fat1	UTSW	8	45037165	missense	possibly damaging	0.88
R7620:Fat1	UTSW	8	45009850	missense	possibly damaging	0.95
R7652:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R7694:Fat1	UTSW	8	44988930	critical splice acceptor site	probably null
R7746:Fat1	UTSW	8	44951633	missense	probably damaging	0.96
R7762:Fat1	UTSW	8	45023322	missense	probably damaging	1.00
R7782:Fat1	UTSW	8	44950911	missense	probably damaging	1.00
R7801:Fat1	UTSW	8	45042223	missense	probably damaging	1.00
R7807:Fat1	UTSW	8	45041973	missense	probably damaging	1.00
R7821:Fat1	UTSW	8	44950224	missense	probably benign
R7869:Fat1	UTSW	8	45051222	missense	probably benign	0.02
R8034:Fat1	UTSW	8	44951691	missense	probably benign	0.28
R8094:Fat1	UTSW	8	44952702	missense	probably damaging	0.98
R8111:Fat1	UTSW	8	45026058	missense	possibly damaging	0.94
R8220:Fat1	UTSW	8	45039956	missense	probably null
R8221:Fat1	UTSW	8	44953353	missense
R8233:Fat1	UTSW	8	44952018	missense
R8250:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R8279:Fat1	UTSW	8	45030347	critical splice donor site	probably null
R8726:Fat1	UTSW	8	45024169	missense	probably benign	0.23
R8875:Fat1	UTSW	8	45040563	missense	probably damaging	1.00
R8937:Fat1	UTSW	8	45030313	missense	probably damaging	1.00
R8950:Fat1	UTSW	8	45023121	missense	probably damaging	1.00
R8971:Fat1	UTSW	8	45042294	missense	probably damaging	1.00
R8976:Fat1	UTSW	8	45031295	missense	probably benign	0.02
R9000:Fat1	UTSW	8	45044550	nonsense	probably null
R9032:Fat1	UTSW	8	45039857	missense	probably benign	0.01
R9076:Fat1	UTSW	8	45039901	missense	probably damaging	1.00
R9083:Fat1	UTSW	8	45013090	missense	possibly damaging	0.76
R9083:Fat1	UTSW	8	45038299	missense	probably benign	0.00
R9103:Fat1	UTSW	8	44951813	missense	probably benign	0.38
R9124:Fat1	UTSW	8	44950326	missense	probably benign
R9124:Fat1	UTSW	8	45025027	missense	possibly damaging	0.48
R9128:Fat1	UTSW	8	45009841	missense	probably benign	0.14
R9148:Fat1	UTSW	8	44952645	missense	possibly damaging	0.81
R9162:Fat1	UTSW	8	44951315	missense	probably damaging	1.00
R9209:Fat1	UTSW	8	44951754	missense	possibly damaging	0.80
R9276:Fat1	UTSW	8	45035477	missense	probably damaging	0.99
R9303:Fat1	UTSW	8	45010461	missense	probably damaging	1.00
R9319:Fat1	UTSW	8	44953023	missense	probably damaging	1.00
R9392:Fat1	UTSW	8	45023191	missense	probably damaging	1.00
R9616:Fat1	UTSW	8	44953038	missense	probably damaging	0.99
R9712:Fat1	UTSW	8	45017380	missense	probably benign	0.05
R9756:Fat1	UTSW	8	45043937	missense	probably damaging	0.96
RF001:Fat1	UTSW	8	44988966	missense	probably benign	0.00
X0064:Fat1	UTSW	8	45025734	missense	possibly damaging	0.58
Z1088:Fat1	UTSW	8	45023807	missense	possibly damaging	0.88
Z1176:Fat1	UTSW	8	44950598	missense	probably benign
Z1176:Fat1	UTSW	8	45023596	missense	possibly damaging	0.65
Z1176:Fat1	UTSW	8	45036838	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTACTGGCGCAACTTCCAG -3'
(R):5'- GCTCTTCTCCACATACATAGGCG -3'

Sequencing Primer
(F):5'- TGACATACAGATCGTCAGCCTG -3'
(R):5'- CATAGGCGCTATTACCTTTGCAGAG -3'

Posted On

2019-11-26