Mutagenetix > Incidental Mutation

Incidental Mutation 'R1783:Pik3c2b'

195524

Institutional Source

Beutler Lab

Gene Symbol

Pik3c2b

Ensembl Gene

ENSMUSG00000026447

Gene Name

phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta

Synonyms

C330011J12Rik, PI3K-C2beta

MMRRC Submission

039814-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.251) question?

Stock #

R1783 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

133045667-133108687 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 133066627 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 110 (P110S)

Ref Sequence

ENSEMBL: ENSMUSP00000115469 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077730] [ENSMUST00000153707]

AlphaFold

E9QAN8

Predicted Effect

probably benign
Transcript: ENSMUST00000077730
AA Change: P110S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000076911
Gene: ENSMUSG00000026447
AA Change: P110S

Domain	Start	End	E-Value	Type
low complexity region	155	160	N/A	INTRINSIC
low complexity region	168	183	N/A	INTRINSIC
PI3K_rbd	363	465	2.15e-19	SMART
PI3K_C2	618	726	6.17e-29	SMART
PI3Ka	804	990	1.66e-84	SMART
PI3Kc	1078	1340	3.45e-132	SMART
PX	1364	1476	9.44e-27	SMART
low complexity region	1481	1492	N/A	INTRINSIC
C2	1517	1622	1.82e-18	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000124934

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145153

Predicted Effect

probably benign
Transcript: ENSMUST00000153707
AA Change: P110S

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000115469
Gene: ENSMUSG00000026447
AA Change: P110S

Domain	Start	End	E-Value	Type
low complexity region	155	160	N/A	INTRINSIC
low complexity region	168	183	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186515

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.5%
20x: 93.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit normal epidermal growth, differentiation and function. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 226 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921539E11Rik	T	A	4: 103,231,089	T307S	probably damaging	Het
4930590J08Rik	T	C	6: 91,919,278	I369T	possibly damaging	Het
Abhd17a	T	A	10: 80,584,026	I115F	probably benign	Het
Acacb	TGGGG	TGGG	5: 114,209,767		probably null	Het
Adgrb2	C	T	4: 130,009,305	T566I	possibly damaging	Het
Adgrf4	C	T	17: 42,666,898	R518Q	possibly damaging	Het
Aspm	A	G	1: 139,473,574	I1111V	probably benign	Het
Bbs9	T	C	9: 22,659,119	V587A	possibly damaging	Het
C4bp	C	G	1: 130,642,988	V284L	probably benign	Het
Cacna1s	T	C	1: 136,118,716	F1761S	probably benign	Het
Camsap2	C	T	1: 136,281,315	R802Q	probably benign	Het
Capn8	T	C	1: 182,598,822	S241P	probably damaging	Het
Capn9	G	A	8: 124,605,711	G430R	possibly damaging	Het
Cars	C	A	7: 143,592,474	R71M	probably damaging	Het
Ccdc93	C	T	1: 121,456,126	P192L	probably benign	Het
Ccdc93	T	C	1: 121,461,939	V237A	probably benign	Het
Cd55	C	T	1: 130,449,423	V333I	probably benign	Het
Cd55	C	A	1: 130,459,633	A143S	probably benign	Het
Cdh19	C	A	1: 110,893,384	E541D	probably damaging	Het
Cdh7	C	G	1: 110,065,735	L307V	possibly damaging	Het
Cep350	A	C	1: 155,928,865	L824R	probably damaging	Het
Cfh	C	T	1: 140,147,697	V268I	possibly damaging	Het
Cfhr2	A	G	1: 139,813,442	M265T	probably benign	Het
Cfhr2	A	C	1: 139,813,459	N259K	probably benign	Het
Chil1	C	T	1: 134,188,529	A250V	probably damaging	Het
Chit1	G	T	1: 134,149,395	R312I	probably benign	Het
Chit1	A	G	1: 134,149,394	R312G	possibly damaging	Het
Cntnap5a	C	A	1: 116,455,004	L1001I	probably benign	Het
Cntnap5a	T	C	1: 116,455,101	L1033S	probably benign	Het
Cntnap5a	C	T	1: 116,455,143	T1047I	probably benign	Het
Crb1	T	C	1: 139,234,779	M1214V	probably benign	Het
Crb1	A	T	1: 139,237,622	H921Q	probably benign	Het
Crb1	G	A	1: 139,241,138	P881S	probably damaging	Het
Crb1	C	T	1: 139,242,995	G825R	probably damaging	Het
Crb1	C	T	1: 139,243,417	R684H	probably benign	Het
Cxcr4	C	T	1: 128,589,277	V216I	probably benign	Het
Cyb5r1	C	T	1: 134,407,667	R147W	probably damaging	Het
Ddx59	T	C	1: 136,417,053	V154A	probably benign	Het
Dock10	T	G	1: 80,574,180	Y659S	probably benign	Het
Dsel	T	C	1: 111,859,457	N1116S	probably benign	Het
Dsel	G	C	1: 111,859,994	T937S	probably benign	Het
Dsg2	G	A	18: 20,591,880	V448I	probably benign	Het
Dstyk	C	T	1: 132,456,984	L739F	probably damaging	Het
Efnb2	G	T	8: 8,623,237	T140K	probably damaging	Het
En1	A	G	1: 120,603,621	S197G	unknown	Het
Eogt	T	C	6: 97,113,864	D438G	probably damaging	Het
Etnk2	A	G	1: 133,363,923	S54G	probably benign	Het
Etnk2	C	A	1: 133,365,587	D89E	probably benign	Het
Etnk2	G	T	1: 133,365,765	G149W	probably damaging	Het
Etnk2	C	T	1: 133,365,816	R166*	probably null	Het
Etnk2	G	A	1: 133,365,817	R166Q	probably benign	Het
Etnk2	T	A	1: 133,376,915	V292E	probably benign	Het
Etnk2	G	T	1: 133,377,046	A336S	probably benign	Het
Etnppl	A	G	3: 130,620,749	T98A	probably damaging	Het
Fam131b	T	G	6: 42,318,580	Q221P	possibly damaging	Het
Fam72a	T	C	1: 131,530,668	I56T	probably benign	Het
Fam72a	C	T	1: 131,538,895	T139M	probably benign	Het
Fam90a1a	A	G	8: 21,963,463	N278S	probably benign	Het
Fbxo40	T	A	16: 36,966,222	M662L	probably damaging	Het
Fcamr	A	C	1: 130,804,627	N117T	probably benign	Het
Fcamr	A	G	1: 130,811,580	I206V	probably benign	Het
Fcamr	G	A	1: 130,812,629	G262S	probably benign	Het
Fcamr	A	G	1: 130,812,692	I283V	probably benign	Het
Fcamr	T	C	1: 130,812,738	V298A	probably benign	Het
Fcamr	A	G	1: 130,812,809	M322V	probably benign	Het
Fcamr	C	T	1: 130,812,816	P324L	probably benign	Het
Fcamr	A	G	1: 130,814,597	N574D	probably benign	Het
Fcmr	A	G	1: 130,875,974	T172A	probably benign	Het
Fcmr	T	C	1: 130,878,269	S321P	probably benign	Het
Fmo9	A	T	1: 166,673,648	F192L	probably benign	Het
Gabarap	C	T	11: 69,991,689		probably benign	Het
Gatad2a	G	T	8: 69,909,936	H600N	probably damaging	Het
Gemin4	G	C	11: 76,211,050	P962A	probably damaging	Het
Git2	T	C	5: 114,739,124	E99G	probably damaging	Het
Gli2	C	T	1: 118,868,087	A113T	possibly damaging	Het
Gli2	G	T	1: 119,002,044	H44Q	probably benign	Het
Glrx2	C	T	1: 143,739,740	A27V	possibly damaging	Het
Gm10961	T	C	3: 107,632,994		probably benign	Het
Gm13119	G	T	4: 144,361,725	E30D	probably benign	Het
Gpr25	G	A	1: 136,260,710	P55L	probably benign	Het
Gpsm3	G	A	17: 34,590,754	R52H	possibly damaging	Het
Grm7	G	C	6: 111,358,295	D556H	probably damaging	Het
Hdac5	A	G	11: 102,200,516	F683L	probably benign	Het
Ift20	G	A	11: 78,540,034	E68K	probably damaging	Het
Igfn1	G	A	1: 135,959,928	P2466L	probably damaging	Het
Igfn1	G	A	1: 135,968,199	A1543V	probably benign	Het
Igfn1	T	C	1: 135,970,411	S806G	probably benign	Het
Igfn1	C	T	1: 135,972,127	R482Q	probably benign	Het
Igfn1	C	T	1: 135,979,915	A231T	probably benign	Het
Igfn1	G	A	1: 135,982,475	R124W	probably benign	Het
Igfn1	T	C	1: 135,998,625	E29G	probably benign	Het
Igfn1	T	C	1: 135,998,683	I10V	unknown	Het
Ikbke	C	A	1: 131,265,937	A459S	probably benign	Het
Ikbke	T	C	1: 131,269,823	S447G	probably benign	Het
Ipo9	ATCCTCCTCCTCCTCCTC	ATCCTCCTCCTCCTCCTCCTC	1: 135,386,268		probably benign	Het
Ipo9	A	G	1: 135,402,250	V484A	probably benign	Het
Irx5	A	C	8: 92,359,688	E133A	probably damaging	Het
Itgb5	C	T	16: 33,940,562	T589I	probably benign	Het
Jarid2	T	A	13: 44,906,276	N661K	probably damaging	Het
Kcna5	A	T	6: 126,533,860	I435N	probably damaging	Het
Kcnj5	T	C	9: 32,322,192	I276V	probably damaging	Het
Kcnt2	G	A	1: 140,354,547	S90N	probably benign	Het
Kif14	A	G	1: 136,468,279	N108D	probably benign	Het
Kif14	A	G	1: 136,468,975	K340E	probably damaging	Het
Kif14	G	A	1: 136,478,365	A556T	probably benign	Het
Kif14	A	G	1: 136,490,332	S868G	probably benign	Het
Kif14	C	T	1: 136,503,431	L1189F	probably benign	Het
Kif14	T	C	1: 136,515,961	F1291L	probably benign	Het
Kif14	T	C	1: 136,525,783	V1433A	probably benign	Het
Krt2	T	C	15: 101,813,973	R426G	probably damaging	Het
Lad1	C	T	1: 135,827,381	P132S	possibly damaging	Het
Lad1	C	T	1: 135,828,023	R346C	probably damaging	Het
Lax1	T	C	1: 133,679,978	R342G	probably benign	Het
Lax1	T	C	1: 133,680,569	N145D	probably benign	Het
Lax1	G	A	1: 133,683,634	P67S	probably damaging	Het
Lgr6	C	T	1: 134,987,088	V641I	probably benign	Het
Lgr6	G	T	1: 134,990,635	H263N	probably benign	Het
Lgr6	C	T	1: 135,003,476	S3N	probably benign	Het
Lgr6	A	T	1: 134,988,009	S334T	probably benign	Het
Lin7b	A	T	7: 45,369,927	H72Q	probably benign	Het
Lmod1	C	T	1: 135,364,073	T222I	probably benign	Het
Map3k9	A	T	12: 81,722,226	V1016E	probably damaging	Het
Mcam	T	C	9: 44,134,706	L6P	probably damaging	Het
Mgam	A	G	6: 40,664,860	H549R	possibly damaging	Het
Miip	G	A	4: 147,865,774	P122S	probably damaging	Het
Mkrn1	T	A	6: 39,400,456	N282Y	probably null	Het
Mrc1	T	C	2: 14,327,844	V1285A	probably benign	Het
Mroh3	G	C	1: 136,192,144	Q440E	possibly damaging	Het
Mybpc1	T	C	10: 88,570,568	D152G	probably damaging	Het
Mybph	C	T	1: 134,197,480	R249C	probably benign	Het
Myh7b	A	G	2: 155,625,672	D739G	possibly damaging	Het
Nav1	A	T	1: 135,584,727	D198E	possibly damaging	Het
Nfkbib	A	T	7: 28,762,055	Y86N	probably damaging	Het
Nr5a2	C	A	1: 136,952,125	R35L	probably benign	Het
Nrip1	G	T	16: 76,292,890	T593K	probably benign	Het
Obscn	A	G	11: 59,073,633	Y726H	probably damaging	Het
Obsl1	G	A	1: 75,486,756	T1764M	probably benign	Het
Olfr1240	C	T	2: 89,439,583	R232H	probably benign	Het
Olfr1307	T	C	2: 111,945,288	H56R	probably benign	Het
Olfr924	T	A	9: 38,848,972	I286K	probably damaging	Het
Olfr951	T	C	9: 39,394,222	Y144H	probably benign	Het
Optc	A	T	1: 133,903,796		probably null	Het
Optc	C	G	1: 133,905,170	S64T	probably benign	Het
Pard6g	T	A	18: 80,079,825	F25I	probably damaging	Het
Parp6	C	A	9: 59,633,538	C291*	probably null	Het
Perm1	C	T	4: 156,218,531	R511*	probably null	Het
Pigr	C	T	1: 130,844,522	A159V	possibly damaging	Het
Plekha6	C	G	1: 133,287,846	T792S	probably benign	Het
Pole	T	A	5: 110,297,430	L481Q	probably damaging	Het
Polr2i	T	C	7: 30,233,068	C67R	probably damaging	Het
Ppfia4	G	A	1: 134,299,321	P1159S	probably benign	Het
Prelp	C	T	1: 133,915,131	R92K	probably benign	Het
Ptgfrn	T	C	3: 101,056,442	N618S	possibly damaging	Het
Ptpn7	A	G	1: 135,134,475	Q53R	probably benign	Het
Ptprc	T	G	1: 138,099,676	N478T	probably benign	Het
Ptprc	A	G	1: 138,107,823	S405P	probably benign	Het
Ptprc	C	A	1: 138,107,824	E402D	probably benign	Het
Ptprc	A	G	1: 138,107,837	V400A	probably benign	Het
Ptprc	T	C	1: 138,112,254	K212E	possibly damaging	Het
Rab29	A	G	1: 131,872,110	Q141R	probably benign	Het
Ren1	T	A	1: 133,354,206	W22R	probably damaging	Het
Ren1	C	T	1: 133,354,237	T32I	probably benign	Het
Ren1	A	T	1: 133,359,079	E315D	probably benign	Het
Ren1	A	T	1: 133,359,983	N352Y	probably benign	Het
Ren1	C	G	1: 133,360,007	L360V	probably benign	Het
Ren1	C	G	1: 133,350,778		probably null	Het
Rims1	C	T	1: 22,346,529		probably null	Het
Rnpep	C	T	1: 135,263,096	A571T	possibly damaging	Het
Rnpep	G	C	1: 135,283,977	A11G	probably benign	Het
Rps19bp1	CCTTCTTCTTCTTCTTCTTCTT	CCTTCTTCTTCTTCTTCTT	15: 80,261,049		probably benign	Het
Rsph4a	T	C	10: 33,911,636	I596T	probably damaging	Het
Ryr2	A	T	13: 11,700,371	Y2770*	probably null	Het
Sctr	T	C	1: 120,031,656	F110L	probably benign	Het
Sept4	A	T	11: 87,583,436	Q60L	probably benign	Het
Serpinb10	C	T	1: 107,538,473	S63F	probably damaging	Het
Serpinb2	G	A	1: 107,515,635	A55T	probably damaging	Het
Serpinb2	C	A	1: 107,523,834	A239E	probably benign	Het
Serpinb2	C	T	1: 107,523,890	H258Y	probably benign	Het
Serpinb2	C	T	1: 107,523,894	T259I	probably benign	Het
Serpinb2	A	C	1: 107,524,543	S284R	probably benign	Het
Serpinb8	A	G	1: 107,597,527	S20G	probably benign	Het
Serpinb8	G	A	1: 107,598,954	A75T	probably benign	Het
Serpinb8	A	C	1: 107,607,004	L268F	probably benign	Het
Sertad4	T	C	1: 192,847,032	T159A	probably benign	Het
Setd1a	T	A	7: 127,785,124	Y382*	probably null	Het
Sfswap	T	A	5: 129,513,240	V267E	possibly damaging	Het
Shank1	T	A	7: 44,352,737	D1293E	possibly damaging	Het
Slc26a9	C	T	1: 131,763,870	A617V	probably benign	Het
Slc26a9	C	A	1: 131,766,012	R747S	probably benign	Het
Slc36a1	A	G	11: 55,223,672	D192G	probably damaging	Het
Slc4a4	T	A	5: 89,132,414	L366Q	probably damaging	Het
Sorcs1	T	C	19: 50,228,309		probably null	Het
Steap3	T	C	1: 120,227,750	N493S	probably benign	Het
Steap3	G	A	1: 120,234,378	A350V	probably benign	Het
Svep1	T	A	4: 58,073,333	Y1992F	probably benign	Het
Syce1l	A	G	8: 113,654,834	R152G	possibly damaging	Het
Tbc1d17	A	C	7: 44,845,131	S227A	probably damaging	Het
Telo2	A	T	17: 25,102,738		probably null	Het
Thsd7b	C	T	1: 129,628,891	T328I	probably damaging	Het
Thsd7b	T	A	1: 129,667,937	F498Y	probably benign	Het
Thsd7b	G	C	1: 129,678,183	A554P	probably benign	Het
Thsd7b	A	C	1: 130,116,631	Q1116P	probably benign	Het
Tmem143	G	A	7: 45,907,002	D144N	possibly damaging	Het
Tmprss11a	T	A	5: 86,420,032	I279F	probably damaging	Het
Tnc	A	G	4: 64,018,096	L201P	probably damaging	Het
Tnnt2	C	T	1: 135,845,506		probably benign	Het
Trove2	C	T	1: 143,760,014	V465I	probably benign	Het
Trove2	T	C	1: 143,760,034	D458G	probably benign	Het
Ttn	C	T	2: 76,813,339	G11436R	probably damaging	Het
Ttn	A	G	2: 76,788,901		probably null	Het
Ube2t	C	T	1: 134,972,167	A149V	probably benign	Het
Unc80	G	A	1: 66,683,273	M3015I	probably benign	Het
Usp9y	A	T	Y: 1,367,093	V998D	probably benign	Het
Utrn	A	G	10: 12,463,339	F2858S	probably damaging	Het
Vmn1r224	T	C	17: 20,419,185	I8T	probably benign	Het
Vmn2r107	T	C	17: 20,356,513	S258P	possibly damaging	Het
Wars	A	C	12: 108,875,741	F160C	probably damaging	Het
Xirp1	T	G	9: 120,016,907	Q970P	probably benign	Het
Zc3h11a	G	A	1: 133,622,154	P695S	probably benign	Het
Zc3h11a	C	T	1: 133,624,621	V583I	probably benign	Het
Zfp28	A	T	7: 6,394,792	Y742F	probably damaging	Het
Zfp541	A	G	7: 16,077,973	T184A	probably damaging	Het
Zfp623	T	C	15: 75,947,911	S239P	probably damaging	Het
Zfp804b	T	C	5: 6,771,938	D375G	probably damaging	Het
Zp3r	A	G	1: 130,596,814	L164P	probably benign	Het
Zp3r	C	A	1: 130,619,414	E8D	possibly damaging	Het

Other mutations in Pik3c2b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01086:Pik3c2b	APN	1	133091618	missense	probably damaging	0.98
IGL01288:Pik3c2b	APN	1	133094805	missense	probably damaging	0.96
IGL01313:Pik3c2b	APN	1	133071631	nonsense	probably null
IGL01367:Pik3c2b	APN	1	133105988	missense	probably benign	0.02
IGL02379:Pik3c2b	APN	1	133094791	missense	probably damaging	1.00
IGL02638:Pik3c2b	APN	1	133077318	splice site	probably benign
IGL02728:Pik3c2b	APN	1	133092327	missense	probably benign	0.09
IGL02992:Pik3c2b	APN	1	133066980	nonsense	probably null
IGL03121:Pik3c2b	APN	1	133079745	missense	probably benign	0.00
R0453:Pik3c2b	UTSW	1	133077396	missense	probably damaging	1.00
R0518:Pik3c2b	UTSW	1	133105992	missense	probably damaging	1.00
R0616:Pik3c2b	UTSW	1	133100831	missense	probably damaging	1.00
R0659:Pik3c2b	UTSW	1	133071200	missense	probably damaging	0.99
R1542:Pik3c2b	UTSW	1	133090034	missense	probably damaging	1.00
R1716:Pik3c2b	UTSW	1	133094826	missense	probably damaging	1.00
R1728:Pik3c2b	UTSW	1	133066627	missense	probably benign	0.00
R1729:Pik3c2b	UTSW	1	133066627	missense	probably benign	0.00
R1730:Pik3c2b	UTSW	1	133066627	missense	probably benign	0.00
R1739:Pik3c2b	UTSW	1	133066627	missense	probably benign	0.00
R1762:Pik3c2b	UTSW	1	133066627	missense	probably benign	0.00
R1784:Pik3c2b	UTSW	1	133066627	missense	probably benign	0.00
R1785:Pik3c2b	UTSW	1	133066627	missense	probably benign	0.00
R1816:Pik3c2b	UTSW	1	133101370	missense	probably benign	0.00
R1897:Pik3c2b	UTSW	1	133066916	missense	possibly damaging	0.57
R2006:Pik3c2b	UTSW	1	133066544	missense	probably damaging	1.00
R2067:Pik3c2b	UTSW	1	133099611	missense	probably damaging	1.00
R2271:Pik3c2b	UTSW	1	133103428	missense	probably benign
R2294:Pik3c2b	UTSW	1	133066775	missense	probably damaging	1.00
R2320:Pik3c2b	UTSW	1	133103413	missense	probably damaging	1.00
R4735:Pik3c2b	UTSW	1	133067049	missense	probably benign	0.25
R4926:Pik3c2b	UTSW	1	133099626	nonsense	probably null
R4948:Pik3c2b	UTSW	1	133099715	critical splice donor site	probably null
R4997:Pik3c2b	UTSW	1	133105081	missense	probably damaging	1.00
R5304:Pik3c2b	UTSW	1	133070408	missense	possibly damaging	0.50
R5461:Pik3c2b	UTSW	1	133099702	missense	possibly damaging	0.66
R5722:Pik3c2b	UTSW	1	133103836	missense	probably damaging	1.00
R5971:Pik3c2b	UTSW	1	133074627	splice site	probably null
R5980:Pik3c2b	UTSW	1	133088308	missense	probably benign	0.43
R6036:Pik3c2b	UTSW	1	133090713	missense	possibly damaging	0.95
R6138:Pik3c2b	UTSW	1	133074627	splice site	probably null
R6223:Pik3c2b	UTSW	1	133070357	missense	probably damaging	1.00
R6273:Pik3c2b	UTSW	1	133066711	missense	probably benign	0.02
R6742:Pik3c2b	UTSW	1	133075821	missense	probably benign
R6954:Pik3c2b	UTSW	1	133066303	missense	possibly damaging	0.50
R6998:Pik3c2b	UTSW	1	133102372	missense	probably benign	0.23
R7103:Pik3c2b	UTSW	1	133105974	missense	probably damaging	1.00
R7133:Pik3c2b	UTSW	1	133090234	missense	possibly damaging	0.73
R7161:Pik3c2b	UTSW	1	133106112	missense	probably damaging	0.98
R7183:Pik3c2b	UTSW	1	133066465	missense	probably benign	0.00
R7193:Pik3c2b	UTSW	1	133079774	missense	probably benign	0.00
R7252:Pik3c2b	UTSW	1	133094734	missense	probably benign	0.19
R7263:Pik3c2b	UTSW	1	133090202	missense	probably damaging	0.98
R7404:Pik3c2b	UTSW	1	133090706	missense	probably damaging	1.00
R7709:Pik3c2b	UTSW	1	133079841	critical splice donor site	probably null
R7712:Pik3c2b	UTSW	1	133085611	missense	probably damaging	1.00
R7823:Pik3c2b	UTSW	1	133102305	missense	probably damaging	1.00
R7831:Pik3c2b	UTSW	1	133071242	missense	possibly damaging	0.94
R7913:Pik3c2b	UTSW	1	133090061	critical splice donor site	probably null
R7916:Pik3c2b	UTSW	1	133100904	missense	probably benign	0.30
R7960:Pik3c2b	UTSW	1	133103849	missense	probably damaging	1.00
R7981:Pik3c2b	UTSW	1	133075809	critical splice acceptor site	probably null
R8346:Pik3c2b	UTSW	1	133090246	missense	probably damaging	0.97
R8938:Pik3c2b	UTSW	1	133088330	missense	probably benign	0.19
R8997:Pik3c2b	UTSW	1	133090779	missense	possibly damaging	0.83
R9416:Pik3c2b	UTSW	1	133077449	missense	probably damaging	1.00
R9598:Pik3c2b	UTSW	1	133084987	critical splice donor site	probably null
R9621:Pik3c2b	UTSW	1	133071607	missense	probably damaging	1.00
R9742:Pik3c2b	UTSW	1	133094749	missense	probably damaging	1.00
R9776:Pik3c2b	UTSW	1	133090850	missense	possibly damaging	0.64
R9786:Pik3c2b	UTSW	1	133091600	missense	possibly damaging	0.94
U15987:Pik3c2b	UTSW	1	133074627	splice site	probably null
X0060:Pik3c2b	UTSW	1	133084936	missense	probably benign	0.18
Z1176:Pik3c2b	UTSW	1	133066553	missense	probably damaging	1.00
Z1176:Pik3c2b	UTSW	1	133099686	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GATGAGCCAGCCTTGGACTTCTAC -3'
(R):5'- GGCTGTTCTGCCGAAGAGAAACTG -3'

Sequencing Primer
(F):5'- TTGGACTTCTACAGCAAGCC -3'
(R):5'- GCAAGGGAGGGGCATCC -3'

Posted On

2014-05-23