Mutagenetix > Incidental Mutation

Incidental Mutation 'R6987:Zfp882'

543031

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

045094-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.167) question?

Stock #

R6987 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

72662452-72670198 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 72668517 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 448 (V448E)

Ref Sequence

ENSEMBL: ENSMUSP00000105629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

probably benign
Transcript: ENSMUST00000110002
AA Change: V448E

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: V448E

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125802

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000126607

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.8%
10x: 99.0%
20x: 96.9%

Validation Efficiency

100% (47/47)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam10	A	G	9: 70,629,978 (GRCm39)	I137V	probably benign	Het
Agtr1b	A	C	3: 20,370,585 (GRCm39)	I7S	probably benign	Het
Akt2	G	T	7: 27,332,666 (GRCm39)	V215L	probably damaging	Het
Ccdc148	C	A	2: 58,872,926 (GRCm39)	L294F	probably damaging	Het
Ccdc3	T	A	2: 5,143,115 (GRCm39)	V124E	possibly damaging	Het
Cldnd1	A	G	16: 58,551,734 (GRCm39)	D121G	probably benign	Het
Cnr1	A	G	4: 33,944,739 (GRCm39)	I376V	probably benign	Het
Cyp2c40	A	G	19: 39,801,211 (GRCm39)		probably benign	Het
Dnah8	A	G	17: 30,881,065 (GRCm39)	I601V	possibly damaging	Het
Dnhd1	C	A	7: 105,353,792 (GRCm39)	H2982N	probably damaging	Het
Elavl4	A	T	4: 110,108,602 (GRCm39)	D55E	possibly damaging	Het
Enc1	T	C	13: 97,382,144 (GRCm39)	I218T	probably benign	Het
Fbln2	T	A	6: 91,211,211 (GRCm39)	V385D	probably benign	Het
Ffar2	A	G	7: 30,519,108 (GRCm39)	V144A	possibly damaging	Het
Fsip2	C	T	2: 82,778,630 (GRCm39)	Q159*	probably null	Het
Golga4	A	G	9: 118,387,600 (GRCm39)	H1574R	probably benign	Het
Herc2	G	A	7: 55,756,201 (GRCm39)	R747H	possibly damaging	Het
Lama4	A	G	10: 38,950,275 (GRCm39)	N985D	probably benign	Het
Lrp1	T	C	10: 127,410,874 (GRCm39)	N1438S	probably damaging	Het
Masp1	C	A	16: 23,332,665 (GRCm39)	V57F	probably damaging	Het
Mypn	T	C	10: 63,028,910 (GRCm39)	E51G	probably benign	Het
Nos1	C	T	5: 118,033,850 (GRCm39)	T324M	probably benign	Het
Npas3	A	G	12: 54,115,036 (GRCm39)	K635E	possibly damaging	Het
Or5p4	C	A	7: 107,680,338 (GRCm39)	C112*	probably null	Het
Or8b12i	A	T	9: 20,082,130 (GRCm39)	S246T	probably benign	Het
Osbp2	T	C	11: 3,667,958 (GRCm39)	E13G	probably damaging	Het
Pira12	A	G	7: 3,900,660 (GRCm39)	I30T	probably damaging	Het
Pkd1l1	A	G	11: 8,852,575 (GRCm39)	M636T	probably benign	Het
Pla2g4e	C	T	2: 120,016,861 (GRCm39)	A227T	probably benign	Het
Prex2	C	A	1: 11,240,976 (GRCm39)	A1028E	probably damaging	Het
Prr14	A	G	7: 127,072,977 (GRCm39)	D49G	possibly damaging	Het
Slc9a9	A	G	9: 94,552,043 (GRCm39)		probably benign	Het
Sptb	A	G	12: 76,660,021 (GRCm39)	C960R	probably benign	Het
Taf15	C	A	11: 83,375,521 (GRCm39)	T31K	possibly damaging	Het
Tdrd9	C	A	12: 111,992,027 (GRCm39)	Q601K	possibly damaging	Het
Tes	C	A	6: 17,086,154 (GRCm39)	Q16K	probably benign	Het
Ticam1	C	T	17: 56,576,900 (GRCm39)	E732K	probably benign	Het
Tmem168	T	A	6: 13,591,476 (GRCm39)	M63L	possibly damaging	Het
Trav14-1	C	T	14: 53,791,916 (GRCm39)	R89*	probably null	Het
Trp53bp2	A	T	1: 182,274,200 (GRCm39)	Y615F	probably damaging	Het
Ttc27	T	C	17: 75,084,736 (GRCm39)		probably null	Het
Usp25	T	A	16: 76,874,068 (GRCm39)	V548E	probably damaging	Het
Vmn1r124	A	T	7: 20,993,743 (GRCm39)	I267K	probably benign	Het
Vmn1r69	A	G	7: 10,314,491 (GRCm39)	M1T	probably null	Het
Zfp729a	T	A	13: 67,768,058 (GRCm39)	K724*	probably null	Het
Zfp850	A	T	7: 27,689,426 (GRCm39)	C261S	probably damaging	Het
Zzef1	A	G	11: 72,746,340 (GRCm39)	M881V	possibly damaging	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	72,667,671 (GRCm39)	missense	probably benign
R0244:Zfp882	UTSW	8	72,667,367 (GRCm39)	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	72,668,459 (GRCm39)	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	72,668,181 (GRCm39)	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	72,668,530 (GRCm39)	nonsense	probably null
R1299:Zfp882	UTSW	8	72,667,317 (GRCm39)	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	72,667,453 (GRCm39)	missense	probably damaging	0.97
R4829:Zfp882	UTSW	8	72,668,233 (GRCm39)	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	72,668,498 (GRCm39)	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	72,668,204 (GRCm39)	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	72,667,303 (GRCm39)	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	72,666,999 (GRCm39)	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	72,668,349 (GRCm39)	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	72,668,484 (GRCm39)	missense	probably damaging	1.00
R6888:Zfp882	UTSW	8	72,668,130 (GRCm39)	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	72,667,093 (GRCm39)	critical splice donor site	probably null
R7780:Zfp882	UTSW	8	72,668,073 (GRCm39)	missense	possibly damaging	0.89
R7793:Zfp882	UTSW	8	72,666,985 (GRCm39)	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	72,667,962 (GRCm39)	missense	probably benign	0.00
R9452:Zfp882	UTSW	8	72,668,831 (GRCm39)	missense	probably damaging	1.00
R9694:Zfp882	UTSW	8	72,667,915 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GCCTATGGCTGTAAGCAGTG -3'
(R):5'- TGGCTTACACACAGTGCAC -3'

Sequencing Primer
(F):5'- TAAGCAGTGTGGGAAAGCCTTTG -3'
(R):5'- GGAACCATGAGTACTGTAGGATTTCC -3'

Posted On

2018-11-28