Incidental Mutation 'R6642:Gpr149'
| ID |
525835 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr149
|
| Ensembl Gene |
ENSMUSG00000043441 |
| Gene Name |
G protein-coupled receptor 149 |
| Synonyms |
PGR10, 9630018L10Rik, R35, Ieda |
| MMRRC Submission |
044763-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.074)
|
| Stock # |
R6642 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
3 |
| Chromosomal Location |
62436851-62512861 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to T
at 62437995 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Alanine to Threonine
at position 721
(A721T)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000060893
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000058535]
|
| AlphaFold |
Q3UVY1 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000058535
AA Change: A721T
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000060893
Gene: ENSMUSG00000043441
AA Change: A721T
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_1
|
52 |
363 |
7.2e-7 |
PFAM |
|
coiled coil region
|
694 |
730 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.1671 |
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.5%
- 10x: 97.8%
- 20x: 93.3%
|
| Validation Efficiency |
100% (35/35) |
| MGI Phenotype |
PHENOTYPE: Female mice homozygous for a knock-out allele exhibit increased fertility with increased litter size and frequency. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 34 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 6820408C15Rik |
T |
A |
2: 152,282,790 (GRCm39) |
V215D |
probably damaging |
Het |
| Ablim1 |
A |
G |
19: 57,119,284 (GRCm39) |
S267P |
probably benign |
Het |
| Adcy2 |
C |
T |
13: 68,768,945 (GRCm39) |
C1061Y |
probably damaging |
Het |
| Aip |
C |
A |
19: 4,165,149 (GRCm39) |
C240F |
probably damaging |
Het |
| Aldh1a2 |
A |
T |
9: 71,160,268 (GRCm39) |
D98V |
probably damaging |
Het |
| Arhgef40 |
T |
C |
14: 52,228,419 (GRCm39) |
|
probably benign |
Het |
| Cplx2 |
G |
T |
13: 54,526,736 (GRCm39) |
R48L |
probably damaging |
Het |
| Ctrl |
C |
T |
8: 106,659,451 (GRCm39) |
|
probably null |
Het |
| Dnhd1 |
A |
G |
7: 105,353,006 (GRCm39) |
T2720A |
probably benign |
Het |
| Fzd1 |
A |
G |
5: 4,805,696 (GRCm39) |
Y629H |
probably damaging |
Het |
| Gins1 |
T |
C |
2: 150,770,038 (GRCm39) |
|
probably null |
Het |
| Helb |
A |
T |
10: 119,920,835 (GRCm39) |
M1036K |
probably benign |
Het |
| Il16 |
A |
G |
7: 83,337,335 (GRCm39) |
F127L |
probably benign |
Het |
| Kctd20 |
A |
T |
17: 29,180,640 (GRCm39) |
H138L |
probably damaging |
Het |
| Kctd9 |
T |
A |
14: 67,962,122 (GRCm39) |
L55* |
probably null |
Het |
| Marf1 |
C |
T |
16: 13,950,611 (GRCm39) |
R925H |
probably benign |
Het |
| Mbip |
A |
T |
12: 56,389,191 (GRCm39) |
|
probably benign |
Het |
| Myo1c |
C |
T |
11: 75,562,461 (GRCm39) |
P918S |
probably benign |
Het |
| Nod1 |
T |
G |
6: 54,925,014 (GRCm39) |
D99A |
probably damaging |
Het |
| Olfm4 |
A |
G |
14: 80,259,107 (GRCm39) |
K419E |
probably damaging |
Het |
| Or6c214 |
A |
T |
10: 129,591,232 (GRCm39) |
L29Q |
probably damaging |
Het |
| Pik3r4 |
A |
G |
9: 105,521,845 (GRCm39) |
D137G |
probably benign |
Het |
| Prdm4 |
T |
C |
10: 85,743,682 (GRCm39) |
E191G |
probably benign |
Het |
| Rassf10 |
A |
T |
7: 112,554,784 (GRCm39) |
T462S |
probably benign |
Het |
| Rundc3b |
A |
T |
5: 8,629,071 (GRCm39) |
I110N |
probably damaging |
Het |
| Sgsm3 |
C |
T |
15: 80,893,901 (GRCm39) |
R479C |
probably damaging |
Het |
| Tmc7 |
A |
T |
7: 118,144,834 (GRCm39) |
Y575* |
probably null |
Het |
| Trim33 |
T |
C |
3: 103,244,830 (GRCm39) |
L310S |
probably damaging |
Het |
| Trpm2 |
C |
T |
10: 77,773,660 (GRCm39) |
R585Q |
probably benign |
Het |
| Ttn |
T |
C |
2: 76,565,740 (GRCm39) |
E28204G |
probably damaging |
Het |
| Vmn1r233 |
A |
T |
17: 21,214,002 (GRCm39) |
L316Q |
probably damaging |
Het |
| Vmn2r110 |
A |
T |
17: 20,803,779 (GRCm39) |
N265K |
possibly damaging |
Het |
| Xylb |
A |
G |
9: 119,196,559 (GRCm39) |
H114R |
probably damaging |
Het |
| Ywhaz |
T |
C |
15: 36,791,166 (GRCm39) |
Y19C |
probably damaging |
Het |
|
| Other mutations in Gpr149 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00330:Gpr149
|
APN |
3 |
62,438,094 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01339:Gpr149
|
APN |
3 |
62,511,718 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01399:Gpr149
|
APN |
3 |
62,511,852 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01954:Gpr149
|
APN |
3 |
62,438,348 (GRCm39) |
missense |
probably benign |
0.36 |
|
IGL02115:Gpr149
|
APN |
3 |
62,502,336 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL02218:Gpr149
|
APN |
3 |
62,437,952 (GRCm39) |
utr 3 prime |
probably benign |
|
|
IGL02592:Gpr149
|
APN |
3 |
62,511,231 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
IGL03393:Gpr149
|
APN |
3 |
62,511,366 (GRCm39) |
missense |
probably benign |
0.15 |
|
R0578:Gpr149
|
UTSW |
3 |
62,510,110 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
R1173:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1174:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1175:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1432:Gpr149
|
UTSW |
3 |
62,438,439 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1484:Gpr149
|
UTSW |
3 |
62,502,592 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1972:Gpr149
|
UTSW |
3 |
62,438,216 (GRCm39) |
missense |
probably benign |
0.39 |
|
R1973:Gpr149
|
UTSW |
3 |
62,438,216 (GRCm39) |
missense |
probably benign |
0.39 |
|
R2180:Gpr149
|
UTSW |
3 |
62,511,489 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2241:Gpr149
|
UTSW |
3 |
62,511,474 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3118:Gpr149
|
UTSW |
3 |
62,502,443 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3547:Gpr149
|
UTSW |
3 |
62,502,549 (GRCm39) |
missense |
probably benign |
0.01 |
|
R3548:Gpr149
|
UTSW |
3 |
62,502,549 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4206:Gpr149
|
UTSW |
3 |
62,511,924 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4332:Gpr149
|
UTSW |
3 |
62,511,794 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R4531:Gpr149
|
UTSW |
3 |
62,510,099 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4557:Gpr149
|
UTSW |
3 |
62,511,918 (GRCm39) |
missense |
probably benign |
0.02 |
|
R4557:Gpr149
|
UTSW |
3 |
62,438,291 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4593:Gpr149
|
UTSW |
3 |
62,510,151 (GRCm39) |
intron |
probably benign |
|
|
R5397:Gpr149
|
UTSW |
3 |
62,438,226 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6592:Gpr149
|
UTSW |
3 |
62,437,961 (GRCm39) |
missense |
probably benign |
0.02 |
|
R6845:Gpr149
|
UTSW |
3 |
62,511,942 (GRCm39) |
missense |
possibly damaging |
0.58 |
|
R7303:Gpr149
|
UTSW |
3 |
62,502,491 (GRCm39) |
missense |
possibly damaging |
0.59 |
|
R7659:Gpr149
|
UTSW |
3 |
62,511,256 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7682:Gpr149
|
UTSW |
3 |
62,438,160 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7803:Gpr149
|
UTSW |
3 |
62,438,136 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7904:Gpr149
|
UTSW |
3 |
62,502,356 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7943:Gpr149
|
UTSW |
3 |
62,438,132 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8844:Gpr149
|
UTSW |
3 |
62,502,572 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8919:Gpr149
|
UTSW |
3 |
62,438,478 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9043:Gpr149
|
UTSW |
3 |
62,511,360 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9209:Gpr149
|
UTSW |
3 |
62,511,093 (GRCm39) |
missense |
probably benign |
0.40 |
|
Z1177:Gpr149
|
UTSW |
3 |
62,511,380 (GRCm39) |
frame shift |
probably null |
|
|
Z1190:Gpr149
|
UTSW |
3 |
62,511,972 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
| Predicted Primers |
PCR Primer
(F):5'- ATGGCAGGTCATCCAAACAAATAG -3'
(R):5'- AAAGTCAGGTCTCCTTCTCTGC -3'
Sequencing Primer
(F):5'- AACAAATAGTAACTAGAAAACCAGGG -3'
(R):5'- GGAAAACAGATTCGTCTCCTGCG -3'
|
| Posted On |
2018-06-22 |
Incidental Mutation