Mutagenetix > Incidental Mutation

Incidental Mutation 'R7332:Mycbp2'

569322

Institutional Source

Beutler Lab

Gene Symbol

Mycbp2

Ensembl Gene

ENSMUSG00000033004

Gene Name

MYC binding protein 2

Synonyms

C130061D10Rik, Phr1, Pam

MMRRC Submission

045425-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7332 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

103113411-103346814 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 103197357 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 2217 (I2217F)

Ref Sequence

ENSEMBL: ENSMUSP00000124710 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159855] [ENSMUST00000160758]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000159855
AA Change: I2217F

PolyPhen 2 Score 0.969 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000124710
Gene: ENSMUSG00000033004
AA Change: I2217F

Domain	Start	End	E-Value	Type
low complexity region	5	27	N/A	INTRINSIC
low complexity region	47	55	N/A	INTRINSIC
low complexity region	100	127	N/A	INTRINSIC
low complexity region	178	191	N/A	INTRINSIC
Pfam:RCC1_2	683	712	1.4e-10	PFAM
low complexity region	737	750	N/A	INTRINSIC
low complexity region	793	815	N/A	INTRINSIC
Pfam:RCC1_2	942	971	5.5e-10	PFAM
Pfam:RCC1	958	1006	4.8e-15	PFAM
Pfam:PHR	1235	1385	8.2e-44	PFAM
Pfam:PHR	1723	1880	1.4e-43	PFAM
low complexity region	1935	1948	N/A	INTRINSIC
low complexity region	2182	2195	N/A	INTRINSIC
Pfam:Filamin	2261	2431	7.5e-9	PFAM
Pfam:SH3_3	2472	2539	4.1e-9	PFAM
internal_repeat_3	2612	2679	1.69e-7	PROSPERO
low complexity region	2701	2710	N/A	INTRINSIC
low complexity region	2884	2917	N/A	INTRINSIC
low complexity region	2970	2984	N/A	INTRINSIC
coiled coil region	3263	3290	N/A	INTRINSIC
low complexity region	3352	3365	N/A	INTRINSIC
low complexity region	3418	3433	N/A	INTRINSIC
low complexity region	3678	3695	N/A	INTRINSIC
APC10	3810	3968	1.11e-18	SMART
low complexity region	4103	4115	N/A	INTRINSIC
low complexity region	4190	4212	N/A	INTRINSIC
Blast:BBOX	4327	4370	7e-7	BLAST
RING	4496	4546	5.35e-5	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160758
AA Change: I2184F

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000124601
Gene: ENSMUSG00000033004
AA Change: I2184F

Domain	Start	End	E-Value	Type
low complexity region	14	22	N/A	INTRINSIC
low complexity region	67	94	N/A	INTRINSIC
low complexity region	145	158	N/A	INTRINSIC
Pfam:RCC1_2	650	679	1e-10	PFAM
low complexity region	704	717	N/A	INTRINSIC
low complexity region	760	782	N/A	INTRINSIC
Pfam:RCC1_2	909	938	1.5e-9	PFAM
Pfam:RCC1	925	973	1.3e-15	PFAM
Pfam:PHR	1202	1353	1.6e-50	PFAM
Pfam:PHR	1690	1848	3.1e-58	PFAM
low complexity region	1902	1915	N/A	INTRINSIC
low complexity region	2149	2162	N/A	INTRINSIC
Pfam:Filamin	2228	2398	7.6e-9	PFAM
Pfam:SH3_3	2439	2507	2.3e-10	PFAM
internal_repeat_3	2554	2621	2e-7	PROSPERO
low complexity region	2643	2652	N/A	INTRINSIC
low complexity region	2774	2807	N/A	INTRINSIC
low complexity region	2860	2874	N/A	INTRINSIC
coiled coil region	3153	3180	N/A	INTRINSIC
low complexity region	3242	3255	N/A	INTRINSIC
low complexity region	3308	3323	N/A	INTRINSIC
low complexity region	3568	3585	N/A	INTRINSIC
APC10	3700	3858	1.11e-18	SMART
low complexity region	3993	4005	N/A	INTRINSIC
low complexity region	4080	4102	N/A	INTRINSIC
Blast:BBOX	4217	4260	7e-7	BLAST
RING	4386	4436	5.35e-5	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (68/68)

MGI Phenotype

PHENOTYPE: Mice homozygous for a targeted allele exhibit neonatal lethality, defective diaphragm innervation, abnormal brain morphology and defective axonal guidance. [provided by MGI curators]

Allele List at MGI

All alleles(10) : Targeted, knock-out(1) Targeted, other(1) Gene trapped(5) Chemically induced(3)

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Afap1l2	A	G	19: 56,918,121 (GRCm38)	S449P	probably damaging	Het
Atp6v0c	A	C	17: 24,169,224 (GRCm38)	S21A	probably benign	Het
Cacna1e	T	C	1: 154,725,801 (GRCm38)	Y40C	possibly damaging	Het
Cdadc1	A	G	14: 59,575,764 (GRCm38)	I398T	possibly damaging	Het
Cfap44	T	A	16: 44,429,828 (GRCm38)	D756E	probably damaging	Het
Clcnkb	A	T	4: 141,413,932 (GRCm38)	L104Q	probably null	Het
Clk1	G	A	1: 58,412,694 (GRCm38)	H421Y	probably benign	Het
Cmpk2	A	T	12: 26,478,062 (GRCm38)	D426V	probably damaging	Het
Cmya5	A	G	13: 93,092,553 (GRCm38)	L2009S	possibly damaging	Het
Col5a2	C	G	1: 45,380,165 (GRCm38)	D1252H	probably damaging	Het
Coro1b	A	G	19: 4,149,357 (GRCm38)	K5R	probably benign	Het
Csmd2	A	G	4: 128,419,567 (GRCm38)	T1346A		Het
Csrp1	G	T	1: 135,739,411 (GRCm38)	W4L	probably benign	Het
Cyp3a25	A	G	5: 145,993,007 (GRCm38)	I184T	probably damaging	Het
Egfl7	C	A	2: 26,590,713 (GRCm38)	R128S	probably benign	Het
Fggy	A	T	4: 95,623,482 (GRCm38)	N157I	probably damaging	Het
Gldc	A	T	19: 30,116,526 (GRCm38)	L697Q	probably damaging	Het
Gm4952	T	A	19: 12,627,009 (GRCm38)	Y262N	probably damaging	Het
Gsap	T	A	5: 21,290,121 (GRCm38)	M821K	probably benign	Het
Hps1	T	C	19: 42,777,912 (GRCm38)		probably null	Het
Hsbp1l1	T	C	18: 80,236,823 (GRCm38)		probably benign	Het
Igfbp7	G	A	5: 77,351,956 (GRCm38)	T220I	probably damaging	Het
Ints3	T	C	3: 90,415,512 (GRCm38)	Q137R	probably damaging	Het
Kcna6	A	G	6: 126,739,329 (GRCm38)	F199S	possibly damaging	Het
Kirrel1	T	C	3: 87,088,398 (GRCm38)	I410V	probably benign	Het
Llgl2	T	C	11: 115,848,299 (GRCm38)	V332A	probably damaging	Het
Lrrc27	A	G	7: 139,242,745 (GRCm38)	I517M	probably damaging	Het
Mas1	T	C	17: 12,842,219 (GRCm38)	T106A	probably benign	Het
Mast4	T	A	13: 102,751,424 (GRCm38)	Y1159F	possibly damaging	Het
Mmp2	A	G	8: 92,850,152 (GRCm38)	D601G	probably damaging	Het
Naip6	A	G	13: 100,300,701 (GRCm38)	V438A	possibly damaging	Het
Or4g17	A	G	2: 111,379,393 (GRCm38)	H131R	not run	Het
Pdf	T	C	8: 107,048,541 (GRCm38)	R20G	probably benign	Het
Pfdn2	T	C	1: 171,356,594 (GRCm38)	L47P	probably damaging	Het
Pik3c2g	C	A	6: 139,896,255 (GRCm38)	N795K		Het
Ppip5k1	A	T	2: 121,311,969 (GRCm38)	V1333D	probably damaging	Het
Pramel51	T	C	12: 88,176,417 (GRCm38)	T339A	possibly damaging	Het
Prss44	A	T	9: 110,815,462 (GRCm38)	I213F	probably damaging	Het
Rbm47	A	G	5: 66,026,214 (GRCm38)	Y349H	probably damaging	Het
Rcl1	A	T	19: 29,130,696 (GRCm38)	T253S	probably benign	Het
Rdh1	A	G	10: 127,759,885 (GRCm38)		probably benign	Het
Scn7a	C	T	2: 66,692,554 (GRCm38)	W935*	probably null	Het
Sec24b	T	C	3: 130,041,393 (GRCm38)	N52S	probably benign	Het
Serpinb5	A	T	1: 106,872,361 (GRCm38)	I94L	probably benign	Het
Setx	T	C	2: 29,146,626 (GRCm38)	V1041A	probably benign	Het
Slco3a1	G	A	7: 74,318,484 (GRCm38)	A496V	possibly damaging	Het
Spag5	T	A	11: 78,313,379 (GRCm38)	L486*	probably null	Het
Spink5	T	C	18: 43,982,250 (GRCm38)	I183T	probably damaging	Het
Srd5a1	T	C	13: 69,611,054 (GRCm38)	Y65C	probably benign	Het
Ssh2	T	A	11: 77,453,523 (GRCm38)	I778N	possibly damaging	Het
Sstr1	C	T	12: 58,213,386 (GRCm38)	S265L	probably damaging	Het
Syne2	T	A	12: 75,967,755 (GRCm38)		probably null	Het
Tctn1	A	T	5: 122,261,484 (GRCm38)	D92E	probably damaging	Het
Tiam2	A	G	17: 3,453,369 (GRCm38)	I940M	probably damaging	Het
Tmeff2	T	C	1: 50,979,440 (GRCm38)	W194R	unknown	Het
Tomm20	T	C	8: 126,937,153 (GRCm38)	T94A	probably benign	Het
Ucn2	A	G	9: 108,986,464 (GRCm38)	N98S	probably benign	Het
V1rd19	A	T	7: 24,003,318 (GRCm38)	I70L	probably benign	Het
Vmn1r18	A	G	6: 57,390,518 (GRCm38)	L17P	probably benign	Het
Vmn2r61	A	G	7: 42,260,110 (GRCm38)	T20A	probably benign	Het
Wdr59	G	A	8: 111,494,354 (GRCm38)	T182I		Het
Zfp40	A	T	17: 23,176,181 (GRCm38)	C477*	probably null	Het
Zfp68	A	G	5: 138,606,568 (GRCm38)	S498P	possibly damaging	Het
Zfp729b	T	C	13: 67,609,636 (GRCm38)		probably null	Het
Zfp846	T	G	9: 20,594,225 (GRCm38)	N460K	probably benign	Het
Zim1	T	C	7: 6,677,353 (GRCm38)	Y437C	probably damaging	Het

Other mutations in Mycbp2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00094:Mycbp2	APN	14	103,223,050 (GRCm38)	missense	probably damaging	1.00
IGL00518:Mycbp2	APN	14	103,155,808 (GRCm38)	missense	probably damaging	1.00
IGL00650:Mycbp2	APN	14	103,143,228 (GRCm38)	missense	probably damaging	0.97
IGL00653:Mycbp2	APN	14	103,143,228 (GRCm38)	missense	probably damaging	0.97
IGL00742:Mycbp2	APN	14	103,201,352 (GRCm38)	missense	probably damaging	1.00
IGL00755:Mycbp2	APN	14	103,194,621 (GRCm38)	missense	possibly damaging	0.72
IGL00793:Mycbp2	APN	14	103,126,753 (GRCm38)	missense	possibly damaging	0.77
IGL00916:Mycbp2	APN	14	103,291,283 (GRCm38)	splice site	probably benign
IGL00960:Mycbp2	APN	14	103,229,384 (GRCm38)	missense	possibly damaging	0.95
IGL00977:Mycbp2	APN	14	103,172,642 (GRCm38)	missense	probably damaging	0.98
IGL01349:Mycbp2	APN	14	103,122,547 (GRCm38)	missense	probably damaging	0.98
IGL01369:Mycbp2	APN	14	103,155,510 (GRCm38)	missense	possibly damaging	0.61
IGL01410:Mycbp2	APN	14	103,229,492 (GRCm38)	splice site	probably null
IGL01586:Mycbp2	APN	14	103,140,869 (GRCm38)	critical splice donor site	probably null
IGL01593:Mycbp2	APN	14	103,291,287 (GRCm38)	critical splice donor site	probably null
IGL01693:Mycbp2	APN	14	103,127,979 (GRCm38)	missense	probably damaging	0.99
IGL01730:Mycbp2	APN	14	103,135,204 (GRCm38)	nonsense	probably null
IGL01820:Mycbp2	APN	14	103,188,501 (GRCm38)	missense	probably damaging	1.00
IGL01974:Mycbp2	APN	14	103,143,211 (GRCm38)	missense	possibly damaging	0.88
IGL02071:Mycbp2	APN	14	103,154,907 (GRCm38)	nonsense	probably null
IGL02178:Mycbp2	APN	14	103,224,366 (GRCm38)	missense	probably benign	0.01
IGL02324:Mycbp2	APN	14	103,242,207 (GRCm38)	missense	probably damaging	1.00
IGL02442:Mycbp2	APN	14	103,314,375 (GRCm38)	missense	probably benign
IGL02607:Mycbp2	APN	14	103,285,273 (GRCm38)	missense	probably damaging	1.00
IGL02679:Mycbp2	APN	14	103,205,185 (GRCm38)	missense	probably benign
IGL02702:Mycbp2	APN	14	103,220,124 (GRCm38)	missense	probably benign	0.01
IGL02709:Mycbp2	APN	14	103,155,261 (GRCm38)	missense	probably damaging	0.97
IGL02736:Mycbp2	APN	14	103,114,242 (GRCm38)	splice site	probably benign
IGL02866:Mycbp2	APN	14	103,129,992 (GRCm38)	missense	probably damaging	0.98
IGL02939:Mycbp2	APN	14	103,177,279 (GRCm38)	missense	probably benign
IGL03082:Mycbp2	APN	14	103,204,369 (GRCm38)	missense	probably benign	0.23
IGL03142:Mycbp2	APN	14	103,298,776 (GRCm38)	missense	probably damaging	0.99
IGL03155:Mycbp2	APN	14	103,155,453 (GRCm38)	missense	probably benign	0.06
IGL03236:Mycbp2	APN	14	103,298,698 (GRCm38)	missense	probably damaging	0.99
IGL03256:Mycbp2	APN	14	103,188,589 (GRCm38)	missense	possibly damaging	0.92
IGL03303:Mycbp2	APN	14	103,247,758 (GRCm38)	missense	probably damaging	1.00
compost	UTSW	14	103,156,453 (GRCm38)	missense	probably damaging	1.00
decompose	UTSW	14	103,219,979 (GRCm38)	missense	probably benign	0.12
moulder	UTSW	14	103,188,592 (GRCm38)	missense	probably damaging	1.00
N/A - 293:Mycbp2	UTSW	14	103,224,462 (GRCm38)	splice site	probably benign
R0040:Mycbp2	UTSW	14	103,224,272 (GRCm38)	missense	probably benign	0.11
R0040:Mycbp2	UTSW	14	103,224,272 (GRCm38)	missense	probably benign	0.11
R0057:Mycbp2	UTSW	14	103,152,142 (GRCm38)	missense	probably damaging	0.97
R0063:Mycbp2	UTSW	14	103,156,634 (GRCm38)	unclassified	probably benign
R0097:Mycbp2	UTSW	14	103,155,762 (GRCm38)	missense	probably damaging	1.00
R0097:Mycbp2	UTSW	14	103,155,762 (GRCm38)	missense	probably damaging	1.00
R0268:Mycbp2	UTSW	14	103,314,325 (GRCm38)	nonsense	probably null
R0388:Mycbp2	UTSW	14	103,156,667 (GRCm38)	missense	probably benign	0.01
R0410:Mycbp2	UTSW	14	103,135,133 (GRCm38)	missense	probably damaging	1.00
R0530:Mycbp2	UTSW	14	103,182,459 (GRCm38)	missense	probably damaging	1.00
R0591:Mycbp2	UTSW	14	103,196,391 (GRCm38)	unclassified	probably benign
R0671:Mycbp2	UTSW	14	103,194,588 (GRCm38)	missense	possibly damaging	0.95
R0755:Mycbp2	UTSW	14	103,174,794 (GRCm38)	missense	probably damaging	1.00
R0817:Mycbp2	UTSW	14	103,229,418 (GRCm38)	missense	probably damaging	0.99
R0818:Mycbp2	UTSW	14	103,229,418 (GRCm38)	missense	probably damaging	0.99
R0819:Mycbp2	UTSW	14	103,229,418 (GRCm38)	missense	probably damaging	0.99
R0881:Mycbp2	UTSW	14	103,220,013 (GRCm38)	missense	probably benign
R0903:Mycbp2	UTSW	14	103,275,857 (GRCm38)	missense	probably damaging	0.99
R0940:Mycbp2	UTSW	14	103,262,693 (GRCm38)	unclassified	probably benign
R0961:Mycbp2	UTSW	14	103,184,835 (GRCm38)	missense	probably damaging	1.00
R1004:Mycbp2	UTSW	14	103,140,917 (GRCm38)	missense	probably benign	0.00
R1138:Mycbp2	UTSW	14	103,174,826 (GRCm38)	missense	possibly damaging	0.84
R1170:Mycbp2	UTSW	14	103,200,152 (GRCm38)	nonsense	probably null
R1211:Mycbp2	UTSW	14	103,120,563 (GRCm38)	missense	probably benign	0.31
R1268:Mycbp2	UTSW	14	103,208,782 (GRCm38)	missense	probably damaging	1.00
R1298:Mycbp2	UTSW	14	103,155,898 (GRCm38)	missense	probably damaging	1.00
R1341:Mycbp2	UTSW	14	103,298,867 (GRCm38)	splice site	probably benign
R1469:Mycbp2	UTSW	14	103,188,520 (GRCm38)	missense	probably damaging	0.99
R1469:Mycbp2	UTSW	14	103,188,520 (GRCm38)	missense	probably damaging	0.99
R1513:Mycbp2	UTSW	14	103,204,389 (GRCm38)	missense	probably damaging	1.00
R1528:Mycbp2	UTSW	14	103,232,597 (GRCm38)	missense	possibly damaging	0.91
R1564:Mycbp2	UTSW	14	103,169,851 (GRCm38)	splice site	probably null
R1565:Mycbp2	UTSW	14	103,252,509 (GRCm38)	missense	possibly damaging	0.82
R1656:Mycbp2	UTSW	14	103,247,758 (GRCm38)	missense	probably damaging	1.00
R1694:Mycbp2	UTSW	14	103,227,511 (GRCm38)	missense	probably damaging	1.00
R1709:Mycbp2	UTSW	14	103,224,416 (GRCm38)	missense	probably damaging	1.00
R1728:Mycbp2	UTSW	14	103,155,178 (GRCm38)	missense	probably damaging	0.98
R1751:Mycbp2	UTSW	14	103,248,405 (GRCm38)	missense	probably damaging	0.98
R1767:Mycbp2	UTSW	14	103,248,405 (GRCm38)	missense	probably damaging	0.98
R1772:Mycbp2	UTSW	14	103,182,419 (GRCm38)	missense	probably damaging	1.00
R1784:Mycbp2	UTSW	14	103,155,178 (GRCm38)	missense	probably damaging	0.98
R1823:Mycbp2	UTSW	14	103,252,509 (GRCm38)	missense	possibly damaging	0.82
R1824:Mycbp2	UTSW	14	103,252,509 (GRCm38)	missense	possibly damaging	0.82
R1844:Mycbp2	UTSW	14	103,155,714 (GRCm38)	missense	possibly damaging	0.94
R1916:Mycbp2	UTSW	14	103,184,883 (GRCm38)	missense	probably damaging	1.00
R1944:Mycbp2	UTSW	14	103,229,404 (GRCm38)	missense	probably damaging	1.00
R1983:Mycbp2	UTSW	14	103,145,971 (GRCm38)	missense	probably damaging	0.97
R2002:Mycbp2	UTSW	14	103,248,403 (GRCm38)	missense	probably damaging	0.98
R2031:Mycbp2	UTSW	14	103,188,592 (GRCm38)	missense	probably damaging	1.00
R2035:Mycbp2	UTSW	14	103,260,239 (GRCm38)	missense	probably damaging	1.00
R2048:Mycbp2	UTSW	14	103,232,524 (GRCm38)	critical splice donor site	probably null
R2061:Mycbp2	UTSW	14	103,287,260 (GRCm38)	missense	probably damaging	0.99
R2113:Mycbp2	UTSW	14	103,220,076 (GRCm38)	missense	probably damaging	0.99
R2128:Mycbp2	UTSW	14	103,201,230 (GRCm38)	missense	probably benign	0.01
R2134:Mycbp2	UTSW	14	103,208,893 (GRCm38)	missense	probably damaging	1.00
R2135:Mycbp2	UTSW	14	103,208,893 (GRCm38)	missense	probably damaging	1.00
R2135:Mycbp2	UTSW	14	103,145,942 (GRCm38)	missense	probably benign
R2146:Mycbp2	UTSW	14	103,155,922 (GRCm38)	missense	probably damaging	0.97
R2147:Mycbp2	UTSW	14	103,155,922 (GRCm38)	missense	probably damaging	0.97
R2148:Mycbp2	UTSW	14	103,155,922 (GRCm38)	missense	probably damaging	0.97
R2150:Mycbp2	UTSW	14	103,155,922 (GRCm38)	missense	probably damaging	0.97
R2163:Mycbp2	UTSW	14	103,169,855 (GRCm38)	critical splice donor site	probably null
R2248:Mycbp2	UTSW	14	103,169,859 (GRCm38)	missense	possibly damaging	0.50
R2265:Mycbp2	UTSW	14	103,262,749 (GRCm38)	missense	probably benign	0.39
R2272:Mycbp2	UTSW	14	103,144,338 (GRCm38)	missense	probably null	0.66
R2379:Mycbp2	UTSW	14	103,174,950 (GRCm38)	missense	probably benign
R2495:Mycbp2	UTSW	14	103,200,118 (GRCm38)	missense	probably damaging	0.99
R2508:Mycbp2	UTSW	14	103,131,245 (GRCm38)	missense	probably damaging	0.99
R2510:Mycbp2	UTSW	14	103,155,255 (GRCm38)	missense	probably damaging	0.96
R2851:Mycbp2	UTSW	14	103,144,333 (GRCm38)	missense	probably damaging	0.99
R2852:Mycbp2	UTSW	14	103,144,333 (GRCm38)	missense	probably damaging	0.99
R2965:Mycbp2	UTSW	14	103,297,358 (GRCm38)	missense	probably benign	0.00
R3156:Mycbp2	UTSW	14	103,208,743 (GRCm38)	splice site	probably benign
R3404:Mycbp2	UTSW	14	103,200,114 (GRCm38)	missense	probably damaging	0.99
R3410:Mycbp2	UTSW	14	103,135,117 (GRCm38)	missense	probably damaging	1.00
R3429:Mycbp2	UTSW	14	103,229,430 (GRCm38)	missense	probably damaging	1.00
R3706:Mycbp2	UTSW	14	103,156,414 (GRCm38)	missense	probably benign	0.31
R3772:Mycbp2	UTSW	14	103,133,788 (GRCm38)	missense	possibly damaging	0.82
R3778:Mycbp2	UTSW	14	103,197,285 (GRCm38)	missense	probably damaging	0.99
R3883:Mycbp2	UTSW	14	103,295,250 (GRCm38)	missense	probably damaging	0.97
R3884:Mycbp2	UTSW	14	103,295,250 (GRCm38)	missense	probably damaging	0.97
R3887:Mycbp2	UTSW	14	103,174,797 (GRCm38)	missense	probably damaging	0.98
R3923:Mycbp2	UTSW	14	103,126,713 (GRCm38)	missense	probably damaging	1.00
R3926:Mycbp2	UTSW	14	103,204,500 (GRCm38)	missense	probably damaging	1.00
R3959:Mycbp2	UTSW	14	103,295,252 (GRCm38)	missense	probably benign	0.00
R3966:Mycbp2	UTSW	14	103,138,725 (GRCm38)	splice site	probably benign
R4021:Mycbp2	UTSW	14	103,152,157 (GRCm38)	missense	probably damaging	0.97
R4363:Mycbp2	UTSW	14	103,248,457 (GRCm38)	missense	probably damaging	1.00
R4405:Mycbp2	UTSW	14	103,123,445 (GRCm38)	missense	probably damaging	1.00
R4407:Mycbp2	UTSW	14	103,287,228 (GRCm38)	missense	probably damaging	1.00
R4410:Mycbp2	UTSW	14	103,135,266 (GRCm38)	missense	probably damaging	1.00
R4434:Mycbp2	UTSW	14	103,133,789 (GRCm38)	missense	probably damaging	0.99
R4448:Mycbp2	UTSW	14	103,188,502 (GRCm38)	missense	possibly damaging	0.89
R4452:Mycbp2	UTSW	14	103,155,658 (GRCm38)	missense	probably damaging	0.99
R4573:Mycbp2	UTSW	14	103,346,297 (GRCm38)	missense	probably benign	0.05
R4589:Mycbp2	UTSW	14	103,177,313 (GRCm38)	missense	probably benign	0.04
R4621:Mycbp2	UTSW	14	103,219,979 (GRCm38)	missense	probably benign	0.12
R4622:Mycbp2	UTSW	14	103,219,979 (GRCm38)	missense	probably benign	0.12
R4729:Mycbp2	UTSW	14	103,188,591 (GRCm38)	missense	probably damaging	1.00
R4770:Mycbp2	UTSW	14	103,219,944 (GRCm38)	missense	probably benign	0.41
R4790:Mycbp2	UTSW	14	103,229,437 (GRCm38)	missense	probably damaging	1.00
R4884:Mycbp2	UTSW	14	103,211,295 (GRCm38)	missense	probably damaging	1.00
R4885:Mycbp2	UTSW	14	103,145,946 (GRCm38)	missense	possibly damaging	0.86
R4956:Mycbp2	UTSW	14	103,287,239 (GRCm38)	missense	probably damaging	0.99
R4980:Mycbp2	UTSW	14	103,260,385 (GRCm38)	splice site	probably null
R4994:Mycbp2	UTSW	14	103,169,994 (GRCm38)	missense	probably benign
R5029:Mycbp2	UTSW	14	103,156,510 (GRCm38)	missense	probably benign	0.21
R5038:Mycbp2	UTSW	14	103,296,939 (GRCm38)	missense	probably damaging	1.00
R5044:Mycbp2	UTSW	14	103,139,235 (GRCm38)	critical splice donor site	probably null
R5231:Mycbp2	UTSW	14	103,346,214 (GRCm38)	critical splice donor site	probably null
R5305:Mycbp2	UTSW	14	103,346,321 (GRCm38)	missense	probably benign	0.00
R5322:Mycbp2	UTSW	14	103,185,683 (GRCm38)	critical splice acceptor site	probably null
R5376:Mycbp2	UTSW	14	103,242,432 (GRCm38)	nonsense	probably null
R5414:Mycbp2	UTSW	14	103,306,261 (GRCm38)	missense	probably damaging	1.00
R5453:Mycbp2	UTSW	14	103,201,401 (GRCm38)	missense	probably damaging	0.99
R5462:Mycbp2	UTSW	14	103,200,126 (GRCm38)	missense	probably damaging	1.00
R5499:Mycbp2	UTSW	14	103,242,179 (GRCm38)	missense	probably damaging	1.00
R5502:Mycbp2	UTSW	14	103,173,814 (GRCm38)	missense	probably damaging	1.00
R5524:Mycbp2	UTSW	14	103,295,237 (GRCm38)	missense	probably damaging	1.00
R5533:Mycbp2	UTSW	14	103,282,645 (GRCm38)	nonsense	probably null
R5569:Mycbp2	UTSW	14	103,135,243 (GRCm38)	missense	probably damaging	1.00
R5574:Mycbp2	UTSW	14	103,142,767 (GRCm38)	missense	possibly damaging	0.94
R5579:Mycbp2	UTSW	14	103,291,333 (GRCm38)	missense	probably damaging	0.98
R5590:Mycbp2	UTSW	14	103,123,355 (GRCm38)	missense	probably damaging	1.00
R5592:Mycbp2	UTSW	14	103,194,677 (GRCm38)	missense	probably benign	0.02
R5643:Mycbp2	UTSW	14	103,287,334 (GRCm38)	missense	probably damaging	1.00
R5644:Mycbp2	UTSW	14	103,287,334 (GRCm38)	missense	probably damaging	1.00
R5645:Mycbp2	UTSW	14	103,188,615 (GRCm38)	critical splice acceptor site	probably null
R5645:Mycbp2	UTSW	14	103,188,608 (GRCm38)	missense	probably damaging	1.00
R5646:Mycbp2	UTSW	14	103,169,910 (GRCm38)	missense	probably benign	0.09
R5648:Mycbp2	UTSW	14	103,291,342 (GRCm38)	missense	probably damaging	1.00
R5651:Mycbp2	UTSW	14	103,282,665 (GRCm38)	missense	probably null	0.99
R5668:Mycbp2	UTSW	14	103,120,519 (GRCm38)	missense	possibly damaging	0.62
R5745:Mycbp2	UTSW	14	103,156,453 (GRCm38)	missense	possibly damaging	0.94
R5751:Mycbp2	UTSW	14	103,148,550 (GRCm38)	missense	probably damaging	0.99
R5756:Mycbp2	UTSW	14	103,133,974 (GRCm38)	missense	probably damaging	0.99
R5837:Mycbp2	UTSW	14	103,124,403 (GRCm38)	missense	probably damaging	1.00
R5984:Mycbp2	UTSW	14	103,126,684 (GRCm38)	missense	probably damaging	0.98
R6005:Mycbp2	UTSW	14	103,156,723 (GRCm38)	missense	probably benign
R6063:Mycbp2	UTSW	14	103,135,146 (GRCm38)	missense	probably damaging	1.00
R6091:Mycbp2	UTSW	14	103,223,046 (GRCm38)	missense	probably damaging	1.00
R6120:Mycbp2	UTSW	14	103,275,887 (GRCm38)	missense	probably benign	0.01
R6129:Mycbp2	UTSW	14	103,285,400 (GRCm38)	missense	probably benign	0.21
R6147:Mycbp2	UTSW	14	103,155,509 (GRCm38)	nonsense	probably null
R6161:Mycbp2	UTSW	14	103,298,747 (GRCm38)	missense	probably damaging	1.00
R6187:Mycbp2	UTSW	14	103,147,017 (GRCm38)	missense	probably damaging	1.00
R6208:Mycbp2	UTSW	14	103,295,228 (GRCm38)	missense	probably benign	0.11
R6228:Mycbp2	UTSW	14	103,260,229 (GRCm38)	missense	probably benign	0.24
R6301:Mycbp2	UTSW	14	103,155,426 (GRCm38)	missense	probably damaging	1.00
R6311:Mycbp2	UTSW	14	103,262,740 (GRCm38)	missense	possibly damaging	0.93
R6329:Mycbp2	UTSW	14	103,155,852 (GRCm38)	missense	probably benign	0.00
R6439:Mycbp2	UTSW	14	103,155,475 (GRCm38)	missense	probably benign	0.00
R6462:Mycbp2	UTSW	14	103,136,557 (GRCm38)	critical splice donor site	probably null
R6528:Mycbp2	UTSW	14	103,142,881 (GRCm38)	missense	probably damaging	0.99
R6736:Mycbp2	UTSW	14	103,191,567 (GRCm38)	missense	probably null	1.00
R6821:Mycbp2	UTSW	14	103,139,409 (GRCm38)	missense	probably damaging	1.00
R6851:Mycbp2	UTSW	14	103,260,194 (GRCm38)	critical splice donor site	probably null
R6948:Mycbp2	UTSW	14	103,285,267 (GRCm38)	missense	possibly damaging	0.94
R6977:Mycbp2	UTSW	14	103,154,906 (GRCm38)	missense	probably damaging	0.99
R6985:Mycbp2	UTSW	14	103,206,681 (GRCm38)	missense	possibly damaging	0.79
R7035:Mycbp2	UTSW	14	103,174,981 (GRCm38)	missense	probably benign
R7054:Mycbp2	UTSW	14	103,156,098 (GRCm38)	missense	possibly damaging	0.90
R7108:Mycbp2	UTSW	14	103,122,603 (GRCm38)	missense	probably damaging	1.00
R7117:Mycbp2	UTSW	14	103,154,077 (GRCm38)	missense	probably benign	0.21
R7137:Mycbp2	UTSW	14	103,282,679 (GRCm38)	missense	possibly damaging	0.94
R7169:Mycbp2	UTSW	14	103,260,200 (GRCm38)	missense	possibly damaging	0.78
R7218:Mycbp2	UTSW	14	103,133,846 (GRCm38)	missense	probably benign
R7234:Mycbp2	UTSW	14	103,215,337 (GRCm38)	missense	probably damaging	0.98
R7238:Mycbp2	UTSW	14	103,156,297 (GRCm38)	missense	probably damaging	1.00
R7244:Mycbp2	UTSW	14	103,208,909 (GRCm38)	missense	probably damaging	0.98
R7265:Mycbp2	UTSW	14	103,197,243 (GRCm38)	critical splice donor site	probably null
R7286:Mycbp2	UTSW	14	103,120,591 (GRCm38)	missense	probably damaging	1.00
R7332:Mycbp2	UTSW	14	103,156,453 (GRCm38)	missense	probably damaging	1.00
R7384:Mycbp2	UTSW	14	103,276,393 (GRCm38)	missense	probably damaging	0.99
R7392:Mycbp2	UTSW	14	103,243,128 (GRCm38)	missense	probably damaging	0.99
R7392:Mycbp2	UTSW	14	103,152,191 (GRCm38)	missense	probably damaging	1.00
R7409:Mycbp2	UTSW	14	103,288,744 (GRCm38)	missense	probably damaging	1.00
R7486:Mycbp2	UTSW	14	103,197,254 (GRCm38)	missense	probably damaging	0.97
R7643:Mycbp2	UTSW	14	103,346,265 (GRCm38)	missense	probably benign
R7661:Mycbp2	UTSW	14	103,212,623 (GRCm38)	missense	probably damaging	1.00
R7663:Mycbp2	UTSW	14	103,191,609 (GRCm38)	missense	probably damaging	0.99
R7730:Mycbp2	UTSW	14	103,123,355 (GRCm38)	missense	probably damaging	0.99
R7757:Mycbp2	UTSW	14	103,191,619 (GRCm38)	missense	probably damaging	1.00
R7773:Mycbp2	UTSW	14	103,248,404 (GRCm38)	missense	probably damaging	0.97
R7787:Mycbp2	UTSW	14	103,127,097 (GRCm38)	missense	probably damaging	1.00
R7822:Mycbp2	UTSW	14	103,139,415 (GRCm38)	missense	probably benign	0.00
R7838:Mycbp2	UTSW	14	103,177,293 (GRCm38)	missense	probably benign	0.10
R7841:Mycbp2	UTSW	14	103,146,831 (GRCm38)	critical splice donor site	probably null
R7858:Mycbp2	UTSW	14	103,156,305 (GRCm38)	missense	probably damaging	1.00
R7873:Mycbp2	UTSW	14	103,156,146 (GRCm38)	missense	probably damaging	1.00
R7911:Mycbp2	UTSW	14	103,200,185 (GRCm38)	missense	probably damaging	0.99
R7942:Mycbp2	UTSW	14	103,155,238 (GRCm38)	missense	probably damaging	0.99
R7951:Mycbp2	UTSW	14	103,215,462 (GRCm38)	missense	probably damaging	0.99
R7958:Mycbp2	UTSW	14	103,129,964 (GRCm38)	missense	probably benign	0.00
R8235:Mycbp2	UTSW	14	103,198,674 (GRCm38)	missense	probably damaging	0.99
R8246:Mycbp2	UTSW	14	103,155,204 (GRCm38)	missense	probably damaging	0.99
R8338:Mycbp2	UTSW	14	103,135,265 (GRCm38)	missense	probably damaging	1.00
R8343:Mycbp2	UTSW	14	103,160,675 (GRCm38)	splice site	probably null
R8361:Mycbp2	UTSW	14	103,138,814 (GRCm38)	missense	probably damaging	1.00
R8490:Mycbp2	UTSW	14	103,208,831 (GRCm38)	missense	probably benign	0.00
R8524:Mycbp2	UTSW	14	103,155,459 (GRCm38)	missense	probably benign	0.23
R8525:Mycbp2	UTSW	14	103,212,719 (GRCm38)	missense	probably damaging	1.00
R8711:Mycbp2	UTSW	14	103,169,994 (GRCm38)	missense	probably benign	0.08
R8735:Mycbp2	UTSW	14	103,223,150 (GRCm38)	missense	probably damaging	0.99
R8825:Mycbp2	UTSW	14	103,229,435 (GRCm38)	missense	probably damaging	1.00
R8928:Mycbp2	UTSW	14	103,156,345 (GRCm38)	missense	probably benign
R8974:Mycbp2	UTSW	14	103,124,421 (GRCm38)	missense	probably damaging	1.00
R8987:Mycbp2	UTSW	14	103,208,796 (GRCm38)	missense	probably damaging	1.00
R9021:Mycbp2	UTSW	14	103,314,316 (GRCm38)	missense	probably benign	0.08
R9062:Mycbp2	UTSW	14	103,242,360 (GRCm38)	missense	probably benign	0.00
R9077:Mycbp2	UTSW	14	103,232,538 (GRCm38)	missense	probably damaging	1.00
R9208:Mycbp2	UTSW	14	103,295,228 (GRCm38)	missense	probably benign	0.01
R9285:Mycbp2	UTSW	14	103,197,317 (GRCm38)	missense	probably damaging	0.97
R9290:Mycbp2	UTSW	14	103,188,524 (GRCm38)	missense	probably damaging	0.99
R9362:Mycbp2	UTSW	14	103,260,206 (GRCm38)	missense	probably damaging	0.97
R9520:Mycbp2	UTSW	14	103,260,269 (GRCm38)	missense	probably benign	0.02
R9557:Mycbp2	UTSW	14	103,135,261 (GRCm38)	missense	probably benign	0.03
R9639:Mycbp2	UTSW	14	103,196,381 (GRCm38)	missense	probably damaging	1.00
R9666:Mycbp2	UTSW	14	103,134,038 (GRCm38)	missense	probably damaging	0.98
R9732:Mycbp2	UTSW	14	103,211,313 (GRCm38)	missense	probably damaging	1.00
R9736:Mycbp2	UTSW	14	103,197,416 (GRCm38)	missense	probably damaging	0.96
R9739:Mycbp2	UTSW	14	103,282,793 (GRCm38)	missense	probably benign	0.11
R9755:Mycbp2	UTSW	14	103,314,370 (GRCm38)	missense	probably benign
X0024:Mycbp2	UTSW	14	103,146,942 (GRCm38)	missense	probably damaging	1.00
Z1176:Mycbp2	UTSW	14	103,346,249 (GRCm38)	missense	probably benign
Z1176:Mycbp2	UTSW	14	103,156,637 (GRCm38)	missense	probably benign	0.06
Z1177:Mycbp2	UTSW	14	103,169,873 (GRCm38)	missense	possibly damaging	0.83
Z1177:Mycbp2	UTSW	14	103,135,123 (GRCm38)	missense	probably damaging	1.00
Z1177:Mycbp2	UTSW	14	103,127,063 (GRCm38)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- TAACACGAAAATGGCCATTTCC -3'
(R):5'- TTGCACACATATTTAACCTCTGCAC -3'

Sequencing Primer
(F):5'- AAATGGCCATTTCCTCCTTTAATTTC -3'
(R):5'- TATTTAACCTCTGCACTCAAAAGGC -3'

Posted On

2019-09-13