Mutagenetix > Incidental Mutation

Incidental Mutation 'R7888:Vmn2r4'

609150

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r4

Ensembl Gene

ENSMUSG00000092049

Gene Name

vomeronasal 2, receptor 4

Synonyms

EG637053

MMRRC Submission

045940-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R7888 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64295982-64322741 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 64313943 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 346 (E346G)

Ref Sequence

ENSEMBL: ENSMUSP00000135228 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170280] [ENSMUST00000175724]

AlphaFold

K7N784

Predicted Effect

probably damaging
Transcript: ENSMUST00000170280
AA Change: E257G

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000127513
Gene: ENSMUSG00000092049
AA Change: E257G

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	2.7e-72	PFAM
Pfam:Peripla_BP_6	61	240	1.9e-9	PFAM
Pfam:NCD3G	458	511	1.1e-17	PFAM
Pfam:7tm_3	542	779	1.8e-75	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000175724
AA Change: E346G

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000135228
Gene: ENSMUSG00000092049
AA Change: E346G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	2.3e-75	PFAM
Pfam:NCD3G	547	600	4.7e-17	PFAM
Pfam:7tm_3	633	867	8.2e-47	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (45/45)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox2	G	T	14: 8,246,415 (GRCm38)	Q459K	probably benign	Het
Ano3	T	A	2: 110,496,773 (GRCm39)	Y792F	probably damaging	Het
Aoc3	C	T	11: 101,223,323 (GRCm39)	H520Y	probably damaging	Het
Atp4b	A	G	8: 13,439,811 (GRCm39)	F137S	probably damaging	Het
Blvrb	A	G	7: 27,165,159 (GRCm39)	T160A	probably damaging	Het
Brd2	G	A	17: 34,335,995 (GRCm39)	R73W	probably damaging	Het
Btaf1	A	G	19: 36,943,036 (GRCm39)	T306A	probably benign	Het
Ccdc154	G	A	17: 25,383,578 (GRCm39)	V212M	possibly damaging	Het
Ccdc40	A	G	11: 119,119,967 (GRCm39)	E3G	unknown	Het
Cenpb	T	A	2: 131,021,762 (GRCm39)	E12V	probably damaging	Het
Cnot8	T	C	11: 58,002,137 (GRCm39)	S57P	probably benign	Het
Cryba4	T	C	5: 112,398,918 (GRCm39)	E42G	probably benign	Het
Fam72a	T	A	1: 131,456,578 (GRCm39)	I47N	probably damaging	Het
Gm27027	A	C	2: 93,787,880 (GRCm39)		probably null	Het
Itgb2	T	C	10: 77,400,478 (GRCm39)	V697A	probably benign	Het
Jakmip1	A	G	5: 37,262,208 (GRCm39)	N336D	probably damaging	Het
Kansl1	T	C	11: 104,233,248 (GRCm39)	T760A	probably benign	Het
Lrrc37a	T	A	11: 103,392,307 (GRCm39)	E1039D	probably benign	Het
Meaf6	T	G	4: 125,003,213 (GRCm39)		probably null	Het
Mpz	T	C	1: 170,987,204 (GRCm39)		probably null	Het
Mtss1	A	T	15: 58,844,373 (GRCm39)	M82K	probably damaging	Het
Myo6	T	C	9: 80,203,947 (GRCm39)	S1063P	probably damaging	Het
Niban2	T	A	2: 32,812,137 (GRCm39)	Y406*	probably null	Het
Nsun6	T	A	2: 15,001,355 (GRCm39)	E400D	probably benign	Het
Or10al5	A	T	17: 38,062,888 (GRCm39)	N48Y	probably damaging	Het
Or1e26	T	C	11: 73,480,354 (GRCm39)	D70G	probably damaging	Het
Or1l4b	A	T	2: 37,036,334 (GRCm39)	M37L	probably benign	Het
Or2t26	T	C	11: 49,039,266 (GRCm39)	Y61H	probably damaging	Het
Or52ae7	T	A	7: 103,120,006 (GRCm39)	Y253*	probably null	Het
Or5au1	A	G	14: 52,273,347 (GRCm39)	S74P	probably damaging	Het
Or8j3b	C	A	2: 86,205,270 (GRCm39)	C162F	probably benign	Het
Pfdn5	T	A	15: 102,237,024 (GRCm39)	V92E	probably damaging	Het
Pik3c2g	T	C	6: 139,842,470 (GRCm39)	V801A		Het
Psme2b	T	A	11: 48,836,402 (GRCm39)	T182S	possibly damaging	Het
Ptcd3	G	A	6: 71,860,431 (GRCm39)	A592V	probably damaging	Het
Rabgap1	T	A	2: 37,427,319 (GRCm39)	Y633*	probably null	Het
Rnf39	A	T	17: 37,258,133 (GRCm39)	T222S	probably damaging	Het
Scnn1g	A	G	7: 121,342,878 (GRCm39)	N277S	probably damaging	Het
Slc16a13	T	G	11: 70,109,806 (GRCm39)	I232L	possibly damaging	Het
Slco2b1	T	G	7: 99,338,050 (GRCm39)	R111S	unknown	Het
Sltm	C	G	9: 70,493,955 (GRCm39)	P802R	possibly damaging	Het
Tmc3	T	C	7: 83,249,217 (GRCm39)	W269R	probably damaging	Het
Vps52	A	G	17: 34,184,725 (GRCm39)	N666S	probably damaging	Het
Wdfy4	C	T	14: 32,812,920 (GRCm39)	D1618N		Het
Zfp773	C	A	7: 7,135,978 (GRCm39)	C206F	probably benign	Het

Other mutations in Vmn2r4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01310:Vmn2r4	APN	3	64,317,200 (GRCm39)	splice site	probably null
IGL01448:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01452:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01454:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01456:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01463:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01467:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01468:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01470:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01476:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01481:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01534:Vmn2r4	APN	3	64,313,844 (GRCm39)	missense	probably damaging	1.00
IGL01636:Vmn2r4	APN	3	64,313,657 (GRCm39)	missense	probably benign	0.21
IGL01879:Vmn2r4	APN	3	64,298,431 (GRCm39)	missense	probably damaging	1.00
IGL02147:Vmn2r4	APN	3	64,305,782 (GRCm39)	splice site	probably benign
IGL02276:Vmn2r4	APN	3	64,313,877 (GRCm39)	missense	possibly damaging	0.95
IGL02432:Vmn2r4	APN	3	64,313,821 (GRCm39)	missense	probably benign	0.38
IGL02533:Vmn2r4	APN	3	64,305,840 (GRCm39)	nonsense	probably null
IGL02655:Vmn2r4	APN	3	64,305,886 (GRCm39)	missense	probably damaging	0.97
IGL02666:Vmn2r4	APN	3	64,296,433 (GRCm39)	missense	probably benign	0.10
IGL02902:Vmn2r4	APN	3	64,314,337 (GRCm39)	missense	probably benign	0.22
IGL03189:Vmn2r4	APN	3	64,296,589 (GRCm39)	missense	possibly damaging	0.89
IGL03250:Vmn2r4	APN	3	64,314,063 (GRCm39)	missense	probably damaging	1.00
IGL03271:Vmn2r4	APN	3	64,305,850 (GRCm39)	missense	probably benign	0.01
R0310:Vmn2r4	UTSW	3	64,296,855 (GRCm39)	nonsense	probably null
R0504:Vmn2r4	UTSW	3	64,296,784 (GRCm39)	missense	probably damaging	1.00
R1546:Vmn2r4	UTSW	3	64,314,309 (GRCm39)	missense	probably damaging	1.00
R1562:Vmn2r4	UTSW	3	64,296,865 (GRCm39)	missense	probably damaging	0.98
R1863:Vmn2r4	UTSW	3	64,314,410 (GRCm39)	missense	probably benign	0.33
R1873:Vmn2r4	UTSW	3	64,298,479 (GRCm39)	missense	possibly damaging	0.93
R1939:Vmn2r4	UTSW	3	64,305,976 (GRCm39)	missense	probably benign	0.00
R2103:Vmn2r4	UTSW	3	64,322,704 (GRCm39)	missense	possibly damaging	0.48
R3083:Vmn2r4	UTSW	3	64,296,788 (GRCm39)	missense	probably damaging	1.00
R3687:Vmn2r4	UTSW	3	64,296,896 (GRCm39)	missense	possibly damaging	0.93
R3707:Vmn2r4	UTSW	3	64,296,895 (GRCm39)	missense	probably damaging	0.99
R3963:Vmn2r4	UTSW	3	64,322,572 (GRCm39)	missense	probably damaging	0.99
R4428:Vmn2r4	UTSW	3	64,322,590 (GRCm39)	missense	probably damaging	1.00
R4710:Vmn2r4	UTSW	3	64,317,201 (GRCm39)	critical splice donor site	probably null
R4737:Vmn2r4	UTSW	3	64,317,384 (GRCm39)	missense	probably damaging	1.00
R4767:Vmn2r4	UTSW	3	64,298,397 (GRCm39)	missense	probably damaging	0.99
R4776:Vmn2r4	UTSW	3	64,296,082 (GRCm39)	missense	probably damaging	0.96
R4834:Vmn2r4	UTSW	3	64,317,484 (GRCm39)	missense	probably benign	0.40
R4893:Vmn2r4	UTSW	3	64,313,676 (GRCm39)	missense	probably damaging	0.96
R4908:Vmn2r4	UTSW	3	64,296,476 (GRCm39)	missense	possibly damaging	0.59
R5049:Vmn2r4	UTSW	3	64,306,019 (GRCm39)	splice site	probably null
R5092:Vmn2r4	UTSW	3	64,298,373 (GRCm39)	missense	probably benign	0.01
R5234:Vmn2r4	UTSW	3	64,305,878 (GRCm39)	missense	possibly damaging	0.88
R5240:Vmn2r4	UTSW	3	64,314,358 (GRCm39)	missense	possibly damaging	0.53
R5704:Vmn2r4	UTSW	3	64,317,370 (GRCm39)	missense	probably benign	0.03
R5897:Vmn2r4	UTSW	3	64,322,687 (GRCm39)	nonsense	probably null
R5907:Vmn2r4	UTSW	3	64,298,487 (GRCm39)	missense	probably damaging	0.99
R5924:Vmn2r4	UTSW	3	64,296,685 (GRCm39)	missense	probably damaging	1.00
R6145:Vmn2r4	UTSW	3	64,314,364 (GRCm39)	missense	probably benign	0.00
R6191:Vmn2r4	UTSW	3	64,322,702 (GRCm39)	missense	probably benign	0.34
R6192:Vmn2r4	UTSW	3	64,322,699 (GRCm39)	missense	probably benign	0.00
R6207:Vmn2r4	UTSW	3	64,313,926 (GRCm39)	missense	probably damaging	1.00
R6457:Vmn2r4	UTSW	3	64,317,378 (GRCm39)	missense	probably damaging	1.00
R6533:Vmn2r4	UTSW	3	64,322,519 (GRCm39)	missense	probably benign
R6545:Vmn2r4	UTSW	3	64,313,777 (GRCm39)	missense	possibly damaging	0.50
R6594:Vmn2r4	UTSW	3	64,296,731 (GRCm39)	missense	probably damaging	1.00
R7049:Vmn2r4	UTSW	3	64,296,550 (GRCm39)	missense	probably benign	0.14
R7150:Vmn2r4	UTSW	3	64,305,898 (GRCm39)	missense	probably benign	0.01
R7187:Vmn2r4	UTSW	3	64,322,681 (GRCm39)	missense	probably benign	0.00
R7363:Vmn2r4	UTSW	3	64,314,432 (GRCm39)	missense	probably damaging	1.00
R7477:Vmn2r4	UTSW	3	64,305,850 (GRCm39)	missense	probably benign	0.01
R7675:Vmn2r4	UTSW	3	64,322,657 (GRCm39)	missense	probably benign	0.01
R7858:Vmn2r4	UTSW	3	64,317,226 (GRCm39)	missense	probably benign	0.00
R8678:Vmn2r4	UTSW	3	64,314,391 (GRCm39)	missense	probably benign
R8743:Vmn2r4	UTSW	3	64,317,247 (GRCm39)	missense	possibly damaging	0.95
R8841:Vmn2r4	UTSW	3	64,314,058 (GRCm39)	missense	probably damaging	0.97
R9671:Vmn2r4	UTSW	3	64,317,271 (GRCm39)	missense	probably benign	0.00
R9778:Vmn2r4	UTSW	3	64,322,497 (GRCm39)	missense	probably benign	0.15
X0019:Vmn2r4	UTSW	3	64,314,057 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CAGTGCAGAGCTGATCAGAC -3'
(R):5'- GTAAAGAGTTCCACTGCCAAAGTC -3'

Sequencing Primer
(F):5'- GCTGATCAGACATGTCATACAGTCTG -3'
(R):5'- GAGTTCCACTGCCAAAGTCATTGTG -3'

Posted On

2019-12-20