Incidental Mutation 'R2030:Atp13a4'
| ID |
221183 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Atp13a4
|
| Ensembl Gene |
ENSMUSG00000038094 |
| Gene Name |
ATPase type 13A4 |
| Synonyms |
9330174J19Rik, 4631413J11Rik |
| MMRRC Submission |
040037-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R2030 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
16 |
| Chromosomal Location |
29395853-29544864 bp(-) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 29422684 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Aspartic acid
at position 741
(V741D)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000048753
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000039090]
[ENSMUST00000057018]
[ENSMUST00000182013]
[ENSMUST00000182627]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000039090
AA Change: V741D
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000048753
Gene: ENSMUSG00000038094
AA Change: V741D
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:P5-ATPase
|
17 |
143 |
8.4e-31 |
PFAM |
|
Cation_ATPase_N
|
147 |
223 |
1.09e-1 |
SMART |
|
Pfam:E1-E2_ATPase
|
229 |
476 |
1.7e-36 |
PFAM |
|
Pfam:Hydrolase
|
481 |
769 |
3.9e-11 |
PFAM |
|
Pfam:HAD
|
484 |
787 |
4.1e-14 |
PFAM |
|
Pfam:Hydrolase_like2
|
574 |
637 |
1.2e-9 |
PFAM |
|
transmembrane domain
|
824 |
846 |
N/A |
INTRINSIC |
|
| Predicted Effect |
unknown
Transcript: ENSMUST00000057018
AA Change: V722D
|
| SMART Domains |
Protein: ENSMUSP00000060987
Gene: ENSMUSG00000038094
AA Change: V722D
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:P5-ATPase
|
17 |
142 |
9.6e-34 |
PFAM |
|
Cation_ATPase_N
|
147 |
223 |
1.09e-1 |
SMART |
|
Pfam:E1-E2_ATPase
|
228 |
476 |
1.6e-34 |
PFAM |
|
Pfam:Hydrolase
|
481 |
767 |
1.1e-10 |
PFAM |
|
Pfam:HAD
|
484 |
858 |
3.3e-23 |
PFAM |
|
Pfam:Cation_ATPase
|
573 |
637 |
4.9e-8 |
PFAM |
|
transmembrane domain
|
902 |
924 |
N/A |
INTRINSIC |
|
transmembrane domain
|
934 |
951 |
N/A |
INTRINSIC |
|
transmembrane domain
|
972 |
994 |
N/A |
INTRINSIC |
|
low complexity region
|
1014 |
1023 |
N/A |
INTRINSIC |
|
transmembrane domain
|
1040 |
1057 |
N/A |
INTRINSIC |
|
transmembrane domain
|
1070 |
1092 |
N/A |
INTRINSIC |
|
transmembrane domain
|
1107 |
1126 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000182013
|
| SMART Domains |
Protein: ENSMUSP00000138583
Gene: ENSMUSG00000038094
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:P5-ATPase
|
17 |
84 |
4.2e-26 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000182168
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000182357
|
| Predicted Effect |
unknown
Transcript: ENSMUST00000182573
AA Change: V379D
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000182627
AA Change: V741D
PolyPhen 2
Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000138479
Gene: ENSMUSG00000038094
AA Change: V741D
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:P5-ATPase
|
17 |
143 |
2.1e-29 |
PFAM |
|
Cation_ATPase_N
|
147 |
223 |
1.09e-1 |
SMART |
|
Pfam:E1-E2_ATPase
|
229 |
476 |
3.9e-35 |
PFAM |
|
Pfam:Hydrolase
|
481 |
861 |
4.2e-16 |
PFAM |
|
Pfam:HAD
|
484 |
858 |
1.9e-23 |
PFAM |
|
Pfam:Hydrolase_like2
|
574 |
637 |
2.2e-8 |
PFAM |
|
transmembrane domain
|
902 |
924 |
N/A |
INTRINSIC |
|
transmembrane domain
|
934 |
951 |
N/A |
INTRINSIC |
|
transmembrane domain
|
972 |
994 |
N/A |
INTRINSIC |
|
low complexity region
|
1014 |
1023 |
N/A |
INTRINSIC |
|
transmembrane domain
|
1040 |
1057 |
N/A |
INTRINSIC |
|
transmembrane domain
|
1070 |
1092 |
N/A |
INTRINSIC |
|
transmembrane domain
|
1107 |
1126 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.7825 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 97.0%
- 20x: 94.2%
|
| Validation Efficiency |
100% (54/54) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 53 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700022I11Rik |
C |
T |
4: 42,974,131 (GRCm38) |
Q1155* |
probably null |
Het |
| Adtrp |
C |
T |
13: 41,828,259 (GRCm38) |
V13I |
probably damaging |
Het |
| Ak2 |
A |
G |
4: 129,008,220 (GRCm38) |
K229E |
probably benign |
Het |
| Bdp1 |
A |
T |
13: 100,061,189 (GRCm38) |
M896K |
probably benign |
Het |
| Ccser1 |
C |
T |
6: 61,311,563 (GRCm38) |
R237C |
probably benign |
Het |
| Cdk7 |
A |
G |
13: 100,722,674 (GRCm38) |
|
probably benign |
Het |
| CK137956 |
A |
G |
4: 127,951,387 (GRCm38) |
S188P |
probably benign |
Het |
| Dab1 |
C |
T |
4: 104,731,751 (GRCm38) |
A524V |
probably benign |
Het |
| Dbndd2 |
A |
G |
2: 164,488,643 (GRCm38) |
D72G |
probably damaging |
Het |
| Disp3 |
A |
T |
4: 148,259,966 (GRCm38) |
I493N |
probably damaging |
Het |
| Epx |
T |
C |
11: 87,864,824 (GRCm38) |
D678G |
probably damaging |
Het |
| Fbxw5 |
G |
T |
2: 25,504,798 (GRCm38) |
V235L |
probably damaging |
Het |
| Fmo4 |
T |
A |
1: 162,794,172 (GRCm38) |
D490V |
probably damaging |
Het |
| Fuca2 |
A |
G |
10: 13,506,774 (GRCm38) |
Y268C |
probably damaging |
Het |
| Gm4787 |
C |
T |
12: 81,378,770 (GRCm38) |
V205I |
probably damaging |
Het |
| Gpat3 |
G |
A |
5: 100,897,821 (GRCm38) |
R437K |
probably benign |
Het |
| Herc2 |
A |
G |
7: 56,184,373 (GRCm38) |
S3109G |
probably damaging |
Het |
| Hr |
G |
A |
14: 70,571,448 (GRCm38) |
R1117H |
probably damaging |
Het |
| Htra4 |
T |
A |
8: 25,033,577 (GRCm38) |
D324V |
probably damaging |
Het |
| Kcnd3 |
A |
G |
3: 105,459,537 (GRCm38) |
Y241C |
probably damaging |
Het |
| Kcp |
G |
A |
6: 29,489,072 (GRCm38) |
L1072F |
probably damaging |
Het |
| Lrrc52 |
T |
C |
1: 167,466,459 (GRCm38) |
N86D |
probably benign |
Het |
| Mindy4 |
A |
G |
6: 55,211,262 (GRCm38) |
T26A |
probably damaging |
Het |
| Mre11a |
A |
T |
9: 14,795,805 (GRCm38) |
N117Y |
probably damaging |
Het |
| Mrgprb1 |
A |
T |
7: 48,447,328 (GRCm38) |
S279T |
possibly damaging |
Het |
| Myh13 |
T |
G |
11: 67,350,238 (GRCm38) |
S814A |
probably benign |
Het |
| Ncapd2 |
A |
G |
6: 125,176,715 (GRCm38) |
V679A |
possibly damaging |
Het |
| Nipbl |
A |
T |
15: 8,350,287 (GRCm38) |
V1007D |
probably damaging |
Het |
| Nlrp12 |
T |
A |
7: 3,228,417 (GRCm38) |
H960L |
probably damaging |
Het |
| Olfr1245 |
C |
T |
2: 89,575,214 (GRCm38) |
V171I |
probably benign |
Het |
| Olfr402 |
T |
A |
11: 74,155,943 (GRCm38) |
M263K |
possibly damaging |
Het |
| Olfr860 |
A |
G |
9: 19,846,413 (GRCm38) |
S69P |
probably benign |
Het |
| Pklr |
A |
G |
3: 89,143,238 (GRCm38) |
Y402C |
probably damaging |
Het |
| Prdm2 |
T |
C |
4: 143,132,764 (GRCm38) |
T1319A |
possibly damaging |
Het |
| Rif1 |
T |
A |
2: 52,092,346 (GRCm38) |
V541E |
probably damaging |
Het |
| Ryk |
T |
A |
9: 102,881,656 (GRCm38) |
I248N |
possibly damaging |
Het |
| Shisa2 |
A |
T |
14: 59,629,685 (GRCm38) |
I129F |
probably damaging |
Het |
| Snap25 |
T |
G |
2: 136,770,053 (GRCm38) |
|
probably benign |
Het |
| Snx31 |
G |
A |
15: 36,525,702 (GRCm38) |
P284S |
probably benign |
Het |
| Tas2r137 |
A |
G |
6: 40,492,220 (GRCm38) |
K328R |
possibly damaging |
Het |
| Tas2r140 |
T |
C |
6: 133,055,250 (GRCm38) |
T182A |
probably benign |
Het |
| Thumpd2 |
G |
A |
17: 81,064,958 (GRCm38) |
R35C |
probably damaging |
Het |
| Tnxb |
A |
T |
17: 34,718,469 (GRCm38) |
N3811Y |
probably damaging |
Het |
| Tpte |
A |
C |
8: 22,345,885 (GRCm38) |
N428T |
probably damaging |
Het |
| Trpm6 |
A |
G |
19: 18,854,265 (GRCm38) |
D1498G |
probably benign |
Het |
| Tsc2 |
C |
T |
17: 24,623,470 (GRCm38) |
|
probably benign |
Het |
| Ttn |
C |
T |
2: 76,872,933 (GRCm38) |
|
probably benign |
Het |
| Wdr24 |
A |
G |
17: 25,826,043 (GRCm38) |
I251V |
probably benign |
Het |
| Wdr92 |
A |
T |
11: 17,229,832 (GRCm38) |
T278S |
probably benign |
Het |
| Zc3h6 |
T |
C |
2: 129,006,086 (GRCm38) |
Y278H |
probably damaging |
Het |
| Zfat |
A |
G |
15: 68,118,934 (GRCm38) |
|
probably null |
Het |
| Zfp442 |
A |
T |
2: 150,408,122 (GRCm38) |
V620E |
possibly damaging |
Het |
| Zfp788 |
A |
G |
7: 41,649,560 (GRCm38) |
H540R |
probably damaging |
Het |
|
| Other mutations in Atp13a4 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00563:Atp13a4
|
APN |
16 |
29,403,778 (GRCm38) |
splice site |
probably benign |
|
|
IGL01577:Atp13a4
|
APN |
16 |
29,441,284 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
IGL01834:Atp13a4
|
APN |
16 |
29,415,777 (GRCm38) |
splice site |
probably benign |
|
|
IGL02165:Atp13a4
|
APN |
16 |
29,434,010 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02194:Atp13a4
|
APN |
16 |
29,456,629 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02322:Atp13a4
|
APN |
16 |
29,440,102 (GRCm38) |
missense |
probably benign |
0.00 |
|
IGL02553:Atp13a4
|
APN |
16 |
29,422,703 (GRCm38) |
missense |
probably benign |
0.03 |
|
IGL02821:Atp13a4
|
APN |
16 |
29,441,307 (GRCm38) |
missense |
probably benign |
0.01 |
|
IGL03349:Atp13a4
|
APN |
16 |
29,456,671 (GRCm38) |
missense |
probably benign |
0.01 |
|
G5030:Atp13a4
|
UTSW |
16 |
29,455,488 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0091:Atp13a4
|
UTSW |
16 |
29,455,395 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0100:Atp13a4
|
UTSW |
16 |
29,421,724 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0278:Atp13a4
|
UTSW |
16 |
29,454,834 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1263:Atp13a4
|
UTSW |
16 |
29,471,953 (GRCm38) |
missense |
possibly damaging |
0.60 |
|
R1378:Atp13a4
|
UTSW |
16 |
29,420,428 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1575:Atp13a4
|
UTSW |
16 |
29,409,710 (GRCm38) |
missense |
probably benign |
0.01 |
|
R1720:Atp13a4
|
UTSW |
16 |
29,408,928 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R1759:Atp13a4
|
UTSW |
16 |
29,456,611 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R1967:Atp13a4
|
UTSW |
16 |
29,479,854 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R2113:Atp13a4
|
UTSW |
16 |
29,441,284 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R3409:Atp13a4
|
UTSW |
16 |
29,413,749 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3410:Atp13a4
|
UTSW |
16 |
29,413,749 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4032:Atp13a4
|
UTSW |
16 |
29,418,571 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4163:Atp13a4
|
UTSW |
16 |
29,541,250 (GRCm38) |
missense |
possibly damaging |
0.87 |
|
R4652:Atp13a4
|
UTSW |
16 |
29,452,603 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4772:Atp13a4
|
UTSW |
16 |
29,420,835 (GRCm38) |
intron |
probably benign |
|
|
R4795:Atp13a4
|
UTSW |
16 |
29,490,008 (GRCm38) |
critical splice donor site |
probably null |
|
|
R4898:Atp13a4
|
UTSW |
16 |
29,408,961 (GRCm38) |
nonsense |
probably null |
|
|
R4996:Atp13a4
|
UTSW |
16 |
29,472,004 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5112:Atp13a4
|
UTSW |
16 |
29,409,868 (GRCm38) |
missense |
possibly damaging |
0.87 |
|
R5259:Atp13a4
|
UTSW |
16 |
29,456,610 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5395:Atp13a4
|
UTSW |
16 |
29,456,604 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R5395:Atp13a4
|
UTSW |
16 |
29,420,888 (GRCm38) |
nonsense |
probably null |
|
|
R5640:Atp13a4
|
UTSW |
16 |
29,415,831 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R5809:Atp13a4
|
UTSW |
16 |
29,433,987 (GRCm38) |
missense |
possibly damaging |
0.56 |
|
R5856:Atp13a4
|
UTSW |
16 |
29,433,987 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R5912:Atp13a4
|
UTSW |
16 |
29,456,571 (GRCm38) |
missense |
probably benign |
0.33 |
|
R6282:Atp13a4
|
UTSW |
16 |
29,434,004 (GRCm38) |
missense |
probably benign |
0.00 |
|
R6404:Atp13a4
|
UTSW |
16 |
29,471,901 (GRCm38) |
nonsense |
probably null |
|
|
R6497:Atp13a4
|
UTSW |
16 |
29,479,901 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6577:Atp13a4
|
UTSW |
16 |
29,479,841 (GRCm38) |
missense |
probably benign |
0.03 |
|
R6806:Atp13a4
|
UTSW |
16 |
29,469,280 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7229:Atp13a4
|
UTSW |
16 |
29,420,905 (GRCm38) |
missense |
probably benign |
0.05 |
|
R7438:Atp13a4
|
UTSW |
16 |
29,441,196 (GRCm38) |
missense |
|
|
|
R7493:Atp13a4
|
UTSW |
16 |
29,471,956 (GRCm38) |
missense |
|
|
|
R7712:Atp13a4
|
UTSW |
16 |
29,459,487 (GRCm38) |
missense |
|
|
|
R7739:Atp13a4
|
UTSW |
16 |
29,456,601 (GRCm38) |
missense |
|
|
|
R7897:Atp13a4
|
UTSW |
16 |
29,396,466 (GRCm38) |
missense |
|
|
|
R7950:Atp13a4
|
UTSW |
16 |
29,449,917 (GRCm38) |
missense |
|
|
|
R8217:Atp13a4
|
UTSW |
16 |
29,403,801 (GRCm38) |
missense |
|
|
|
R8227:Atp13a4
|
UTSW |
16 |
29,403,845 (GRCm38) |
missense |
|
|
|
R8273:Atp13a4
|
UTSW |
16 |
29,471,902 (GRCm38) |
missense |
|
|
|
R8488:Atp13a4
|
UTSW |
16 |
29,417,836 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R8508:Atp13a4
|
UTSW |
16 |
29,454,769 (GRCm38) |
nonsense |
probably null |
|
|
R8773:Atp13a4
|
UTSW |
16 |
29,441,580 (GRCm38) |
missense |
|
|
|
R8921:Atp13a4
|
UTSW |
16 |
29,454,774 (GRCm38) |
missense |
|
|
|
R8940:Atp13a4
|
UTSW |
16 |
29,454,690 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9056:Atp13a4
|
UTSW |
16 |
29,471,888 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9272:Atp13a4
|
UTSW |
16 |
29,449,979 (GRCm38) |
missense |
|
|
|
R9292:Atp13a4
|
UTSW |
16 |
29,422,682 (GRCm38) |
missense |
|
|
|
R9415:Atp13a4
|
UTSW |
16 |
29,409,003 (GRCm38) |
missense |
|
|
|
R9453:Atp13a4
|
UTSW |
16 |
29,420,841 (GRCm38) |
missense |
unknown |
|
|
R9497:Atp13a4
|
UTSW |
16 |
29,469,312 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R9541:Atp13a4
|
UTSW |
16 |
29,422,726 (GRCm38) |
missense |
|
|
|
R9614:Atp13a4
|
UTSW |
16 |
29,441,580 (GRCm38) |
missense |
|
|
|
R9622:Atp13a4
|
UTSW |
16 |
29,420,459 (GRCm38) |
missense |
|
|
|
R9727:Atp13a4
|
UTSW |
16 |
29,409,771 (GRCm38) |
missense |
|
|
|
Z1176:Atp13a4
|
UTSW |
16 |
29,422,587 (GRCm38) |
missense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- CATTGACTTGGGGTTGCCTC -3'
(R):5'- CTGGTCAGGCTGAAACAACC -3'
Sequencing Primer
(F):5'- GGGGTTGCCTCAAAACTTG -3'
(R):5'- AGGCTGAAACAACCTTAGTAATTATC -3'
|
| Posted On |
2014-08-25 |
Incidental Mutation