Mutagenetix > Incidental Mutation

Incidental Mutation 'R4273:Mrgpra3'

322274

Institutional Source

Beutler Lab

Gene Symbol

Mrgpra3

Ensembl Gene

ENSMUSG00000078698

Gene Name

MAS-related GPR, member A3

Synonyms

G protein-coupled receptor, MrgA3

MMRRC Submission

041645-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R4273 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

47588950-47601372 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 47589432 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 249 (W249R)

Ref Sequence

ENSEMBL: ENSMUSP00000135437 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176369]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000176369
AA Change: W249R

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000135437
Gene: ENSMUSG00000078698
AA Change: W249R

Domain	Start	End	E-Value	Type
low complexity region	14	31	N/A	INTRINSIC
Pfam:7TM_GPCR_Srx	48	234	6.3e-7	PFAM
Pfam:7tm_1	57	286	2.7e-9	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.7%
20x: 96.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acer2	T	A	4: 86,874,598		probably null	Het
Adgrf2	T	A	17: 42,710,122	T604S	probably damaging	Het
Akap8l	T	A	17: 32,321,931	K533*	probably null	Het
Appbp2	T	C	11: 85,234,676	Y45C	probably damaging	Het
Arap2	T	C	5: 62,670,979	I950V	possibly damaging	Het
Arhgap31	T	C	16: 38,602,335	E1123G	possibly damaging	Het
Atp2c1	G	T	9: 105,435,140	N493K	probably benign	Het
Bcr	T	C	10: 75,125,111	I458T	probably damaging	Het
Brd4	G	A	17: 32,214,782	T468I	probably benign	Het
Cdh23	T	A	10: 60,311,161	D2774V	possibly damaging	Het
Cfdp1	T	C	8: 111,768,785	Y267C	probably damaging	Het
Chd6	C	A	2: 160,961,291	A2156S	probably benign	Het
Dazap2	C	A	15: 100,618,090	P100T	probably damaging	Het
Disp1	T	A	1: 183,087,644	I1071F	possibly damaging	Het
Dlgap1	T	A	17: 70,766,043	S686T	probably benign	Het
Dst	C	T	1: 34,192,340	R3183C	possibly damaging	Het
Enpp4	T	C	17: 44,101,807	N279D	probably benign	Het
Exoc3l	T	C	8: 105,289,961	*740W	probably null	Het
Exoc5	A	T	14: 49,015,480	C625*	probably null	Het
Fam98b	A	T	2: 117,260,231	N137Y	possibly damaging	Het
Fat4	T	A	3: 38,891,627	D1556E	probably damaging	Het
Fcer2a	C	A	8: 3,682,848	V319L	possibly damaging	Het
Fer1l6	T	C	15: 58,627,522	V1247A	probably benign	Het
Fmo4	A	G	1: 162,805,179	V201A	probably damaging	Het
Fras1	G	A	5: 96,614,904	G755D	probably benign	Het
Gm13078	A	T	4: 143,726,846	K175*	probably null	Het
Grid2	T	C	6: 63,909,045	Y142H	probably damaging	Het
Hspg2	C	T	4: 137,518,940	R1010C	probably damaging	Het
Ibtk	T	C	9: 85,726,731	Q376R	probably damaging	Het
Impdh2	T	C	9: 108,564,956	M414T	probably damaging	Het
Itm2c	A	G	1: 85,907,029	T160A	probably damaging	Het
Kcna2	T	A	3: 107,105,193	D363E	probably benign	Het
Lama2	C	T	10: 27,347,054	C412Y	probably damaging	Het
Lims2	C	G	18: 31,956,337	T151S	probably benign	Het
Mier1	T	C	4: 103,162,431	S423P	possibly damaging	Het
Mtor	A	G	4: 148,550,152	H2410R	probably benign	Het
Mvp	C	T	7: 126,989,703	A631T	probably benign	Het
Nepro	T	C	16: 44,735,829	V450A	possibly damaging	Het
Ngrn	T	C	7: 80,264,521	V140A	probably damaging	Het
Nobox	T	C	6: 43,306,008	E231G	probably benign	Het
Olfr129	A	T	17: 38,055,272	I98N	probably damaging	Het
P3h2	T	A	16: 26,105,221	I155F	probably benign	Het
Pcdha3	C	T	18: 36,948,091	R629C	probably damaging	Het
Riok3	AGAAGCGG	AG	18: 12,135,941		probably benign	Het
Rttn	T	C	18: 89,091,896	I1675T	probably benign	Het
Sall2	C	A	14: 52,313,803	R643L	probably damaging	Het
Slc35f4	G	T	14: 49,304,301	T182N	possibly damaging	Het
Slc52a3	T	A	2: 152,005,740	I256N	possibly damaging	Het
Sox9	T	C	11: 112,785,154	S390P	possibly damaging	Het
Tango2	T	C	16: 18,302,790		probably benign	Het
Tas1r1	T	C	4: 152,032,157	E340G	possibly damaging	Het
Tek	G	A	4: 94,829,970	G524R	probably damaging	Het
Tmem260	A	T	14: 48,505,304	Y532F	probably benign	Het
Tsks	C	A	7: 44,957,929	L559I	probably damaging	Het
Unc79	C	A	12: 103,122,353	L1702I	probably damaging	Het
Vmn1r14	T	A	6: 57,234,148	I237N	probably damaging	Het
Vmn2r17	G	A	5: 109,452,966	C710Y	probably benign	Het
Zfp119b	G	T	17: 55,938,926	T420K	possibly damaging	Het
Zfp202	C	T	9: 40,207,494	R68*	probably null	Het
Zfp229	T	A	17: 21,746,821	S677R	probably benign	Het
Zfp462	C	T	4: 55,008,411	H126Y	probably benign	Het
Zfp52	C	A	17: 21,560,197	Y102*	probably null	Het
Zfp616	T	A	11: 74,083,700	M265K	probably benign	Het
Zfyve9	A	T	4: 108,680,976	I1031N	probably damaging	Het
Zmynd11	T	C	13: 9,697,690	Y203C	probably damaging	Het

Other mutations in Mrgpra3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00162:Mrgpra3	APN	7	47589519	missense	probably benign	0.00
IGL02097:Mrgpra3	APN	7	47589456	missense	possibly damaging	0.94
IGL02101:Mrgpra3	APN	7	47589552	missense	probably damaging	0.99
IGL03071:Mrgpra3	APN	7	47589181	utr 3 prime	probably benign
PIT4445001:Mrgpra3	UTSW	7	47590160	missense	possibly damaging	0.88
R0828:Mrgpra3	UTSW	7	47590136	missense	probably benign	0.12
R1118:Mrgpra3	UTSW	7	47589291	missense	possibly damaging	0.83
R1835:Mrgpra3	UTSW	7	47589946	nonsense	probably null
R2258:Mrgpra3	UTSW	7	47590094	missense	probably benign	0.35
R2393:Mrgpra3	UTSW	7	47589617	missense	possibly damaging	0.92
R3437:Mrgpra3	UTSW	7	47589566	missense	probably damaging	1.00
R3973:Mrgpra3	UTSW	7	47589666	missense	probably benign	0.22
R4495:Mrgpra3	UTSW	7	47590065	missense	probably benign	0.00
R4768:Mrgpra3	UTSW	7	47589728	missense	possibly damaging	0.94
R4822:Mrgpra3	UTSW	7	47589968	missense	possibly damaging	0.69
R4967:Mrgpra3	UTSW	7	47589519	missense	probably benign	0.33
R5207:Mrgpra3	UTSW	7	47590161	missense	probably benign	0.06
R5569:Mrgpra3	UTSW	7	47590011	missense	probably benign	0.00
R5763:Mrgpra3	UTSW	7	47589607	nonsense	probably null
R6102:Mrgpra3	UTSW	7	47590149	missense	possibly damaging	0.92
R6612:Mrgpra3	UTSW	7	47590035	missense	probably benign	0.00
R6718:Mrgpra3	UTSW	7	47589696	missense	probably benign	0.25
R6859:Mrgpra3	UTSW	7	47590033	missense	probably benign	0.04
R7029:Mrgpra3	UTSW	7	47589542	missense	probably benign	0.00
R7034:Mrgpra3	UTSW	7	47590090	missense	possibly damaging	0.49
R7036:Mrgpra3	UTSW	7	47590090	missense	possibly damaging	0.49
R7097:Mrgpra3	UTSW	7	47589641	missense	probably benign
R7475:Mrgpra3	UTSW	7	47589947	missense	probably damaging	1.00
R8289:Mrgpra3	UTSW	7	47589720	missense	possibly damaging	0.76
R8315:Mrgpra3	UTSW	7	47601303	start codon destroyed	probably null	0.93
R9500:Mrgpra3	UTSW	7	47589652	nonsense	probably null
Z1177:Mrgpra3	UTSW	7	47601301	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCATGTTTTCAGGTGTCTCAGG -3'
(R):5'- TACTCTGTGTGTCAGGCATG -3'

Sequencing Primer
(F):5'- TGCAGGGCTTTCTGGAGAACC -3'
(R):5'- GGGACATCTTTATCGGAGCATACC -3'

Posted On

2015-06-20