Mutagenetix > Incidental Mutation

Incidental Mutation 'R7781:Mga'

599213

Institutional Source

Beutler Lab

Gene Symbol

Mga

Ensembl Gene

ENSMUSG00000033943

Gene Name

MAX gene associated

Synonyms

Mga, C130042M01Rik, Mad5, D030062C11Rik

MMRRC Submission

045837-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.953) question?

Stock #

R7781 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

119727709-119800062 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 119747838 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 663 (V663D)

Ref Sequence

ENSEMBL: ENSMUSP00000119044 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046717] [ENSMUST00000079934] [ENSMUST00000110773] [ENSMUST00000110774] [ENSMUST00000156510]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000046717
AA Change: V663D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000043795
Gene: ENSMUSG00000033943
AA Change: V663D

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	6e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1248	1269	N/A	INTRINSIC
low complexity region	1301	1315	N/A	INTRINSIC
low complexity region	1564	1581	N/A	INTRINSIC
low complexity region	1634	1649	N/A	INTRINSIC
low complexity region	1681	1716	N/A	INTRINSIC
low complexity region	1796	1818	N/A	INTRINSIC
low complexity region	1833	1850	N/A	INTRINSIC
low complexity region	1977	1992	N/A	INTRINSIC
low complexity region	2183	2197	N/A	INTRINSIC
low complexity region	2241	2259	N/A	INTRINSIC
HLH	2368	2419	8.27e-7	SMART
low complexity region	2748	2769	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000079934
AA Change: V663D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078853
Gene: ENSMUSG00000033943
AA Change: V663D

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	5e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1247	1268	N/A	INTRINSIC
low complexity region	1300	1314	N/A	INTRINSIC
low complexity region	1626	1648	N/A	INTRINSIC
low complexity region	1663	1680	N/A	INTRINSIC
low complexity region	1807	1822	N/A	INTRINSIC
low complexity region	2013	2027	N/A	INTRINSIC
low complexity region	2071	2089	N/A	INTRINSIC
HLH	2198	2249	8.27e-7	SMART
low complexity region	2578	2599	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110773
AA Change: V663D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000106400
Gene: ENSMUSG00000033943
AA Change: V663D

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	5e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	890	899	N/A	INTRINSIC
low complexity region	938	952	N/A	INTRINSIC
low complexity region	1033	1059	N/A	INTRINSIC
low complexity region	1169	1190	N/A	INTRINSIC
low complexity region	1222	1236	N/A	INTRINSIC
low complexity region	1485	1502	N/A	INTRINSIC
low complexity region	1555	1570	N/A	INTRINSIC
low complexity region	1602	1637	N/A	INTRINSIC
low complexity region	1717	1739	N/A	INTRINSIC
low complexity region	1754	1771	N/A	INTRINSIC
low complexity region	1898	1913	N/A	INTRINSIC
low complexity region	2104	2118	N/A	INTRINSIC
low complexity region	2162	2180	N/A	INTRINSIC
HLH	2289	2340	8.27e-7	SMART
low complexity region	2669	2690	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110774
AA Change: V663D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000106401
Gene: ENSMUSG00000033943
AA Change: V663D

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	7e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
Pfam:DUF4801	1037	1085	1e-19	PFAM
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1248	1269	N/A	INTRINSIC
low complexity region	1301	1315	N/A	INTRINSIC
low complexity region	1564	1581	N/A	INTRINSIC
low complexity region	1634	1649	N/A	INTRINSIC
low complexity region	1681	1716	N/A	INTRINSIC
low complexity region	1835	1857	N/A	INTRINSIC
low complexity region	1872	1889	N/A	INTRINSIC
low complexity region	2016	2031	N/A	INTRINSIC
low complexity region	2222	2236	N/A	INTRINSIC
low complexity region	2280	2298	N/A	INTRINSIC
HLH	2407	2458	8.27e-7	SMART
low complexity region	2787	2808	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000156510
AA Change: V663D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000119044
Gene: ENSMUSG00000033943
AA Change: V663D

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	4e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1247	1268	N/A	INTRINSIC
low complexity region	1300	1314	N/A	INTRINSIC
low complexity region	1626	1648	N/A	INTRINSIC
low complexity region	1663	1680	N/A	INTRINSIC
low complexity region	1807	1822	N/A	INTRINSIC
low complexity region	2013	2027	N/A	INTRINSIC
low complexity region	2071	2089	N/A	INTRINSIC
HLH	2198	2249	8.27e-7	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Embryos homozygous for a gene trap allele die shortly after implantation due to defective development of the inner cell mass (ICM) and the epiblast. ICM derivatives fail to develop past E4.5 and show increased apoptosis but no change in cell proliferation. [provided by MGI curators]

Allele List at MGI

All alleles(135) : Gene trapped(135)

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700064H15Rik	G	A	3: 19,682,706 (GRCm39)	P23S	unknown	Het
Abcc6	C	T	7: 45,655,030 (GRCm39)	R487Q	probably damaging	Het
Abl1	A	T	2: 31,680,709 (GRCm39)	T334S	probably damaging	Het
Adam6b	T	C	12: 113,454,962 (GRCm39)	F593S	probably damaging	Het
Alx1	A	T	10: 102,845,053 (GRCm39)	M326K	probably damaging	Het
Ankhd1	A	T	18: 36,758,258 (GRCm39)	D984V	probably damaging	Het
Asb15	A	G	6: 24,562,644 (GRCm39)	N202S	probably benign	Het
Ate1	A	T	7: 130,121,157 (GRCm39)	V12E	probably damaging	Het
Atl2	A	T	17: 80,167,260 (GRCm39)	Y254N	probably damaging	Het
Atp13a5	A	G	16: 29,116,226 (GRCm39)	M630T	probably benign	Het
Atp5po	A	T	16: 91,723,417 (GRCm39)	I124N	possibly damaging	Het
BC005537	C	T	13: 24,987,382 (GRCm39)	R7W	possibly damaging	Het
Bicdl1	T	C	5: 115,799,546 (GRCm39)	E181G	probably damaging	Het
Bnipl	C	A	3: 95,151,486 (GRCm39)	W272L	probably damaging	Het
Catsperd	A	T	17: 56,971,072 (GRCm39)	H712L	probably benign	Het
Cilp2	T	C	8: 70,334,997 (GRCm39)	D667G	possibly damaging	Het
Cntn4	A	C	6: 106,500,575 (GRCm39)	K351Q	probably damaging	Het
Coq7	A	T	7: 118,125,111 (GRCm39)	I171N	probably damaging	Het
Csf2rb	T	A	15: 78,228,771 (GRCm39)	F371I	probably benign	Het
Cyp3a13	C	T	5: 137,897,136 (GRCm39)	V393M	possibly damaging	Het
Dennd2a	A	G	6: 39,470,000 (GRCm39)	V564A	probably damaging	Het
Ear14	T	C	14: 51,441,468 (GRCm39)	L108P	probably damaging	Het
Egflam	C	A	15: 7,283,227 (GRCm39)	V277F	probably null	Het
Epb42	T	G	2: 120,864,916 (GRCm39)	K58N	probably benign	Het
Faap100	T	C	11: 120,265,089 (GRCm39)	M596V	probably benign	Het
Fbxw15	T	A	9: 109,386,330 (GRCm39)	T270S	possibly damaging	Het
Gabpb1	C	T	2: 126,481,120 (GRCm39)	C342Y	possibly damaging	Het
Gls	A	T	1: 52,251,492 (GRCm39)	C288*	probably null	Het
Grm8	G	T	6: 27,285,786 (GRCm39)	D875E	probably benign	Het
Hdac4	C	A	1: 91,903,387 (GRCm39)	R514L	probably benign	Het
Hps4	T	A	5: 112,518,388 (GRCm39)	N460K	probably benign	Het
Inpp5d	T	C	1: 87,627,394 (GRCm39)	F565S	probably damaging	Het
Kcnh5	C	A	12: 75,023,455 (GRCm39)	V538F	probably damaging	Het
Kcp	T	C	6: 29,497,764 (GRCm39)	N499S	probably damaging	Het
Klk15	T	C	7: 43,588,980 (GRCm39)	L244S	probably benign	Het
Lamp3	A	T	16: 19,518,440 (GRCm39)	S266T	possibly damaging	Het
Lemd3	A	G	10: 120,761,678 (GRCm39)	F863L	probably damaging	Het
Naa15	A	G	3: 51,378,904 (GRCm39)		probably null	Het
Ndrg3	C	A	2: 156,770,733 (GRCm39)	G352*	probably null	Het
Nr2f6	G	T	8: 71,828,595 (GRCm39)	N233K	possibly damaging	Het
Or4a79	A	G	2: 89,551,879 (GRCm39)	F192S	probably benign	Het
Plxnb2	T	A	15: 89,041,225 (GRCm39)	M1774L	possibly damaging	Het
Repin1	G	T	6: 48,574,279 (GRCm39)	E403*	probably null	Het
Rpl23a	G	A	11: 78,073,654 (GRCm39)	R62W	probably benign	Het
Rubcnl	A	G	14: 75,269,530 (GRCm39)	R63G	probably damaging	Het
Ryr1	T	C	7: 28,767,055 (GRCm39)	D2976G	probably damaging	Het
Siglec1	A	G	2: 130,923,258 (GRCm39)	Y496H	probably damaging	Het
Sipa1l2	A	G	8: 126,218,566 (GRCm39)	V257A	possibly damaging	Het
Slc24a4	T	C	12: 102,201,112 (GRCm39)		probably null	Het
Slc4a1ap	T	C	5: 31,684,822 (GRCm39)	S153P	probably damaging	Het
Slc4a4	A	T	5: 89,376,791 (GRCm39)	E1006V	possibly damaging	Het
Svep1	T	G	4: 58,069,251 (GRCm39)	E2845A	possibly damaging	Het
Tlr1	T	C	5: 65,084,079 (GRCm39)	N166S	possibly damaging	Het
Tmem231	C	T	8: 112,644,922 (GRCm39)		probably null	Het
Tsc2	C	A	17: 24,827,089 (GRCm39)	L873F	possibly damaging	Het
Unc13b	T	G	4: 43,259,546 (GRCm39)	S1403A	possibly damaging	Het
Vmn1r7	A	G	6: 57,001,553 (GRCm39)	F236L	probably benign	Het
Vmn2r111	T	C	17: 22,789,714 (GRCm39)	S431G	probably benign	Het
Wdr7	A	T	18: 63,910,860 (GRCm39)	K751*	probably null	Het
Wdr90	C	T	17: 26,065,300 (GRCm39)	R1652Q	probably damaging	Het
Zcchc2	T	C	1: 105,931,895 (GRCm39)	Y366H	probably damaging	Het
Zfp1005	A	T	2: 150,109,577 (GRCm39)	H89L	possibly damaging	Het
Zfp607a	C	T	7: 27,565,000 (GRCm39)	R56C	possibly damaging	Het

Other mutations in Mga

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Mga	APN	2	119,750,295 (GRCm39)	missense	possibly damaging	0.65
IGL00719:Mga	APN	2	119,777,934 (GRCm39)	nonsense	probably null
IGL01619:Mga	APN	2	119,762,309 (GRCm39)	missense	possibly damaging	0.46
IGL01721:Mga	APN	2	119,765,720 (GRCm39)	missense	probably damaging	1.00
IGL01759:Mga	APN	2	119,781,676 (GRCm39)	missense	possibly damaging	0.92
IGL01785:Mga	APN	2	119,733,393 (GRCm39)	missense	probably damaging	1.00
IGL01786:Mga	APN	2	119,733,393 (GRCm39)	missense	probably damaging	1.00
IGL01950:Mga	APN	2	119,772,135 (GRCm39)	missense	possibly damaging	0.60
IGL01960:Mga	APN	2	119,769,138 (GRCm39)	missense	probably damaging	1.00
IGL02086:Mga	APN	2	119,754,517 (GRCm39)	missense	probably damaging	0.99
IGL02364:Mga	APN	2	119,794,535 (GRCm39)	missense	possibly damaging	0.66
IGL02602:Mga	APN	2	119,762,365 (GRCm39)	missense	possibly damaging	0.66
IGL02751:Mga	APN	2	119,778,251 (GRCm39)	missense	possibly damaging	0.82
IGL02794:Mga	APN	2	119,776,770 (GRCm39)	missense	possibly damaging	0.84
IGL03247:Mga	APN	2	119,765,994 (GRCm39)	missense	possibly damaging	0.81
IGL03303:Mga	APN	2	119,733,933 (GRCm39)	missense	probably damaging	1.00
PIT4515001:Mga	UTSW	2	119,746,985 (GRCm39)	missense	probably damaging	1.00
R0060:Mga	UTSW	2	119,791,442 (GRCm39)	critical splice donor site	probably null
R0060:Mga	UTSW	2	119,791,442 (GRCm39)	critical splice donor site	probably null
R0417:Mga	UTSW	2	119,733,271 (GRCm39)	missense	probably damaging	0.99
R0449:Mga	UTSW	2	119,771,862 (GRCm39)	missense	probably damaging	1.00
R0457:Mga	UTSW	2	119,746,969 (GRCm39)	missense	probably damaging	0.98
R0538:Mga	UTSW	2	119,750,187 (GRCm39)	critical splice donor site	probably null
R0568:Mga	UTSW	2	119,765,903 (GRCm39)	missense	probably damaging	1.00
R0614:Mga	UTSW	2	119,794,947 (GRCm39)	missense	probably damaging	1.00
R0671:Mga	UTSW	2	119,750,391 (GRCm39)	splice site	probably null
R0811:Mga	UTSW	2	119,778,442 (GRCm39)	missense	probably damaging	0.99
R0812:Mga	UTSW	2	119,778,442 (GRCm39)	missense	probably damaging	0.99
R0948:Mga	UTSW	2	119,772,140 (GRCm39)	missense	possibly damaging	0.77
R1177:Mga	UTSW	2	119,756,927 (GRCm39)	missense	probably damaging	1.00
R1445:Mga	UTSW	2	119,733,179 (GRCm39)	missense	probably damaging	1.00
R1476:Mga	UTSW	2	119,772,156 (GRCm39)	missense	probably damaging	0.96
R1527:Mga	UTSW	2	119,747,078 (GRCm39)	missense	probably damaging	1.00
R1583:Mga	UTSW	2	119,794,441 (GRCm39)	missense	possibly damaging	0.66
R1592:Mga	UTSW	2	119,795,147 (GRCm39)	missense	possibly damaging	0.93
R1627:Mga	UTSW	2	119,795,043 (GRCm39)	missense	probably damaging	1.00
R1658:Mga	UTSW	2	119,772,170 (GRCm39)	missense	possibly damaging	0.63
R1677:Mga	UTSW	2	119,791,333 (GRCm39)	missense	possibly damaging	0.92
R1887:Mga	UTSW	2	119,754,098 (GRCm39)	missense	probably damaging	1.00
R1908:Mga	UTSW	2	119,757,075 (GRCm39)	missense	possibly damaging	0.66
R1909:Mga	UTSW	2	119,757,075 (GRCm39)	missense	possibly damaging	0.66
R2061:Mga	UTSW	2	119,795,461 (GRCm39)	unclassified	probably benign
R2145:Mga	UTSW	2	119,794,638 (GRCm39)	missense	possibly damaging	0.85
R2159:Mga	UTSW	2	119,750,124 (GRCm39)	missense	probably damaging	0.96
R2179:Mga	UTSW	2	119,790,923 (GRCm39)	missense	probably damaging	0.99
R2281:Mga	UTSW	2	119,734,204 (GRCm39)	missense	probably benign
R2423:Mga	UTSW	2	119,795,274 (GRCm39)	missense	probably damaging	1.00
R3620:Mga	UTSW	2	119,747,149 (GRCm39)	missense	probably damaging	1.00
R3622:Mga	UTSW	2	119,772,245 (GRCm39)	missense	probably damaging	1.00
R3624:Mga	UTSW	2	119,772,245 (GRCm39)	missense	probably damaging	1.00
R3802:Mga	UTSW	2	119,777,820 (GRCm39)	missense	probably damaging	0.96
R4011:Mga	UTSW	2	119,762,261 (GRCm39)	missense	probably damaging	1.00
R4065:Mga	UTSW	2	119,777,483 (GRCm39)	missense	probably damaging	1.00
R4520:Mga	UTSW	2	119,778,579 (GRCm39)	missense	possibly damaging	0.85
R4649:Mga	UTSW	2	119,771,974 (GRCm39)	missense	possibly damaging	0.81
R4660:Mga	UTSW	2	119,769,104 (GRCm39)	intron	probably benign
R4757:Mga	UTSW	2	119,734,120 (GRCm39)	missense	possibly damaging	0.82
R4771:Mga	UTSW	2	119,794,775 (GRCm39)	missense	probably damaging	1.00
R4784:Mga	UTSW	2	119,733,538 (GRCm39)	missense	probably damaging	1.00
R4866:Mga	UTSW	2	119,794,535 (GRCm39)	missense	possibly damaging	0.66
R4900:Mga	UTSW	2	119,794,535 (GRCm39)	missense	possibly damaging	0.66
R4952:Mga	UTSW	2	119,733,782 (GRCm39)	missense	probably damaging	1.00
R4995:Mga	UTSW	2	119,763,063 (GRCm39)	nonsense	probably null
R5020:Mga	UTSW	2	119,781,654 (GRCm39)	nonsense	probably null
R5082:Mga	UTSW	2	119,733,825 (GRCm39)	missense	probably damaging	0.98
R5208:Mga	UTSW	2	119,778,462 (GRCm39)	missense	possibly damaging	0.83
R5454:Mga	UTSW	2	119,733,810 (GRCm39)	missense	probably damaging	0.99
R5466:Mga	UTSW	2	119,733,178 (GRCm39)	missense	probably damaging	1.00
R5484:Mga	UTSW	2	119,747,107 (GRCm39)	missense	possibly damaging	0.58
R5669:Mga	UTSW	2	119,733,907 (GRCm39)	missense	probably damaging	1.00
R5819:Mga	UTSW	2	119,771,744 (GRCm39)	missense	possibly damaging	0.61
R5916:Mga	UTSW	2	119,794,793 (GRCm39)	missense	probably benign	0.27
R5942:Mga	UTSW	2	119,777,440 (GRCm39)	missense	probably benign	0.41
R6305:Mga	UTSW	2	119,778,179 (GRCm39)	missense	probably benign	0.00
R6434:Mga	UTSW	2	119,754,419 (GRCm39)	missense	probably damaging	0.99
R6467:Mga	UTSW	2	119,776,776 (GRCm39)	missense	probably damaging	1.00
R6488:Mga	UTSW	2	119,791,388 (GRCm39)	missense	probably damaging	1.00
R6630:Mga	UTSW	2	119,754,140 (GRCm39)	missense	probably damaging	0.99
R6790:Mga	UTSW	2	119,754,235 (GRCm39)	missense	probably damaging	0.99
R7029:Mga	UTSW	2	119,754,031 (GRCm39)	missense	probably damaging	1.00
R7039:Mga	UTSW	2	119,763,159 (GRCm39)	missense	probably benign	0.28
R7088:Mga	UTSW	2	119,792,417 (GRCm39)	missense	probably damaging	1.00
R7195:Mga	UTSW	2	119,747,809 (GRCm39)	missense	probably damaging	1.00
R7273:Mga	UTSW	2	119,765,695 (GRCm39)	missense	probably damaging	1.00
R7286:Mga	UTSW	2	119,795,269 (GRCm39)	missense	possibly damaging	0.93
R7346:Mga	UTSW	2	119,766,008 (GRCm39)	missense	possibly damaging	0.56
R7383:Mga	UTSW	2	119,790,821 (GRCm39)	missense	probably damaging	0.99
R7469:Mga	UTSW	2	119,733,527 (GRCm39)	missense	probably damaging	1.00
R7484:Mga	UTSW	2	119,776,710 (GRCm39)	missense	probably damaging	0.99
R7537:Mga	UTSW	2	119,766,032 (GRCm39)	missense	probably damaging	0.97
R7921:Mga	UTSW	2	119,750,159 (GRCm39)	missense	probably damaging	1.00
R8165:Mga	UTSW	2	119,777,719 (GRCm39)	missense	probably benign	0.12
R8226:Mga	UTSW	2	119,790,866 (GRCm39)	missense	probably benign	0.33
R8305:Mga	UTSW	2	119,776,800 (GRCm39)	missense	possibly damaging	0.77
R8309:Mga	UTSW	2	119,791,411 (GRCm39)	missense	probably damaging	1.00
R8363:Mga	UTSW	2	119,794,407 (GRCm39)	missense	probably benign	0.43
R8388:Mga	UTSW	2	119,794,562 (GRCm39)	missense	probably benign	0.00
R8524:Mga	UTSW	2	119,771,997 (GRCm39)	missense	probably damaging	0.97
R8693:Mga	UTSW	2	119,794,407 (GRCm39)	missense	possibly damaging	0.65
R8837:Mga	UTSW	2	119,769,272 (GRCm39)	splice site	probably benign
R8916:Mga	UTSW	2	119,788,819 (GRCm39)	missense	possibly damaging	0.92
R8936:Mga	UTSW	2	119,794,709 (GRCm39)	missense	probably damaging	1.00
R9028:Mga	UTSW	2	119,778,070 (GRCm39)	missense	probably benign
R9145:Mga	UTSW	2	119,794,493 (GRCm39)	missense	probably benign
R9155:Mga	UTSW	2	119,757,013 (GRCm39)	missense	probably damaging	1.00
R9308:Mga	UTSW	2	119,754,369 (GRCm39)	missense	possibly damaging	0.91
R9342:Mga	UTSW	2	119,778,656 (GRCm39)	missense	probably benign
R9347:Mga	UTSW	2	119,733,518 (GRCm39)	missense	probably damaging	1.00
R9390:Mga	UTSW	2	119,794,332 (GRCm39)	missense	probably damaging	0.99
R9408:Mga	UTSW	2	119,765,999 (GRCm39)	missense	possibly damaging	0.92
R9488:Mga	UTSW	2	119,795,304 (GRCm39)	missense	possibly damaging	0.90
R9495:Mga	UTSW	2	119,781,676 (GRCm39)	missense	possibly damaging	0.92
R9521:Mga	UTSW	2	119,794,979 (GRCm39)	missense	probably damaging	0.99
R9780:Mga	UTSW	2	119,747,253 (GRCm39)	missense	probably benign	0.26

Predicted Primers

PCR Primer
(F):5'- GGCAATACCATCAAAGACTGTGAC -3'
(R):5'- GCAAGTATCTTAAGAGATGATTACTGT -3'

Sequencing Primer
(F):5'- TACCATCAAAGACTGTGACTGCTAG -3'
(R):5'- CCAAATGTTTAACACTCACTC -3'

Posted On

2019-11-26