Mutagenetix > Incidental Mutation

Incidental Mutation 'R1697:Wls'

192312

Institutional Source

Beutler Lab

Gene Symbol

Wls

Ensembl Gene

ENSMUSG00000028173

Gene Name

wntless WNT ligand secretion mediator

Synonyms

5031439A09Rik, Gpr177

MMRRC Submission

039730-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1697 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

159839672-159938664 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 159897358 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 136 (V136A)

Ref Sequence

ENSEMBL: ENSMUSP00000143475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068952] [ENSMUST00000198878] [ENSMUST00000200191]

AlphaFold

Q6DID7

Predicted Effect

probably benign
Transcript: ENSMUST00000068952
AA Change: V136A

PolyPhen 2 Score 0.117 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000067898
Gene: ENSMUSG00000028173
AA Change: V136A

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	36	47	N/A	INTRINSIC
Pfam:MIG-14_Wnt-bd	178	496	3.7e-97	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197328

Predicted Effect

probably benign
Transcript: ENSMUST00000198878
AA Change: V136A

PolyPhen 2 Score 0.117 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000143475
Gene: ENSMUSG00000028173
AA Change: V136A

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	36	47	N/A	INTRINSIC
Pfam:MIG-14_Wnt-bd	177	497	2.8e-94	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000200191

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200571

Meta Mutation Damage Score

0.1228

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.3%

Validation Efficiency

99% (67/68)

MGI Phenotype

PHENOTYPE: Mice homozygous for null alleles exhibit impaired body axis and triploblastic development dying prior to E10.5. Mice homozygous for a floxed allele activated in keratinocytes exhibit a psoriasiform dermatitis-like phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930486L24Rik	A	T	13: 60,845,114	D250E	probably damaging	Het
4930571K23Rik	A	G	7: 125,369,029		noncoding transcript	Het
9530053A07Rik	T	A	7: 28,154,347	C1579S	probably damaging	Het
Acsl3	T	C	1: 78,705,397		probably benign	Het
Acsl6	C	A	11: 54,329,966	T244K	probably damaging	Het
Adam26b	T	A	8: 43,520,963	N334I	probably damaging	Het
Adgrl4	C	T	3: 151,517,611	T608M	probably damaging	Het
Aldh2	A	G	5: 121,578,341		probably null	Het
Alms1	A	G	6: 85,622,454	T1890A	possibly damaging	Het
C87977	A	C	4: 144,208,592	I193S	probably damaging	Het
Capn7	C	T	14: 31,360,160	T441M	probably damaging	Het
Cd9	A	T	6: 125,464,404	C85S	probably damaging	Het
Chrm3	T	C	13: 9,878,758	T81A	probably damaging	Het
Ctif	A	G	18: 75,624,305		probably benign	Het
Dcc	T	A	18: 71,370,737	D950V	probably damaging	Het
Eif4g1	T	C	16: 20,679,780	V422A	probably damaging	Het
Enthd1	A	G	15: 80,452,923	S437P	probably damaging	Het
Fads1	A	G	19: 10,194,100		probably benign	Het
Fat3	T	A	9: 15,944,880	I3869L	probably benign	Het
Fbxw5	T	A	2: 25,502,461	V85E	possibly damaging	Het
Fem1b	T	C	9: 62,797,174	D268G	possibly damaging	Het
Focad	T	C	4: 88,408,988	L1772P	probably damaging	Het
Gm9573	A	C	17: 35,620,648		probably benign	Het
Gm9833	G	A	3: 10,089,553	V461I	possibly damaging	Het
Gtf3a	C	A	5: 146,951,913	Q145K	possibly damaging	Het
Hacl1	T	C	14: 31,621,000		probably null	Het
Herc2	T	A	7: 56,153,905	F2229L	probably benign	Het
Hs3st4	A	T	7: 124,396,857	I249L	probably benign	Het
Iqsec1	A	T	6: 90,809,770	Y7*	probably null	Het
Klk1b1	T	A	7: 43,970,326	M103K	probably benign	Het
Krt5	A	G	15: 101,710,585	V287A	probably benign	Het
Lgals12	T	A	19: 7,604,165	Q59L	possibly damaging	Het
Loxl4	A	G	19: 42,604,940	V264A	possibly damaging	Het
Lrmp	A	G	6: 145,137,615		probably benign	Het
Lrp1b	T	C	2: 40,822,683	D3099G	probably damaging	Het
Mical3	G	A	6: 121,007,408	T169I	possibly damaging	Het
Myh7b	A	C	2: 155,620,134	S317R	probably damaging	Het
Nrbp1	T	A	5: 31,245,813	I210N	probably damaging	Het
Nsd1	A	T	13: 55,214,059		probably null	Het
Nupl1	A	T	14: 60,244,670		probably benign	Het
Olfr152	T	A	2: 87,782,585	I15N	possibly damaging	Het
Olfr190	A	G	16: 59,074,907	Y58H	probably damaging	Het
Olfr331	A	T	11: 58,501,676	S293R	probably damaging	Het
Olfr346	C	T	2: 36,688,247	L82F	probably damaging	Het
Olfr769	T	C	10: 129,111,868	T186A	probably benign	Het
Pcnx2	A	G	8: 125,850,348	Y982H	probably damaging	Het
Pias3	T	C	3: 96,702,225	L312P	probably damaging	Het
Plekhm1	G	A	11: 103,376,884	P754S	probably damaging	Het
Ppp2r5c	T	A	12: 110,545,623	L145*	probably null	Het
Ppp2r5c	T	A	12: 110,561,472		probably benign	Het
Proser3	T	C	7: 30,540,021	M553V	probably benign	Het
Shf	G	A	2: 122,368,682	P51S	probably damaging	Het
Smurf2	A	T	11: 106,824,688	D664E	possibly damaging	Het
Spag9	G	A	11: 93,996,565	A99T	probably benign	Het
Stim1	T	G	7: 102,354,506	C49G	probably damaging	Het
Stk32c	T	C	7: 139,121,824	I238V	probably benign	Het
Tenm2	C	T	11: 36,063,177	G1236R	possibly damaging	Het
Tfb2m	T	A	1: 179,544,899	E133V	probably null	Het
Tmem209	A	T	6: 30,497,868	C143S	probably benign	Het
Tnr	T	G	1: 159,852,030	N191K	probably benign	Het
Vars	C	T	17: 34,998,222	A419T	probably benign	Het
Vmn2r111	T	C	17: 22,548,060	S819G	probably benign	Het
Ybx2	C	T	11: 69,940,061	S217L	probably benign	Het
Zfp82	T	C	7: 30,057,354	D37G	probably benign	Het

Other mutations in Wls

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01924:Wls	APN	3	159901443	nonsense	probably null
IGL02065:Wls	APN	3	159911356	missense	probably damaging	0.99
IGL02073:Wls	APN	3	159907253	critical splice donor site	probably null
IGL02133:Wls	APN	3	159897370	missense	probably damaging	1.00
R0374:Wls	UTSW	3	159897437	nonsense	probably null
R0561:Wls	UTSW	3	159873068	missense	probably benign	0.32
R1791:Wls	UTSW	3	159911813	missense	probably benign	0.17
R2444:Wls	UTSW	3	159907230	missense	probably damaging	1.00
R3161:Wls	UTSW	3	159897436	missense	probably damaging	1.00
R4285:Wls	UTSW	3	159934266	missense	probably benign
R4468:Wls	UTSW	3	159872927	missense	probably damaging	0.96
R4472:Wls	UTSW	3	159897383	missense	probably benign	0.01
R4782:Wls	UTSW	3	159897445	missense	probably benign	0.03
R4799:Wls	UTSW	3	159897445	missense	probably benign	0.03
R4809:Wls	UTSW	3	159897445	missense	probably benign	0.03
R5006:Wls	UTSW	3	159911791	missense	possibly damaging	0.68
R5212:Wls	UTSW	3	159873008	missense	probably benign	0.15
R5434:Wls	UTSW	3	159934340	missense	probably damaging	0.97
R5694:Wls	UTSW	3	159839987	missense	probably benign	0.01
R6315:Wls	UTSW	3	159934371	critical splice donor site	probably null
R7069:Wls	UTSW	3	159934329	missense	probably damaging	1.00
R7243:Wls	UTSW	3	159909765	missense	possibly damaging	0.49
R7529:Wls	UTSW	3	159873007	missense	probably benign	0.43
R7697:Wls	UTSW	3	159911318	missense	probably benign	0.21
R7842:Wls	UTSW	3	159873179	missense	probably benign	0.09
R8136:Wls	UTSW	3	159873124	missense	probably damaging	1.00
R8536:Wls	UTSW	3	159873111	missense	probably damaging	0.96
R8816:Wls	UTSW	3	159934292	missense	possibly damaging	0.89
R9074:Wls	UTSW	3	159909766	missense	possibly damaging	0.86

Predicted Primers

PCR Primer
(F):5'- TGAGCATCTGAGCTGCTTGAGTCC -3'
(R):5'- GGTAGTACTCCATGCCAAGAATCCAAC -3'

Sequencing Primer
(F):5'- TCTTGACTGCAAAGGGTAGGC -3'
(R):5'- CACTGTCAACTCAGCAATAAAGG -3'

Posted On

2014-05-14