Mutagenetix > Incidental Mutation

Incidental Mutation 'R5469:Vmn2r77'

433409

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r77

Ensembl Gene

ENSMUSG00000090949

Gene Name

vomeronasal 2, receptor 77

Synonyms

EG546983

MMRRC Submission

043030-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R5469 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86444349-86461240 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 86451271 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 386 (M386L)

Ref Sequence

ENSEMBL: ENSMUSP00000129540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164996]

AlphaFold

L7N2B7

Predicted Effect

probably benign
Transcript: ENSMUST00000164996
AA Change: M386L

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000129540
Gene: ENSMUSG00000090949
AA Change: M386L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	467	1.4e-30	PFAM
Pfam:NCD3G	510	562	1e-20	PFAM
Pfam:7tm_3	594	830	2.6e-52	PFAM

Coding Region Coverage

1x: 98.2%
3x: 97.3%
10x: 95.1%
20x: 90.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Allc	A	T	12: 28,605,305 (GRCm39)	N331K	probably benign	Het
Anapc1	G	T	2: 128,517,621 (GRCm39)	S341*	probably null	Het
Cacna1e	T	C	1: 154,319,683 (GRCm39)	E1339G	probably damaging	Het
Cacna2d1	T	A	5: 16,557,676 (GRCm39)	I702N	probably damaging	Het
Casp2	C	A	6: 42,246,268 (GRCm39)	H209N	probably benign	Het
Casr	A	G	16: 36,330,392 (GRCm39)	V314A	probably benign	Het
Ccne2	A	T	4: 11,201,353 (GRCm39)	R294*	probably null	Het
Cd180	T	A	13: 102,841,342 (GRCm39)	H129Q	probably benign	Het
Chst10	A	T	1: 38,904,608 (GRCm39)	Y362N	probably damaging	Het
Ctnna1	T	A	18: 35,372,573 (GRCm39)	D509E	probably benign	Het
Ctsh	G	T	9: 89,942,564 (GRCm39)		probably null	Het
Dhx29	C	T	13: 113,081,073 (GRCm39)	A369V	possibly damaging	Het
Enox1	A	G	14: 77,830,414 (GRCm39)	T340A	probably benign	Het
Fam135b	T	A	15: 71,317,892 (GRCm39)	T1357S	probably benign	Het
Flt3	A	T	5: 147,291,893 (GRCm39)	S544T	possibly damaging	Het
Gm12689	T	C	4: 99,184,402 (GRCm39)	I85T	unknown	Het
Gsap	A	T	5: 21,495,542 (GRCm39)	Y831F	possibly damaging	Het
Hnrnpr	C	T	4: 136,046,745 (GRCm39)	T142M	probably damaging	Het
Jak3	A	G	8: 72,131,417 (GRCm39)	D94G	probably benign	Het
Ktn1	A	T	14: 47,928,377 (GRCm39)	E579D	probably damaging	Het
Lama2	G	A	10: 26,917,185 (GRCm39)	P2247S	possibly damaging	Het
Lrba	C	T	3: 86,449,948 (GRCm39)	S2089F	probably damaging	Het
Map1b	C	T	13: 99,565,846 (GRCm39)	V2292M	unknown	Het
Mphosph10	A	T	7: 64,039,193 (GRCm39)		probably null	Het
Pappa	C	T	4: 65,123,389 (GRCm39)	T908M	probably benign	Het
Pdcd10	T	A	3: 75,428,364 (GRCm39)	K150*	probably null	Het
Piezo2	A	T	18: 63,160,935 (GRCm39)	I2275N	probably damaging	Het
Pmvk	T	C	3: 89,374,989 (GRCm39)		probably null	Het
Pold2	G	A	11: 5,823,048 (GRCm39)	P376S	probably damaging	Het
Prtg	A	T	9: 72,799,247 (GRCm39)	Q759L	probably damaging	Het
Rad51ap1	C	T	6: 126,905,190 (GRCm39)	S107N	probably damaging	Het
Rfk	T	A	19: 17,372,566 (GRCm39)	N29K	probably damaging	Het
Ror2	T	C	13: 53,271,375 (GRCm39)	M315V	probably benign	Het
Rrn3	A	G	16: 13,630,964 (GRCm39)	E600G	probably benign	Het
Ryk	A	G	9: 102,784,153 (GRCm39)	Y593C	possibly damaging	Het
Slc30a3	T	A	5: 31,246,004 (GRCm39)	D193V	probably damaging	Het
Slc35e2	C	T	4: 155,694,483 (GRCm39)	P10L	probably benign	Het
Srbd1	C	A	17: 86,427,370 (GRCm39)	C421F	possibly damaging	Het
Sstr5	A	C	17: 25,711,043 (GRCm39)	V62G	probably damaging	Het
Tfip11	G	A	5: 112,482,191 (GRCm39)	W483*	probably null	Het
Tlk1	T	C	2: 70,552,012 (GRCm39)	H553R	probably benign	Het
Tnc	T	C	4: 63,932,162 (GRCm39)		probably null	Het
Trav12-1	A	G	14: 53,775,930 (GRCm39)	T27A	probably damaging	Het
Usp9y	G	A	Y: 1,364,714 (GRCm39)	T1033I	probably benign	Het
V1ra8	C	T	6: 90,180,186 (GRCm39)	H130Y	probably benign	Het

Other mutations in Vmn2r77

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00954:Vmn2r77	APN	7	86,449,975 (GRCm39)	missense	probably benign	0.06
IGL01105:Vmn2r77	APN	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
IGL01367:Vmn2r77	APN	7	86,461,124 (GRCm39)	missense	probably damaging	0.98
IGL01634:Vmn2r77	APN	7	86,460,857 (GRCm39)	missense	probably benign
IGL01805:Vmn2r77	APN	7	86,460,395 (GRCm39)	missense	probably benign	0.18
IGL01868:Vmn2r77	APN	7	86,452,224 (GRCm39)	missense	probably benign	0.00
IGL01980:Vmn2r77	APN	7	86,450,678 (GRCm39)	missense	probably benign	0.14
IGL02055:Vmn2r77	APN	7	86,450,763 (GRCm39)	missense	probably benign	0.00
IGL02066:Vmn2r77	APN	7	86,452,836 (GRCm39)	nonsense	probably null
IGL02185:Vmn2r77	APN	7	86,444,360 (GRCm39)	missense	unknown
IGL02200:Vmn2r77	APN	7	86,451,187 (GRCm39)	missense	probably benign	0.04
IGL02336:Vmn2r77	APN	7	86,451,224 (GRCm39)	missense	probably damaging	0.99
IGL02445:Vmn2r77	APN	7	86,452,848 (GRCm39)	nonsense	probably null
IGL02557:Vmn2r77	APN	7	86,444,342 (GRCm39)	unclassified	probably benign
IGL02659:Vmn2r77	APN	7	86,449,979 (GRCm39)	missense	probably benign	0.32
IGL02978:Vmn2r77	APN	7	86,460,555 (GRCm39)	missense	probably benign
IGL03180:Vmn2r77	APN	7	86,450,843 (GRCm39)	missense	possibly damaging	0.85
IGL03255:Vmn2r77	APN	7	86,461,131 (GRCm39)	missense	probably benign	0.04
IGL03273:Vmn2r77	APN	7	86,460,494 (GRCm39)	missense	probably damaging	0.99
R0046:Vmn2r77	UTSW	7	86,451,146 (GRCm39)	missense	possibly damaging	0.73
R0047:Vmn2r77	UTSW	7	86,460,858 (GRCm39)	missense	probably benign	0.01
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0389:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R0635:Vmn2r77	UTSW	7	86,460,383 (GRCm39)	missense	probably benign
R0689:Vmn2r77	UTSW	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
R0827:Vmn2r77	UTSW	7	86,451,224 (GRCm39)	missense	probably damaging	1.00
R1167:Vmn2r77	UTSW	7	86,450,954 (GRCm39)	missense	probably benign	0.02
R1228:Vmn2r77	UTSW	7	86,450,242 (GRCm39)	critical splice donor site	probably null
R1353:Vmn2r77	UTSW	7	86,451,394 (GRCm39)	missense	probably benign	0.29
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1613:Vmn2r77	UTSW	7	86,460,356 (GRCm39)	missense	probably damaging	1.00
R1654:Vmn2r77	UTSW	7	86,461,123 (GRCm39)	missense	probably damaging	1.00
R1742:Vmn2r77	UTSW	7	86,444,543 (GRCm39)	missense	probably benign	0.35
R1827:Vmn2r77	UTSW	7	86,450,821 (GRCm39)	missense	probably damaging	0.99
R1911:Vmn2r77	UTSW	7	86,461,001 (GRCm39)	missense	probably damaging	1.00
R1974:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R2008:Vmn2r77	UTSW	7	86,450,921 (GRCm39)	missense	probably benign	0.31
R2093:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R2143:Vmn2r77	UTSW	7	86,461,152 (GRCm39)	missense	probably damaging	1.00
R2269:Vmn2r77	UTSW	7	86,460,897 (GRCm39)	missense	probably benign	0.03
R2972:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R2974:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R3037:Vmn2r77	UTSW	7	86,450,191 (GRCm39)	missense	probably benign
R3694:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3695:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3805:Vmn2r77	UTSW	7	86,444,368 (GRCm39)	nonsense	probably null
R3870:Vmn2r77	UTSW	7	86,461,050 (GRCm39)	missense	probably damaging	1.00
R4732:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R4733:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R5009:Vmn2r77	UTSW	7	86,451,015 (GRCm39)	missense	possibly damaging	0.82
R5201:Vmn2r77	UTSW	7	86,460,846 (GRCm39)	missense	probably damaging	0.98
R5218:Vmn2r77	UTSW	7	86,451,341 (GRCm39)	missense	probably damaging	0.98
R5673:Vmn2r77	UTSW	7	86,461,214 (GRCm39)	missense	probably benign	0.05
R5771:Vmn2r77	UTSW	7	86,461,235 (GRCm39)	missense	probably benign	0.06
R5832:Vmn2r77	UTSW	7	86,460,670 (GRCm39)	nonsense	probably null
R5899:Vmn2r77	UTSW	7	86,460,924 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn2r77	UTSW	7	86,450,878 (GRCm39)	missense	probably benign	0.00
R6182:Vmn2r77	UTSW	7	86,460,957 (GRCm39)	missense	probably damaging	1.00
R6326:Vmn2r77	UTSW	7	86,451,031 (GRCm39)	missense	probably benign
R6419:Vmn2r77	UTSW	7	86,460,767 (GRCm39)	missense	probably damaging	0.99
R6549:Vmn2r77	UTSW	7	86,450,065 (GRCm39)	missense	probably benign	0.06
R6874:Vmn2r77	UTSW	7	86,451,286 (GRCm39)	missense	probably benign	0.00
R6972:Vmn2r77	UTSW	7	86,452,202 (GRCm39)	missense	probably damaging	1.00
R7056:Vmn2r77	UTSW	7	86,451,023 (GRCm39)	missense	probably benign	0.06
R7185:Vmn2r77	UTSW	7	86,451,035 (GRCm39)	missense	probably benign	0.00
R7261:Vmn2r77	UTSW	7	86,460,518 (GRCm39)	nonsense	probably null
R7298:Vmn2r77	UTSW	7	86,449,979 (GRCm39)	missense	probably benign	0.00
R7662:Vmn2r77	UTSW	7	86,460,492 (GRCm39)	nonsense	probably null
R8182:Vmn2r77	UTSW	7	86,460,801 (GRCm39)	missense	probably damaging	1.00
R8327:Vmn2r77	UTSW	7	86,450,680 (GRCm39)	missense	probably benign	0.08
R8387:Vmn2r77	UTSW	7	86,450,947 (GRCm39)	missense	probably benign	0.00
R8825:Vmn2r77	UTSW	7	86,452,855 (GRCm39)	missense	probably benign
R8898:Vmn2r77	UTSW	7	86,444,430 (GRCm39)	missense	probably damaging	1.00
R8973:Vmn2r77	UTSW	7	86,452,150 (GRCm39)	missense	possibly damaging	0.93
R9258:Vmn2r77	UTSW	7	86,452,302 (GRCm39)	missense	possibly damaging	0.88
R9338:Vmn2r77	UTSW	7	86,460,994 (GRCm39)	missense	probably damaging	1.00
R9358:Vmn2r77	UTSW	7	86,452,236 (GRCm39)	missense	probably benign	0.00
R9377:Vmn2r77	UTSW	7	86,444,442 (GRCm39)	missense	probably benign	0.05
R9404:Vmn2r77	UTSW	7	86,451,247 (GRCm39)	missense	probably benign
R9673:Vmn2r77	UTSW	7	86,450,171 (GRCm39)	missense	possibly damaging	0.75
R9679:Vmn2r77	UTSW	7	86,460,741 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- GATCACAATGATGGGAGATTTCATGC -3'
(R):5'- TCCCATTAGGAATGTCGTTTCTATG -3'

Sequencing Primer
(F):5'- CACATCAACAGTCTGAGATGTCTGG -3'
(R):5'- CATTCTCTGAGGATGTGTC -3'

Posted On

2016-10-06