|Institutional Source||Beutler Lab|
|Gene Name||ubiquitin B|
|Is this an essential gene?||Possibly non essential (E-score: 0.313)|
|Stock #||R6753 (G1)|
|Chromosomal Location||62551171-62553213 bp(+) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||T to G at 62551527 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000019649 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000019649] [ENSMUST00000136938]|
|Predicted Effect||probably null
|Predicted Effect||probably benign
|Predicted Effect||noncoding transcript
|Coding Region Coverage||
|Validation Efficiency||99% (68/69)|
FUNCTION: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of four direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Pseudogenes of this gene are located on chromosomes 3 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
PHENOTYPE: Targeted disruption of this gene results in progressive degeneration of hypothalamic neurons accompanied by impaired hypothalamic control of energy balance and adult-onset obesity. Both genders are infertile due to a failure of germ cells to progress through meiosis I and hypogonadism. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ubb||
(F):5'- TCTGAAGAATCGGGAGCTCG -3'
(R):5'- TTAAGCGTCCGTTAAGCTCC -3'
(F):5'- TCGGGAGCTCGGCAAAAAC -3'
(R):5'- GTCCGTTAAGCTCCCTGGC -3'