Mutagenetix > Incidental Mutation

Incidental Mutation 'R7063:Vmn2r16'

548387

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r16

Ensembl Gene

ENSMUSG00000092080

Gene Name

vomeronasal 2, receptor 16

Synonyms

EG384220

MMRRC Submission

045159-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.154) question?

Stock #

R7063 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109478247-109512347 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 109511650 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 619 (N619S)

Ref Sequence

ENSEMBL: ENSMUSP00000127838 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165180]

AlphaFold

A0A3B2WCY4

Predicted Effect

probably damaging
Transcript: ENSMUST00000165180
AA Change: N619S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127838
Gene: ENSMUSG00000092080
AA Change: N619S

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	76	425	2.3e-28	PFAM
Pfam:NCD3G	509	563	8.2e-19	PFAM
Pfam:7tm_3	596	831	3.5e-56	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap35	A	G	7: 16,299,038 (GRCm39)	V9A	probably benign	Het
Btbd6	A	G	12: 112,941,132 (GRCm39)	Y148C	probably damaging	Het
Casr	T	C	16: 36,314,936 (GRCm39)	I968V	probably benign	Het
Celf1	T	C	2: 90,843,189 (GRCm39)		probably null	Het
Chst2	G	T	9: 95,287,621 (GRCm39)	R242S	probably benign	Het
Clca3a1	T	A	3: 144,460,967 (GRCm39)	D228V	probably damaging	Het
Cwf19l2	A	G	9: 3,430,532 (GRCm39)	Y288C	probably benign	Het
Cyp2r1	A	G	7: 114,152,184 (GRCm39)	S49P	probably damaging	Het
Dnah5	A	G	15: 28,233,394 (GRCm39)	E251G	probably benign	Het
Eepd1	A	G	9: 25,394,332 (GRCm39)	R199G	possibly damaging	Het
Fat1	C	T	8: 45,493,812 (GRCm39)	T3986I	probably benign	Het
Gbp4	G	A	5: 105,266,314 (GRCm39)	R576C	probably damaging	Het
Glp1r	A	G	17: 31,144,532 (GRCm39)	Y235C	probably damaging	Het
Gm6408	T	C	5: 146,420,594 (GRCm39)	I158T	probably benign	Het
Gpr89	C	G	3: 96,783,014 (GRCm39)	R312P	probably damaging	Het
Idh2	A	G	7: 79,745,432 (GRCm39)	V403A	probably damaging	Het
Lclat1	A	G	17: 73,546,986 (GRCm39)	E301G	possibly damaging	Het
Lgmn	A	G	12: 102,368,937 (GRCm39)	Y181H	probably damaging	Het
Lgr5	T	C	10: 115,292,639 (GRCm39)	Y417C	probably damaging	Het
Man1a	G	T	10: 53,906,840 (GRCm39)	N311K	probably damaging	Het
Nat10	G	A	2: 103,578,422 (GRCm39)	L228F	probably benign	Het
Or10d3	T	G	9: 39,461,411 (GRCm39)	Y252S	possibly damaging	Het
Or10v5	A	G	19: 11,805,548 (GRCm39)	F281L	probably damaging	Het
Or5b12b	A	G	19: 12,861,449 (GRCm39)	D68G	probably damaging	Het
Or5p55	C	A	7: 107,567,411 (GRCm39)	A269E	probably benign	Het
Pcmtd2	T	A	2: 181,496,776 (GRCm39)	Y130*	probably null	Het
Potefam3f	G	A	8: 20,479,013 (GRCm39)	C7Y		Het
Qrfprl	A	G	6: 65,418,387 (GRCm39)		probably benign	Het
Rapgef5	A	G	12: 117,652,864 (GRCm39)	D62G	possibly damaging	Het
Serpina3m	A	T	12: 104,357,726 (GRCm39)	I217L	probably benign	Het
Supt16	T	C	14: 52,409,505 (GRCm39)	R802G	possibly damaging	Het
Sypl2	A	T	3: 108,124,971 (GRCm39)	M130K	probably benign	Het
Tmtc2	A	T	10: 105,184,386 (GRCm39)	L503Q	probably damaging	Het
Ubald2	A	G	11: 116,325,443 (GRCm39)	Q60R	probably benign	Het
Vav1	C	T	17: 57,618,860 (GRCm39)	Q673*	probably null	Het
Vgll2	T	C	10: 51,904,072 (GRCm39)	S312P	probably benign	Het
Vmn1r191	C	T	13: 22,362,864 (GRCm39)	A297T	probably benign	Het
Vmn1r7	T	A	6: 57,001,418 (GRCm39)	I281F	possibly damaging	Het
Vmn2r108	T	C	17: 20,701,410 (GRCm39)	Y30C	probably damaging	Het
Vstm5	A	G	9: 15,150,549 (GRCm39)		probably benign	Het
Zfp7	A	G	15: 76,775,919 (GRCm39)	I654V	possibly damaging	Het
Zmat2	A	G	18: 36,929,627 (GRCm39)	N129S	probably null	Het

Other mutations in Vmn2r16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01116:Vmn2r16	APN	5	109,488,294 (GRCm39)	missense	probably damaging	1.00
IGL01374:Vmn2r16	APN	5	109,478,283 (GRCm39)	missense	probably benign	0.00
IGL01391:Vmn2r16	APN	5	109,511,627 (GRCm39)	missense	possibly damaging	0.50
IGL01419:Vmn2r16	APN	5	109,510,267 (GRCm39)	splice site	probably benign
IGL01634:Vmn2r16	APN	5	109,488,177 (GRCm39)	missense	probably benign	0.00
IGL01845:Vmn2r16	APN	5	109,511,762 (GRCm39)	missense	probably damaging	1.00
IGL01875:Vmn2r16	APN	5	109,478,277 (GRCm39)	missense	probably benign	0.01
IGL01910:Vmn2r16	APN	5	109,487,951 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vmn2r16	APN	5	109,487,676 (GRCm39)	missense	probably damaging	0.98
IGL02327:Vmn2r16	APN	5	109,487,987 (GRCm39)	missense	probably benign	0.01
IGL02491:Vmn2r16	APN	5	109,487,703 (GRCm39)	nonsense	probably null
IGL02531:Vmn2r16	APN	5	109,488,134 (GRCm39)	missense	probably damaging	0.99
IGL02680:Vmn2r16	APN	5	109,487,948 (GRCm39)	missense	probably benign	0.44
IGL02884:Vmn2r16	APN	5	109,508,757 (GRCm39)	missense	possibly damaging	0.94
IGL03084:Vmn2r16	APN	5	109,478,292 (GRCm39)	missense	probably damaging	0.99
IGL03096:Vmn2r16	APN	5	109,487,751 (GRCm39)	missense	probably damaging	0.99
IGL03355:Vmn2r16	APN	5	109,511,580 (GRCm39)	missense	possibly damaging	0.74
R0280:Vmn2r16	UTSW	5	109,488,005 (GRCm39)	missense	possibly damaging	0.88
R0594:Vmn2r16	UTSW	5	109,511,762 (GRCm39)	missense	probably damaging	1.00
R1016:Vmn2r16	UTSW	5	109,487,754 (GRCm39)	missense	probably damaging	1.00
R1109:Vmn2r16	UTSW	5	109,487,652 (GRCm39)	missense	probably damaging	0.98
R1248:Vmn2r16	UTSW	5	109,508,643 (GRCm39)	missense	probably benign	0.10
R1626:Vmn2r16	UTSW	5	109,511,443 (GRCm39)	missense	probably damaging	1.00
R1909:Vmn2r16	UTSW	5	109,511,853 (GRCm39)	missense	probably benign	0.01
R1929:Vmn2r16	UTSW	5	109,487,124 (GRCm39)	missense	possibly damaging	0.92
R1982:Vmn2r16	UTSW	5	109,511,890 (GRCm39)	missense	probably benign	0.01
R3038:Vmn2r16	UTSW	5	109,487,199 (GRCm39)	missense	probably damaging	1.00
R3437:Vmn2r16	UTSW	5	109,478,362 (GRCm39)	missense	probably damaging	0.99
R3734:Vmn2r16	UTSW	5	109,478,280 (GRCm39)	missense	probably benign	0.11
R3820:Vmn2r16	UTSW	5	109,510,143 (GRCm39)	missense	probably benign	0.36
R3873:Vmn2r16	UTSW	5	109,488,177 (GRCm39)	missense	probably benign	0.33
R4165:Vmn2r16	UTSW	5	109,478,427 (GRCm39)	missense	possibly damaging	0.80
R4373:Vmn2r16	UTSW	5	109,511,667 (GRCm39)	missense	probably damaging	1.00
R4575:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4576:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4578:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4637:Vmn2r16	UTSW	5	109,478,280 (GRCm39)	missense	probably benign	0.00
R4696:Vmn2r16	UTSW	5	109,487,168 (GRCm39)	missense	probably benign	0.01
R5026:Vmn2r16	UTSW	5	109,508,722 (GRCm39)	nonsense	probably null
R5180:Vmn2r16	UTSW	5	109,478,391 (GRCm39)	missense	probably benign	0.01
R5433:Vmn2r16	UTSW	5	109,511,708 (GRCm39)	missense	probably damaging	1.00
R5955:Vmn2r16	UTSW	5	109,511,613 (GRCm39)	missense	possibly damaging	0.78
R5958:Vmn2r16	UTSW	5	109,510,153 (GRCm39)	missense	possibly damaging	0.81
R6353:Vmn2r16	UTSW	5	109,488,119 (GRCm39)	missense	probably benign	0.33
R6389:Vmn2r16	UTSW	5	109,478,344 (GRCm39)	missense	probably benign	0.19
R6819:Vmn2r16	UTSW	5	109,488,412 (GRCm39)	missense	probably benign	0.04
R6994:Vmn2r16	UTSW	5	109,487,969 (GRCm39)	missense	probably damaging	1.00
R7061:Vmn2r16	UTSW	5	109,511,620 (GRCm39)	missense	probably damaging	0.99
R7220:Vmn2r16	UTSW	5	109,508,772 (GRCm39)	missense	probably damaging	0.97
R7268:Vmn2r16	UTSW	5	109,488,331 (GRCm39)	nonsense	probably null
R7420:Vmn2r16	UTSW	5	109,511,736 (GRCm39)	missense	probably damaging	0.96
R7591:Vmn2r16	UTSW	5	109,510,223 (GRCm39)	missense	probably damaging	0.99
R7644:Vmn2r16	UTSW	5	109,487,837 (GRCm39)	missense	probably damaging	1.00
R7939:Vmn2r16	UTSW	5	109,487,705 (GRCm39)	missense	possibly damaging	0.79
R7977:Vmn2r16	UTSW	5	109,488,015 (GRCm39)	missense	probably damaging	1.00
R7987:Vmn2r16	UTSW	5	109,488,015 (GRCm39)	missense	probably damaging	1.00
R8023:Vmn2r16	UTSW	5	109,488,272 (GRCm39)	nonsense	probably null
R8427:Vmn2r16	UTSW	5	109,488,138 (GRCm39)	missense	probably benign	0.03
R8436:Vmn2r16	UTSW	5	109,511,649 (GRCm39)	missense	probably damaging	1.00
R8475:Vmn2r16	UTSW	5	109,487,073 (GRCm39)	missense	probably benign	0.01
R8554:Vmn2r16	UTSW	5	109,511,997 (GRCm39)	missense	probably benign	0.13
R8771:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8774:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8774-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8775:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8775-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8777:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8777-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8825:Vmn2r16	UTSW	5	109,487,019 (GRCm39)	missense	probably benign	0.41
R8865:Vmn2r16	UTSW	5	109,487,910 (GRCm39)	missense	probably benign	0.33
R8900:Vmn2r16	UTSW	5	109,511,619 (GRCm39)	missense	probably benign	0.04
R9365:Vmn2r16	UTSW	5	109,488,064 (GRCm39)	missense	probably damaging	0.99
R9683:Vmn2r16	UTSW	5	109,511,677 (GRCm39)	missense	probably damaging	1.00
X0027:Vmn2r16	UTSW	5	109,487,175 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r16	UTSW	5	109,511,779 (GRCm39)	frame shift	probably null
Z1088:Vmn2r16	UTSW	5	109,488,381 (GRCm39)	missense	probably benign	0.03
Z1177:Vmn2r16	UTSW	5	109,487,864 (GRCm39)	missense	possibly damaging	0.79

Predicted Primers

PCR Primer
(F):5'- TTTATGAGCAGATATGGAGCAGTG -3'
(R):5'- GCTTGAAAGCCATGACCACAG -3'

Sequencing Primer
(F):5'- GGTGTCCAGATGATAAATATGCC -3'
(R):5'- GACCACAGTTATTGTTTTGGCCAAC -3'

Posted On

2019-05-13