Mutagenetix > Incidental Mutation

Incidental Mutation 'R7326:Akap9'

568881

Institutional Source

Beutler Lab

Gene Symbol

Akap9

Ensembl Gene

ENSMUSG00000040407

Gene Name

A kinase (PRKA) anchor protein (yotiao) 9

Synonyms

AKAP450, G1-448-15, 5730481H23Rik, mei2-5, repro12

MMRRC Submission

045378-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.327) question?

Stock #

R7326 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

3928054-4081310 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 4045930 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 2268 (D2268E)

Ref Sequence

ENSEMBL: ENSMUSP00000046129 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044492]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000044492
AA Change: D2268E

PolyPhen 2 Score 0.466 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000046129
Gene: ENSMUSG00000040407
AA Change: D2268E

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	98	115	N/A	INTRINSIC
Blast:HPT	126	197	6e-21	BLAST
low complexity region	237	249	N/A	INTRINSIC
low complexity region	297	315	N/A	INTRINSIC
coiled coil region	404	593	N/A	INTRINSIC
coiled coil region	622	756	N/A	INTRINSIC
coiled coil region	777	843	N/A	INTRINSIC
coiled coil region	888	958	N/A	INTRINSIC
low complexity region	982	997	N/A	INTRINSIC
coiled coil region	1037	1065	N/A	INTRINSIC
low complexity region	1233	1246	N/A	INTRINSIC
internal_repeat_2	1247	1312	7.75e-5	PROSPERO
internal_repeat_1	1377	1485	2.63e-5	PROSPERO
coiled coil region	1522	1589	N/A	INTRINSIC
coiled coil region	1789	2107	N/A	INTRINSIC
coiled coil region	2132	2318	N/A	INTRINSIC
internal_repeat_1	2322	2445	2.63e-5	PROSPERO
coiled coil region	2455	2494	N/A	INTRINSIC
low complexity region	2587	2598	N/A	INTRINSIC
low complexity region	2627	2640	N/A	INTRINSIC
internal_repeat_2	2934	2997	7.75e-5	PROSPERO
low complexity region	3000	3016	N/A	INTRINSIC
coiled coil region	3109	3307	N/A	INTRINSIC
coiled coil region	3455	3493	N/A	INTRINSIC
coiled coil region	3521	3556	N/A	INTRINSIC
Pfam:PACT_coil_coil	3576	3657	1.2e-27	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a chemically induced allele exhibit male infertily with abnormal spermatogenesis and Sertoli maturation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 112 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430531B16Rik	A	T	7: 139,978,545 (GRCm38)		probably null	Het
Abcb4	T	C	5: 8,934,226 (GRCm38)	V652A	probably benign	Het
Acvrl1	G	T	15: 101,141,072 (GRCm38)	V417L	probably damaging	Het
Add1	A	G	5: 34,619,371 (GRCm38)	R479G	probably benign	Het
Adgb	A	T	10: 10,400,574 (GRCm38)	L350Q	possibly damaging	Het
Adgrl2	A	T	3: 148,846,870 (GRCm38)	S666T	probably benign	Het
Amigo3	C	A	9: 108,054,066 (GRCm38)	H229Q	probably benign	Het
Ano6	G	C	15: 95,864,244 (GRCm38)	Q31H	possibly damaging	Het
Ap4m1	G	A	5: 138,175,019 (GRCm38)	D180N	probably damaging	Het
Apc2	A	T	10: 80,311,740 (GRCm38)	D876V	probably damaging	Het
Apcdd1	T	A	18: 62,952,188 (GRCm38)	Y485*	probably null	Het
Aqp1	G	T	6: 55,336,851 (GRCm38)	D121Y	probably benign	Het
Bicd2	C	A	13: 49,369,609 (GRCm38)	R141S	probably benign	Het
Brpf3	C	A	17: 28,806,293 (GRCm38)	H113Q	probably benign	Het
Cacng2	A	T	15: 78,013,320 (GRCm38)	Y96*	probably null	Het
Catsperg1	G	T	7: 29,210,759 (GRCm38)	N52K	possibly damaging	Het
Celsr2	A	C	3: 108,394,995 (GRCm38)	F2606V	possibly damaging	Het
Cerkl	A	T	2: 79,332,605 (GRCm38)	N516K	probably benign	Het
Ciita	G	A	16: 10,512,288 (GRCm38)	R812H	probably damaging	Het
Cldn3	T	A	5: 134,986,983 (GRCm38)	L180Q	probably damaging	Het
Cnot6l	T	C	5: 96,077,299 (GRCm38)	I512V	probably benign	Het
Col5a2	A	T	1: 45,442,867 (GRCm38)	D32E	unknown	Het
Coro7	A	G	16: 4,632,048 (GRCm38)	V616A	probably damaging	Het
Cyp4f18	T	C	8: 71,988,654 (GRCm38)	D494G	probably benign	Het
Ddx39	T	A	8: 83,722,471 (GRCm38)	V296E	probably benign	Het
Dgkg	A	T	16: 22,548,690 (GRCm38)	H593Q	probably damaging	Het
Dhx16	T	C	17: 35,886,160 (GRCm38)	L645P	probably damaging	Het
Dnah14	A	G	1: 181,598,403 (GRCm38)	M171V	probably benign	Het
Dnhd1	G	A	7: 105,720,930 (GRCm38)	V4521I	probably damaging	Het
Dok7	A	T	5: 35,064,522 (GRCm38)	M60L	probably benign	Het
Donson	A	T	16: 91,688,711 (GRCm38)	M1K	probably null	Het
Dsp	C	T	13: 38,192,883 (GRCm38)	T1548M	probably benign	Het
Dyrk1a	A	G	16: 94,692,043 (GRCm38)	T712A	probably damaging	Het
Eef2	A	G	10: 81,181,282 (GRCm38)	T708A	probably benign	Het
Epn3	G	A	11: 94,493,780 (GRCm38)	T289I	probably benign	Het
Fam171a1	C	T	2: 3,226,472 (GRCm38)	R881*	probably null	Het
Fam71a	G	A	1: 191,164,353 (GRCm38)	T31M	probably benign	Het
Fat2	T	A	11: 55,282,304 (GRCm38)	R2528W	probably damaging	Het
Fgfr1	T	G	8: 25,573,839 (GRCm38)	F707C	probably damaging	Het
Frem2	G	C	3: 53,654,753 (GRCm38)	P778A	probably damaging	Het
Ganc	A	G	2: 120,430,599 (GRCm38)	Y255C	probably damaging	Het
Ginm1	A	T	10: 7,777,850 (GRCm38)	Y65*	probably null	Het
Gm11559	T	A	11: 99,864,881 (GRCm38)	C119S	unknown	Het
Gnai1	T	A	5: 18,289,551 (GRCm38)	H188L		Het
H6pd	C	T	4: 149,996,350 (GRCm38)	A13T	probably benign	Het
Hist1h2be	T	C	13: 23,585,923 (GRCm38)	K12E	probably benign	Het
Hoxd12	A	T	2: 74,675,246 (GRCm38)	T54S	possibly damaging	Het
Hs3st5	T	A	10: 36,833,194 (GRCm38)	L242M	probably damaging	Het
Il9r	C	A	11: 32,194,389 (GRCm38)	V139L	possibly damaging	Het
Immt	A	G	6: 71,846,369 (GRCm38)	D68G	probably damaging	Het
Itsn2	A	G	12: 4,632,985 (GRCm38)	I304V	unknown	Het
Lgr4	G	A	2: 109,996,629 (GRCm38)	W159*	probably null	Het
Lin52	G	A	12: 84,457,954 (GRCm38)	G38S	probably damaging	Het
Lpgat1	A	T	1: 191,719,453 (GRCm38)	M64L	probably benign	Het
Lrrc10b	G	T	19: 10,456,778 (GRCm38)	R180S	possibly damaging	Het
Lrrc29	T	C	8: 105,312,898 (GRCm38)	D127G	probably damaging	Het
Lrrc36	A	T	8: 105,449,769 (GRCm38)	L258F	possibly damaging	Het
Ltbp4	T	A	7: 27,329,755 (GRCm38)	Q235L	unknown	Het
Maml2	A	G	9: 13,621,607 (GRCm38)	M706V		Het
Mc5r	G	T	18: 68,339,668 (GRCm38)	C366F	probably damaging	Het
Mlip	T	C	9: 77,164,842 (GRCm38)	R244G	probably benign	Het
Muc16	T	A	9: 18,585,013 (GRCm38)	R6658S	probably benign	Het
Mup4	G	T	4: 59,960,046 (GRCm38)	H73N	possibly damaging	Het
Nf1	A	G	11: 79,564,943 (GRCm38)	M565V	probably benign	Het
Nphp1	G	A	2: 127,761,217 (GRCm38)	T382I	possibly damaging	Het
Nrcam	C	T	12: 44,564,026 (GRCm38)	T503I	possibly damaging	Het
Nwd2	A	T	5: 63,800,409 (GRCm38)	N361Y	probably damaging	Het
Olfr1045	A	G	2: 86,198,573 (GRCm38)	Y60H	probably damaging	Het
Olfr1309	A	T	2: 111,983,327 (GRCm38)	L249*	probably null	Het
Olfr308	A	G	7: 86,321,574 (GRCm38)	I126T	probably damaging	Het
Olfr461	G	T	6: 40,544,895 (GRCm38)	A28E	probably damaging	Het
Olfr518	A	T	7: 108,880,816 (GRCm38)	H263Q	probably damaging	Het
Olfr836	A	G	9: 19,121,669 (GRCm38)	Y235C	probably benign	Het
Olfr993	A	T	2: 85,414,444 (GRCm38)	V145E	probably damaging	Het
Orc5	A	G	5: 22,523,584 (GRCm38)	F308L	probably benign	Het
Padi1	T	A	4: 140,832,404 (GRCm38)	Y54F	probably benign	Het
Parp1	A	G	1: 180,569,100 (GRCm38)	K23E	possibly damaging	Het
Pate1	A	T	9: 35,685,972 (GRCm38)	V79D	possibly damaging	Het
Pcdh18	T	C	3: 49,756,860 (GRCm38)	H2R	probably benign	Het
Pcnx2	G	A	8: 125,887,083 (GRCm38)	S543F	probably damaging	Het
Pcp4l1	G	A	1: 171,174,465 (GRCm38)	A42V	possibly damaging	Het
Pnkp	T	A	7: 44,859,734 (GRCm38)	F169L	probably damaging	Het
Ppp6r3	A	T	19: 3,507,325 (GRCm38)	N254K	probably damaging	Het
Psd	A	G	19: 46,324,454 (GRCm38)	L159P	probably benign	Het
Ptprf	A	G	4: 118,231,669 (GRCm38)	Y646H	probably damaging	Het
Rab3ip	A	T	10: 116,937,633 (GRCm38)	S92T	probably benign	Het
Rabgef1	A	G	5: 130,187,351 (GRCm38)		probably benign	Het
Ralgapa1	C	A	12: 55,709,004 (GRCm38)	W1129L	probably damaging	Het
Rhpn2	T	A	7: 35,385,463 (GRCm38)	L594Q	probably benign	Het
Rnf38	C	A	4: 44,158,989 (GRCm38)		probably benign	Het
Ryr2	T	A	13: 11,738,194 (GRCm38)	H1747L	possibly damaging	Het
Sec16a	A	G	2: 26,439,717 (GRCm38)	L13P	unknown	Het
Sppl3	C	A	5: 115,082,335 (GRCm38)	T102K	probably damaging	Het
Srgap2	A	T	1: 131,291,613 (GRCm38)	Y264*	probably null	Het
Stxbp2	T	C	8: 3,641,151 (GRCm38)	M465T		Het
Sumf2	A	G	5: 129,862,710 (GRCm38)	K305R	probably benign	Het
Susd2	T	A	10: 75,642,565 (GRCm38)	Y59F	probably benign	Het
Taco1	T	C	11: 106,072,617 (GRCm38)	V198A	probably benign	Het
Tas2r136	A	T	6: 132,777,906 (GRCm38)	M86K	possibly damaging	Het
Tbc1d10c	T	C	19: 4,184,898 (GRCm38)	E388G	possibly damaging	Het
Tmem132c	C	T	5: 127,564,059 (GRCm38)	T1098I	possibly damaging	Het
Trim3	G	T	7: 105,617,800 (GRCm38)	Y457*	probably null	Het
Ttc38	A	G	15: 85,852,861 (GRCm38)	T316A	probably benign	Het
Ubqln4	T	A	3: 88,555,910 (GRCm38)	N127K	probably benign	Het
Wdr91	A	T	6: 34,904,626 (GRCm38)	F262Y	probably damaging	Het
Zdhhc6	A	T	19: 55,302,755 (GRCm38)	C343S	possibly damaging	Het
Zfp248	A	G	6: 118,430,209 (GRCm38)	C140R	probably damaging	Het
Zfp266	T	C	9: 20,502,095 (GRCm38)	T90A	probably benign	Het
Zfp407	A	T	18: 84,559,042 (GRCm38)	D1315E	possibly damaging	Het
Zfp644	T	C	5: 106,638,277 (GRCm38)	S135G	probably benign	Het
Zscan29	A	T	2: 121,160,988 (GRCm38)	I773N	probably damaging	Het
Zswim5	T	A	4: 116,980,834 (GRCm38)	V787E	possibly damaging	Het

Other mutations in Akap9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00478:Akap9	APN	5	4,046,639 (GRCm38)	missense	probably damaging	0.97
IGL00642:Akap9	APN	5	3,960,842 (GRCm38)	missense	probably damaging	0.99
IGL00786:Akap9	APN	5	4,070,522 (GRCm38)	missense	probably damaging	1.00
IGL00788:Akap9	APN	5	4,060,480 (GRCm38)	missense	probably damaging	1.00
IGL00969:Akap9	APN	5	4,001,550 (GRCm38)	missense	probably benign
IGL01014:Akap9	APN	5	3,968,683 (GRCm38)	missense	probably benign	0.41
IGL01302:Akap9	APN	5	3,970,711 (GRCm38)	missense	probably benign	0.27
IGL01610:Akap9	APN	5	4,032,839 (GRCm38)	missense	possibly damaging	0.95
IGL01620:Akap9	APN	5	3,960,218 (GRCm38)	missense	probably benign	0.11
IGL01862:Akap9	APN	5	4,065,856 (GRCm38)	missense	probably damaging	0.99
IGL01862:Akap9	APN	5	3,951,705 (GRCm38)	missense	probably damaging	0.99
IGL02151:Akap9	APN	5	4,032,728 (GRCm38)	nonsense	probably null
IGL02635:Akap9	APN	5	4,070,500 (GRCm38)	missense	possibly damaging	0.59
IGL02858:Akap9	APN	5	4,069,130 (GRCm38)	missense	possibly damaging	0.88
IGL02967:Akap9	APN	5	3,976,164 (GRCm38)	missense	probably benign	0.07
IGL03064:Akap9	APN	5	3,968,755 (GRCm38)	missense	probably damaging	1.00
IGL03289:Akap9	APN	5	4,077,261 (GRCm38)	missense	probably damaging	1.00
Andy	UTSW	5	3,961,764 (GRCm38)	nonsense	probably null
blimey	UTSW	5	4,070,397 (GRCm38)	nonsense	probably null
hoarder	UTSW	5	4,069,089 (GRCm38)	missense	probably benign	0.00
marinarum	UTSW	5	4,013,875 (GRCm38)	nonsense	probably null
miser	UTSW	5	4,046,064 (GRCm38)	missense	probably benign	0.13
naviculus	UTSW	5	3,960,865 (GRCm38)	missense	probably damaging	0.98
thrifty	UTSW	5	3,976,209 (GRCm38)	missense	probably damaging	0.99
wee_one	UTSW	5	4,043,925 (GRCm38)	missense	probably damaging	1.00
FR4449:Akap9	UTSW	5	3,981,214 (GRCm38)	unclassified	probably benign
PIT1430001:Akap9	UTSW	5	4,029,849 (GRCm38)	missense	probably damaging	1.00
PIT4366001:Akap9	UTSW	5	4,046,221 (GRCm38)	missense	probably benign	0.24
R0088:Akap9	UTSW	5	3,961,946 (GRCm38)	missense	probably benign	0.22
R0309:Akap9	UTSW	5	4,069,038 (GRCm38)	missense	probably benign	0.01
R0387:Akap9	UTSW	5	3,951,678 (GRCm38)	splice site	probably benign
R0440:Akap9	UTSW	5	4,064,569 (GRCm38)	missense	probably damaging	0.99
R0441:Akap9	UTSW	5	3,961,714 (GRCm38)	missense	probably benign	0.15
R0491:Akap9	UTSW	5	3,972,851 (GRCm38)	unclassified	probably benign
R0501:Akap9	UTSW	5	3,970,685 (GRCm38)	missense	probably damaging	1.00
R0507:Akap9	UTSW	5	4,069,043 (GRCm38)	missense	probably benign	0.41
R0544:Akap9	UTSW	5	4,069,185 (GRCm38)	missense	probably benign	0.22
R0581:Akap9	UTSW	5	4,050,620 (GRCm38)	missense	probably benign	0.03
R0611:Akap9	UTSW	5	3,954,870 (GRCm38)	missense	probably benign	0.00
R0620:Akap9	UTSW	5	4,064,136 (GRCm38)	missense	probably damaging	0.98
R0639:Akap9	UTSW	5	4,060,318 (GRCm38)	missense	probably damaging	1.00
R0932:Akap9	UTSW	5	4,046,492 (GRCm38)	missense	possibly damaging	0.77
R0944:Akap9	UTSW	5	4,064,742 (GRCm38)	splice site	probably null
R1101:Akap9	UTSW	5	4,046,205 (GRCm38)	missense	probably benign	0.00
R1159:Akap9	UTSW	5	3,960,865 (GRCm38)	missense	probably damaging	0.98
R1170:Akap9	UTSW	5	4,055,671 (GRCm38)	missense	probably benign
R1185:Akap9	UTSW	5	3,948,783 (GRCm38)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,948,783 (GRCm38)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,948,783 (GRCm38)	missense	probably benign	0.13
R1453:Akap9	UTSW	5	3,975,614 (GRCm38)	splice site	probably null
R1551:Akap9	UTSW	5	4,069,174 (GRCm38)	missense	probably benign	0.02
R1608:Akap9	UTSW	5	3,961,783 (GRCm38)	missense	probably damaging	1.00
R1652:Akap9	UTSW	5	4,077,210 (GRCm38)	missense	probably damaging	1.00
R1659:Akap9	UTSW	5	4,064,633 (GRCm38)	missense	probably damaging	1.00
R1713:Akap9	UTSW	5	4,039,345 (GRCm38)	critical splice donor site	probably null
R1719:Akap9	UTSW	5	3,957,645 (GRCm38)	nonsense	probably null
R1720:Akap9	UTSW	5	3,972,791 (GRCm38)	missense	possibly damaging	0.63
R1757:Akap9	UTSW	5	4,001,667 (GRCm38)	missense	probably benign	0.41
R1872:Akap9	UTSW	5	4,001,406 (GRCm38)	missense	probably damaging	1.00
R1876:Akap9	UTSW	5	3,961,809 (GRCm38)	missense	probably benign	0.28
R1881:Akap9	UTSW	5	4,050,173 (GRCm38)	missense	probably benign
R1950:Akap9	UTSW	5	3,960,677 (GRCm38)	missense	probably damaging	1.00
R1980:Akap9	UTSW	5	3,972,771 (GRCm38)	missense	probably damaging	0.99
R1993:Akap9	UTSW	5	4,038,520 (GRCm38)	splice site	probably null
R2008:Akap9	UTSW	5	3,960,131 (GRCm38)	missense	possibly damaging	0.47
R2020:Akap9	UTSW	5	3,961,967 (GRCm38)	missense	probably damaging	1.00
R2051:Akap9	UTSW	5	3,975,685 (GRCm38)	nonsense	probably null
R2061:Akap9	UTSW	5	3,961,010 (GRCm38)	missense	probably damaging	1.00
R2109:Akap9	UTSW	5	4,044,847 (GRCm38)	missense	possibly damaging	0.47
R2135:Akap9	UTSW	5	4,064,509 (GRCm38)	missense	probably damaging	1.00
R2225:Akap9	UTSW	5	4,077,271 (GRCm38)	missense	probably damaging	0.96
R2232:Akap9	UTSW	5	4,046,603 (GRCm38)	missense	probably damaging	1.00
R2424:Akap9	UTSW	5	4,065,279 (GRCm38)	missense	probably damaging	0.97
R2483:Akap9	UTSW	5	3,976,235 (GRCm38)	missense	possibly damaging	0.65
R2879:Akap9	UTSW	5	3,976,353 (GRCm38)	intron	probably benign
R3622:Akap9	UTSW	5	3,976,235 (GRCm38)	missense	possibly damaging	0.65
R3623:Akap9	UTSW	5	3,976,235 (GRCm38)	missense	possibly damaging	0.65
R3624:Akap9	UTSW	5	3,976,235 (GRCm38)	missense	possibly damaging	0.65
R3722:Akap9	UTSW	5	4,070,351 (GRCm38)	missense	probably damaging	1.00
R3806:Akap9	UTSW	5	3,954,410 (GRCm38)	missense	probably benign	0.00
R3919:Akap9	UTSW	5	3,961,764 (GRCm38)	nonsense	probably null
R4023:Akap9	UTSW	5	3,992,077 (GRCm38)	missense	possibly damaging	0.66
R4093:Akap9	UTSW	5	4,043,996 (GRCm38)	missense	probably damaging	0.99
R4434:Akap9	UTSW	5	4,032,708 (GRCm38)	missense	probably damaging	0.99
R4529:Akap9	UTSW	5	4,043,948 (GRCm38)	missense	probably damaging	1.00
R4530:Akap9	UTSW	5	4,043,948 (GRCm38)	missense	probably damaging	1.00
R4532:Akap9	UTSW	5	4,043,948 (GRCm38)	missense	probably damaging	1.00
R4533:Akap9	UTSW	5	4,043,948 (GRCm38)	missense	probably damaging	1.00
R4585:Akap9	UTSW	5	3,976,151 (GRCm38)	missense	probably benign	0.00
R4586:Akap9	UTSW	5	3,976,151 (GRCm38)	missense	probably benign	0.00
R4655:Akap9	UTSW	5	4,046,403 (GRCm38)	missense	probably benign	0.14
R4676:Akap9	UTSW	5	4,064,515 (GRCm38)	nonsense	probably null
R4676:Akap9	UTSW	5	4,032,774 (GRCm38)	missense	probably damaging	1.00
R4724:Akap9	UTSW	5	4,055,339 (GRCm38)	missense	probably benign
R4731:Akap9	UTSW	5	3,962,266 (GRCm38)	missense	possibly damaging	0.54
R4732:Akap9	UTSW	5	4,013,901 (GRCm38)	missense	probably damaging	0.98
R4733:Akap9	UTSW	5	4,013,901 (GRCm38)	missense	probably damaging	0.98
R4743:Akap9	UTSW	5	3,961,013 (GRCm38)	missense	probably damaging	1.00
R4749:Akap9	UTSW	5	3,968,737 (GRCm38)	missense	probably benign	0.41
R4756:Akap9	UTSW	5	4,001,418 (GRCm38)	missense	probably damaging	0.99
R4757:Akap9	UTSW	5	4,008,382 (GRCm38)	missense	probably damaging	1.00
R4860:Akap9	UTSW	5	4,034,916 (GRCm38)	intron	probably benign
R4937:Akap9	UTSW	5	4,050,145 (GRCm38)	splice site	probably null
R4960:Akap9	UTSW	5	3,957,664 (GRCm38)	missense	probably benign	0.15
R4974:Akap9	UTSW	5	3,961,466 (GRCm38)	missense	possibly damaging	0.81
R5101:Akap9	UTSW	5	4,001,748 (GRCm38)	missense	probably damaging	0.96
R5160:Akap9	UTSW	5	4,030,007 (GRCm38)	missense	probably damaging	1.00
R5200:Akap9	UTSW	5	3,960,734 (GRCm38)	missense	probably benign	0.00
R5245:Akap9	UTSW	5	3,976,209 (GRCm38)	missense	probably damaging	0.99
R5293:Akap9	UTSW	5	3,948,687 (GRCm38)	missense	probably damaging	0.99
R5408:Akap9	UTSW	5	4,058,458 (GRCm38)	missense	possibly damaging	0.84
R5507:Akap9	UTSW	5	3,968,683 (GRCm38)	missense	probably benign	0.41
R5517:Akap9	UTSW	5	4,001,665 (GRCm38)	missense	possibly damaging	0.76
R5579:Akap9	UTSW	5	4,064,714 (GRCm38)	missense	possibly damaging	0.93
R5619:Akap9	UTSW	5	3,954,760 (GRCm38)	intron	probably benign
R5645:Akap9	UTSW	5	4,050,590 (GRCm38)	missense	probably benign	0.09
R5669:Akap9	UTSW	5	4,050,540 (GRCm38)	nonsense	probably null
R5686:Akap9	UTSW	5	3,971,926 (GRCm38)	missense	probably benign	0.00
R5697:Akap9	UTSW	5	3,960,170 (GRCm38)	missense	possibly damaging	0.92
R5821:Akap9	UTSW	5	4,046,064 (GRCm38)	missense	probably benign	0.13
R5875:Akap9	UTSW	5	4,077,285 (GRCm38)	missense	probably benign	0.01
R5897:Akap9	UTSW	5	4,077,904 (GRCm38)	missense	probably benign	0.23
R5999:Akap9	UTSW	5	4,043,925 (GRCm38)	missense	probably damaging	1.00
R6025:Akap9	UTSW	5	4,032,801 (GRCm38)	missense	probably damaging	1.00
R6078:Akap9	UTSW	5	4,067,924 (GRCm38)	critical splice donor site	probably null
R6138:Akap9	UTSW	5	4,067,924 (GRCm38)	critical splice donor site	probably null
R6225:Akap9	UTSW	5	3,962,105 (GRCm38)	missense	probably damaging	1.00
R6243:Akap9	UTSW	5	4,065,000 (GRCm38)	splice site	probably null
R6326:Akap9	UTSW	5	3,962,061 (GRCm38)	missense	probably damaging	1.00
R6564:Akap9	UTSW	5	4,028,491 (GRCm38)	missense	probably damaging	0.98
R6617:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R6625:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R6632:Akap9	UTSW	5	4,013,842 (GRCm38)	splice site	probably null
R6677:Akap9	UTSW	5	4,029,869 (GRCm38)	missense	probably benign	0.21
R6717:Akap9	UTSW	5	4,064,086 (GRCm38)	missense	probably damaging	1.00
R6893:Akap9	UTSW	5	3,961,709 (GRCm38)	missense	probably benign	0.32
R6915:Akap9	UTSW	5	3,960,551 (GRCm38)	missense	probably benign	0.03
R6938:Akap9	UTSW	5	4,046,628 (GRCm38)	missense	possibly damaging	0.91
R6972:Akap9	UTSW	5	4,046,699 (GRCm38)	missense	possibly damaging	0.62
R6973:Akap9	UTSW	5	4,046,699 (GRCm38)	missense	possibly damaging	0.62
R6993:Akap9	UTSW	5	4,065,866 (GRCm38)	missense	possibly damaging	0.65
R7032:Akap9	UTSW	5	3,954,896 (GRCm38)	missense	probably benign
R7164:Akap9	UTSW	5	4,060,364 (GRCm38)	missense	probably damaging	0.96
R7170:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7192:Akap9	UTSW	5	4,005,723 (GRCm38)	splice site	probably null
R7284:Akap9	UTSW	5	3,956,246 (GRCm38)	missense	probably damaging	1.00
R7299:Akap9	UTSW	5	4,032,696 (GRCm38)	missense	probably damaging	1.00
R7313:Akap9	UTSW	5	4,004,933 (GRCm38)	missense	probably damaging	1.00
R7343:Akap9	UTSW	5	4,046,364 (GRCm38)	missense	probably damaging	0.99
R7455:Akap9	UTSW	5	3,972,792 (GRCm38)	missense	probably benign	0.03
R7482:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7489:Akap9	UTSW	5	4,004,933 (GRCm38)	missense	probably damaging	1.00
R7525:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7528:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7576:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7577:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7578:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7610:Akap9	UTSW	5	3,957,677 (GRCm38)	missense	possibly damaging	0.95
R7658:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7754:Akap9	UTSW	5	4,046,736 (GRCm38)	missense	probably benign	0.03
R7818:Akap9	UTSW	5	4,013,875 (GRCm38)	nonsense	probably null
R7979:Akap9	UTSW	5	4,050,381 (GRCm38)	missense	probably benign
R7991:Akap9	UTSW	5	4,064,949 (GRCm38)	splice site	probably null
R8036:Akap9	UTSW	5	4,070,397 (GRCm38)	nonsense	probably null
R8054:Akap9	UTSW	5	4,038,707 (GRCm38)	critical splice donor site	probably null
R8116:Akap9	UTSW	5	4,061,183 (GRCm38)	missense	probably benign	0.04
R8150:Akap9	UTSW	5	3,961,982 (GRCm38)	missense	probably damaging	1.00
R8234:Akap9	UTSW	5	4,044,845 (GRCm38)	missense	probably benign	0.18
R8348:Akap9	UTSW	5	3,948,897 (GRCm38)	critical splice donor site	probably null
R8365:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R8366:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R8448:Akap9	UTSW	5	3,948,897 (GRCm38)	critical splice donor site	probably null
R8466:Akap9	UTSW	5	4,038,659 (GRCm38)	missense	probably damaging	1.00
R8772:Akap9	UTSW	5	4,046,255 (GRCm38)	missense	probably damaging	1.00
R8881:Akap9	UTSW	5	3,961,279 (GRCm38)	missense
R8937:Akap9	UTSW	5	4,044,048 (GRCm38)	missense	possibly damaging	0.78
R8956:Akap9	UTSW	5	3,948,805 (GRCm38)	missense	possibly damaging	0.79
R9000:Akap9	UTSW	5	4,055,650 (GRCm38)	missense	probably benign
R9049:Akap9	UTSW	5	4,064,597 (GRCm38)	missense
R9074:Akap9	UTSW	5	4,077,959 (GRCm38)	missense	probably benign	0.40
R9124:Akap9	UTSW	5	4,061,284 (GRCm38)	missense	probably damaging	0.99
R9129:Akap9	UTSW	5	4,069,089 (GRCm38)	missense	probably benign	0.00
R9371:Akap9	UTSW	5	3,961,852 (GRCm38)	missense	possibly damaging	0.83
R9424:Akap9	UTSW	5	3,962,224 (GRCm38)	nonsense	probably null
R9424:Akap9	UTSW	5	3,962,223 (GRCm38)	nonsense	probably null
R9509:Akap9	UTSW	5	4,046,349 (GRCm38)	missense	probably benign
R9515:Akap9	UTSW	5	4,055,709 (GRCm38)	missense	probably damaging	1.00
R9567:Akap9	UTSW	5	4,077,311 (GRCm38)	missense	possibly damaging	0.89
R9587:Akap9	UTSW	5	4,069,149 (GRCm38)	missense	probably damaging	1.00
R9619:Akap9	UTSW	5	4,044,833 (GRCm38)	missense	probably damaging	1.00
R9635:Akap9	UTSW	5	4,050,545 (GRCm38)	missense	probably benign	0.20
R9680:Akap9	UTSW	5	3,961,587 (GRCm38)	missense	probably benign	0.03
R9691:Akap9	UTSW	5	3,960,491 (GRCm38)	missense	probably damaging	1.00
R9726:Akap9	UTSW	5	4,003,757 (GRCm38)	missense	probably benign	0.39
U15987:Akap9	UTSW	5	4,067,924 (GRCm38)	critical splice donor site	probably null
X0026:Akap9	UTSW	5	4,014,039 (GRCm38)	missense	probably damaging	1.00
X0057:Akap9	UTSW	5	3,975,598 (GRCm38)	critical splice acceptor site	probably null
Z1176:Akap9	UTSW	5	3,962,251 (GRCm38)	missense	probably damaging	0.96
Z1177:Akap9	UTSW	5	4,046,189 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTCACGTTGGAGCTCATGTTTAAC -3'
(R):5'- AGCTCTCCTCATCTTCTGGGAG -3'

Sequencing Primer
(F):5'- GGAGCTCATGTTTAACAAAATCCCAG -3'
(R):5'- CTCATCTTCTGGGAGGGTCC -3'

Posted On

2019-09-13