Mutagenetix > Incidental Mutation

Incidental Mutation 'R8289:Prune2'

638608

Institutional Source

Beutler Lab

Gene Symbol

Prune2

Ensembl Gene

ENSMUSG00000039126

Gene Name

prune homolog 2

Synonyms

A230083H22Rik, A330102H22Rik, 6330414G02Rik, Olfaxin

MMRRC Submission

067711-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8289 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

16933482-17201296 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 17100373 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 1959 (H1959L)

Ref Sequence

ENSEMBL: ENSMUSP00000084977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087689]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000087689
AA Change: H1959L

PolyPhen 2 Score 0.434 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000084977
Gene: ENSMUSG00000039126
AA Change: H1959L

Domain	Start	End	E-Value	Type
DHHA2	208	351	8.32e-17	SMART
low complexity region	433	445	N/A	INTRINSIC
low complexity region	476	488	N/A	INTRINSIC
low complexity region	547	553	N/A	INTRINSIC
low complexity region	962	975	N/A	INTRINSIC
low complexity region	1071	1082	N/A	INTRINSIC
low complexity region	1368	1378	N/A	INTRINSIC
low complexity region	1533	1545	N/A	INTRINSIC
low complexity region	1668	1685	N/A	INTRINSIC
low complexity region	1740	1751	N/A	INTRINSIC
low complexity region	2162	2175	N/A	INTRINSIC
low complexity region	2222	2233	N/A	INTRINSIC
low complexity region	2591	2606	N/A	INTRINSIC
low complexity region	2731	2744	N/A	INTRINSIC
SEC14	2882	3037	2.08e-12	SMART

Meta Mutation Damage Score

0.1044

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (62/62)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. Prostate cancer antigen 3 regulates levels of this gene through formation of a double-stranded RNA that undergoes adenosine deaminase actin on RNA-dependent adenosine-to-inosine RNA editing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Allele List at MGI

All alleles(160) : Gene trapped(160)

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	G	C	11: 109,927,515 (GRCm39)		probably benign	Het
Adamts3	C	A	5: 89,923,282 (GRCm39)	D175Y	possibly damaging	Het
Adra1b	G	A	11: 43,726,315 (GRCm39)	P201S	probably damaging	Het
Ahnak2	A	G	12: 112,742,242 (GRCm39)	V610A	possibly damaging	Het
Ap5s1	A	C	2: 131,054,379 (GRCm39)	H62P	probably benign	Het
Apeh	A	G	9: 107,963,444 (GRCm39)	S593P	probably damaging	Het
Arhgap24	T	C	5: 103,028,692 (GRCm39)	V289A	possibly damaging	Het
Atoh1	A	G	6: 64,706,893 (GRCm39)	E196G	probably damaging	Het
Atp1b3	G	A	9: 96,215,602 (GRCm39)	S271L	probably benign	Het
Atp6v0a1	A	G	11: 100,924,931 (GRCm39)	R339G	probably damaging	Het
Calb2	T	C	8: 110,894,690 (GRCm39)	H27R	possibly damaging	Het
Camkk2	A	C	5: 122,894,689 (GRCm39)	L201R	probably damaging	Het
Car15	A	G	16: 17,654,580 (GRCm39)		probably null	Het
Cecr2	A	G	6: 120,735,077 (GRCm39)	N771S	probably benign	Het
Celsr1	G	T	15: 85,917,286 (GRCm39)	S229*	probably null	Het
Csmd1	T	A	8: 16,108,716 (GRCm39)	I1896F	probably damaging	Het
Cubn	C	T	2: 13,491,613 (GRCm39)	V145I	probably benign	Het
Cuedc1	G	A	11: 88,073,381 (GRCm39)	S182N	probably benign	Het
Cux1	T	A	5: 136,337,358 (GRCm39)	E718V	probably damaging	Het
Cyp7a1	G	A	4: 6,268,295 (GRCm39)	P477S	probably damaging	Het
Dcaf17	T	A	2: 70,885,718 (GRCm39)	W16R		Het
Dhx8	T	A	11: 101,631,571 (GRCm39)	M388K	probably benign	Het
Dnah14	T	A	1: 181,543,780 (GRCm39)	Y2453*	probably null	Het
Epha8	G	T	4: 136,665,897 (GRCm39)	L420M	probably damaging	Het
F830016B08Rik	G	A	18: 60,433,360 (GRCm39)	E148K	probably benign	Het
Hectd4	A	G	5: 121,404,424 (GRCm39)	T434A	possibly damaging	Het
Ide	A	G	19: 37,290,952 (GRCm39)	L368P		Het
Ide	G	C	19: 37,290,953 (GRCm39)	L368V	probably null	Het
Ifi206	T	C	1: 173,308,032 (GRCm39)	I655V		Het
Insrr	A	G	3: 87,721,501 (GRCm39)	M1136V	probably damaging	Het
Kcne2	A	G	16: 92,093,707 (GRCm39)	E78G	probably damaging	Het
Lrrc23	A	T	6: 124,755,267 (GRCm39)	L90H	probably damaging	Het
Magel2	A	C	7: 62,028,875 (GRCm39)	Q593P	unknown	Het
Mrgpra3	G	A	7: 47,239,468 (GRCm39)	H153Y	possibly damaging	Het
Msln	A	G	17: 25,967,880 (GRCm39)	V595A	possibly damaging	Het
Mvb12a	T	C	8: 71,995,703 (GRCm39)		probably null	Het
Myo7a	T	G	7: 97,726,376 (GRCm39)	M966L	probably benign	Het
Ncoa5	C	T	2: 164,854,982 (GRCm39)	D47N	possibly damaging	Het
Neurl4	T	A	11: 69,800,206 (GRCm39)		probably null	Het
Or1e29	T	A	11: 73,667,839 (GRCm39)	M105L	probably benign	Het
Or4d1	T	C	11: 87,805,589 (GRCm39)	T48A	probably benign	Het
Or5p75-ps1	T	A	7: 108,108,125 (GRCm39)	H287Q	unknown	Het
Or8b37	T	A	9: 37,959,000 (GRCm39)	C161S	probably benign	Het
Osbpl7	T	A	11: 96,947,405 (GRCm39)	S480T	probably benign	Het
Podnl1	T	C	8: 84,858,552 (GRCm39)	L484P		Het
Polr3gl	G	T	3: 96,489,228 (GRCm39)		probably benign	Het
Prkaa1	G	T	15: 5,206,563 (GRCm39)	V438L	possibly damaging	Het
Rad50	A	T	11: 53,589,685 (GRCm39)	L172*	probably null	Het
Samsn1	C	A	16: 75,685,684 (GRCm39)	G25W	probably damaging	Het
Sbno1	T	G	5: 124,542,068 (GRCm39)	L362F	probably damaging	Het
Slc26a6	G	A	9: 108,733,230 (GRCm39)	R43H	probably benign	Het
Slc35f5	T	G	1: 125,490,252 (GRCm39)	Y49*	probably null	Het
Slc41a2	T	C	10: 83,137,044 (GRCm39)	I288V	probably benign	Het
Slc9c1	A	G	16: 45,403,344 (GRCm39)	M804V	probably benign	Het
Smc6	T	A	12: 11,324,052 (GRCm39)	S66T	probably benign	Het
Ssh1	T	C	5: 114,080,445 (GRCm39)	D995G	probably benign	Het
Stk11	G	T	10: 79,961,740 (GRCm39)		probably benign	Het
Sycp1	A	G	3: 102,748,353 (GRCm39)	I813T	probably benign	Het
Tmem121b	A	T	6: 120,469,722 (GRCm39)	S332T	possibly damaging	Het
Treml4	A	C	17: 48,581,456 (GRCm39)	I244L	probably benign	Het
Unc13b	T	A	4: 43,172,524 (GRCm39)	C1117*	probably null	Het
Wdr35	A	G	12: 9,058,020 (GRCm39)	I526V	probably benign	Het
Zap70	T	C	1: 36,820,218 (GRCm39)	W500R	probably damaging	Het
Zfr	G	T	15: 12,135,357 (GRCm39)	V49F	noncoding transcript	Het

Other mutations in Prune2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00234:Prune2	APN	19	17,145,708 (GRCm39)	critical splice donor site	probably null
IGL00848:Prune2	APN	19	17,096,482 (GRCm39)	missense	probably damaging	1.00
IGL00862:Prune2	APN	19	17,096,713 (GRCm39)	missense	probably benign	0.41
IGL00915:Prune2	APN	19	16,993,617 (GRCm39)	missense	probably damaging	1.00
IGL01084:Prune2	APN	19	17,095,573 (GRCm39)	missense	probably benign	0.19
IGL01109:Prune2	APN	19	17,101,243 (GRCm39)	missense	probably benign	0.03
IGL01372:Prune2	APN	19	17,102,433 (GRCm39)	missense	probably damaging	1.00
IGL01650:Prune2	APN	19	17,145,656 (GRCm39)	missense	possibly damaging	0.95
IGL01752:Prune2	APN	19	17,101,267 (GRCm39)	missense	possibly damaging	0.50
IGL01812:Prune2	APN	19	16,981,141 (GRCm39)	missense	possibly damaging	0.50
IGL01902:Prune2	APN	19	17,096,002 (GRCm39)	missense	probably benign	0.00
IGL02195:Prune2	APN	19	17,096,921 (GRCm39)	missense	probably benign	0.00
IGL02502:Prune2	APN	19	17,101,245 (GRCm39)	missense	probably benign	0.00
IGL02569:Prune2	APN	19	17,156,223 (GRCm39)	missense	probably damaging	0.99
IGL02693:Prune2	APN	19	17,101,855 (GRCm39)	missense	probably benign	0.03
IGL02737:Prune2	APN	19	17,170,775 (GRCm39)	nonsense	probably null
IGL02794:Prune2	APN	19	17,096,725 (GRCm39)	missense	probably benign	0.19
IGL02985:Prune2	APN	19	16,993,723 (GRCm39)	critical splice donor site	probably null
IGL03349:Prune2	APN	19	17,100,710 (GRCm39)	missense	probably damaging	1.00
3-1:Prune2	UTSW	19	17,102,646 (GRCm39)	missense	probably benign	0.00
R0060:Prune2	UTSW	19	16,981,097 (GRCm39)	missense	probably damaging	1.00
R0098:Prune2	UTSW	19	17,101,267 (GRCm39)	missense	possibly damaging	0.50
R0098:Prune2	UTSW	19	17,101,267 (GRCm39)	missense	possibly damaging	0.50
R0165:Prune2	UTSW	19	17,099,974 (GRCm39)	missense	probably benign	0.00
R0277:Prune2	UTSW	19	17,098,753 (GRCm39)	missense	probably damaging	0.99
R0321:Prune2	UTSW	19	17,099,818 (GRCm39)	missense	probably benign	0.39
R0321:Prune2	UTSW	19	17,098,291 (GRCm39)	missense	possibly damaging	0.78
R0374:Prune2	UTSW	19	17,098,274 (GRCm39)	missense	probably benign	0.00
R0380:Prune2	UTSW	19	17,101,371 (GRCm39)	missense	probably damaging	1.00
R0396:Prune2	UTSW	19	17,100,444 (GRCm39)	missense	probably benign	0.35
R0408:Prune2	UTSW	19	17,099,674 (GRCm39)	missense	probably benign	0.00
R0421:Prune2	UTSW	19	17,100,675 (GRCm39)	missense	probably benign	0.02
R0480:Prune2	UTSW	19	16,984,156 (GRCm39)	splice site	probably benign
R0531:Prune2	UTSW	19	16,984,117 (GRCm39)	missense	probably damaging	1.00
R0546:Prune2	UTSW	19	16,998,030 (GRCm39)	splice site	probably benign
R0554:Prune2	UTSW	19	17,102,582 (GRCm39)	nonsense	probably null
R0659:Prune2	UTSW	19	17,100,199 (GRCm39)	missense	probably damaging	1.00
R0699:Prune2	UTSW	19	17,101,319 (GRCm39)	missense	probably damaging	1.00
R0781:Prune2	UTSW	19	17,102,586 (GRCm39)	missense	probably benign
R1110:Prune2	UTSW	19	17,102,586 (GRCm39)	missense	probably benign
R1178:Prune2	UTSW	19	17,100,469 (GRCm39)	missense	probably benign	0.22
R1181:Prune2	UTSW	19	17,100,469 (GRCm39)	missense	probably benign	0.22
R1337:Prune2	UTSW	19	17,096,971 (GRCm39)	missense	possibly damaging	0.70
R1356:Prune2	UTSW	19	17,189,681 (GRCm39)	missense	probably benign	0.40
R1385:Prune2	UTSW	19	17,102,312 (GRCm39)	missense	possibly damaging	0.50
R1659:Prune2	UTSW	19	17,098,015 (GRCm39)	missense	possibly damaging	0.59
R1738:Prune2	UTSW	19	17,102,374 (GRCm39)	missense	probably benign	0.01
R1756:Prune2	UTSW	19	17,101,068 (GRCm39)	missense	probably benign	0.01
R1765:Prune2	UTSW	19	17,102,962 (GRCm39)	missense	probably damaging	1.00
R1782:Prune2	UTSW	19	17,099,537 (GRCm39)	missense	probably benign	0.00
R1817:Prune2	UTSW	19	17,099,445 (GRCm39)	missense	probably benign	0.00
R1838:Prune2	UTSW	19	17,177,242 (GRCm39)	missense	probably damaging	1.00
R1851:Prune2	UTSW	19	17,176,503 (GRCm39)	missense	probably damaging	1.00
R1852:Prune2	UTSW	19	17,176,503 (GRCm39)	missense	probably damaging	1.00
R1866:Prune2	UTSW	19	17,100,856 (GRCm39)	missense	probably damaging	1.00
R1911:Prune2	UTSW	19	17,091,038 (GRCm39)	missense	probably benign	0.02
R1983:Prune2	UTSW	19	16,998,006 (GRCm39)	missense	probably damaging	0.97
R2014:Prune2	UTSW	19	17,097,887 (GRCm39)	missense	probably damaging	1.00
R2066:Prune2	UTSW	19	17,098,042 (GRCm39)	missense	possibly damaging	0.57
R2088:Prune2	UTSW	19	17,097,109 (GRCm39)	missense	possibly damaging	0.95
R2111:Prune2	UTSW	19	17,185,602 (GRCm39)	missense	probably damaging	1.00
R2128:Prune2	UTSW	19	17,099,786 (GRCm39)	missense	probably benign	0.00
R2165:Prune2	UTSW	19	17,097,546 (GRCm39)	missense	probably benign	0.19
R2241:Prune2	UTSW	19	17,100,456 (GRCm39)	missense	probably damaging	0.96
R2278:Prune2	UTSW	19	17,095,919 (GRCm39)	missense	possibly damaging	0.93
R2504:Prune2	UTSW	19	16,977,400 (GRCm39)	missense	probably damaging	1.00
R2508:Prune2	UTSW	19	17,099,986 (GRCm39)	missense	probably benign	0.43
R3055:Prune2	UTSW	19	17,102,407 (GRCm39)	missense	probably damaging	0.98
R3086:Prune2	UTSW	19	17,098,777 (GRCm39)	missense	possibly damaging	0.75
R3104:Prune2	UTSW	19	17,096,520 (GRCm39)	missense	probably damaging	1.00
R3105:Prune2	UTSW	19	17,096,520 (GRCm39)	missense	probably damaging	1.00
R3547:Prune2	UTSW	19	17,101,712 (GRCm39)	missense	probably damaging	0.96
R3702:Prune2	UTSW	19	17,156,235 (GRCm39)	missense	probably damaging	1.00
R3753:Prune2	UTSW	19	17,102,818 (GRCm39)	missense	probably benign	0.38
R3933:Prune2	UTSW	19	17,101,318 (GRCm39)	missense	probably damaging	1.00
R3935:Prune2	UTSW	19	17,177,150 (GRCm39)	missense	probably damaging	1.00
R4022:Prune2	UTSW	19	16,977,384 (GRCm39)	missense	probably damaging	1.00
R4042:Prune2	UTSW	19	16,981,190 (GRCm39)	critical splice donor site	probably null
R4164:Prune2	UTSW	19	16,981,098 (GRCm39)	missense	possibly damaging	0.87
R4453:Prune2	UTSW	19	17,099,274 (GRCm39)	missense	probably benign	0.00
R4642:Prune2	UTSW	19	16,998,019 (GRCm39)	critical splice donor site	probably null
R4661:Prune2	UTSW	19	16,977,387 (GRCm39)	missense	probably damaging	1.00
R4666:Prune2	UTSW	19	17,097,552 (GRCm39)	nonsense	probably null
R4823:Prune2	UTSW	19	17,097,868 (GRCm39)	missense	probably damaging	1.00
R4897:Prune2	UTSW	19	17,099,219 (GRCm39)	missense	probably benign	0.03
R4922:Prune2	UTSW	19	17,100,116 (GRCm39)	missense	probably benign	0.00
R4962:Prune2	UTSW	19	17,099,637 (GRCm39)	missense	probably benign	0.11
R5026:Prune2	UTSW	19	17,176,506 (GRCm39)	missense	probably damaging	1.00
R5042:Prune2	UTSW	19	17,097,161 (GRCm39)	missense	possibly damaging	0.94
R5124:Prune2	UTSW	19	17,177,274 (GRCm39)	missense	probably damaging	1.00
R5133:Prune2	UTSW	19	16,980,995 (GRCm39)	missense	probably damaging	1.00
R5184:Prune2	UTSW	19	17,193,721 (GRCm39)	missense	possibly damaging	0.95
R5234:Prune2	UTSW	19	17,096,032 (GRCm39)	missense	probably damaging	1.00
R5339:Prune2	UTSW	19	17,098,236 (GRCm39)	missense	probably damaging	1.00
R5363:Prune2	UTSW	19	17,095,630 (GRCm39)	missense	probably damaging	1.00
R5382:Prune2	UTSW	19	16,981,023 (GRCm39)	missense	probably damaging	1.00
R5436:Prune2	UTSW	19	16,998,007 (GRCm39)	missense	probably damaging	1.00
R5480:Prune2	UTSW	19	17,098,311 (GRCm39)	missense	possibly damaging	0.66
R5635:Prune2	UTSW	19	17,095,573 (GRCm39)	missense	probably benign	0.19
R5678:Prune2	UTSW	19	17,096,032 (GRCm39)	missense	probably damaging	1.00
R5814:Prune2	UTSW	19	16,993,725 (GRCm39)	splice site	probably null
R5894:Prune2	UTSW	19	17,098,755 (GRCm39)	missense	possibly damaging	0.88
R6011:Prune2	UTSW	19	17,096,080 (GRCm39)	missense	probably benign	0.35
R6207:Prune2	UTSW	19	17,095,480 (GRCm39)	missense	probably damaging	1.00
R6218:Prune2	UTSW	19	17,098,926 (GRCm39)	missense	probably benign	0.00
R6573:Prune2	UTSW	19	17,098,521 (GRCm39)	missense	probably damaging	1.00
R6573:Prune2	UTSW	19	17,098,522 (GRCm39)	missense	possibly damaging	0.61
R6734:Prune2	UTSW	19	16,981,097 (GRCm39)	missense	probably damaging	1.00
R6805:Prune2	UTSW	19	17,097,954 (GRCm39)	missense	probably benign
R6837:Prune2	UTSW	19	17,156,292 (GRCm39)	missense	probably damaging	1.00
R6850:Prune2	UTSW	19	17,099,552 (GRCm39)	missense	probably benign	0.00
R6858:Prune2	UTSW	19	17,095,470 (GRCm39)	missense	possibly damaging	0.70
R6874:Prune2	UTSW	19	17,100,592 (GRCm39)	missense	probably damaging	1.00
R6954:Prune2	UTSW	19	16,977,385 (GRCm39)	missense	probably damaging	1.00
R7098:Prune2	UTSW	19	17,097,966 (GRCm39)	missense	probably benign	0.39
R7102:Prune2	UTSW	19	17,098,577 (GRCm39)	missense	probably benign	0.24
R7246:Prune2	UTSW	19	17,098,732 (GRCm39)	missense	probably damaging	0.99
R7284:Prune2	UTSW	19	17,097,250 (GRCm39)	missense	probably damaging	1.00
R7295:Prune2	UTSW	19	17,097,261 (GRCm39)	missense	probably benign	0.01
R7371:Prune2	UTSW	19	17,096,734 (GRCm39)	missense	probably benign	0.02
R7651:Prune2	UTSW	19	17,097,772 (GRCm39)	missense	probably damaging	1.00
R7830:Prune2	UTSW	19	17,100,038 (GRCm39)	missense	probably benign	0.21
R7872:Prune2	UTSW	19	17,096,798 (GRCm39)	missense	probably benign	0.05
R7881:Prune2	UTSW	19	17,100,393 (GRCm39)	missense	possibly damaging	0.50
R7966:Prune2	UTSW	19	17,156,223 (GRCm39)	missense	probably damaging	0.99
R7969:Prune2	UTSW	19	17,179,034 (GRCm39)	missense	probably damaging	0.98
R8092:Prune2	UTSW	19	17,097,357 (GRCm39)	missense	probably damaging	1.00
R8110:Prune2	UTSW	19	17,098,083 (GRCm39)	missense	probably benign	0.22
R8115:Prune2	UTSW	19	17,101,288 (GRCm39)	missense	probably benign	0.02
R8129:Prune2	UTSW	19	17,096,200 (GRCm39)	missense	probably benign	0.01
R8169:Prune2	UTSW	19	17,102,455 (GRCm39)	missense	probably benign	0.10
R8171:Prune2	UTSW	19	17,097,882 (GRCm39)	missense	probably damaging	1.00
R8176:Prune2	UTSW	19	17,095,656 (GRCm39)	missense	probably damaging	1.00
R8200:Prune2	UTSW	19	17,102,337 (GRCm39)	missense	probably benign	0.01
R8217:Prune2	UTSW	19	17,097,480 (GRCm39)	missense	probably benign	0.01
R8258:Prune2	UTSW	19	17,189,672 (GRCm39)	missense	unknown
R8259:Prune2	UTSW	19	17,189,672 (GRCm39)	missense	unknown
R8329:Prune2	UTSW	19	17,098,629 (GRCm39)	missense	probably benign	0.02
R8342:Prune2	UTSW	19	17,103,027 (GRCm39)	missense	probably benign	0.01
R8558:Prune2	UTSW	19	17,099,602 (GRCm39)	missense	probably damaging	0.98
R8732:Prune2	UTSW	19	17,097,769 (GRCm39)	missense	probably damaging	1.00
R8743:Prune2	UTSW	19	17,096,920 (GRCm39)	missense	probably benign	0.22
R8769:Prune2	UTSW	19	17,100,442 (GRCm39)	missense	probably damaging	0.96
R8862:Prune2	UTSW	19	17,097,510 (GRCm39)	missense	probably benign	0.04
R8936:Prune2	UTSW	19	17,099,199 (GRCm39)	missense	probably benign	0.24
R9040:Prune2	UTSW	19	17,097,991 (GRCm39)	missense	probably damaging	1.00
R9084:Prune2	UTSW	19	17,097,741 (GRCm39)	missense	probably damaging	1.00
R9224:Prune2	UTSW	19	17,097,393 (GRCm39)	missense	probably damaging	1.00
R9273:Prune2	UTSW	19	17,095,690 (GRCm39)	missense	possibly damaging	0.74
R9275:Prune2	UTSW	19	17,101,144 (GRCm39)	missense	probably benign	0.06
R9278:Prune2	UTSW	19	17,101,144 (GRCm39)	missense	probably benign	0.06
R9290:Prune2	UTSW	19	17,145,691 (GRCm39)	missense	probably benign	0.41
R9305:Prune2	UTSW	19	17,097,625 (GRCm39)	missense	probably benign	0.14
R9317:Prune2	UTSW	19	17,099,034 (GRCm39)	missense	probably benign	0.00
R9354:Prune2	UTSW	19	17,099,986 (GRCm39)	missense	probably benign	0.43
R9373:Prune2	UTSW	19	17,099,502 (GRCm39)	missense	probably benign
R9394:Prune2	UTSW	19	16,981,053 (GRCm39)	missense	probably damaging	1.00
R9405:Prune2	UTSW	19	17,193,708 (GRCm39)	missense	probably damaging	0.99
R9476:Prune2	UTSW	19	17,096,706 (GRCm39)	missense	possibly damaging	0.64
R9532:Prune2	UTSW	19	17,099,794 (GRCm39)	missense	probably benign	0.00
X0019:Prune2	UTSW	19	17,098,881 (GRCm39)	missense	probably benign	0.16
X0028:Prune2	UTSW	19	17,100,249 (GRCm39)	missense	probably damaging	1.00
X0064:Prune2	UTSW	19	17,099,739 (GRCm39)	missense	probably damaging	1.00
X0066:Prune2	UTSW	19	17,096,154 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTCACAGGAGAAACTTTGGAAC -3'
(R):5'- TCCAATCAGAGTCCAGCTTCC -3'

Sequencing Primer
(F):5'- AGCTGGATTCAGATGCCAATC -3'
(R):5'- AGAGTCCAGCTTCCTCATCAG -3'

Posted On

2020-07-28