Mutagenetix > Incidental Mutation

Incidental Mutation 'R8518:Tns3'

656259

Institutional Source

Beutler Lab

Gene Symbol

Tns3

Ensembl Gene

ENSMUSG00000020422

Gene Name

tensin 3

Synonyms

F830010I22Rik, Tens1, TEM6

MMRRC Submission

067850-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.333) question?

Stock #

R8518 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

8381652-8614681 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 8442971 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 464 (V464E)

Ref Sequence

ENSEMBL: ENSMUSP00000020695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020695]

AlphaFold

Q5SSZ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000020695
AA Change: V464E

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000020695
Gene: ENSMUSG00000020422
AA Change: V464E

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	1	171	5e-28	SMART
PTEN_C2	173	300	1.15e-48	SMART
low complexity region	854	864	N/A	INTRINSIC
low complexity region	1102	1126	N/A	INTRINSIC
SH2	1165	1268	1.32e-18	SMART
PTB	1301	1438	3.14e-24	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit one third postnatal lethality, reduced body weight, growth retardation, smaller digestive tracts with defects in villi and enterocyte differentiation, abnormal lung morphology, and thinner bones with decreased chondrocyte proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc5	A	G	16: 20,223,398 (GRCm39)	V170A	possibly damaging	Het
Adamts2	A	G	11: 50,666,957 (GRCm39)	E418G	probably damaging	Het
Adra2b	T	C	2: 127,206,056 (GRCm39)	L191P	probably damaging	Het
Ampd2	C	A	3: 107,987,432 (GRCm39)	V134L	probably benign	Het
Bag2	T	C	1: 33,787,358 (GRCm39)	I55V	probably benign	Het
Bltp2	T	C	11: 78,156,064 (GRCm39)	L192P	possibly damaging	Het
Bmpr1b	T	C	3: 141,563,343 (GRCm39)	I200M	possibly damaging	Het
Cacna1i	C	A	15: 80,243,095 (GRCm39)	Y417*	probably null	Het
Cdc7	T	A	5: 107,120,864 (GRCm39)	I162N	probably damaging	Het
Chchd1	T	C	14: 20,754,211 (GRCm39)	I89T	probably benign	Het
Cxcl16	T	C	11: 70,346,510 (GRCm39)	N224S	probably benign	Het
Dync1i1	T	G	6: 5,913,330 (GRCm39)	L188V	probably damaging	Het
Dync2h1	A	G	9: 7,051,452 (GRCm39)	V3161A	probably benign	Het
E2f3	T	A	13: 30,097,453 (GRCm39)	K303N	probably damaging	Het
Fermt1	T	C	2: 132,748,635 (GRCm39)	T663A	probably benign	Het
Fhod3	A	G	18: 25,189,390 (GRCm39)	R611G	probably damaging	Het
Frem3	A	T	8: 81,339,224 (GRCm39)	S506C	probably damaging	Het
Gm10318	T	A	10: 77,688,712 (GRCm39)	M6K	unknown	Het
Gm6563	T	A	19: 23,653,480 (GRCm39)	V90E	probably damaging	Het
Gpc6	T	C	14: 117,163,384 (GRCm39)	S13P	probably benign	Het
Gpr107	C	A	2: 31,066,939 (GRCm39)	H196Q	probably benign	Het
Heatr1	T	G	13: 12,425,415 (GRCm39)	D618E	probably benign	Het
Lrrc27	T	C	7: 138,808,690 (GRCm39)	V391A	probably benign	Het
Mindy3	T	G	2: 12,360,154 (GRCm39)	K349N	probably damaging	Het
Mllt10	T	C	2: 18,151,670 (GRCm39)	V261A	probably damaging	Het
Mrpl48	G	A	7: 100,232,269 (GRCm39)		probably benign	Het
Msantd5f5	T	A	4: 73,542,641 (GRCm39)	L47Q	probably damaging	Het
Muc16	T	C	9: 18,430,927 (GRCm39)	I8062V	probably benign	Het
Myo7a	T	C	7: 97,740,270 (GRCm39)	N575D	possibly damaging	Het
Nfrkb	T	C	9: 31,311,261 (GRCm39)	L370P	probably damaging	Het
Numbl	T	C	7: 26,964,361 (GRCm39)	V57A	probably benign	Het
Or51t4	T	C	7: 102,597,804 (GRCm39)	V44A	probably damaging	Het
Or5b12	G	A	19: 12,896,959 (GRCm39)	T238I	probably damaging	Het
Or6y1	T	A	1: 174,276,742 (GRCm39)	C184*	probably null	Het
Pdia2	A	T	17: 26,417,144 (GRCm39)	L100*	probably null	Het
Prpf31	A	T	7: 3,635,742 (GRCm39)	N105I	probably damaging	Het
Rbm20	A	G	19: 53,839,923 (GRCm39)	N971D	probably benign	Het
Rnf213	T	A	11: 119,353,043 (GRCm39)	M3980K		Het
Skint6	A	G	4: 113,095,465 (GRCm39)	F65L	possibly damaging	Het
Smpd4	T	A	16: 17,458,884 (GRCm39)	C536S	possibly damaging	Het
Tcof1	G	C	18: 60,962,123 (GRCm39)	A702G	possibly damaging	Het
Tfpt	A	G	7: 3,632,065 (GRCm39)	F15S	possibly damaging	Het
Tnp2	A	G	16: 10,606,372 (GRCm39)	C32R	possibly damaging	Het
Tpp2	T	A	1: 44,019,545 (GRCm39)	L824H	probably damaging	Het
Trav15-1-dv6-1	A	G	14: 53,797,666 (GRCm39)	D105G	probably benign	Het
Ttc3	T	C	16: 94,258,238 (GRCm39)	S1679P	probably benign	Het
Xpo7	A	G	14: 70,944,837 (GRCm39)	Y19H	probably damaging	Het
Zswim4	G	A	8: 84,938,586 (GRCm39)	R1099W	probably damaging	Het

Other mutations in Tns3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Tns3	APN	11	8,401,066 (GRCm39)	missense	probably benign	0.42
IGL00822:Tns3	APN	11	8,393,976 (GRCm39)	missense	probably damaging	0.99
IGL01075:Tns3	APN	11	8,428,399 (GRCm39)	missense	probably benign	0.45
IGL01286:Tns3	APN	11	8,442,617 (GRCm39)	missense	probably benign	0.01
IGL01680:Tns3	APN	11	8,498,937 (GRCm39)	missense	probably damaging	1.00
IGL01687:Tns3	APN	11	8,442,798 (GRCm39)	missense	probably damaging	1.00
IGL01734:Tns3	APN	11	8,469,192 (GRCm39)	splice site	probably benign
IGL01844:Tns3	APN	11	8,387,177 (GRCm39)	missense	possibly damaging	0.58
IGL01984:Tns3	APN	11	8,498,992 (GRCm39)	nonsense	probably null
IGL02137:Tns3	APN	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
IGL02273:Tns3	APN	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
IGL02623:Tns3	APN	11	8,387,141 (GRCm39)	missense	probably damaging	1.00
IGL02697:Tns3	APN	11	8,442,346 (GRCm39)	missense	probably benign	0.00
IGL02829:Tns3	APN	11	8,469,564 (GRCm39)	missense	probably damaging	1.00
ANU74:Tns3	UTSW	11	8,442,149 (GRCm39)	missense	probably benign	0.38
R0020:Tns3	UTSW	11	8,495,227 (GRCm39)	critical splice donor site	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0370:Tns3	UTSW	11	8,395,730 (GRCm39)	missense	possibly damaging	0.80
R0388:Tns3	UTSW	11	8,395,703 (GRCm39)	missense	probably benign	0.07
R0410:Tns3	UTSW	11	8,385,852 (GRCm39)	missense	probably benign	0.02
R0496:Tns3	UTSW	11	8,497,262 (GRCm39)	splice site	probably benign
R0562:Tns3	UTSW	11	8,443,262 (GRCm39)	missense	possibly damaging	0.93
R0626:Tns3	UTSW	11	8,443,121 (GRCm39)	missense	probably benign	0.04
R0736:Tns3	UTSW	11	8,469,474 (GRCm39)	missense	possibly damaging	0.94
R0893:Tns3	UTSW	11	8,443,302 (GRCm39)	missense	probably damaging	1.00
R1367:Tns3	UTSW	11	8,398,704 (GRCm39)	missense	probably benign	0.01
R1386:Tns3	UTSW	11	8,468,261 (GRCm39)	missense	probably benign	0.02
R1975:Tns3	UTSW	11	8,385,738 (GRCm39)	missense	probably benign	0.04
R2205:Tns3	UTSW	11	8,481,719 (GRCm39)	missense	probably damaging	1.00
R2319:Tns3	UTSW	11	8,491,200 (GRCm39)	missense	probably damaging	1.00
R2830:Tns3	UTSW	11	8,385,870 (GRCm39)	missense	probably damaging	1.00
R3720:Tns3	UTSW	11	8,442,999 (GRCm39)	missense	probably damaging	1.00
R3765:Tns3	UTSW	11	8,401,133 (GRCm39)	missense	probably benign	0.00
R3817:Tns3	UTSW	11	8,384,619 (GRCm39)	missense	probably damaging	1.00
R4058:Tns3	UTSW	11	8,442,275 (GRCm39)	missense	probably damaging	1.00
R4599:Tns3	UTSW	11	8,481,747 (GRCm39)	missense	probably damaging	1.00
R4631:Tns3	UTSW	11	8,401,119 (GRCm39)	missense	probably benign	0.30
R4731:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4732:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4733:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R5472:Tns3	UTSW	11	8,401,092 (GRCm39)	missense	probably benign
R5749:Tns3	UTSW	11	8,401,177 (GRCm39)	missense	probably benign	0.01
R5807:Tns3	UTSW	11	8,443,211 (GRCm39)	missense	probably damaging	1.00
R5844:Tns3	UTSW	11	8,384,580 (GRCm39)	missense	probably damaging	1.00
R5942:Tns3	UTSW	11	8,385,860 (GRCm39)	missense	probably damaging	1.00
R5982:Tns3	UTSW	11	8,442,245 (GRCm39)	missense	probably damaging	0.99
R6025:Tns3	UTSW	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
R6266:Tns3	UTSW	11	8,442,987 (GRCm39)	missense	probably damaging	1.00
R6322:Tns3	UTSW	11	8,442,147 (GRCm39)	missense	probably benign	0.01
R6536:Tns3	UTSW	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,058 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,057 (GRCm39)	missense	probably damaging	1.00
R6864:Tns3	UTSW	11	8,443,196 (GRCm39)	missense	probably damaging	1.00
R6897:Tns3	UTSW	11	8,481,743 (GRCm39)	missense	probably damaging	1.00
R7108:Tns3	UTSW	11	8,387,251 (GRCm39)	missense	probably benign	0.00
R7443:Tns3	UTSW	11	8,401,442 (GRCm39)	missense	probably benign	0.01
R7459:Tns3	UTSW	11	8,442,793 (GRCm39)	missense	probably benign	0.16
R7474:Tns3	UTSW	11	8,480,894 (GRCm39)	missense	probably damaging	1.00
R7576:Tns3	UTSW	11	8,491,192 (GRCm39)	missense	possibly damaging	0.78
R7979:Tns3	UTSW	11	8,442,701 (GRCm39)	missense	probably benign	0.01
R8055:Tns3	UTSW	11	8,495,343 (GRCm39)	missense	probably damaging	1.00
R8057:Tns3	UTSW	11	8,442,773 (GRCm39)	missense	probably benign
R8077:Tns3	UTSW	11	8,395,667 (GRCm39)	missense	probably damaging	1.00
R8523:Tns3	UTSW	11	8,398,779 (GRCm39)	missense	probably damaging	1.00
R8790:Tns3	UTSW	11	8,468,273 (GRCm39)	missense	probably damaging	0.99
R9228:Tns3	UTSW	11	8,400,094 (GRCm39)	missense	probably damaging	1.00
R9374:Tns3	UTSW	11	8,442,606 (GRCm39)	missense	probably damaging	1.00
R9476:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9510:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9594:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9595:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9596:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9624:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9629:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
T0975:Tns3	UTSW	11	8,401,146 (GRCm39)	missense	probably benign	0.00
T0975:Tns3	UTSW	11	8,499,100 (GRCm39)	start gained	probably benign
T0975:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,401,224 (GRCm39)	missense	probably benign	0.00
Z1177:Tns3	UTSW	11	8,401,014 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCATCAGACAGGAGAGAGTTCTG -3'
(R):5'- GCGACTCAGGTCATTCTACTG -3'

Sequencing Primer
(F):5'- CTGGCTGCTCTTGAGACAG -3'
(R):5'- CTCCGCCAGGACTGATAAG -3'

Posted On

2020-10-20