Incidental Mutation 'R0280:Odad4'
ID 37473
Institutional Source Beutler Lab
Gene Symbol Odad4
Ensembl Gene ENSMUSG00000006784
Gene Name outer dynein arm complex subunit 4
Synonyms 4933404O19Rik, Ttc25
MMRRC Submission 038502-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.089) question?
Stock # R0280 (G1)
Quality Score 196
Status Validated
Chromosome 11
Chromosomal Location 100436434-100463392 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 100441091 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Lysine to Asparagine at position 107 (K107N)
Ref Sequence ENSEMBL: ENSMUSP00000090355 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000006976] [ENSMUST00000092684]
AlphaFold Q9D4B2
Predicted Effect probably damaging
Transcript: ENSMUST00000006976
AA Change: K107N

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000006976
Gene: ENSMUSG00000006784
AA Change: K107N

DomainStartEndE-ValueType
TPR 11 44 3.69e1 SMART
TPR 45 78 3.07e1 SMART
TPR 79 112 4.96e0 SMART
low complexity region 147 158 N/A INTRINSIC
low complexity region 191 208 N/A INTRINSIC
TPR 318 351 4.96e0 SMART
TPR 358 391 1.11e1 SMART
Blast:TPR 395 428 7e-14 BLAST
TPR 435 468 2.99e1 SMART
low complexity region 493 505 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000092684
AA Change: K107N

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000090355
Gene: ENSMUSG00000006784
AA Change: K107N

DomainStartEndE-ValueType
TPR 11 44 3.69e1 SMART
TPR 45 78 3.07e1 SMART
TPR 79 112 4.96e0 SMART
low complexity region 147 158 N/A INTRINSIC
low complexity region 191 208 N/A INTRINSIC
TPR 318 351 4.96e0 SMART
TPR 358 391 1.11e1 SMART
Blast:TPR 395 428 5e-14 BLAST
TPR 435 468 2.99e1 SMART
coiled coil region 528 548 N/A INTRINSIC
low complexity region 556 567 N/A INTRINSIC
coiled coil region 599 624 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000127389
Predicted Effect noncoding transcript
Transcript: ENSMUST00000132143
Predicted Effect noncoding transcript
Transcript: ENSMUST00000150331
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 96.9%
  • 20x: 94.6%
Validation Efficiency 96% (55/57)
MGI Phenotype PHENOTYPE: Mice homozygous for a null allele display partial preweaning lethality, impaired ciliary motility, and a variety of left-right body symmetry defects. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 52 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acsbg3 T A 17: 57,192,169 (GRCm39) Y577* probably null Het
Adam18 C T 8: 25,164,070 (GRCm39) G38R probably benign Het
Ankrd16 T G 2: 11,786,312 (GRCm39) V187G probably damaging Het
Ccdc110 A T 8: 46,396,487 (GRCm39) N793Y probably benign Het
Ccdc170 T C 10: 4,508,663 (GRCm39) I629T possibly damaging Het
Clcn6 A G 4: 148,093,172 (GRCm39) L836P probably damaging Het
Colgalt2 G T 1: 152,384,312 (GRCm39) A551S possibly damaging Het
Crocc T C 4: 140,755,737 (GRCm39) E1097G probably damaging Het
Csmd1 A T 8: 16,321,616 (GRCm39) V494E probably damaging Het
Drg1 A T 11: 3,206,537 (GRCm39) probably null Het
Dscam T C 16: 96,840,206 (GRCm39) K134E possibly damaging Het
Dyrk1b T C 7: 27,883,737 (GRCm39) Y198H probably damaging Het
Esr1 A G 10: 4,806,951 (GRCm39) D289G probably benign Het
Esr1 G T 10: 4,889,289 (GRCm39) V396F probably damaging Het
Evi5l T C 8: 4,243,133 (GRCm39) V339A probably damaging Het
Fat4 A T 3: 38,944,965 (GRCm39) Q1286L probably benign Het
Fcsk A T 8: 111,621,380 (GRCm39) V188D probably damaging Het
Frem1 T C 4: 82,887,681 (GRCm39) H1118R probably damaging Het
Fut9 C T 4: 25,619,852 (GRCm39) D321N probably benign Het
Gaa T G 11: 119,175,373 (GRCm39) V917G probably damaging Het
Gm973 GCC GC 1: 59,583,839 (GRCm39) probably null Het
Kidins220 A G 12: 25,060,140 (GRCm39) T767A probably damaging Het
Kif7 A G 7: 79,348,571 (GRCm39) S1257P probably benign Het
Ltn1 A G 16: 87,194,726 (GRCm39) L1391P probably damaging Het
Mast3 A G 8: 71,240,564 (GRCm39) V291A possibly damaging Het
Mast3 A G 8: 71,236,439 (GRCm39) Y681H probably damaging Het
Metrn C T 17: 26,014,109 (GRCm39) R239H probably benign Het
Mphosph10 C A 7: 64,026,451 (GRCm39) K666N possibly damaging Het
Mtbp C T 15: 55,449,857 (GRCm39) T433I probably benign Het
Mtmr2 A G 9: 13,710,545 (GRCm39) K365E probably damaging Het
Nanog A G 6: 122,690,357 (GRCm39) D229G probably damaging Het
Nkapd1 T C 9: 50,520,679 (GRCm39) T123A probably damaging Het
Npepps T C 11: 97,131,840 (GRCm39) N338S possibly damaging Het
Nphp4 T A 4: 152,636,393 (GRCm39) probably benign Het
Or4g16 T C 2: 111,137,417 (GRCm39) F289S possibly damaging Het
Plcl2 A G 17: 50,914,062 (GRCm39) E357G probably damaging Het
Polb A T 8: 23,130,408 (GRCm39) Y173N probably damaging Het
R3hdm1 T A 1: 128,090,512 (GRCm39) S74T probably benign Het
Raet1d T A 10: 22,246,782 (GRCm39) C37S probably damaging Het
Reln G A 5: 22,432,511 (GRCm39) probably benign Het
Rps6kc1 T C 1: 190,541,197 (GRCm39) S369G probably damaging Het
Sgf29 A G 7: 126,270,743 (GRCm39) E108G probably benign Het
Sh3tc1 A G 5: 35,863,361 (GRCm39) L942P probably damaging Het
Slc22a27 A T 19: 7,874,187 (GRCm39) L188* probably null Het
Slc9a3 T A 13: 74,307,543 (GRCm39) I445N probably damaging Het
Sufu A T 19: 46,439,112 (GRCm39) probably benign Het
Tomm40 G A 7: 19,447,676 (GRCm39) T118I probably damaging Het
Ttn C T 2: 76,570,823 (GRCm39) R26690H probably damaging Het
Vmn2r16 A G 5: 109,488,005 (GRCm39) I293V possibly damaging Het
Vmn2r68 T A 7: 84,882,457 (GRCm39) probably benign Het
Vmn2r68 C G 7: 84,882,466 (GRCm39) probably null Het
Vsig8 A G 1: 172,389,105 (GRCm39) D119G probably benign Het
Other mutations in Odad4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02027:Odad4 APN 11 100,460,728 (GRCm39) missense probably damaging 1.00
IGL02379:Odad4 APN 11 100,457,809 (GRCm39) missense possibly damaging 0.87
IGL03091:Odad4 APN 11 100,441,076 (GRCm39) nonsense probably null
IGL02991:Odad4 UTSW 11 100,444,698 (GRCm39) missense probably benign 0.01
R0044:Odad4 UTSW 11 100,457,827 (GRCm39) missense probably damaging 1.00
R0137:Odad4 UTSW 11 100,454,394 (GRCm39) missense probably damaging 1.00
R1373:Odad4 UTSW 11 100,436,658 (GRCm39) missense probably damaging 1.00
R1793:Odad4 UTSW 11 100,460,679 (GRCm39) splice site probably null
R2097:Odad4 UTSW 11 100,454,408 (GRCm39) missense possibly damaging 0.89
R2121:Odad4 UTSW 11 100,457,837 (GRCm39) critical splice donor site probably null
R2509:Odad4 UTSW 11 100,444,361 (GRCm39) missense probably damaging 0.99
R2655:Odad4 UTSW 11 100,444,405 (GRCm39) missense probably damaging 1.00
R4635:Odad4 UTSW 11 100,442,333 (GRCm39) nonsense probably null
R4773:Odad4 UTSW 11 100,440,742 (GRCm39) missense probably benign 0.05
R4858:Odad4 UTSW 11 100,441,147 (GRCm39) missense probably damaging 0.96
R5164:Odad4 UTSW 11 100,462,346 (GRCm39) nonsense probably null
R5181:Odad4 UTSW 11 100,440,719 (GRCm39) missense probably damaging 1.00
R5707:Odad4 UTSW 11 100,444,887 (GRCm39) missense probably damaging 0.99
R5742:Odad4 UTSW 11 100,436,699 (GRCm39) missense possibly damaging 0.90
R7634:Odad4 UTSW 11 100,452,731 (GRCm39) critical splice donor site probably null
R7851:Odad4 UTSW 11 100,436,655 (GRCm39) missense probably damaging 1.00
R8186:Odad4 UTSW 11 100,454,505 (GRCm39) missense probably benign 0.27
R8194:Odad4 UTSW 11 100,454,502 (GRCm39) missense probably benign 0.06
R8444:Odad4 UTSW 11 100,452,731 (GRCm39) critical splice donor site probably null
R8879:Odad4 UTSW 11 100,457,752 (GRCm39) nonsense probably null
X0018:Odad4 UTSW 11 100,444,424 (GRCm39) missense probably damaging 0.99
X0028:Odad4 UTSW 11 100,436,724 (GRCm39) missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- CGCCATTTCCATTGCAGCAGAC -3'
(R):5'- TGTGACCTCAGCAACAGCCAAG -3'

Sequencing Primer
(F):5'- ATTGCAGCAGACTCTGGTTAC -3'
(R):5'- CCAAGTCTAAGTGTGAGAGTCCC -3'
Posted On 2013-05-23