Mutagenetix > Incidental Mutation

Incidental Mutation 'R4893:Adgrf3'

377392

Institutional Source

Beutler Lab

Gene Symbol

Adgrf3

Ensembl Gene

ENSMUSG00000067642

Gene Name

adhesion G protein-coupled receptor F3

Synonyms

Gpr113, LOC381628, PGR23

MMRRC Submission

042498-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4893 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

30193431-30205722 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 30200478 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Alanine at position 286 (D286A)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088117] [ENSMUST00000125367]

AlphaFold

Q58Y75

Predicted Effect

probably benign
Transcript: ENSMUST00000088117
AA Change: D286A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000085440
Gene: ENSMUSG00000067642
AA Change: D286A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	85	97	N/A	INTRINSIC
Blast:IG	163	252	2e-20	BLAST
Blast:CCP	341	399	1e-6	BLAST
low complexity region	403	415	N/A	INTRINSIC
low complexity region	471	483	N/A	INTRINSIC
GPS	632	684	2.68e-17	SMART
Pfam:7tm_2	687	935	1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000114774
AA Change: D286A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000110422
Gene: ENSMUSG00000067642
AA Change: D286A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	85	97	N/A	INTRINSIC
Blast:IG	163	252	2e-20	BLAST
Blast:CCP	341	399	1e-6	BLAST
Pfam:DUF3497	417	609	1.1e-12	PFAM
GPS	631	683	2.68e-17	SMART
Pfam:7tm_2	686	934	5.3e-31	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000125367

SMART Domains

Protein: ENSMUSP00000120958
Gene: ENSMUSG00000067642

Domain	Start	End	E-Value	Type
transmembrane domain	21	43	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135322

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 93.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700030J22Rik	A	G	8: 116,971,165	I401T	probably damaging	Het
2700049A03Rik	A	T	12: 71,164,547	E685V	possibly damaging	Het
2700049A03Rik	G	T	12: 71,164,546	E685*	probably null	Het
Acss1	C	A	2: 150,629,866	V323F	probably damaging	Het
Akap5	T	C	12: 76,329,969	V718A	probably damaging	Het
Ankk1	A	G	9: 49,415,683	V732A	probably benign	Het
Antxr2	T	A	5: 98,004,072	D180V	probably damaging	Het
Arfgef2	T	G	2: 166,866,956	F1063V	probably benign	Het
Ascl5	A	T	1: 136,051,179	I126F	probably damaging	Het
Aspm	T	A	1: 139,489,839		probably null	Het
Atf6b	T	C	17: 34,648,612	S100P	probably damaging	Het
Baz2a	C	A	10: 128,123,415	H1266Q	possibly damaging	Het
Cdh4	A	T	2: 179,847,419		probably benign	Het
Celsr3	C	G	9: 108,849,421	S3283C	probably damaging	Het
Clca4b	C	T	3: 144,925,173	V309M	possibly damaging	Het
Cnbd2	T	C	2: 156,365,184	Y436H	probably damaging	Het
Csnk1a1	T	C	18: 61,585,301		probably benign	Het
Cstf1	C	T	2: 172,380,524	R401C	probably damaging	Het
Cul3	A	G	1: 80,288,850	I117T	probably damaging	Het
Dlgap3	A	G	4: 127,194,983	D124G	probably damaging	Het
Dnah12	A	G	14: 26,710,170	D381G	possibly damaging	Het
Dtna	T	C	18: 23,569,667	L85P	probably damaging	Het
Ephb2	A	G	4: 136,659,753	I722T	probably damaging	Het
Epn3	A	T	11: 94,491,996	F421I	probably damaging	Het
Fam160b1	T	A	19: 57,381,756	H477Q	probably benign	Het
Fam83f	T	A	15: 80,691,955	L269H	probably damaging	Het
Gata4	G	A	14: 63,201,596	A139V	probably benign	Het
Glce	T	C	9: 62,068,495	D241G	probably benign	Het
Gm17728	A	T	17: 9,422,231	I58F	probably benign	Het
Inhba	A	T	13: 16,026,549	D232V	possibly damaging	Het
Itgb2l	C	A	16: 96,427,821	R394L	probably benign	Het
Klhl14	T	C	18: 21,557,935	Y486C	probably damaging	Het
Krt1	A	G	15: 101,850,120	I203T	probably damaging	Het
Lims2	T	C	18: 31,941,811		probably null	Het
Lrrc31	T	G	3: 30,679,297	I423L	probably benign	Het
Map7d1	C	T	4: 126,233,222	D732N	unknown	Het
Mau2	A	T	8: 70,030,640		probably null	Het
Mbtps1	G	A	8: 119,518,193	R840W	probably damaging	Het
Morn3	T	C	5: 123,037,682	I214M	probably damaging	Het
Mrpl44	A	G	1: 79,777,865	K63E	probably damaging	Het
Muc20	T	C	16: 32,794,672	T112A	possibly damaging	Het
Myo6	T	A	9: 80,228,877	L94Q	probably damaging	Het
Nos1	C	A	5: 117,952,877	T1423K	possibly damaging	Het
Olfr166	A	G	16: 19,486,903	T22A	probably benign	Het
Olfr31	T	C	14: 14,328,852	V247A	probably damaging	Het
Pdzd2	T	C	15: 12,385,343	T1114A	probably benign	Het
Pgm1	T	C	5: 64,105,940	V310A	probably benign	Het
Pi4ka	T	C	16: 17,377,036	E166G	probably benign	Het
Pign	A	T	1: 105,646,711	D303E	probably damaging	Het
Pik3c3	G	T	18: 30,282,000	V149L	probably benign	Het
Pkd1l3	A	T	8: 109,638,394	Y1126F	probably benign	Het
Pkd2l1	T	A	19: 44,153,771	Q516L	probably benign	Het
Pnliprp2	C	A	19: 58,771,421	Q355K	probably benign	Het
Pnpla7	T	A	2: 25,053,676	Y1314*	probably null	Het
Ppp1r1b	A	G	11: 98,355,344	T51A	possibly damaging	Het
Prkcd	A	T	14: 30,599,425	S544T	probably damaging	Het
Pusl1	G	A	4: 155,889,541	T252I	probably benign	Het
Rnf44	A	G	13: 54,681,932		probably null	Het
Slc24a2	A	T	4: 87,226,908	V303E	probably damaging	Het
Slc28a2	T	A	2: 122,455,216		probably null	Het
Sox8	T	C	17: 25,568,989	D162G	probably damaging	Het
Spag1	T	C	15: 36,197,846		probably null	Het
Taar6	T	C	10: 23,985,400	I83V	probably benign	Het
Tes	T	A	6: 17,104,596	C359S	probably damaging	Het
Vmn2r4	A	T	3: 64,406,255	L435H	probably damaging	Het
Zfhx3	G	T	8: 108,957,007	D3693Y	unknown	Het
Zfp931	G	A	2: 178,068,203	P130L	probably damaging	Het
Zfr	T	G	15: 12,136,542	V95G	unknown	Het
Zw10	A	G	9: 49,074,025	E587G	possibly damaging	Het

Other mutations in Adgrf3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03080:Adgrf3	APN	5	30,196,829 (GRCm38)	missense	probably benign	0.02
IGL03171:Adgrf3	APN	5	30,196,294 (GRCm38)	missense	probably damaging	1.00
R0010:Adgrf3	UTSW	5	30,205,609 (GRCm38)	splice site	probably benign
R0042:Adgrf3	UTSW	5	30,197,428 (GRCm38)	missense	probably damaging	1.00
R0140:Adgrf3	UTSW	5	30,196,381 (GRCm38)	missense	probably benign	0.19
R0617:Adgrf3	UTSW	5	30,195,080 (GRCm38)	missense	probably benign	0.25
R0748:Adgrf3	UTSW	5	30,196,876 (GRCm38)	missense	probably damaging	1.00
R1291:Adgrf3	UTSW	5	30,199,534 (GRCm38)	missense	probably damaging	0.99
R1330:Adgrf3	UTSW	5	30,195,095 (GRCm38)	missense	probably benign	0.24
R1468:Adgrf3	UTSW	5	30,202,229 (GRCm38)	splice site	probably benign
R1695:Adgrf3	UTSW	5	30,203,555 (GRCm38)	missense	probably benign	0.05
R1716:Adgrf3	UTSW	5	30,197,551 (GRCm38)	missense	probably benign	0.03
R1844:Adgrf3	UTSW	5	30,199,213 (GRCm38)	missense	probably damaging	0.96
R1935:Adgrf3	UTSW	5	30,202,306 (GRCm38)	missense	probably benign	0.00
R1936:Adgrf3	UTSW	5	30,202,306 (GRCm38)	missense	probably benign	0.00
R2059:Adgrf3	UTSW	5	30,199,491 (GRCm38)	missense	possibly damaging	0.91
R2656:Adgrf3	UTSW	5	30,196,438 (GRCm38)	missense	possibly damaging	0.96
R2913:Adgrf3	UTSW	5	30,196,994 (GRCm38)	missense	probably damaging	1.00
R2914:Adgrf3	UTSW	5	30,196,994 (GRCm38)	missense	probably damaging	1.00
R2987:Adgrf3	UTSW	5	30,197,360 (GRCm38)	missense	probably damaging	1.00
R3797:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3798:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3799:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3934:Adgrf3	UTSW	5	30,200,434 (GRCm38)	unclassified	probably benign
R4043:Adgrf3	UTSW	5	30,204,362 (GRCm38)	missense	probably benign	0.00
R4080:Adgrf3	UTSW	5	30,197,369 (GRCm38)	nonsense	probably null
R4575:Adgrf3	UTSW	5	30,202,257 (GRCm38)	missense	probably benign	0.00
R4754:Adgrf3	UTSW	5	30,197,617 (GRCm38)	critical splice acceptor site	probably null
R4819:Adgrf3	UTSW	5	30,198,444 (GRCm38)	missense	possibly damaging	0.66
R4991:Adgrf3	UTSW	5	30,199,148 (GRCm38)	missense	probably benign	0.26
R5686:Adgrf3	UTSW	5	30,197,306 (GRCm38)	missense	probably damaging	1.00
R5965:Adgrf3	UTSW	5	30,205,639 (GRCm38)	missense	probably benign	0.00
R5997:Adgrf3	UTSW	5	30,198,362 (GRCm38)	critical splice donor site	probably null
R6103:Adgrf3	UTSW	5	30,196,267 (GRCm38)	missense	probably damaging	1.00
R6244:Adgrf3	UTSW	5	30,197,533 (GRCm38)	missense	probably benign	0.17
R6409:Adgrf3	UTSW	5	30,197,314 (GRCm38)	missense	probably damaging	0.96
R6575:Adgrf3	UTSW	5	30,196,524 (GRCm38)	missense	possibly damaging	0.72
R6745:Adgrf3	UTSW	5	30,203,603 (GRCm38)	missense	probably benign	0.31
R6790:Adgrf3	UTSW	5	30,196,387 (GRCm38)	missense	probably benign	0.00
R6813:Adgrf3	UTSW	5	30,197,521 (GRCm38)	missense	probably damaging	0.96
R7202:Adgrf3	UTSW	5	30,204,380 (GRCm38)	nonsense	probably null
R7250:Adgrf3	UTSW	5	30,195,682 (GRCm38)	missense	probably damaging	1.00
R7353:Adgrf3	UTSW	5	30,198,497 (GRCm38)	missense	probably damaging	0.98
R7634:Adgrf3	UTSW	5	30,202,247 (GRCm38)	missense	probably benign	0.01
R7658:Adgrf3	UTSW	5	30,197,206 (GRCm38)	missense	probably benign	0.41
R8037:Adgrf3	UTSW	5	30,199,512 (GRCm38)	missense	probably damaging	1.00
R8281:Adgrf3	UTSW	5	30,197,303 (GRCm38)	missense	possibly damaging	0.46
R8717:Adgrf3	UTSW	5	30,198,581 (GRCm38)	unclassified	probably benign
R8857:Adgrf3	UTSW	5	30,197,067 (GRCm38)	nonsense	probably null
R8926:Adgrf3	UTSW	5	30,200,448 (GRCm38)	missense	possibly damaging	0.46
R9391:Adgrf3	UTSW	5	30,195,073 (GRCm38)	missense	possibly damaging	0.94
R9446:Adgrf3	UTSW	5	30,196,959 (GRCm38)	missense	probably benign	0.01
R9522:Adgrf3	UTSW	5	30,199,484 (GRCm38)	missense	possibly damaging	0.90
Z1088:Adgrf3	UTSW	5	30,199,120 (GRCm38)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- TACTGCAGGAGTCAAGTGGG -3'
(R):5'- CTGGGCCGAGACTTAGAAAG -3'

Sequencing Primer
(F):5'- GAGTGTATGATGGGAAGGTTCTATC -3'
(R):5'- CACATCTCTGCAGGTGAGTAC -3'

Posted On

2016-03-17