Mutagenetix > Incidental Mutation

Incidental Mutation 'R5495:Taar4'

432298

Institutional Source

Beutler Lab

Gene Symbol

Taar4

Ensembl Gene

ENSMUSG00000069707

Gene Name

trace amine-associated receptor 4

Synonyms

MMRRC Submission

043056-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R5495 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

23836392-23837435 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 23837181 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 264 (I264F)

Ref Sequence

ENSEMBL: ENSMUSP00000090330 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092660]

AlphaFold

Q5QD15

Predicted Effect

possibly damaging
Transcript: ENSMUST00000092660
AA Change: I264F

PolyPhen 2 Score 0.951 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000090330
Gene: ENSMUSG00000069707
AA Change: I264F

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	44	328	9.2e-11	PFAM
Pfam:7tm_1	50	313	4.6e-63	PFAM

Coding Region Coverage

1x: 98.4%
3x: 97.3%
10x: 95.3%
20x: 91.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired olfactory response and aversion to PEA and puma urine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akt2	T	C	7: 27,335,594 (GRCm39)		probably null	Het
Arhgap29	T	G	3: 121,808,578 (GRCm39)	M844R	probably damaging	Het
Atp1a1	T	G	3: 101,498,741 (GRCm39)	D184A	probably benign	Het
Bcl11a	T	A	11: 24,115,042 (GRCm39)	V795E	possibly damaging	Het
Casp12	T	A	9: 5,353,797 (GRCm39)	I277N	possibly damaging	Het
Ccdc59	A	G	10: 105,681,239 (GRCm39)	K164E	probably damaging	Het
D630003M21Rik	C	T	2: 158,062,431 (GRCm39)	G30S	possibly damaging	Het
Dgkd	A	G	1: 87,854,594 (GRCm39)	D632G	probably damaging	Het
Efr3a	A	G	15: 65,687,258 (GRCm39)	K56E	possibly damaging	Het
Egflam	T	A	15: 7,280,722 (GRCm39)	R434S	probably damaging	Het
Fancl	C	G	11: 26,347,801 (GRCm39)	A51G	probably damaging	Het
Fkbp7	T	C	2: 76,493,638 (GRCm39)	Y185C	probably damaging	Het
Galc	A	G	12: 98,197,673 (GRCm39)		probably null	Het
Galnt15	A	G	14: 31,751,774 (GRCm39)	S109G	probably damaging	Het
Gramd2b	T	C	18: 56,615,694 (GRCm39)	I163T	probably damaging	Het
Impa1	A	G	3: 10,391,230 (GRCm39)	V80A	probably benign	Het
Itga10	T	C	3: 96,554,687 (GRCm39)	M56T	possibly damaging	Het
Larp1b	G	T	3: 40,990,257 (GRCm39)	R135I	probably damaging	Het
Lgals12	C	T	19: 7,581,495 (GRCm39)	A71T	probably damaging	Het
Lmbr1	A	T	5: 29,551,851 (GRCm39)	L78*	probably null	Het
Lrat	C	A	3: 82,804,289 (GRCm39)	M229I	probably benign	Het
Mug2	A	T	6: 122,056,609 (GRCm39)	M1185L	probably damaging	Het
Naprt	T	C	15: 75,765,696 (GRCm39)		probably null	Het
Nfat5	A	G	8: 108,095,079 (GRCm39)	I1107V	probably benign	Het
Nr4a2	T	C	2: 57,002,387 (GRCm39)	Y22C	probably damaging	Het
Ogfod1	C	A	8: 94,790,906 (GRCm39)	Q526K	probably benign	Het
Or2d4	C	A	7: 106,543,699 (GRCm39)	G170*	probably null	Het
Or3a10	G	A	11: 73,935,611 (GRCm39)	T163I	probably damaging	Het
Or4p21	T	C	2: 88,276,401 (GRCm39)	T294A	probably benign	Het
Or8g36	T	C	9: 39,422,441 (GRCm39)	T192A	probably benign	Het
Parp10	T	G	15: 76,127,366 (GRCm39)	I24L	probably benign	Het
Pcdha11	A	G	18: 37,144,079 (GRCm39)	T57A	probably benign	Het
Prdm8	A	G	5: 98,333,165 (GRCm39)	E244G	possibly damaging	Het
Prl6a1	T	C	13: 27,496,654 (GRCm39)	S3P	possibly damaging	Het
Rab11fip3	T	A	17: 26,235,117 (GRCm39)	T18S	probably damaging	Het
Rfc4	T	C	16: 22,941,004 (GRCm39)		probably benign	Het
Rubcnl	G	T	14: 75,279,777 (GRCm39)	V387F	possibly damaging	Het
S100a7l2	A	T	3: 90,997,602 (GRCm39)	L38M	possibly damaging	Het
Serpinb12	T	C	1: 106,884,151 (GRCm39)	L299P	probably damaging	Het
Sptbn5	G	A	2: 119,876,965 (GRCm39)		probably benign	Het
Tdpoz4	A	T	3: 93,704,806 (GRCm39)	T368S	probably benign	Het
Thsd7b	T	A	1: 129,523,570 (GRCm39)	H124Q	probably damaging	Het
Ugt1a6a	A	T	1: 88,066,746 (GRCm39)	Q184L	probably benign	Het
Vnn1	T	A	10: 23,774,462 (GRCm39)	F168L	probably damaging	Het
Zan	A	G	5: 137,468,670 (GRCm39)	L267P	probably damaging	Het
Zswim8	A	G	14: 20,772,354 (GRCm39)	S1621G	probably damaging	Het

Other mutations in Taar4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02576:Taar4	APN	10	23,836,909 (GRCm39)	missense	probably damaging	1.00
IGL03202:Taar4	APN	10	23,836,692 (GRCm39)	missense	probably damaging	1.00
I2288:Taar4	UTSW	10	23,836,818 (GRCm39)	missense	probably benign	0.03
R0103:Taar4	UTSW	10	23,837,304 (GRCm39)	missense	probably damaging	1.00
R0103:Taar4	UTSW	10	23,837,304 (GRCm39)	missense	probably damaging	1.00
R0514:Taar4	UTSW	10	23,836,780 (GRCm39)	missense	probably damaging	1.00
R1222:Taar4	UTSW	10	23,837,230 (GRCm39)	missense	probably benign	0.05
R1248:Taar4	UTSW	10	23,836,936 (GRCm39)	missense	possibly damaging	0.95
R1514:Taar4	UTSW	10	23,836,510 (GRCm39)	missense	possibly damaging	0.71
R1921:Taar4	UTSW	10	23,837,239 (GRCm39)	missense	probably damaging	1.00
R2074:Taar4	UTSW	10	23,837,071 (GRCm39)	missense	probably benign	0.18
R2354:Taar4	UTSW	10	23,836,912 (GRCm39)	missense	probably damaging	1.00
R2392:Taar4	UTSW	10	23,837,172 (GRCm39)	missense	possibly damaging	0.94
R2698:Taar4	UTSW	10	23,837,328 (GRCm39)	missense	probably damaging	1.00
R3902:Taar4	UTSW	10	23,836,913 (GRCm39)	missense	probably damaging	1.00
R4688:Taar4	UTSW	10	23,836,731 (GRCm39)	missense	probably damaging	1.00
R5595:Taar4	UTSW	10	23,836,639 (GRCm39)	missense	probably damaging	1.00
R5773:Taar4	UTSW	10	23,837,056 (GRCm39)	missense	probably damaging	1.00
R7403:Taar4	UTSW	10	23,836,957 (GRCm39)	missense	probably damaging	1.00
R7581:Taar4	UTSW	10	23,837,052 (GRCm39)	missense	probably damaging	0.97
R7736:Taar4	UTSW	10	23,836,897 (GRCm39)	missense	probably damaging	1.00
R7859:Taar4	UTSW	10	23,837,032 (GRCm39)	missense	probably benign	0.35
R8676:Taar4	UTSW	10	23,836,801 (GRCm39)	missense	possibly damaging	0.56

Predicted Primers

PCR Primer
(F):5'- ACAAGCTCTGGGGAGTTCTG -3'
(R):5'- TCCTGTGACTATCATCCTCAGGG -3'

Sequencing Primer
(F):5'- GGAGTTCTGGCTTCCTTCATAGC -3'
(R):5'- TCAGGGCCTTTCGAAACCAG -3'

Posted On

2016-10-05