Mutagenetix > Incidental Mutation

Incidental Mutation 'R7810:Wdfy3'

601076

Institutional Source

Beutler Lab

Gene Symbol

Wdfy3

Ensembl Gene

ENSMUSG00000043940

Gene Name

WD repeat and FYVE domain containing 3

Synonyms

2610509D04Rik, Ggtb3, Bchs, D5Ertd66e, Bwf1, Alfy

MMRRC Submission

045865-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.942) question?

Stock #

R7810 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

101980822-102217787 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 102099265 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 261 (L261*)

Ref Sequence

ENSEMBL: ENSMUSP00000052607 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053177] [ENSMUST00000174598] [ENSMUST00000174698] [ENSMUST00000212024]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000053177
AA Change: L261*

SMART Domains

Protein: ENSMUSP00000052607
Gene: ENSMUSG00000043940
AA Change: L261*

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
low complexity region	1408	1417	N/A	INTRINSIC
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2517	2638	3.1e-17	PFAM
Beach	2677	2958	2.54e-217	SMART
WD40	3054	3088	1.28e1	SMART
WD40	3098	3137	7.73e-6	SMART
WD40	3140	3178	8.29e-1	SMART
WD40	3183	3227	3.09e-1	SMART
low complexity region	3253	3274	N/A	INTRINSIC
low complexity region	3307	3318	N/A	INTRINSIC
WD40	3381	3420	1.33e1	SMART
FYVE	3428	3497	3.18e-27	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000174598
AA Change: L261*

SMART Domains

Protein: ENSMUSP00000134244
Gene: ENSMUSG00000043940
AA Change: L261*

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
Pfam:DUF4704	1392	1597	6.6e-11	PFAM
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2588	2656	1.8e-14	PFAM
Beach	2695	2976	2.54e-217	SMART
WD40	3072	3106	1.28e1	SMART
WD40	3116	3155	7.73e-6	SMART
WD40	3158	3196	8.29e-1	SMART
WD40	3201	3245	3.09e-1	SMART
low complexity region	3271	3292	N/A	INTRINSIC
low complexity region	3325	3336	N/A	INTRINSIC
WD40	3399	3438	1.33e1	SMART
FYVE	3446	3515	3.18e-27	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000174698
AA Change: L261*

SMART Domains

Protein: ENSMUSP00000134541
Gene: ENSMUSG00000043940
AA Change: L261*

Domain	Start	End	E-Value	Type
Blast:WD40	235	281	2e-21	BLAST
low complexity region	463	481	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000212024
AA Change: L261*

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (91/92)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]
PHENOTYPE: Mice homozygous for hypomorphic mutations of this gene exhibit perinatal lethality, altered neural progenitor divisions and neuronal migration, a regionally enlarged cerebral cortex, and focal cortical dysplasias. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam34	T	C	8: 44,105,045 (GRCm39)	E200G	probably benign	Het
Ankfy1	C	T	11: 72,645,281 (GRCm39)	Q787*	probably null	Het
Birc6	T	A	17: 74,855,815 (GRCm39)	H208Q	probably damaging	Het
Brsk2	T	A	7: 141,539,157 (GRCm39)		probably null	Het
Cabp5	T	A	7: 13,132,263 (GRCm39)	F11I	possibly damaging	Het
Ccdc88a	T	C	11: 29,435,964 (GRCm39)	Y1296H	probably damaging	Het
Ccdc88b	A	G	19: 6,826,454 (GRCm39)	V1087A	probably benign	Het
Cct3	C	T	3: 88,228,442 (GRCm39)	T508I	probably damaging	Het
Ceacam9	T	A	7: 16,457,658 (GRCm39)	M57K	possibly damaging	Het
Ces1f	T	C	8: 93,983,546 (GRCm39)	E487G	probably damaging	Het
Chd5	A	G	4: 152,443,032 (GRCm39)	K278E	probably damaging	Het
Cntnap3	T	A	13: 64,941,122 (GRCm39)	H286L	possibly damaging	Het
Dnai7	T	G	6: 145,140,312 (GRCm39)	D163A	probably benign	Het
Efr3a	G	T	15: 65,659,022 (GRCm39)		probably benign	Het
Fastkd2	T	A	1: 63,770,851 (GRCm39)	I69N	possibly damaging	Het
Flg2	T	C	3: 93,107,548 (GRCm39)	I11T	possibly damaging	Het
Folr2	T	C	7: 101,490,102 (GRCm39)	M84V	possibly damaging	Het
Galnt12	T	C	4: 47,113,786 (GRCm39)	F360S	probably damaging	Het
Galr2	T	C	11: 116,173,946 (GRCm39)	V192A	probably benign	Het
Gas6	A	T	8: 13,516,809 (GRCm39)	I563N	probably damaging	Het
Gbe1	T	C	16: 70,324,085 (GRCm39)	F567L	possibly damaging	Het
Gm12695	G	A	4: 96,619,608 (GRCm39)	H423Y	probably damaging	Het
Gm6902	T	C	7: 22,973,243 (GRCm39)	T95A	probably benign	Het
Gpr155	G	T	2: 73,212,296 (GRCm39)	A109D	probably damaging	Het
Hsdl1	T	C	8: 120,294,711 (GRCm39)	D5G	probably damaging	Het
Hyal1	C	T	9: 107,455,628 (GRCm39)	P313S	probably damaging	Het
Igfn1	G	A	1: 135,902,527 (GRCm39)	T390M	probably damaging	Het
Irx1	C	A	13: 72,107,917 (GRCm39)	R255L	probably benign	Het
Itga2	T	A	13: 115,002,715 (GRCm39)	T592S	probably benign	Het
Kit	C	A	5: 75,769,982 (GRCm39)	S131R	probably benign	Het
Map1b	T	C	13: 99,568,390 (GRCm39)	T1444A	unknown	Het
Mapk8ip1	C	T	2: 92,219,496 (GRCm39)	E112K	probably benign	Het
Marveld3	T	C	8: 110,681,266 (GRCm39)	I210V	probably damaging	Het
Masp1	T	A	16: 23,295,068 (GRCm39)	I398L	probably benign	Het
Mmrn1	T	C	6: 60,953,309 (GRCm39)	I530T	probably benign	Het
Mtss2	A	G	8: 111,452,833 (GRCm39)	Y26C	probably damaging	Het
Myo5a	T	A	9: 75,067,747 (GRCm39)	S600R	probably benign	Het
Myo5a	A	G	9: 75,076,292 (GRCm39)	I836V	probably benign	Het
Naaladl1	A	G	19: 6,159,694 (GRCm39)	D375G	probably damaging	Het
Napepld	T	A	5: 21,888,263 (GRCm39)	D62V	possibly damaging	Het
Nedd9	A	C	13: 41,465,483 (GRCm39)	I719S	possibly damaging	Het
Nes	T	A	3: 87,882,923 (GRCm39)	M394K	probably benign	Het
Nlrc5	A	T	8: 95,231,772 (GRCm39)	Y1288F	possibly damaging	Het
Nlrp14	T	A	7: 106,791,782 (GRCm39)	C822*	probably null	Het
Noa1	A	C	5: 77,457,071 (GRCm39)	L278R	probably damaging	Het
Nop16	A	G	13: 54,737,889 (GRCm39)		probably benign	Het
Odr4	T	C	1: 150,268,659 (GRCm39)		probably benign	Het
Or11h7	A	T	14: 50,891,450 (GRCm39)	Y252F	probably benign	Het
Or5b12	A	G	19: 12,897,229 (GRCm39)	V148A	probably benign	Het
Osbpl10	C	T	9: 114,890,962 (GRCm39)	H117Y	probably benign	Het
Oxct1	A	T	15: 4,077,058 (GRCm39)	E130D	probably benign	Het
Padi2	A	G	4: 140,676,575 (GRCm39)	E571G	possibly damaging	Het
Pcdhac2	G	A	18: 37,278,717 (GRCm39)	V566M	probably benign	Het
Pdcd11	G	A	19: 47,086,659 (GRCm39)	E222K	possibly damaging	Het
Pira12	C	A	7: 3,897,204 (GRCm39)	C544F	probably damaging	Het
Plekha2	A	G	8: 25,578,356 (GRCm39)		probably null	Het
Pogz	T	C	3: 94,777,418 (GRCm39)	L366P	probably benign	Het
Ppp4r3b	A	G	11: 29,138,086 (GRCm39)	I145V	probably benign	Het
Prkcd	A	G	14: 30,320,407 (GRCm39)		probably null	Het
Ptprk	T	A	10: 28,468,853 (GRCm39)	H1438Q	probably damaging	Het
Rbm4	A	T	19: 4,842,650 (GRCm39)	V63E	possibly damaging	Het
Sbk2	T	A	7: 4,961,938 (GRCm39)	H116L	probably damaging	Het
Sdk1	A	G	5: 141,923,434 (GRCm39)	T352A	probably benign	Het
Septin9	T	A	11: 117,250,264 (GRCm39)	C529*	probably null	Het
Setx	T	A	2: 29,038,663 (GRCm39)	V1716D	probably benign	Het
Slc19a3	C	T	1: 82,997,162 (GRCm39)	V349I	probably benign	Het
Slc27a4	C	A	2: 29,695,722 (GRCm39)	R86S	probably benign	Het
Slc4a8	A	G	15: 100,696,059 (GRCm39)	H613R	possibly damaging	Het
Smoc2	A	G	17: 14,545,884 (GRCm39)	R58G	probably damaging	Het
Sphkap	T	A	1: 83,254,021 (GRCm39)	N1243Y	probably damaging	Het
Srsf5	T	C	12: 80,996,720 (GRCm39)	S261P	unknown	Het
Tab1	A	T	15: 80,042,999 (GRCm39)	T398S	possibly damaging	Het
Tas2r131	T	A	6: 132,934,705 (GRCm39)	T35S	probably benign	Het
Tasor2	T	C	13: 3,625,714 (GRCm39)	K1412R	possibly damaging	Het
Tigd4	T	A	3: 84,502,310 (GRCm39)	V409E	possibly damaging	Het
Tlr12	T	C	4: 128,510,501 (GRCm39)	Q583R	probably benign	Het
Tmprss9	T	A	10: 80,733,145 (GRCm39)	C894S	unknown	Het
Topaz1	T	A	9: 122,578,250 (GRCm39)	S387T	probably benign	Het
Tsfm	T	C	10: 126,847,558 (GRCm39)	R178G	probably benign	Het
Ttll13	T	C	7: 79,902,875 (GRCm39)	I181T	probably damaging	Het
Ttyh3	T	A	5: 140,610,896 (GRCm39)	K97N		Het
Ubr4	A	G	4: 139,142,394 (GRCm39)	E38G		Het
Ugt2b37	T	C	5: 87,402,118 (GRCm39)	Y171C	probably damaging	Het
Unc5b	C	A	10: 60,601,020 (GRCm39)	M935I	probably benign	Het
Usp34	T	C	11: 23,362,314 (GRCm39)	S1606P		Het
Vmn1r195	T	C	13: 22,463,244 (GRCm39)	L238P	probably damaging	Het
Vmn2r85	T	A	10: 130,261,081 (GRCm39)	M419L	probably benign	Het
Washc2	G	T	6: 116,236,020 (GRCm39)	A1164S	probably benign	Het
Wdr64	A	T	1: 175,559,092 (GRCm39)	Y285F	probably benign	Het
Wtap	A	G	17: 13,199,797 (GRCm39)	L62P	probably damaging	Het
Zfp1007	A	C	5: 109,825,170 (GRCm39)	Y93*	probably null	Het
Zfp94	C	A	7: 24,002,498 (GRCm39)	V315F	probably benign	Het
Zhx1	A	T	15: 57,911,798 (GRCm39)		probably null	Het

Other mutations in Wdfy3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Wdfy3	APN	5	102,063,204 (GRCm39)	critical splice donor site	probably null
IGL00567:Wdfy3	APN	5	102,059,896 (GRCm39)	splice site	probably benign
IGL01288:Wdfy3	APN	5	102,049,857 (GRCm39)	splice site	probably null
IGL01323:Wdfy3	APN	5	102,042,930 (GRCm39)	missense	probably damaging	1.00
IGL01352:Wdfy3	APN	5	102,091,986 (GRCm39)	missense	probably damaging	1.00
IGL01553:Wdfy3	APN	5	102,047,897 (GRCm39)	missense	probably benign
IGL01560:Wdfy3	APN	5	102,105,352 (GRCm39)	nonsense	probably null
IGL01566:Wdfy3	APN	5	102,044,454 (GRCm39)	splice site	probably benign
IGL01616:Wdfy3	APN	5	102,061,126 (GRCm39)	missense	probably damaging	0.97
IGL01630:Wdfy3	APN	5	102,055,354 (GRCm39)	missense	probably benign
IGL01791:Wdfy3	APN	5	102,085,278 (GRCm39)	missense	probably damaging	1.00
IGL01820:Wdfy3	APN	5	102,071,947 (GRCm39)	missense	probably benign	0.11
IGL01953:Wdfy3	APN	5	102,042,894 (GRCm39)	nonsense	probably null
IGL02121:Wdfy3	APN	5	102,046,376 (GRCm39)	missense	possibly damaging	0.85
IGL02167:Wdfy3	APN	5	102,109,023 (GRCm39)	missense	probably damaging	0.98
IGL02321:Wdfy3	APN	5	102,070,475 (GRCm39)	missense	probably damaging	0.99
IGL02327:Wdfy3	APN	5	102,036,058 (GRCm39)	missense	probably damaging	1.00
IGL02651:Wdfy3	APN	5	102,044,341 (GRCm39)	missense	probably benign	0.37
IGL02801:Wdfy3	APN	5	102,055,453 (GRCm39)	missense	probably damaging	1.00
IGL02839:Wdfy3	APN	5	102,116,786 (GRCm39)	missense	probably damaging	1.00
IGL02870:Wdfy3	APN	5	102,003,337 (GRCm39)	missense	probably damaging	1.00
IGL02997:Wdfy3	APN	5	102,042,778 (GRCm39)	missense	probably null	1.00
IGL03064:Wdfy3	APN	5	102,083,863 (GRCm39)	missense	probably damaging	0.99
IGL03090:Wdfy3	APN	5	102,014,142 (GRCm39)	missense	probably damaging	1.00
IGL03211:Wdfy3	APN	5	101,992,778 (GRCm39)	splice site	probably benign
IGL03237:Wdfy3	APN	5	101,992,465 (GRCm39)	missense	probably damaging	1.00
IGL03264:Wdfy3	APN	5	102,048,016 (GRCm39)	missense	probably damaging	1.00
Esurient	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
IGL02988:Wdfy3	UTSW	5	102,077,847 (GRCm39)	missense	probably damaging	0.99
PIT4382001:Wdfy3	UTSW	5	102,030,827 (GRCm39)	frame shift	probably null
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0025:Wdfy3	UTSW	5	101,992,912 (GRCm39)	missense	probably damaging	0.98
R0031:Wdfy3	UTSW	5	102,037,161 (GRCm39)	missense	probably damaging	0.97
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0053:Wdfy3	UTSW	5	101,992,480 (GRCm39)	missense	probably damaging	0.97
R0078:Wdfy3	UTSW	5	102,035,971 (GRCm39)	missense	possibly damaging	0.57
R0147:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0148:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0279:Wdfy3	UTSW	5	102,015,958 (GRCm39)	missense	probably damaging	1.00
R0380:Wdfy3	UTSW	5	102,096,832 (GRCm39)	missense	probably damaging	0.99
R0472:Wdfy3	UTSW	5	102,105,309 (GRCm39)	missense	probably benign	0.13
R0513:Wdfy3	UTSW	5	102,038,655 (GRCm39)	missense	probably damaging	0.96
R0594:Wdfy3	UTSW	5	102,054,051 (GRCm39)	missense	possibly damaging	0.94
R0601:Wdfy3	UTSW	5	101,984,038 (GRCm39)	missense	probably benign
R0787:Wdfy3	UTSW	5	102,105,254 (GRCm39)	missense	probably damaging	1.00
R0825:Wdfy3	UTSW	5	102,017,917 (GRCm39)	missense	probably damaging	1.00
R1122:Wdfy3	UTSW	5	102,030,832 (GRCm39)	missense	possibly damaging	0.94
R1167:Wdfy3	UTSW	5	102,023,797 (GRCm39)	missense	probably benign
R1350:Wdfy3	UTSW	5	102,046,418 (GRCm39)	missense	probably damaging	1.00
R1422:Wdfy3	UTSW	5	102,032,080 (GRCm39)	splice site	probably benign
R1446:Wdfy3	UTSW	5	101,999,176 (GRCm39)	missense	possibly damaging	0.68
R1452:Wdfy3	UTSW	5	102,085,604 (GRCm39)	missense	possibly damaging	0.91
R1457:Wdfy3	UTSW	5	102,065,445 (GRCm39)	missense	possibly damaging	0.57
R1543:Wdfy3	UTSW	5	101,991,947 (GRCm39)	missense	probably benign
R1633:Wdfy3	UTSW	5	102,129,414 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy3	UTSW	5	102,023,781 (GRCm39)	missense	possibly damaging	0.62
R1656:Wdfy3	UTSW	5	102,089,313 (GRCm39)	missense	probably damaging	1.00
R1720:Wdfy3	UTSW	5	102,074,391 (GRCm39)	frame shift	probably null
R1743:Wdfy3	UTSW	5	101,991,931 (GRCm39)	missense	probably benign	0.12
R1745:Wdfy3	UTSW	5	102,096,795 (GRCm39)	missense	probably damaging	0.96
R1850:Wdfy3	UTSW	5	102,042,865 (GRCm39)	missense	probably damaging	1.00
R1852:Wdfy3	UTSW	5	102,063,242 (GRCm39)	missense	probably benign	0.00
R1854:Wdfy3	UTSW	5	102,036,052 (GRCm39)	missense	probably benign	0.05
R1880:Wdfy3	UTSW	5	102,065,301 (GRCm39)	missense	probably benign	0.05
R1930:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1931:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1956:Wdfy3	UTSW	5	102,067,275 (GRCm39)	missense	probably benign	0.30
R1965:Wdfy3	UTSW	5	102,099,178 (GRCm39)	missense	probably damaging	1.00
R1997:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R2015:Wdfy3	UTSW	5	102,008,352 (GRCm39)	missense	probably null	1.00
R2087:Wdfy3	UTSW	5	102,042,926 (GRCm39)	missense	probably damaging	1.00
R2156:Wdfy3	UTSW	5	102,046,291 (GRCm39)	critical splice donor site	probably null
R2192:Wdfy3	UTSW	5	102,055,408 (GRCm39)	missense	possibly damaging	0.55
R2313:Wdfy3	UTSW	5	102,037,150 (GRCm39)	missense	probably damaging	1.00
R2332:Wdfy3	UTSW	5	102,036,189 (GRCm39)	splice site	probably benign
R2406:Wdfy3	UTSW	5	102,036,125 (GRCm39)	missense	probably damaging	1.00
R2679:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R2857:Wdfy3	UTSW	5	102,023,796 (GRCm39)	missense	probably benign	0.04
R2937:Wdfy3	UTSW	5	102,091,988 (GRCm39)	missense	probably benign	0.07
R3765:Wdfy3	UTSW	5	102,009,266 (GRCm39)	missense	probably damaging	1.00
R3795:Wdfy3	UTSW	5	102,085,466 (GRCm39)	missense	probably damaging	1.00
R3937:Wdfy3	UTSW	5	102,092,105 (GRCm39)	nonsense	probably null
R3947:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R4024:Wdfy3	UTSW	5	102,071,961 (GRCm39)	splice site	probably benign
R4065:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4066:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4110:Wdfy3	UTSW	5	102,047,924 (GRCm39)	critical splice donor site	probably null
R4235:Wdfy3	UTSW	5	102,070,500 (GRCm39)	critical splice acceptor site	probably null
R4420:Wdfy3	UTSW	5	102,058,850 (GRCm39)	missense	probably damaging	0.97
R4620:Wdfy3	UTSW	5	102,054,011 (GRCm39)	missense	probably damaging	0.99
R4624:Wdfy3	UTSW	5	102,031,949 (GRCm39)	missense	possibly damaging	0.52
R4626:Wdfy3	UTSW	5	102,091,800 (GRCm39)	missense	probably damaging	1.00
R4727:Wdfy3	UTSW	5	102,077,894 (GRCm39)	missense	probably damaging	0.99
R4794:Wdfy3	UTSW	5	102,091,809 (GRCm39)	missense	probably damaging	1.00
R4869:Wdfy3	UTSW	5	102,042,787 (GRCm39)	missense	probably damaging	0.98
R4971:Wdfy3	UTSW	5	102,096,838 (GRCm39)	nonsense	probably null
R4973:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4976:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4984:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4986:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R5068:Wdfy3	UTSW	5	102,042,803 (GRCm39)	missense	probably benign	0.15
R5105:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R5120:Wdfy3	UTSW	5	102,015,972 (GRCm39)	missense	possibly damaging	0.85
R5134:Wdfy3	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
R5139:Wdfy3	UTSW	5	101,997,133 (GRCm39)	critical splice donor site	probably null
R5235:Wdfy3	UTSW	5	101,994,972 (GRCm39)	missense	probably null	0.03
R5303:Wdfy3	UTSW	5	102,100,849 (GRCm39)	missense	probably damaging	1.00
R5368:Wdfy3	UTSW	5	102,020,724 (GRCm39)	missense	probably damaging	1.00
R5426:Wdfy3	UTSW	5	102,067,312 (GRCm39)	missense	probably damaging	0.97
R5442:Wdfy3	UTSW	5	102,044,425 (GRCm39)	missense	probably benign	0.04
R5487:Wdfy3	UTSW	5	101,984,140 (GRCm39)	missense	probably damaging	1.00
R5509:Wdfy3	UTSW	5	102,009,314 (GRCm39)	missense	possibly damaging	0.69
R5877:Wdfy3	UTSW	5	102,017,855 (GRCm39)	missense	probably damaging	1.00
R5988:Wdfy3	UTSW	5	102,032,004 (GRCm39)	missense	probably benign	0.00
R6017:Wdfy3	UTSW	5	101,999,225 (GRCm39)	missense	probably benign	0.01
R6019:Wdfy3	UTSW	5	101,997,289 (GRCm39)	missense	probably damaging	1.00
R6199:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6228:Wdfy3	UTSW	5	102,046,295 (GRCm39)	missense	possibly damaging	0.67
R6258:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6259:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6298:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R6479:Wdfy3	UTSW	5	102,061,045 (GRCm39)	missense	probably damaging	1.00
R6550:Wdfy3	UTSW	5	102,101,032 (GRCm39)	missense	probably benign	0.19
R6776:Wdfy3	UTSW	5	102,031,911 (GRCm39)	missense	possibly damaging	0.57
R6793:Wdfy3	UTSW	5	102,065,297 (GRCm39)	nonsense	probably null
R6809:Wdfy3	UTSW	5	102,071,813 (GRCm39)	missense	possibly damaging	0.63
R6836:Wdfy3	UTSW	5	102,100,865 (GRCm39)	missense	probably damaging	1.00
R6897:Wdfy3	UTSW	5	101,991,932 (GRCm39)	missense	probably benign	0.10
R7014:Wdfy3	UTSW	5	102,042,775 (GRCm39)	critical splice donor site	probably null
R7034:Wdfy3	UTSW	5	102,055,384 (GRCm39)	missense	probably damaging	1.00
R7035:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R7135:Wdfy3	UTSW	5	102,063,303 (GRCm39)	missense	probably damaging	1.00
R7182:Wdfy3	UTSW	5	102,091,758 (GRCm39)	missense	possibly damaging	0.51
R7217:Wdfy3	UTSW	5	102,049,785 (GRCm39)	missense	probably damaging	1.00
R7236:Wdfy3	UTSW	5	101,984,074 (GRCm39)	missense	probably damaging	0.99
R7264:Wdfy3	UTSW	5	102,003,389 (GRCm39)	missense	probably benign	0.02
R7418:Wdfy3	UTSW	5	102,105,366 (GRCm39)	missense	probably benign	0.08
R7533:Wdfy3	UTSW	5	102,030,354 (GRCm39)	missense	probably benign	0.27
R7543:Wdfy3	UTSW	5	102,083,925 (GRCm39)	missense	probably benign	0.00
R7625:Wdfy3	UTSW	5	102,003,252 (GRCm39)	splice site	probably null
R7788:Wdfy3	UTSW	5	101,996,223 (GRCm39)	missense	probably damaging	0.99
R7810:Wdfy3	UTSW	5	102,042,940 (GRCm39)	missense	probably benign	0.01
R8204:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.00
R8268:Wdfy3	UTSW	5	102,089,476 (GRCm39)	missense	probably damaging	1.00
R8286:Wdfy3	UTSW	5	102,085,287 (GRCm39)	missense	probably benign
R8507:Wdfy3	UTSW	5	102,020,767 (GRCm39)	missense	probably benign	0.05
R8514:Wdfy3	UTSW	5	101,999,219 (GRCm39)	missense	possibly damaging	0.92
R8536:Wdfy3	UTSW	5	102,033,064 (GRCm39)	missense	probably benign
R8710:Wdfy3	UTSW	5	102,030,349 (GRCm39)	missense	probably damaging	1.00
R8735:Wdfy3	UTSW	5	102,077,951 (GRCm39)	missense	probably benign	0.00
R8749:Wdfy3	UTSW	5	102,030,446 (GRCm39)	missense	probably damaging	1.00
R8931:Wdfy3	UTSW	5	102,065,421 (GRCm39)	missense	probably benign	0.11
R8943:Wdfy3	UTSW	5	101,993,231 (GRCm39)	intron	probably benign
R8968:Wdfy3	UTSW	5	102,011,983 (GRCm39)	missense	probably benign	0.05
R8979:Wdfy3	UTSW	5	102,096,764 (GRCm39)	missense	probably damaging	1.00
R8998:Wdfy3	UTSW	5	101,993,058 (GRCm39)	missense	probably benign	0.05
R9045:Wdfy3	UTSW	5	101,995,040 (GRCm39)	missense	probably damaging	1.00
R9068:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.34
R9105:Wdfy3	UTSW	5	102,030,512 (GRCm39)	missense	probably benign	0.05
R9122:Wdfy3	UTSW	5	102,091,831 (GRCm39)	missense	probably damaging	1.00
R9209:Wdfy3	UTSW	5	102,078,830 (GRCm39)	missense	probably benign	0.01
R9249:Wdfy3	UTSW	5	101,996,359 (GRCm39)	missense	possibly damaging	0.82
R9348:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R9481:Wdfy3	UTSW	5	102,000,478 (GRCm39)	missense	probably benign	0.19
R9490:Wdfy3	UTSW	5	102,078,716 (GRCm39)	missense	probably benign	0.29
R9524:Wdfy3	UTSW	5	102,055,333 (GRCm39)	missense	probably benign	0.03
R9545:Wdfy3	UTSW	5	102,100,957 (GRCm39)	missense
R9548:Wdfy3	UTSW	5	102,033,059 (GRCm39)	missense	probably damaging	0.99
R9636:Wdfy3	UTSW	5	102,047,899 (GRCm39)	missense	probably benign
R9750:Wdfy3	UTSW	5	102,077,960 (GRCm39)	missense	probably benign	0.00
R9766:Wdfy3	UTSW	5	102,042,866 (GRCm39)	missense	possibly damaging	0.90
R9771:Wdfy3	UTSW	5	102,000,195 (GRCm39)	missense	probably damaging	1.00
Z1177:Wdfy3	UTSW	5	102,048,107 (GRCm39)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- GGGTTTACCTGAGCAAGAGGTC -3'
(R):5'- AAGTCAACTTGTCCCCAGC -3'

Sequencing Primer
(F):5'- TTTACCTGAGCAAGAGGTCACAAAG -3'
(R):5'- GTTACTGTAGCTTAGAGAACCTGCC -3'

Posted On

2019-11-26