Mutagenetix > Incidental Mutation

Incidental Mutation 'R0959:Mug2'

81771

Institutional Source

Beutler Lab

Gene Symbol

Mug2

Ensembl Gene

ENSMUSG00000030131

Gene Name

murinoglobulin 2

Synonyms

MMRRC Submission

039088-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R0959 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

122006761-122085965 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 122085495 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Asparagine at position 1442 (S1442N)

Ref Sequence

ENSEMBL: ENSMUSP00000080469 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081777]

AlphaFold

P28666

Predicted Effect

probably benign
Transcript: ENSMUST00000081777
AA Change: S1442N

PolyPhen 2 Score 0.330 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000080469
Gene: ENSMUSG00000030131
AA Change: S1442N

Domain	Start	End	E-Value	Type
Pfam:A2M_N	128	221	3.5e-21	PFAM
A2M_N_2	449	599	1.05e-42	SMART
low complexity region	711	728	N/A	INTRINSIC
A2M	740	830	7.16e-36	SMART
Pfam:Thiol-ester_cl	963	992	1e-18	PFAM
low complexity region	994	1005	N/A	INTRINSIC
Pfam:A2M_comp	1012	1097	5.8e-34	PFAM
Pfam:A2M_comp	1093	1243	3e-47	PFAM
A2M_recep	1353	1440	1.85e-38	SMART

Coding Region Coverage

1x: 99.6%
3x: 98.7%
10x: 95.8%
20x: 89.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5830411N06Rik	A	G	7: 140,294,791	E394G	probably damaging	Het
Actl11	T	C	9: 107,931,235	V919A	probably damaging	Het
Adam28	G	A	14: 68,607,938	P761L	possibly damaging	Het
Aplf	G	A	6: 87,646,083	P338L	probably benign	Het
Arl5b	T	A	2: 15,073,131	I89N	probably damaging	Het
Asap2	T	C	12: 21,247,319	V596A	probably damaging	Het
Baz1b	T	G	5: 135,244,222	F1400C	probably damaging	Het
Ccdc149	A	T	5: 52,385,155	L365Q	probably damaging	Het
Ccdc60	A	G	5: 116,180,811	S149P	probably damaging	Het
Ces1b	A	G	8: 93,068,147	C275R	probably damaging	Het
Creb3	T	C	4: 43,563,509	L163P	probably damaging	Het
Dhx37	T	G	5: 125,423,432	N570T	probably benign	Het
Epc1	T	C	18: 6,453,657	N223D	probably damaging	Het
Gbp5	C	T	3: 142,503,124	H143Y	possibly damaging	Het
Gfod1	A	T	13: 43,303,429	D23E	probably benign	Het
Gm12169	A	T	11: 46,536,420	R187*	probably null	Het
Gm7361	A	T	5: 26,262,053	E223D	possibly damaging	Het
Izumo1	A	G	7: 45,624,991	K161E	probably damaging	Het
Kcnh2	C	T	5: 24,322,672	R894H	probably damaging	Het
Lrp1b	T	C	2: 41,268,354	N1617S	possibly damaging	Het
Med13l	A	T	5: 118,754,285	E1924D	possibly damaging	Het
Mroh2a	GCCC	GC	1: 88,232,257		probably null	Het
Mtg2	T	G	2: 180,083,428	S145A	probably benign	Het
Myo1b	A	T	1: 51,797,087	I315N	probably damaging	Het
Naip2	G	A	13: 100,154,878	T1184M	probably benign	Het
Naip2	T	A	13: 100,154,911	H1173L	probably benign	Het
Nsrp1	G	A	11: 77,046,459	R304*	probably null	Het
Olfr444	G	T	6: 42,955,752	V85L	probably benign	Het
Oxtr	C	T	6: 112,477,177	R42Q	probably benign	Het
Parp4	T	C	14: 56,648,119	F1552L	unknown	Het
Pigw	G	A	11: 84,878,207	H99Y	probably benign	Het
Ppp6r2	G	A	15: 89,274,176	M444I	possibly damaging	Het
Rchy1	A	G	5: 91,957,617	F82L	probably damaging	Het
Reln	A	C	5: 22,227,628	F125V	probably damaging	Het
Riok1	G	A	13: 38,057,173	E435K	probably damaging	Het
Rnf213	A	G	11: 119,452,581	R3590G	probably damaging	Het
Scrib	G	C	15: 76,051,461	P1249A	probably benign	Het
Shc4	C	T	2: 125,678,687		probably null	Het
Slc12a2	T	A	18: 57,904,378	I520N	probably damaging	Het
Slc26a5	A	G	5: 21,816,961	I484T	probably benign	Het
Slc39a11	G	T	11: 113,464,073	T110K	probably benign	Het
Snd1	T	A	6: 28,884,971	S774T	probably benign	Het
Spata31d1c	C	T	13: 65,036,315	P557L	probably damaging	Het
Svs1	T	A	6: 48,988,632	C525S	possibly damaging	Het
Tada1	A	G	1: 166,388,629	D133G	probably benign	Het
Tg	A	T	15: 66,708,010	T1555S	probably damaging	Het
Thra	A	G	11: 98,753,629	E15G	possibly damaging	Het
Ttn	T	A	2: 76,795,096	I15128F	probably damaging	Het
Ube2r2	A	G	4: 41,174,066	Y68C	probably damaging	Het
Uncx	A	T	5: 139,546,687	N169I	probably damaging	Het
Vmn2r100	A	G	17: 19,523,524	Y483C	possibly damaging	Het
Xkr4	T	C	1: 3,216,674	D431G	probably damaging	Het
Xylb	C	A	9: 119,380,025	A311E	possibly damaging	Het
Zbtb39	C	A	10: 127,743,062	H502N	probably damaging	Het
Zbtb39	C	G	10: 127,742,306	Q250E	probably benign	Het
Zfp82	A	G	7: 30,056,451	L402P	probably damaging	Het
Zpbp2	G	A	11: 98,557,625	R256Q	probably benign	Het

Other mutations in Mug2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00708:Mug2	APN	6	122047487	missense	possibly damaging	0.83
IGL00957:Mug2	APN	6	122040654	missense	probably damaging	0.99
IGL01314:Mug2	APN	6	122081279	missense	possibly damaging	0.62
IGL01338:Mug2	APN	6	122049628	splice site	probably benign
IGL01477:Mug2	APN	6	122081684	splice site	probably benign
IGL01926:Mug2	APN	6	122036104	splice site	probably benign
IGL02019:Mug2	APN	6	122047435	missense	probably benign	0.02
IGL02305:Mug2	APN	6	122036056	missense	probably benign
IGL02310:Mug2	APN	6	122059123	splice site	probably benign
IGL02484:Mug2	APN	6	122072753	missense	probably damaging	1.00
IGL02516:Mug2	APN	6	122070843	missense	probably damaging	1.00
IGL02531:Mug2	APN	6	122072771	missense	probably damaging	1.00
IGL02666:Mug2	APN	6	122081326	missense	probably damaging	1.00
IGL02936:Mug2	APN	6	122081387	critical splice donor site	probably null
R0114:Mug2	UTSW	6	122040648	missense	probably damaging	1.00
R0119:Mug2	UTSW	6	122036063	missense	probably benign	0.00
R0123:Mug2	UTSW	6	122074714	missense	possibly damaging	0.89
R0144:Mug2	UTSW	6	122071011	splice site	probably benign
R0225:Mug2	UTSW	6	122074714	missense	possibly damaging	0.89
R0514:Mug2	UTSW	6	122081599	missense	probably damaging	1.00
R0763:Mug2	UTSW	6	122075294	missense	probably benign
R1104:Mug2	UTSW	6	122059055	missense	probably benign
R1239:Mug2	UTSW	6	122081678	splice site	probably benign
R1318:Mug2	UTSW	6	122077402	missense	probably damaging	1.00
R1460:Mug2	UTSW	6	122040533	splice site	probably benign
R1706:Mug2	UTSW	6	122036232	splice site	probably benign
R1761:Mug2	UTSW	6	122074705	missense	probably benign	0.20
R1901:Mug2	UTSW	6	122071842	missense	probably benign	0.02
R1913:Mug2	UTSW	6	122070870	missense	probably damaging	1.00
R1943:Mug2	UTSW	6	122079639	missense	probably benign
R2054:Mug2	UTSW	6	122077492	missense	probably damaging	1.00
R2060:Mug2	UTSW	6	122079612	missense	probably benign
R2420:Mug2	UTSW	6	122083460	missense	probably damaging	1.00
R2432:Mug2	UTSW	6	122084376	missense	possibly damaging	0.93
R2916:Mug2	UTSW	6	122074724	splice site	probably null
R2918:Mug2	UTSW	6	122074724	splice site	probably null
R3423:Mug2	UTSW	6	122047506	splice site	probably benign
R3834:Mug2	UTSW	6	122049787	critical splice donor site	probably null
R3902:Mug2	UTSW	6	122075567	missense	probably damaging	1.00
R3941:Mug2	UTSW	6	122063563	missense	probably benign
R4227:Mug2	UTSW	6	122040732	missense	probably benign	0.10
R4284:Mug2	UTSW	6	122063673	missense	probably benign	0.00
R4287:Mug2	UTSW	6	122063673	missense	probably benign	0.00
R4377:Mug2	UTSW	6	122071007	critical splice donor site	probably null
R4419:Mug2	UTSW	6	122079630	missense	probably damaging	1.00
R4498:Mug2	UTSW	6	122082752	missense	probably damaging	0.99
R4566:Mug2	UTSW	6	122079638	missense	probably benign	0.00
R4690:Mug2	UTSW	6	122036296	missense	probably benign
R4732:Mug2	UTSW	6	122071872	missense	probably damaging	0.99
R4733:Mug2	UTSW	6	122071872	missense	probably damaging	0.99
R4741:Mug2	UTSW	6	122079613	missense	probably benign
R4888:Mug2	UTSW	6	122081195	missense	probably damaging	1.00
R5199:Mug2	UTSW	6	122040660	missense	probably benign
R5347:Mug2	UTSW	6	122081592	missense	probably damaging	1.00
R5457:Mug2	UTSW	6	122049729	nonsense	probably null
R5495:Mug2	UTSW	6	122079650	missense	probably damaging	0.96
R5509:Mug2	UTSW	6	122084381	missense	possibly damaging	0.84
R6006:Mug2	UTSW	6	122083500	missense	probably null	0.98
R6180:Mug2	UTSW	6	122079606	missense	probably benign	0.01
R6184:Mug2	UTSW	6	122037046	missense	probably benign
R6199:Mug2	UTSW	6	122047439	missense	probably benign	0.05
R6262:Mug2	UTSW	6	122075255	missense	probably damaging	1.00
R6416:Mug2	UTSW	6	122082754	missense	probably damaging	1.00
R6548:Mug2	UTSW	6	122047442	missense	probably damaging	1.00
R6703:Mug2	UTSW	6	122078694	missense	probably benign	0.25
R7106:Mug2	UTSW	6	122082721	missense	probably damaging	1.00
R7131:Mug2	UTSW	6	122075247	missense	probably damaging	1.00
R7372:Mug2	UTSW	6	122083466	missense	possibly damaging	0.88
R7379:Mug2	UTSW	6	122047487	missense	possibly damaging	0.83
R7419:Mug2	UTSW	6	122040570	missense	possibly damaging	0.86
R7423:Mug2	UTSW	6	122079726	missense	probably benign	0.00
R7581:Mug2	UTSW	6	122063711	missense	probably damaging	1.00
R7582:Mug2	UTSW	6	122079644	missense	probably damaging	0.99
R7672:Mug2	UTSW	6	122040719	missense	probably benign	0.37
R7713:Mug2	UTSW	6	122078795	missense	possibly damaging	0.83
R7759:Mug2	UTSW	6	122081358	missense	probably damaging	1.00
R7834:Mug2	UTSW	6	122036282	missense	probably benign
R7850:Mug2	UTSW	6	122075211	missense	probably damaging	1.00
R8029:Mug2	UTSW	6	122081545	critical splice acceptor site	probably null
R8127:Mug2	UTSW	6	122075608	missense	probably benign	0.01
R8335:Mug2	UTSW	6	122040584	missense	probably benign
R8348:Mug2	UTSW	6	122072233	nonsense	probably null
R8557:Mug2	UTSW	6	122063701	missense	probably damaging	0.99
R8798:Mug2	UTSW	6	122081610	missense	probably damaging	1.00
R8823:Mug2	UTSW	6	122063689	missense	possibly damaging	0.89
R9029:Mug2	UTSW	6	122084369	missense	probably damaging	1.00
R9153:Mug2	UTSW	6	122040668	missense	possibly damaging	0.71
R9185:Mug2	UTSW	6	122077483	missense	probably benign	0.06
R9186:Mug2	UTSW	6	122075289	missense	probably damaging	0.99
R9418:Mug2	UTSW	6	122040741	missense	probably benign	0.00
R9464:Mug2	UTSW	6	122051731	missense	probably benign	0.01
R9622:Mug2	UTSW	6	122051792	missense	probably benign	0.29
Z1177:Mug2	UTSW	6	122037121	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCACACTTGGATTTCTCCACAGGG -3'
(R):5'- ATCTACCTGGACACAGATAGCAGGG -3'

Sequencing Primer
(F):5'- atgtgttctctccttctgcc -3'
(R):5'- CTATACACTTTGGTAAGGAAGTTGG -3'

Posted On

2013-11-08