Mutagenetix > Incidental Mutations

Incidental Mutation 'R7255:Zfp160'

564230

Institutional Source

Beutler Lab

Gene Symbol

Zfp160

Ensembl Gene

ENSMUSG00000067942

Gene Name

zinc finger protein 160

Synonyms

6720480D16Rik, 6720480D16Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7255 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21008903-21043070 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 21025487 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 100 (S100T)

Ref Sequence

ENSEMBL: ENSMUSP00000086191 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088811] [ENSMUST00000231482] [ENSMUST00000232320] [ENSMUST00000232354] [ENSMUST00000232473] [ENSMUST00000232595] [ENSMUST00000232663]

AlphaFold

E9Q459

Predicted Effect

probably benign
Transcript: ENSMUST00000088811
AA Change: S100T

PolyPhen 2 Score 0.177 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000086191
Gene: ENSMUSG00000067942
AA Change: S100T

Domain	Start	End	E-Value	Type
KRAB	8	68	1.91e-29	SMART
low complexity region	100	110	N/A	INTRINSIC
ZnF_C2H2	146	168	1.69e-3	SMART
ZnF_C2H2	174	196	2.91e-2	SMART
ZnF_C2H2	202	224	1.4e-4	SMART
ZnF_C2H2	230	252	3.89e-3	SMART
ZnF_C2H2	258	280	1.72e-4	SMART
ZnF_C2H2	286	308	4.94e-5	SMART
ZnF_C2H2	314	336	2.12e-4	SMART
ZnF_C2H2	342	364	1.12e-3	SMART
ZnF_C2H2	370	392	1.2e-3	SMART
ZnF_C2H2	398	420	6.42e-4	SMART
ZnF_C2H2	426	448	9.08e-4	SMART
ZnF_C2H2	454	476	1.84e-4	SMART
ZnF_C2H2	482	504	1.5e-4	SMART
ZnF_C2H2	510	532	3.44e-4	SMART
ZnF_C2H2	538	560	1.12e-3	SMART
ZnF_C2H2	566	588	2.27e-4	SMART
ZnF_C2H2	594	616	1.04e-3	SMART
ZnF_C2H2	622	644	1.13e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000231482

Predicted Effect

probably benign
Transcript: ENSMUST00000232320

Predicted Effect

probably benign
Transcript: ENSMUST00000232354
AA Change: S100T

PolyPhen 2 Score 0.177 (Sensitivity: 0.92; Specificity: 0.87)

Predicted Effect

probably benign
Transcript: ENSMUST00000232473

Predicted Effect

probably benign
Transcript: ENSMUST00000232595

Predicted Effect

probably benign
Transcript: ENSMUST00000232663

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

97% (66/68)

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610010F05Rik	G	A	11: 23,620,465	P145L	probably benign	Het
9530003J23Rik	T	C	10: 117,234,422	H150R	probably benign	Het
Aldh1a7	A	G	19: 20,714,728	S234P	probably damaging	Het
Arhgap28	T	C	17: 67,853,004	H650R	probably damaging	Het
Asic1	A	G	15: 99,697,457	D355G	probably damaging	Het
Atp8b1	T	A	18: 64,556,868	S598C	probably damaging	Het
Bcat1	C	A	6: 145,032,785	E237*	probably null	Het
Btbd16	A	T	7: 130,785,992	I114F	probably benign	Het
Casp2	A	G	6: 42,268,907	D166G	probably damaging	Het
Cd86	CA	CAA	16: 36,606,555		probably null	Het
Cpsf1	G	A	15: 76,597,543	T1099M	probably damaging	Het
Crisp4	C	A	1: 18,130,231	A116S	probably damaging	Het
Cyb5r3	A	G	15: 83,160,165	I168T	probably damaging	Het
Dip2b	G	A	15: 100,209,627	D1407N	probably benign	Het
Dnajc8	A	G	4: 132,551,573	K201R	probably benign	Het
Dock10	C	T	1: 80,543,099		probably null	Het
Dopey2	C	A	16: 93,770,146	H1272N	probably damaging	Het
Dsc1	C	T	18: 20,097,273	R325Q	probably benign	Het
Enpp1	A	T	10: 24,645,315	I838K	possibly damaging	Het
Fcna	T	G	2: 25,626,028	D159A	probably damaging	Het
Flnc	G	T	6: 29,445,766	G840C	probably damaging	Het
Flt1	C	T	5: 147,580,406	A1024T	probably damaging	Het
Galc	T	C	12: 98,246,255	K207R	probably null	Het
Gbp2b	T	A	3: 142,608,117	L386Q	probably damaging	Het
Gm8297	T	A	14: 4,984,874	N48K	probably damaging	Het
Gm9639	G	A	10: 77,794,538	P180L	unknown	Het
Inpp5a	A	G	7: 139,511,448	N116S	probably damaging	Het
Ipo9	T	C	1: 135,385,988	E984G	probably benign	Het
Klra4	A	G	6: 130,059,642	F145L	probably damaging	Het
Lag3	A	G	6: 124,910,235	L123P	probably benign	Het
Med7	T	A	11: 46,440,995	M139K	probably damaging	Het
Mfsd2a	A	C	4: 122,952,021	L153R	possibly damaging	Het
Mup9	A	G	4: 60,421,337	V71A	probably benign	Het
Myo16	A	T	8: 10,499,169	Q927L	unknown	Het
Myo9b	T	C	8: 71,290,891	Y199H	probably damaging	Het
Nefm	A	G	14: 68,116,000	F406L	probably benign	Het
Olfr1123	T	A	2: 87,418,942	L296Q	probably damaging	Het
Olfr1333	A	T	4: 118,829,952	F162I	probably benign	Het
Pamr1	T	C	2: 102,611,584	F173L	probably damaging	Het
Pds5b	T	A	5: 150,796,667	D1205E	probably benign	Het
Plxna2	T	G	1: 194,752,103	F646V	probably benign	Het
Pnrc1	C	T	4: 33,248,045	G118D	probably benign	Het
Ppp1r16b	T	C	2: 158,761,391	F412S	probably benign	Het
Prcc	A	T	3: 87,870,091	V192E	probably damaging	Het
Psg19	A	G	7: 18,794,048	Y257H	probably benign	Het
Rfx7	T	G	9: 72,619,828	S1433R	possibly damaging	Het
Rgl2	T	C	17: 33,934,990	F457L	possibly damaging	Het
Sbspon	G	T	1: 15,883,797	C86*	probably null	Het
Sdhb	A	G	4: 140,977,418	E230G	possibly damaging	Het
Sema6b	T	C	17: 56,125,336	T581A	probably benign	Het
Shkbp1	G	T	7: 27,342,748	T594K	possibly damaging	Het
Shpk	A	G	11: 73,199,660	S48G	probably benign	Het
Slc1a3	A	G	15: 8,642,999	V332A	possibly damaging	Het
Slc25a25	T	C	2: 32,421,372	E135G	possibly damaging	Het
Slc5a8	A	T	10: 88,909,631	D367V	probably damaging	Het
Slco1c1	A	G	6: 141,569,325	T649A	probably benign	Het
Sorbs1	G	C	19: 40,376,800	R180G	probably benign	Het
Spats1	T	A	17: 45,454,205	D163V	probably damaging	Het
Ssh2	T	A	11: 77,425,593	M304K	probably damaging	Het
Sulf1	T	A	1: 12,859,008	D166E	probably benign	Het
Syne1	A	G	10: 5,333,446	S1540P	probably damaging	Het
Tcf7l1	A	T	6: 72,627,347		probably null	Het
Tet1	A	T	10: 62,822,636	M1477K	probably benign	Het
Tlr5	T	C	1: 182,974,316	F395S	probably damaging	Het
Trrap	T	C	5: 144,858,954	L3847P	probably damaging	Het
Tsks	C	T	7: 44,952,688	S276L	probably benign	Het
Uggt1	T	C	1: 36,146,106	E1519G	probably damaging	Het
Vps13a	A	G	19: 16,654,339		probably null	Het
Wdfy4	A	G	14: 32,974,282	V2768A		Het
Wdr26	A	C	1: 181,181,324	I627R	probably benign	Het

Other mutations in Zfp160

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00714:Zfp160	APN	17	21026702	missense	probably benign
IGL01019:Zfp160	APN	17	21020826	missense	possibly damaging	0.68
IGL02430:Zfp160	APN	17	21025530	missense	possibly damaging	0.76
R0412:Zfp160	UTSW	17	21026877	missense	probably damaging	0.97
R0600:Zfp160	UTSW	17	21027006	missense	probably benign	0.00
R2146:Zfp160	UTSW	17	21026982	missense	probably benign	0.13
R2157:Zfp160	UTSW	17	21020828	missense	probably benign	0.23
R2411:Zfp160	UTSW	17	21025745	missense	possibly damaging	0.94
R2904:Zfp160	UTSW	17	21025649	missense	probably benign	0.00
R4249:Zfp160	UTSW	17	21025738	missense	probably benign	0.11
R4896:Zfp160	UTSW	17	21020081	missense	probably benign	0.00
R5106:Zfp160	UTSW	17	21026761	missense	probably damaging	0.99
R5342:Zfp160	UTSW	17	21020733	missense	possibly damaging	0.95
R5352:Zfp160	UTSW	17	21026852	missense	probably benign	0.02
R6193:Zfp160	UTSW	17	21026862	missense	probably benign	0.24
R6230:Zfp160	UTSW	17	21026445	missense	probably benign	0.38
R6753:Zfp160	UTSW	17	21020734	missense	probably benign	0.02
R6928:Zfp160	UTSW	17	21041462	missense	probably benign	0.04
R7040:Zfp160	UTSW	17	21026532	missense	probably damaging	1.00
R7497:Zfp160	UTSW	17	21026193	missense	probably benign	0.08
R7510:Zfp160	UTSW	17	21026393	missense	probably benign	0.00
R7540:Zfp160	UTSW	17	21025660	nonsense	probably null
R7627:Zfp160	UTSW	17	21027008	missense	probably damaging	0.99
R8169:Zfp160	UTSW	17	21027036	missense	probably damaging	0.97
R8240:Zfp160	UTSW	17	21026088	missense	probably damaging	0.99
R8330:Zfp160	UTSW	17	21026051	missense	probably damaging	1.00
R8367:Zfp160	UTSW	17	21025542	missense	probably benign	0.22
R8802:Zfp160	UTSW	17	21026605	missense	probably damaging	1.00
R9183:Zfp160	UTSW	17	21020092	missense	possibly damaging	0.84
Z1177:Zfp160	UTSW	17	21026890	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCAGCAGAACATTCAGAATTTTGAG -3'
(R):5'- TTTCTCCAGTATGAATCTGCCG -3'

Sequencing Primer
(F):5'- GAATTTTGAGTTTCAGTGGAAAGAGC -3'
(R):5'- CCAGTATGAATCTGCCGATGATATGC -3'

Posted On

2019-06-26