Mutagenetix > Incidental Mutation

Incidental Mutation 'R1645:Lama2'

173825

Institutional Source

Beutler Lab

Gene Symbol

Lama2

Ensembl Gene

ENSMUSG00000019899

Gene Name

laminin, alpha 2

Synonyms

mer, merosin

MMRRC Submission

039681-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.320) question?

Stock #

R1645 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

26857281-27493021 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 27244981 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 267 (T267S)

Ref Sequence

ENSEMBL: ENSMUSP00000090304 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092639] [ENSMUST00000189575]

AlphaFold

no structure available at present

PDB Structure

LAMININ ALPHA 2 CHAIN LG4-5 DOMAIN PAIR [X-RAY DIFFRACTION]
LAMININ ALPHA 2 CHAIN LG4-5 DOMAIN PAIR, CA1 SITE MUTANT [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF THE FIFTH LAMININ G-LIKE MODULE OF THE MOUSE LAMININ ALPHA2 CHAIN [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF THE LG1-3 REGION OF THE LAMININ ALPHA2 CHAIN [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000092639
AA Change: T267S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000090304
Gene: ENSMUSG00000019899
AA Change: T267S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LamNT	29	281	5.35e-129	SMART
EGF_Lam	283	337	2.11e-4	SMART
EGF_Lam	340	407	1.59e-8	SMART
EGF_Lam	410	462	5.44e-7	SMART
EGF_Lam	465	511	9.05e-4	SMART
LamB	574	706	2.26e-44	SMART
Pfam:Laminin_EGF	715	745	2.8e-4	PFAM
EGF_Lam	753	800	4.03e-10	SMART
EGF_Lam	803	858	3.01e-9	SMART
EGF_Lam	861	911	1.35e-11	SMART
EGF_Lam	914	960	7.23e-12	SMART
EGF_Lam	963	1007	5.87e-12	SMART
EGF_Lam	1010	1053	1.28e-12	SMART
EGF_Lam	1056	1099	2.37e-7	SMART
EGF_Lam	1102	1159	3.22e-9	SMART
LamB	1225	1360	1.95e-57	SMART
EGF_like	1364	1413	8.13e-1	SMART
EGF_Lam	1416	1462	5.48e-12	SMART
EGF_Lam	1465	1520	1.27e-7	SMART
EGF_Lam	1523	1567	2.4e-8	SMART
Pfam:Laminin_I	1584	1849	2e-92	PFAM
Blast:MA	1881	2113	1e-112	BLAST
LamG	2162	2307	1.28e-25	SMART
LamG	2356	2500	2.2e-33	SMART
LamG	2542	2688	3.31e-28	SMART
low complexity region	2725	2741	N/A	INTRINSIC
LamG	2781	2914	2.25e-39	SMART
LamG	2956	3092	1.53e-32	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185839

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186965

Predicted Effect

probably damaging
Transcript: ENSMUST00000189575
AA Change: T267S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000140716
Gene: ENSMUSG00000019899
AA Change: T267S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LamNT	29	281	2.5e-131	SMART
EGF_Lam	283	337	1e-6	SMART
EGF_Lam	340	407	7.7e-11	SMART
EGF_Lam	410	462	2.6e-9	SMART
EGF_Lam	465	511	4.5e-6	SMART
LamB	574	706	1.4e-46	SMART
Pfam:Laminin_EGF	715	745	1.7e-2	PFAM
EGF_Lam	753	800	1.9e-12	SMART
EGF_Lam	803	858	1.4e-11	SMART
EGF_Lam	861	911	6.7e-14	SMART
EGF_Lam	914	960	3.6e-14	SMART
EGF_Lam	963	1007	2.9e-14	SMART
EGF_Lam	1010	1053	6.1e-15	SMART
EGF_Lam	1056	1099	1.1e-9	SMART
EGF_Lam	1102	1159	1.5e-11	SMART
LamB	1225	1349	1.4e-45	SMART

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.3%
20x: 89.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted and spontaneous mutations exhibit progressive growth retardation, ataxia, muscle atrophy and degeneration, infertility, and premature lethality. Muscle fiber degeneration is evident as early as the first week of life. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	A	G	3: 121,948,926 (GRCm39)	M1732V	probably benign	Het
Acvr1	A	T	2: 58,352,911 (GRCm39)	C350S	probably damaging	Het
Adgrl2	A	T	3: 148,571,244 (GRCm39)	V130D	probably damaging	Het
Anapc1	A	G	2: 128,500,166 (GRCm39)		probably null	Het
Asap1	A	T	15: 63,961,324 (GRCm39)	V1116E	probably damaging	Het
Bcas1	T	A	2: 170,229,087 (GRCm39)	D308V	probably damaging	Het
Brca1	G	A	11: 101,400,879 (GRCm39)	H1468Y	probably benign	Het
C2cd5	A	G	6: 142,995,852 (GRCm39)	C421R	probably damaging	Het
C4b	A	G	17: 34,959,571 (GRCm39)	S363P	probably damaging	Het
Camk2b	A	T	11: 5,922,719 (GRCm39)	C484S	probably damaging	Het
Cby2	C	A	14: 75,821,089 (GRCm39)	R212L	probably benign	Het
Ccny	T	C	18: 9,345,199 (GRCm39)	T192A	probably damaging	Het
Chl1	G	T	6: 103,660,141 (GRCm39)	A356S	probably benign	Het
Dst	T	A	1: 34,264,803 (GRCm39)	Y4850N	probably damaging	Het
Dyrk4	C	A	6: 126,871,756 (GRCm39)	E171*	probably null	Het
Entrep3	C	A	3: 89,094,154 (GRCm39)	D322E	possibly damaging	Het
Ephb1	A	G	9: 101,804,758 (GRCm39)	Y928H	probably damaging	Het
Fam114a2	A	T	11: 57,390,621 (GRCm39)	N304K	probably benign	Het
Fras1	A	T	5: 96,848,445 (GRCm39)	D1820V	possibly damaging	Het
Gabbr2	A	T	4: 46,664,963 (GRCm39)		probably null	Het
Gm6358	T	C	16: 88,937,967 (GRCm39)	W69R	unknown	Het
Ikbkb	C	A	8: 23,181,082 (GRCm39)	S127I	probably damaging	Het
Impa1	A	T	3: 10,393,501 (GRCm39)	M48K	possibly damaging	Het
Klra2	A	T	6: 131,220,857 (GRCm39)		probably null	Het
Lama1	A	T	17: 68,044,677 (GRCm39)	Y192F	probably benign	Het
Mroh7	G	A	4: 106,577,865 (GRCm39)	T271I	probably benign	Het
Myo9b	A	G	8: 71,775,622 (GRCm39)	E348G	probably damaging	Het
Nrp2	C	T	1: 62,824,283 (GRCm39)	P796L	probably damaging	Het
Ntn4	C	T	10: 93,543,215 (GRCm39)	R314W	probably damaging	Het
Or10al4	A	T	17: 38,037,229 (GRCm39)	T114S	probably benign	Het
Or51i1	A	G	7: 103,671,210 (GRCm39)	F105S	probably damaging	Het
Or9k7	T	A	10: 130,046,081 (GRCm39)	D306V	probably damaging	Het
P3h2	T	A	16: 25,815,982 (GRCm39)	H177L	probably damaging	Het
Pcdhb16	T	C	18: 37,612,423 (GRCm39)	I461T	probably benign	Het
Pdlim3	A	G	8: 46,349,785 (GRCm39)	I32V	probably benign	Het
Pigu	A	C	2: 155,170,598 (GRCm39)	Y143*	probably null	Het
Prkd3	A	C	17: 79,263,949 (GRCm39)		probably null	Het
Psrc1	C	T	3: 108,292,554 (GRCm39)	R116W	probably damaging	Het
Rab5b	A	G	10: 128,522,695 (GRCm39)	S29P	possibly damaging	Het
Rbm26	A	G	14: 105,388,253 (GRCm39)	V403A	probably damaging	Het
Rbm47	G	T	5: 66,184,481 (GRCm39)	R41S	probably benign	Het
Rngtt	A	G	4: 33,362,939 (GRCm39)	I364M	probably damaging	Het
Ryr2	A	T	13: 11,733,368 (GRCm39)	C2271*	probably null	Het
Shcbp1	T	A	8: 4,799,645 (GRCm39)	Q277L	probably benign	Het
Sntg1	T	C	1: 8,874,155 (GRCm39)	T5A	probably benign	Het
Snx24	T	C	18: 53,522,634 (GRCm39)	F163S	probably benign	Het
Srp72	T	C	5: 77,146,125 (GRCm39)	V581A	probably benign	Het
Srrt	T	A	5: 137,300,401 (GRCm39)	K59*	probably null	Het
Tnrc6b	A	G	15: 80,767,159 (GRCm39)	T975A	probably damaging	Het
Vmn1r174	G	A	7: 23,453,777 (GRCm39)	V148I	possibly damaging	Het
Vmn2r115	T	A	17: 23,565,192 (GRCm39)	C360S	possibly damaging	Het
Vwa8	A	T	14: 79,420,427 (GRCm39)	Q1709H	probably damaging	Het
Wdr11	A	G	7: 129,215,613 (GRCm39)	T526A	probably benign	Het
Zfp532	C	A	18: 65,820,335 (GRCm39)	N973K	probably benign	Het

Other mutations in Lama2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00392:Lama2	APN	10	27,064,261 (GRCm39)	missense	probably benign	0.01
IGL00467:Lama2	APN	10	27,343,193 (GRCm39)	splice site	probably benign
IGL00470:Lama2	APN	10	27,119,738 (GRCm39)	missense	probably benign	0.22
IGL00517:Lama2	APN	10	27,073,326 (GRCm39)	missense	probably benign	0.01
IGL00541:Lama2	APN	10	27,064,302 (GRCm39)	missense	probably benign	0.14
IGL00931:Lama2	APN	10	26,882,772 (GRCm39)	missense	possibly damaging	0.92
IGL00951:Lama2	APN	10	26,906,281 (GRCm39)	missense	probably benign	0.03
IGL00988:Lama2	APN	10	27,245,011 (GRCm39)	nonsense	probably null
IGL01098:Lama2	APN	10	26,907,108 (GRCm39)	missense	possibly damaging	0.66
IGL01152:Lama2	APN	10	27,084,425 (GRCm39)	missense	probably benign	0.00
IGL01293:Lama2	APN	10	27,107,632 (GRCm39)	missense	probably benign	0.38
IGL01338:Lama2	APN	10	27,064,268 (GRCm39)	missense	probably benign	0.13
IGL01609:Lama2	APN	10	27,220,417 (GRCm39)	missense	probably benign	0.03
IGL01643:Lama2	APN	10	26,946,368 (GRCm39)	splice site	probably benign
IGL01675:Lama2	APN	10	27,064,050 (GRCm39)	missense	possibly damaging	0.77
IGL01681:Lama2	APN	10	27,141,041 (GRCm39)	missense	probably benign	0.33
IGL01694:Lama2	APN	10	26,882,738 (GRCm39)	missense	possibly damaging	0.75
IGL01705:Lama2	APN	10	27,065,270 (GRCm39)	splice site	probably benign
IGL01885:Lama2	APN	10	26,981,135 (GRCm39)	nonsense	probably null
IGL01935:Lama2	APN	10	27,298,600 (GRCm39)	missense	probably damaging	0.98
IGL01994:Lama2	APN	10	27,343,199 (GRCm39)	critical splice donor site	probably null
IGL02041:Lama2	APN	10	26,860,322 (GRCm39)	missense	probably damaging	1.00
IGL02067:Lama2	APN	10	27,052,792 (GRCm39)	missense	probably benign	0.02
IGL02097:Lama2	APN	10	27,014,956 (GRCm39)	missense	probably benign	0.09
IGL02179:Lama2	APN	10	26,946,360 (GRCm39)	missense	probably benign	0.01
IGL02268:Lama2	APN	10	26,877,112 (GRCm39)	splice site	probably benign
IGL02302:Lama2	APN	10	27,088,039 (GRCm39)	missense	probably benign	0.06
IGL02363:Lama2	APN	10	27,242,062 (GRCm39)	missense	probably damaging	1.00
IGL02378:Lama2	APN	10	26,919,652 (GRCm39)	missense	probably damaging	0.99
IGL02642:Lama2	APN	10	27,343,269 (GRCm39)	missense	probably damaging	1.00
IGL02676:Lama2	APN	10	26,994,489 (GRCm39)	missense	probably benign	0.00
IGL02695:Lama2	APN	10	26,876,771 (GRCm39)	missense	probably benign
IGL02735:Lama2	APN	10	26,980,124 (GRCm39)	missense	probably damaging	1.00
IGL02794:Lama2	APN	10	26,917,227 (GRCm39)	missense	possibly damaging	0.73
IGL02823:Lama2	APN	10	26,877,141 (GRCm39)	missense	probably damaging	1.00
IGL02869:Lama2	APN	10	26,891,534 (GRCm39)	missense	probably damaging	0.99
IGL02942:Lama2	APN	10	26,917,216 (GRCm39)	missense	probably damaging	1.00
IGL03201:Lama2	APN	10	27,220,566 (GRCm39)	nonsense	probably null
IGL03268:Lama2	APN	10	27,298,649 (GRCm39)	missense	probably damaging	1.00
IGL03288:Lama2	APN	10	27,245,047 (GRCm39)	missense	probably damaging	1.00
IGL03380:Lama2	APN	10	26,926,261 (GRCm39)	missense	probably damaging	1.00
IGL03407:Lama2	APN	10	27,223,017 (GRCm39)	missense	probably damaging	1.00
cowboy	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
petri	UTSW	10	26,869,394 (GRCm39)	splice site	probably null
PIT4362001:Lama2	UTSW	10	27,245,132 (GRCm39)	missense	probably damaging	1.00
PIT4382001:Lama2	UTSW	10	27,080,901 (GRCm39)	missense	probably damaging	1.00
PIT4431001:Lama2	UTSW	10	26,977,426 (GRCm39)	missense	probably damaging	1.00
R0038:Lama2	UTSW	10	26,862,793 (GRCm39)	missense	probably benign	0.02
R0038:Lama2	UTSW	10	26,862,793 (GRCm39)	missense	probably benign	0.02
R0114:Lama2	UTSW	10	26,869,064 (GRCm39)	nonsense	probably null
R0142:Lama2	UTSW	10	27,063,841 (GRCm39)	missense	probably benign
R0313:Lama2	UTSW	10	26,869,394 (GRCm39)	splice site	probably null
R0376:Lama2	UTSW	10	26,891,542 (GRCm39)	missense	possibly damaging	0.68
R0412:Lama2	UTSW	10	27,066,621 (GRCm39)	missense	possibly damaging	0.58
R0472:Lama2	UTSW	10	26,866,863 (GRCm39)	missense	probably damaging	1.00
R0607:Lama2	UTSW	10	27,065,127 (GRCm39)	missense	probably benign	0.34
R0648:Lama2	UTSW	10	26,865,372 (GRCm39)	missense	probably benign	0.00
R0667:Lama2	UTSW	10	27,220,406 (GRCm39)	splice site	probably null
R0760:Lama2	UTSW	10	26,920,429 (GRCm39)	critical splice donor site	probably null
R1240:Lama2	UTSW	10	26,917,120 (GRCm39)	missense	probably damaging	1.00
R1385:Lama2	UTSW	10	27,100,039 (GRCm39)	missense	probably benign	0.11
R1433:Lama2	UTSW	10	27,063,750 (GRCm39)	missense	probably damaging	1.00
R1434:Lama2	UTSW	10	27,084,366 (GRCm39)	missense	probably damaging	1.00
R1574:Lama2	UTSW	10	27,200,750 (GRCm39)	missense	possibly damaging	0.65
R1574:Lama2	UTSW	10	27,200,750 (GRCm39)	missense	possibly damaging	0.65
R1702:Lama2	UTSW	10	27,066,525 (GRCm39)	missense	probably benign
R1703:Lama2	UTSW	10	27,142,667 (GRCm39)	missense	probably damaging	1.00
R1769:Lama2	UTSW	10	27,084,403 (GRCm39)	missense	probably benign
R1769:Lama2	UTSW	10	27,084,402 (GRCm39)	missense	probably damaging	1.00
R1846:Lama2	UTSW	10	27,088,092 (GRCm39)	missense	probably damaging	1.00
R1859:Lama2	UTSW	10	26,907,078 (GRCm39)	missense	possibly damaging	0.51
R1871:Lama2	UTSW	10	26,860,490 (GRCm39)	missense	probably damaging	1.00
R1903:Lama2	UTSW	10	27,064,395 (GRCm39)	missense	probably damaging	1.00
R1906:Lama2	UTSW	10	26,932,523 (GRCm39)	critical splice donor site	probably null
R1958:Lama2	UTSW	10	26,857,594 (GRCm39)	missense	probably damaging	0.97
R1959:Lama2	UTSW	10	27,298,614 (GRCm39)	missense	probably damaging	1.00
R1977:Lama2	UTSW	10	26,866,796 (GRCm39)	splice site	probably null
R2063:Lama2	UTSW	10	27,040,922 (GRCm39)	missense	probably damaging	1.00
R2079:Lama2	UTSW	10	27,245,049 (GRCm39)	missense	probably damaging	0.99
R2085:Lama2	UTSW	10	27,080,837 (GRCm39)	nonsense	probably null
R2125:Lama2	UTSW	10	26,920,449 (GRCm39)	nonsense	probably null
R2140:Lama2	UTSW	10	26,930,690 (GRCm39)	splice site	probably null
R2219:Lama2	UTSW	10	26,919,565 (GRCm39)	missense	probably damaging	0.99
R2259:Lama2	UTSW	10	26,907,123 (GRCm39)	missense	probably benign	0.00
R2265:Lama2	UTSW	10	26,868,932 (GRCm39)	missense	probably damaging	1.00
R2266:Lama2	UTSW	10	26,862,793 (GRCm39)	missense	probably benign	0.02
R2267:Lama2	UTSW	10	26,868,932 (GRCm39)	missense	probably damaging	1.00
R2268:Lama2	UTSW	10	26,868,932 (GRCm39)	missense	probably damaging	1.00
R2269:Lama2	UTSW	10	26,868,932 (GRCm39)	missense	probably damaging	1.00
R2862:Lama2	UTSW	10	27,298,608 (GRCm39)	nonsense	probably null
R2912:Lama2	UTSW	10	26,876,799 (GRCm39)	missense	probably benign
R2999:Lama2	UTSW	10	26,865,417 (GRCm39)	missense	probably benign	0.18
R3034:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3081:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3107:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3109:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3436:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3437:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3706:Lama2	UTSW	10	27,014,992 (GRCm39)	missense	probably damaging	1.00
R3780:Lama2	UTSW	10	27,335,335 (GRCm39)	missense	probably damaging	1.00
R3807:Lama2	UTSW	10	27,066,661 (GRCm39)	frame shift	probably null
R3919:Lama2	UTSW	10	26,994,501 (GRCm39)	missense	probably damaging	1.00
R4014:Lama2	UTSW	10	26,860,372 (GRCm39)	missense	probably damaging	1.00
R4131:Lama2	UTSW	10	26,917,170 (GRCm39)	missense	probably benign	0.00
R4190:Lama2	UTSW	10	27,142,660 (GRCm39)	missense	probably damaging	0.96
R4273:Lama2	UTSW	10	27,223,050 (GRCm39)	missense	probably damaging	1.00
R4358:Lama2	UTSW	10	26,860,489 (GRCm39)	missense	probably damaging	1.00
R4407:Lama2	UTSW	10	27,088,124 (GRCm39)	small deletion	probably benign
R4415:Lama2	UTSW	10	26,865,340 (GRCm39)	nonsense	probably null
R4426:Lama2	UTSW	10	27,298,554 (GRCm39)	missense	probably damaging	1.00
R4590:Lama2	UTSW	10	26,865,410 (GRCm39)	missense	probably benign	0.00
R4615:Lama2	UTSW	10	26,857,520 (GRCm39)	missense	probably damaging	0.99
R4736:Lama2	UTSW	10	27,080,925 (GRCm39)	missense	probably damaging	1.00
R4754:Lama2	UTSW	10	26,994,527 (GRCm39)	missense	possibly damaging	0.58
R4791:Lama2	UTSW	10	27,343,267 (GRCm39)	missense	probably damaging	1.00
R4834:Lama2	UTSW	10	26,882,745 (GRCm39)	missense	probably benign	0.30
R4856:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4858:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4859:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4897:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4898:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4899:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4907:Lama2	UTSW	10	27,040,942 (GRCm39)	missense	probably benign	0.11
R4911:Lama2	UTSW	10	27,014,923 (GRCm39)	missense	probably damaging	1.00
R4924:Lama2	UTSW	10	27,245,137 (GRCm39)	missense	probably damaging	0.98
R5023:Lama2	UTSW	10	27,066,500 (GRCm39)	missense	probably damaging	0.97
R5057:Lama2	UTSW	10	27,040,982 (GRCm39)	missense	probably damaging	1.00
R5070:Lama2	UTSW	10	27,226,247 (GRCm39)	critical splice donor site	probably null
R5116:Lama2	UTSW	10	26,994,556 (GRCm39)	missense	probably benign	0.08
R5177:Lama2	UTSW	10	27,066,699 (GRCm39)	missense	possibly damaging	0.94
R5198:Lama2	UTSW	10	27,222,999 (GRCm39)	missense	probably damaging	0.96
R5289:Lama2	UTSW	10	27,088,069 (GRCm39)	nonsense	probably null
R5327:Lama2	UTSW	10	27,014,942 (GRCm39)	missense	probably benign
R5424:Lama2	UTSW	10	26,860,392 (GRCm39)	missense	probably damaging	1.00
R5469:Lama2	UTSW	10	26,917,185 (GRCm39)	missense	possibly damaging	0.92
R5620:Lama2	UTSW	10	26,866,876 (GRCm39)	missense	probably damaging	0.99
R5667:Lama2	UTSW	10	27,066,540 (GRCm39)	missense	probably damaging	1.00
R5671:Lama2	UTSW	10	27,066,540 (GRCm39)	missense	probably damaging	1.00
R5815:Lama2	UTSW	10	26,862,847 (GRCm39)	missense	probably damaging	1.00
R5917:Lama2	UTSW	10	27,066,693 (GRCm39)	missense	probably damaging	1.00
R5935:Lama2	UTSW	10	26,891,494 (GRCm39)	missense	probably benign
R5976:Lama2	UTSW	10	27,066,672 (GRCm39)	missense	probably benign	0.00
R5979:Lama2	UTSW	10	27,111,728 (GRCm39)	missense	probably damaging	0.99
R6004:Lama2	UTSW	10	27,111,781 (GRCm39)	missense	probably benign	0.01
R6180:Lama2	UTSW	10	26,857,495 (GRCm39)	missense	probably benign	0.03
R6198:Lama2	UTSW	10	27,064,018 (GRCm39)	missense	probably damaging	1.00
R6257:Lama2	UTSW	10	26,862,895 (GRCm39)	missense	possibly damaging	0.85
R6271:Lama2	UTSW	10	26,899,325 (GRCm39)	missense	possibly damaging	0.67
R6322:Lama2	UTSW	10	27,066,543 (GRCm39)	missense	probably damaging	0.96
R6354:Lama2	UTSW	10	27,088,064 (GRCm39)	missense	probably damaging	1.00
R6431:Lama2	UTSW	10	26,929,027 (GRCm39)	missense	possibly damaging	0.50
R6499:Lama2	UTSW	10	26,907,154 (GRCm39)	missense	probably damaging	1.00
R6535:Lama2	UTSW	10	26,980,127 (GRCm39)	missense	probably damaging	1.00
R6545:Lama2	UTSW	10	27,052,793 (GRCm39)	missense	probably benign
R6636:Lama2	UTSW	10	27,000,564 (GRCm39)	missense	probably benign	0.13
R6891:Lama2	UTSW	10	27,204,078 (GRCm39)	nonsense	probably null
R6891:Lama2	UTSW	10	27,204,068 (GRCm39)	nonsense	probably null
R6902:Lama2	UTSW	10	26,857,625 (GRCm39)	missense	probably damaging	1.00
R6908:Lama2	UTSW	10	26,907,192 (GRCm39)	splice site	probably null
R7168:Lama2	UTSW	10	27,242,148 (GRCm39)	critical splice acceptor site	probably null
R7233:Lama2	UTSW	10	27,107,659 (GRCm39)	missense	probably damaging	1.00
R7272:Lama2	UTSW	10	27,000,552 (GRCm39)	missense	probably damaging	1.00
R7274:Lama2	UTSW	10	26,995,976 (GRCm39)	missense	probably damaging	0.99
R7419:Lama2	UTSW	10	27,142,630 (GRCm39)	missense	probably benign
R7423:Lama2	UTSW	10	27,088,222 (GRCm39)	missense	probably benign	0.00
R7554:Lama2	UTSW	10	27,031,492 (GRCm39)	missense	probably damaging	1.00
R7569:Lama2	UTSW	10	27,141,046 (GRCm39)	missense	probably damaging	1.00
R7574:Lama2	UTSW	10	26,882,726 (GRCm39)	missense	probably benign	0.03
R7584:Lama2	UTSW	10	26,980,257 (GRCm39)	missense	possibly damaging	0.78
R7586:Lama2	UTSW	10	26,977,389 (GRCm39)	missense	probably benign	0.00
R7603:Lama2	UTSW	10	27,142,676 (GRCm39)	missense	possibly damaging	0.55
R7691:Lama2	UTSW	10	27,084,389 (GRCm39)	missense	possibly damaging	0.67
R7750:Lama2	UTSW	10	26,866,920 (GRCm39)	missense	probably damaging	0.97
R7841:Lama2	UTSW	10	27,031,529 (GRCm39)	missense	probably benign	0.00
R7864:Lama2	UTSW	10	26,932,611 (GRCm39)	missense	probably benign	0.08
R7960:Lama2	UTSW	10	26,869,094 (GRCm39)	missense	probably benign	0.04
R7964:Lama2	UTSW	10	27,099,977 (GRCm39)	critical splice donor site	probably null
R7980:Lama2	UTSW	10	27,239,609 (GRCm39)	missense	probably damaging	0.98
R8013:Lama2	UTSW	10	27,220,494 (GRCm39)	missense	probably benign	0.00
R8028:Lama2	UTSW	10	27,204,145 (GRCm39)	missense	probably benign	0.13
R8097:Lama2	UTSW	10	27,066,660 (GRCm39)	nonsense	probably null
R8100:Lama2	UTSW	10	26,917,113 (GRCm39)	missense	probably benign	0.03
R8110:Lama2	UTSW	10	26,866,866 (GRCm39)	missense	probably damaging	1.00
R8122:Lama2	UTSW	10	26,930,592 (GRCm39)	missense	possibly damaging	0.87
R8264:Lama2	UTSW	10	27,343,218 (GRCm39)	missense	probably benign	0.07
R8315:Lama2	UTSW	10	27,298,655 (GRCm39)	missense	probably damaging	1.00
R8318:Lama2	UTSW	10	26,860,334 (GRCm39)	missense	probably damaging	1.00
R8419:Lama2	UTSW	10	27,298,559 (GRCm39)	missense	probably benign	0.26
R8475:Lama2	UTSW	10	26,977,369 (GRCm39)	missense	possibly damaging	0.69
R8735:Lama2	UTSW	10	27,066,530 (GRCm39)	missense	probably damaging	1.00
R8754:Lama2	UTSW	10	26,877,147 (GRCm39)	missense	possibly damaging	0.83
R8817:Lama2	UTSW	10	27,063,869 (GRCm39)	missense	probably damaging	1.00
R8851:Lama2	UTSW	10	27,242,119 (GRCm39)	missense	possibly damaging	0.94
R8859:Lama2	UTSW	10	27,335,384 (GRCm39)	missense	possibly damaging	0.58
R8886:Lama2	UTSW	10	27,245,157 (GRCm39)	splice site	probably benign
R8937:Lama2	UTSW	10	26,862,816 (GRCm39)	missense	probably damaging	1.00
R8993:Lama2	UTSW	10	27,298,710 (GRCm39)	missense	possibly damaging	0.91
R9025:Lama2	UTSW	10	26,860,367 (GRCm39)	missense	probably benign	0.00
R9027:Lama2	UTSW	10	27,080,881 (GRCm39)	missense	probably damaging	1.00
R9047:Lama2	UTSW	10	26,882,697 (GRCm39)	missense	possibly damaging	0.50
R9075:Lama2	UTSW	10	26,857,588 (GRCm39)	missense	probably damaging	1.00
R9135:Lama2	UTSW	10	27,298,515 (GRCm39)	missense	probably damaging	1.00
R9165:Lama2	UTSW	10	26,929,022 (GRCm39)	critical splice donor site	probably null
R9192:Lama2	UTSW	10	27,204,181 (GRCm39)	missense	possibly damaging	0.95
R9254:Lama2	UTSW	10	27,298,685 (GRCm39)	missense	probably damaging	0.96
R9326:Lama2	UTSW	10	26,906,193 (GRCm39)	missense	probably benign	0.04
R9356:Lama2	UTSW	10	27,088,186 (GRCm39)	missense	probably damaging	0.99
R9358:Lama2	UTSW	10	27,492,761 (GRCm39)	missense	unknown
R9358:Lama2	UTSW	10	27,064,378 (GRCm39)	missense	possibly damaging	0.95
R9376:Lama2	UTSW	10	26,994,620 (GRCm39)	missense	probably benign	0.11
R9381:Lama2	UTSW	10	27,064,023 (GRCm39)	nonsense	probably null
R9397:Lama2	UTSW	10	26,981,117 (GRCm39)	missense	probably benign	0.01
R9460:Lama2	UTSW	10	27,298,475 (GRCm39)	missense	probably damaging	1.00
R9478:Lama2	UTSW	10	26,891,478 (GRCm39)	missense	probably damaging	0.98
R9503:Lama2	UTSW	10	26,865,440 (GRCm39)	missense	possibly damaging	0.57
R9514:Lama2	UTSW	10	27,100,015 (GRCm39)	missense	probably benign	0.00
R9515:Lama2	UTSW	10	26,877,170 (GRCm39)	missense	probably benign	0.23
R9516:Lama2	UTSW	10	27,100,015 (GRCm39)	missense	probably benign	0.00
R9533:Lama2	UTSW	10	26,862,871 (GRCm39)	missense	probably damaging	1.00
R9619:Lama2	UTSW	10	27,064,282 (GRCm39)	missense	probably damaging	1.00
R9721:Lama2	UTSW	10	27,343,338 (GRCm39)	missense	possibly damaging	0.58

Predicted Primers

PCR Primer
(F):5'- TGGTACTGAAATAAAGCACGCCCTTAGA -3'
(R):5'- GGAGACCAAGTGCTGATGACCCCT -3'

Sequencing Primer
(F):5'- tcaacctcaatcctgctctatac -3'
(R):5'- TGATGACCCCTCCCCTGAAC -3'

Posted On

2014-04-24