Mutagenetix > Incidental Mutation

Incidental Mutation 'R7560:Hectd4'

585014

Institutional Source

Beutler Lab

Gene Symbol

Hectd4

Ensembl Gene

ENSMUSG00000042744

Gene Name

HECT domain E3 ubiquitin protein ligase 4

Synonyms

Gm15800

MMRRC Submission

045626-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.927) question?

Stock #

R7560 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

121358282-121506640 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 121392405 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 223 (N223S)

Ref Sequence

ENSEMBL: ENSMUSP00000048345 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042614]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000042614
AA Change: N223S

PolyPhen 2 Score 0.528 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000048345
Gene: ENSMUSG00000042744
AA Change: N223S

Domain	Start	End	E-Value	Type
low complexity region	224	234	N/A	INTRINSIC
low complexity region	266	282	N/A	INTRINSIC
low complexity region	553	564	N/A	INTRINSIC
low complexity region	725	735	N/A	INTRINSIC
low complexity region	1252	1265	N/A	INTRINSIC
coiled coil region	1372	1398	N/A	INTRINSIC
low complexity region	1551	1562	N/A	INTRINSIC
low complexity region	1725	1741	N/A	INTRINSIC
low complexity region	1892	1904	N/A	INTRINSIC
low complexity region	2656	2666	N/A	INTRINSIC
low complexity region	2857	2872	N/A	INTRINSIC
low complexity region	2901	2917	N/A	INTRINSIC
low complexity region	2921	2933	N/A	INTRINSIC
low complexity region	3232	3246	N/A	INTRINSIC
low complexity region	3275	3335	N/A	INTRINSIC
low complexity region	3441	3448	N/A	INTRINSIC
low complexity region	3473	3506	N/A	INTRINSIC
low complexity region	3512	3533	N/A	INTRINSIC
low complexity region	3540	3554	N/A	INTRINSIC
low complexity region	3794	3822	N/A	INTRINSIC
HECTc	4048	4412	4.78e-11	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (100/100)

Allele List at MGI

Other mutations in this stock

Total: 101 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot12	A	G	13: 91,932,510 (GRCm39)	D502G	probably benign	Het
Adrb1	T	C	19: 56,711,120 (GRCm39)	V106A	probably damaging	Het
Ahcyl2	G	T	6: 29,886,139 (GRCm39)	G352W	probably damaging	Het
Ahnak2	T	G	12: 112,745,851 (GRCm39)	D446A		Het
Arhgap42	T	C	9: 9,035,532 (GRCm39)	D270G	probably benign	Het
Atp2a1	A	G	7: 126,058,828 (GRCm39)	V129A	possibly damaging	Het
B9d2	G	A	7: 25,380,784 (GRCm39)		probably benign	Het
Bace1	A	T	9: 45,767,437 (GRCm39)	I207F	possibly damaging	Het
Blnk	T	A	19: 40,940,834 (GRCm39)	I190F	possibly damaging	Het
Bmp7	C	T	2: 172,781,757 (GRCm39)	E36K	possibly damaging	Het
Brsk2	A	G	7: 141,554,597 (GRCm39)	E654G	probably benign	Het
Cacna2d3	T	C	14: 28,780,378 (GRCm39)	H661R	probably benign	Het
Cdh4	T	C	2: 179,532,695 (GRCm39)	V719A	probably benign	Het
Cdkn2d	G	T	9: 21,200,540 (GRCm39)	P77H	probably damaging	Het
Chd3	A	G	11: 69,247,096 (GRCm39)	V1024A	probably damaging	Het
Chsy1	T	A	7: 65,820,992 (GRCm39)	M409K	possibly damaging	Het
Chsy1	T	C	7: 65,821,319 (GRCm39)	L518P	probably damaging	Het
Cic	G	A	7: 24,972,278 (GRCm39)	V670M	probably damaging	Het
Clock	A	T	5: 76,390,738 (GRCm39)		probably null	Het
Coro1a	C	A	7: 126,302,306 (GRCm39)	V14L	probably damaging	Het
Ctcfl	C	A	2: 172,960,199 (GRCm39)	C128F	probably damaging	Het
Cyb5b	A	G	8: 107,896,491 (GRCm39)	T71A	probably damaging	Het
Cyp2c40	C	G	19: 39,795,658 (GRCm39)	V174L	possibly damaging	Het
D630045J12Rik	T	A	6: 38,173,562 (GRCm39)	Q202L	possibly damaging	Het
Dgkz	T	C	2: 91,773,160 (GRCm39)		probably benign	Het
Dlgap2	T	C	8: 14,872,697 (GRCm39)		probably null	Het
Dnah1	T	C	14: 31,026,940 (GRCm39)	I695V	probably benign	Het
Dst	T	A	1: 34,221,532 (GRCm39)	S2445R	possibly damaging	Het
Elob	T	C	17: 24,043,950 (GRCm39)	D83G	probably benign	Het
Elp4	T	A	2: 105,624,933 (GRCm39)	D320V	probably damaging	Het
Enpp5	G	A	17: 44,396,155 (GRCm39)	G356S	probably damaging	Het
Etfdh	T	C	3: 79,530,886 (GRCm39)	Y45C	probably damaging	Het
Exph5	A	G	9: 53,287,073 (GRCm39)	I1385V	probably benign	Het
Fat3	A	T	9: 15,908,138 (GRCm39)	D2621E	probably damaging	Het
Fbxl6	G	T	15: 76,422,669 (GRCm39)	A83D	probably benign	Het
Fmo2	A	G	1: 162,716,318 (GRCm39)	Y90H	probably damaging	Het
Fzd4	T	A	7: 89,056,761 (GRCm39)	Y269*	probably null	Het
Grik5	T	A	7: 24,757,951 (GRCm39)	I377F	probably damaging	Het
Havcr2	C	A	11: 46,349,889 (GRCm39)	P137Q	probably damaging	Het
Hmcn2	A	G	2: 31,347,185 (GRCm39)	S4792G	probably benign	Het
Hps3	T	G	3: 20,084,616 (GRCm39)	I191L	probably benign	Het
Ica1l	T	A	1: 60,049,369 (GRCm39)	K195*	probably null	Het
Idh2	TCCCAGGGCC	TCC	7: 79,748,079 (GRCm39)		probably null	Het
Ift140	A	G	17: 25,311,315 (GRCm39)	D1170G	probably benign	Het
Igflr1	T	C	7: 30,266,776 (GRCm39)	F208S	possibly damaging	Het
Kcnma1	T	C	14: 23,580,310 (GRCm39)	T313A	probably benign	Het
Krt6a	T	A	15: 101,598,994 (GRCm39)	I524F	unknown	Het
Lce1d	G	T	3: 92,593,148 (GRCm39)	H88Q	unknown	Het
Lrmda	A	G	14: 22,878,770 (GRCm39)	E177G	probably benign	Het
Lrrn3	T	A	12: 41,502,712 (GRCm39)	N535I	possibly damaging	Het
Map2k4	T	A	11: 65,666,583 (GRCm39)	I40L	unknown	Het
Map7d1	A	G	4: 126,130,429 (GRCm39)	L459P	probably damaging	Het
Mipol1	A	T	12: 57,352,859 (GRCm39)	N66I	possibly damaging	Het
Mycn	A	C	12: 12,990,292 (GRCm39)	F35V	probably benign	Het
Mzt2	A	C	16: 15,680,669 (GRCm39)	M40R	possibly damaging	Het
N4bp2	T	G	5: 65,948,458 (GRCm39)	L363V	probably damaging	Het
Nfat5	T	A	8: 108,097,221 (GRCm39)	M1532K	probably benign	Het
Notch1	G	A	2: 26,350,177 (GRCm39)	T2321M	probably benign	Het
Nr1h4	T	C	10: 89,334,123 (GRCm39)	D89G	probably benign	Het
Nsun4	G	A	4: 115,908,691 (GRCm39)	T623I	possibly damaging	Het
Nup155	T	C	15: 8,184,531 (GRCm39)	S1309P	probably benign	Het
Or4e1	T	C	14: 52,700,851 (GRCm39)	N205S	probably damaging	Het
Or51f5	T	C	7: 102,430,889 (GRCm39)	S69P	probably damaging	Het
Or8h10	T	C	2: 86,809,122 (GRCm39)	Y6C	probably benign	Het
Pcsk5	A	G	19: 17,814,336 (GRCm39)	F61L	probably benign	Het
Phrf1	A	T	7: 140,811,138 (GRCm39)		probably null	Het
Pkd1	G	A	17: 24,792,605 (GRCm39)	E1431K	probably benign	Het
Pkd2	T	A	5: 104,628,219 (GRCm39)	I431N	probably damaging	Het
Plcb2	A	G	2: 118,546,124 (GRCm39)	L557P	probably damaging	Het
Poteg	A	G	8: 27,984,988 (GRCm39)	N449S	probably benign	Het
Ppargc1b	C	A	18: 61,445,281 (GRCm39)	G192W	probably damaging	Het
Rab13	T	C	3: 90,132,206 (GRCm39)	S178P	probably benign	Het
Ralgds	A	G	2: 28,437,607 (GRCm39)	D571G	probably damaging	Het
Ror2	A	G	13: 53,264,849 (GRCm39)	S748P	probably benign	Het
Sart1	A	T	19: 5,434,905 (GRCm39)	L167Q	probably damaging	Het
Septin12	A	G	16: 4,810,055 (GRCm39)	V160A	possibly damaging	Het
Sgo1	T	C	17: 53,986,295 (GRCm39)	S299G	probably benign	Het
Shoc1	T	C	4: 59,076,140 (GRCm39)	N601S	possibly damaging	Het
Slc35b4	T	A	6: 34,140,296 (GRCm39)	D152V	probably benign	Het
Spata31h1	T	C	10: 82,120,449 (GRCm39)	Y4187C	probably damaging	Het
Specc1	T	A	11: 62,019,235 (GRCm39)		probably null	Het
Synj1	G	A	16: 90,737,371 (GRCm39)	T1308I	probably benign	Het
Thap12	T	G	7: 98,359,438 (GRCm39)	L106V	probably damaging	Het
Togaram1	T	C	12: 65,057,916 (GRCm39)	M1501T	possibly damaging	Het
Top2a	G	T	11: 98,891,663 (GRCm39)	N1153K	probably benign	Het
Trpm5	A	G	7: 142,634,723 (GRCm39)	L658P	probably damaging	Het
Ttc21b	G	A	2: 66,047,548 (GRCm39)	A849V	possibly damaging	Het
Vcf1	C	T	11: 113,554,215 (GRCm39)	G160D	probably damaging	Het
Vmn1r80	A	T	7: 11,927,750 (GRCm39)	I287F	probably damaging	Het
Vmn1r87	T	C	7: 12,865,745 (GRCm39)	S181G	probably damaging	Het
Vmn2r71	A	T	7: 85,273,115 (GRCm39)	H643L	probably benign	Het
Washc5	G	A	15: 59,238,041 (GRCm39)	R228C	probably damaging	Het
Wdr72	T	C	9: 74,117,408 (GRCm39)	S719P	probably damaging	Het
Xrcc3	G	T	12: 111,774,336 (GRCm39)	D164E	probably benign	Het
Zc2hc1b	G	A	10: 13,044,529 (GRCm39)	R45C	probably damaging	Het
Zfp335	A	G	2: 164,737,912 (GRCm39)	Y900H	probably damaging	Het
Zfp512b	G	A	2: 181,228,875 (GRCm39)	R697C	probably damaging	Het
Zfp60	T	C	7: 27,448,561 (GRCm39)	Y410H	probably damaging	Het
Zfp60	T	A	7: 27,448,696 (GRCm39)	C455S	probably damaging	Het
Zfp605	C	T	5: 110,275,157 (GRCm39)	Q92*	probably null	Het
Zfp64	C	T	2: 168,767,585 (GRCm39)	D676N	probably damaging	Het

Other mutations in Hectd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Hectd4	APN	5	121,501,933 (GRCm39)	missense	possibly damaging	0.51
IGL00976:Hectd4	APN	5	121,487,169 (GRCm39)	missense	probably benign	0.18
IGL01085:Hectd4	APN	5	121,469,764 (GRCm39)	missense	probably damaging	1.00
IGL01112:Hectd4	APN	5	121,445,013 (GRCm39)	missense	probably benign	0.01
IGL01402:Hectd4	APN	5	121,477,480 (GRCm39)	splice site	probably benign
IGL01474:Hectd4	APN	5	121,474,712 (GRCm39)	missense	possibly damaging	0.53
IGL01503:Hectd4	APN	5	121,456,714 (GRCm39)	missense	probably benign	0.28
IGL01548:Hectd4	APN	5	121,502,723 (GRCm39)	missense	possibly damaging	0.71
IGL01656:Hectd4	APN	5	121,460,763 (GRCm39)	missense	probably damaging	0.99
IGL01756:Hectd4	APN	5	121,482,887 (GRCm39)	missense	probably benign	0.28
IGL01819:Hectd4	APN	5	121,466,481 (GRCm39)	missense	possibly damaging	0.85
IGL02080:Hectd4	APN	5	121,504,669 (GRCm39)	utr 3 prime	probably benign
IGL02488:Hectd4	APN	5	121,430,150 (GRCm39)	missense	probably benign	0.33
IGL02490:Hectd4	APN	5	121,456,676 (GRCm39)	missense	possibly damaging	0.82
IGL02558:Hectd4	APN	5	121,482,848 (GRCm39)	missense	probably benign	0.28
IGL02626:Hectd4	APN	5	121,491,944 (GRCm39)	missense	possibly damaging	0.86
IGL02649:Hectd4	APN	5	121,487,465 (GRCm39)	missense	possibly damaging	0.73
IGL02736:Hectd4	APN	5	121,480,782 (GRCm39)	missense	possibly damaging	0.73
IGL02861:Hectd4	APN	5	121,445,067 (GRCm39)	missense	possibly damaging	0.81
IGL02880:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02889:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02953:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02969:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03031:Hectd4	APN	5	121,486,857 (GRCm39)	missense	possibly damaging	0.96
IGL03066:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03160:Hectd4	APN	5	121,397,942 (GRCm39)	missense	probably benign
IGL03181:Hectd4	APN	5	121,492,021 (GRCm39)	missense	possibly damaging	0.91
IGL03265:Hectd4	APN	5	121,398,002 (GRCm39)	splice site	probably benign
IGL03375:Hectd4	APN	5	121,466,445 (GRCm39)	missense	possibly damaging	0.72
Achilles	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
agamemnon	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
clymnestra	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
hector	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
helen	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
Merriwether	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
PIT4466001:Hectd4	UTSW	5	121,471,123 (GRCm39)	critical splice donor site	probably null
R0018:Hectd4	UTSW	5	121,392,242 (GRCm39)	missense	possibly damaging	0.53
R0024:Hectd4	UTSW	5	121,446,639 (GRCm39)	missense	possibly damaging	0.92
R0030:Hectd4	UTSW	5	121,400,651 (GRCm39)	nonsense	probably null
R0080:Hectd4	UTSW	5	121,487,435 (GRCm39)	missense	probably benign	0.18
R0110:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0110:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0115:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0128:Hectd4	UTSW	5	121,487,306 (GRCm39)	missense	possibly damaging	0.86
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0132:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0244:Hectd4	UTSW	5	121,467,668 (GRCm39)	missense	probably benign	0.33
R0281:Hectd4	UTSW	5	121,392,314 (GRCm39)	missense	possibly damaging	0.85
R0329:Hectd4	UTSW	5	121,397,927 (GRCm39)	missense	probably benign
R0410:Hectd4	UTSW	5	121,424,329 (GRCm39)	missense	possibly damaging	0.86
R0422:Hectd4	UTSW	5	121,481,145 (GRCm39)	splice site	probably null
R0442:Hectd4	UTSW	5	121,462,045 (GRCm39)	missense	possibly damaging	0.66
R0449:Hectd4	UTSW	5	121,502,653 (GRCm39)	splice site	probably null
R0469:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0469:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0481:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0510:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0510:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0520:Hectd4	UTSW	5	121,469,770 (GRCm39)	missense	possibly damaging	0.53
R0534:Hectd4	UTSW	5	121,486,539 (GRCm39)	missense	possibly damaging	0.96
R0603:Hectd4	UTSW	5	121,442,400 (GRCm39)	missense	possibly damaging	0.46
R0617:Hectd4	UTSW	5	121,481,295 (GRCm39)	splice site	probably benign
R0622:Hectd4	UTSW	5	121,486,688 (GRCm39)	missense	possibly damaging	0.53
R0626:Hectd4	UTSW	5	121,415,887 (GRCm39)	missense	probably benign	0.18
R0708:Hectd4	UTSW	5	121,424,526 (GRCm39)	critical splice donor site	probably null
R0710:Hectd4	UTSW	5	121,474,691 (GRCm39)	missense	probably benign	0.08
R0763:Hectd4	UTSW	5	121,445,096 (GRCm39)	unclassified	probably benign
R0764:Hectd4	UTSW	5	121,424,832 (GRCm39)	missense	possibly damaging	0.46
R1123:Hectd4	UTSW	5	121,424,799 (GRCm39)	missense	probably damaging	0.96
R1129:Hectd4	UTSW	5	121,448,662 (GRCm39)	missense	possibly damaging	0.66
R1204:Hectd4	UTSW	5	121,488,548 (GRCm39)	missense	possibly damaging	0.85
R1237:Hectd4	UTSW	5	121,459,570 (GRCm39)	missense	possibly damaging	0.90
R1257:Hectd4	UTSW	5	121,456,687 (GRCm39)	nonsense	probably null
R1391:Hectd4	UTSW	5	121,491,758 (GRCm39)	missense	possibly damaging	0.96
R1395:Hectd4	UTSW	5	121,466,576 (GRCm39)	critical splice donor site	probably null
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1545:Hectd4	UTSW	5	121,462,019 (GRCm39)	missense	possibly damaging	0.87
R1553:Hectd4	UTSW	5	121,487,322 (GRCm39)	missense	probably benign	0.00
R1572:Hectd4	UTSW	5	121,439,941 (GRCm39)	missense	possibly damaging	0.85
R1662:Hectd4	UTSW	5	121,455,308 (GRCm39)	missense	probably benign	0.01
R1705:Hectd4	UTSW	5	121,436,167 (GRCm39)	missense	probably benign
R1715:Hectd4	UTSW	5	121,482,881 (GRCm39)	missense	possibly damaging	0.85
R1728:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1736:Hectd4	UTSW	5	121,487,593 (GRCm39)	missense	possibly damaging	0.53
R1768:Hectd4	UTSW	5	121,496,366 (GRCm39)	missense	possibly damaging	0.70
R1775:Hectd4	UTSW	5	121,429,254 (GRCm39)	splice site	probably benign
R1784:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1843:Hectd4	UTSW	5	121,435,243 (GRCm39)	missense	possibly damaging	0.53
R1914:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R1915:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R2024:Hectd4	UTSW	5	121,419,981 (GRCm39)	missense	possibly damaging	0.86
R2103:Hectd4	UTSW	5	121,493,692 (GRCm39)	missense	probably benign	0.04
R2108:Hectd4	UTSW	5	121,471,487 (GRCm39)	missense	possibly damaging	0.72
R2124:Hectd4	UTSW	5	121,456,702 (GRCm39)	missense	probably damaging	0.97
R2150:Hectd4	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
R2192:Hectd4	UTSW	5	121,453,206 (GRCm39)	missense	possibly damaging	0.46
R2301:Hectd4	UTSW	5	121,491,600 (GRCm39)	missense	probably benign	0.18
R2324:Hectd4	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
R2331:Hectd4	UTSW	5	121,458,089 (GRCm39)	missense	probably benign	0.05
R2504:Hectd4	UTSW	5	121,402,030 (GRCm39)	missense	possibly damaging	0.73
R2504:Hectd4	UTSW	5	121,358,683 (GRCm39)	missense	unknown
R2904:Hectd4	UTSW	5	121,430,787 (GRCm39)	splice site	probably benign
R3843:Hectd4	UTSW	5	121,397,936 (GRCm39)	missense	possibly damaging	0.72
R3934:Hectd4	UTSW	5	121,458,164 (GRCm39)	critical splice donor site	probably null
R3944:Hectd4	UTSW	5	121,441,588 (GRCm39)	splice site	probably benign
R4133:Hectd4	UTSW	5	121,415,897 (GRCm39)	critical splice donor site	probably null
R4271:Hectd4	UTSW	5	121,358,567 (GRCm39)	small deletion	probably benign
R4413:Hectd4	UTSW	5	121,488,544 (GRCm39)	missense	possibly damaging	0.53
R4456:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R4489:Hectd4	UTSW	5	121,424,320 (GRCm39)	missense	possibly damaging	0.73
R4539:Hectd4	UTSW	5	121,452,970 (GRCm39)	nonsense	probably null
R4564:Hectd4	UTSW	5	121,488,494 (GRCm39)	missense	probably benign	0.33
R4582:Hectd4	UTSW	5	121,424,482 (GRCm39)	missense	possibly damaging	0.53
R4629:Hectd4	UTSW	5	121,435,266 (GRCm39)	missense	probably benign	0.01
R4633:Hectd4	UTSW	5	121,487,279 (GRCm39)	missense	probably benign	0.33
R4643:Hectd4	UTSW	5	121,487,118 (GRCm39)	missense	possibly damaging	0.53
R4679:Hectd4	UTSW	5	121,463,314 (GRCm39)	missense	possibly damaging	0.72
R4681:Hectd4	UTSW	5	121,441,678 (GRCm39)	missense	possibly damaging	0.86
R4734:Hectd4	UTSW	5	121,480,040 (GRCm39)	missense	possibly damaging	0.53
R4739:Hectd4	UTSW	5	121,486,505 (GRCm39)	missense	probably benign
R4781:Hectd4	UTSW	5	121,444,170 (GRCm39)	critical splice donor site	probably null
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4869:Hectd4	UTSW	5	121,460,735 (GRCm39)	missense	possibly damaging	0.46
R4909:Hectd4	UTSW	5	121,401,954 (GRCm39)	missense	probably benign	0.18
R4922:Hectd4	UTSW	5	121,497,378 (GRCm39)	missense	possibly damaging	0.86
R4925:Hectd4	UTSW	5	121,460,753 (GRCm39)	missense	possibly damaging	0.83
R5004:Hectd4	UTSW	5	121,467,628 (GRCm39)	missense	possibly damaging	0.93
R5004:Hectd4	UTSW	5	121,466,262 (GRCm39)	splice site	probably null
R5129:Hectd4	UTSW	5	121,481,573 (GRCm39)	missense	possibly damaging	0.87
R5217:Hectd4	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
R5267:Hectd4	UTSW	5	121,482,887 (GRCm39)	missense	probably benign	0.28
R5344:Hectd4	UTSW	5	121,481,739 (GRCm39)	missense	probably benign	0.28
R5345:Hectd4	UTSW	5	121,402,037 (GRCm39)	missense	possibly damaging	0.85
R5347:Hectd4	UTSW	5	121,442,511 (GRCm39)	missense	probably benign	0.33
R5360:Hectd4	UTSW	5	121,453,464 (GRCm39)	missense	possibly damaging	0.90
R5363:Hectd4	UTSW	5	121,448,666 (GRCm39)	missense	probably benign	0.04
R5445:Hectd4	UTSW	5	121,404,337 (GRCm39)	missense	probably benign	0.00
R5479:Hectd4	UTSW	5	121,445,011 (GRCm39)	missense	probably benign
R5507:Hectd4	UTSW	5	121,419,164 (GRCm39)	missense	unknown
R5552:Hectd4	UTSW	5	121,480,914 (GRCm39)	missense	possibly damaging	0.96
R5691:Hectd4	UTSW	5	121,486,878 (GRCm39)	missense	possibly damaging	0.85
R5745:Hectd4	UTSW	5	121,491,565 (GRCm39)	missense	possibly damaging	0.96
R5757:Hectd4	UTSW	5	121,486,682 (GRCm39)	missense	possibly damaging	0.72
R5845:Hectd4	UTSW	5	121,445,587 (GRCm39)	critical splice donor site	probably null
R5869:Hectd4	UTSW	5	121,481,288 (GRCm39)	critical splice donor site	probably null
R5913:Hectd4	UTSW	5	121,462,037 (GRCm39)	missense	possibly damaging	0.83
R5920:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R5943:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.01
R6219:Hectd4	UTSW	5	121,446,941 (GRCm39)	missense	possibly damaging	0.92
R6250:Hectd4	UTSW	5	121,477,561 (GRCm39)	missense	possibly damaging	0.85
R6301:Hectd4	UTSW	5	121,392,283 (GRCm39)	missense	possibly damaging	0.91
R6428:Hectd4	UTSW	5	121,488,508 (GRCm39)	missense	possibly damaging	0.53
R6446:Hectd4	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
R6453:Hectd4	UTSW	5	121,488,655 (GRCm39)	missense	probably damaging	1.00
R6513:Hectd4	UTSW	5	121,494,259 (GRCm39)	splice site	probably null
R6540:Hectd4	UTSW	5	121,441,634 (GRCm39)	missense	probably benign	0.33
R6706:Hectd4	UTSW	5	121,458,147 (GRCm39)	missense	possibly damaging	0.92
R6720:Hectd4	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
R6736:Hectd4	UTSW	5	121,415,788 (GRCm39)	missense	possibly damaging	0.86
R6776:Hectd4	UTSW	5	121,491,574 (GRCm39)	missense	possibly damaging	0.85
R7033:Hectd4	UTSW	5	121,502,631 (GRCm39)	missense	possibly damaging	0.86
R7038:Hectd4	UTSW	5	121,437,660 (GRCm39)	missense	possibly damaging	0.90
R7175:Hectd4	UTSW	5	121,411,692 (GRCm39)	missense	possibly damaging	0.85
R7180:Hectd4	UTSW	5	121,446,405 (GRCm39)	missense	probably benign	0.01
R7234:Hectd4	UTSW	5	121,467,136 (GRCm39)	missense	possibly damaging	0.53
R7253:Hectd4	UTSW	5	121,452,944 (GRCm39)	missense	possibly damaging	0.66
R7349:Hectd4	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
R7450:Hectd4	UTSW	5	121,419,995 (GRCm39)	missense	probably benign	0.00
R7467:Hectd4	UTSW	5	121,462,024 (GRCm39)	missense	possibly damaging	0.66
R7475:Hectd4	UTSW	5	121,496,196 (GRCm39)	splice site	probably null
R7482:Hectd4	UTSW	5	121,501,941 (GRCm39)	missense	possibly damaging	0.71
R7512:Hectd4	UTSW	5	121,435,172 (GRCm39)	missense	possibly damaging	0.72
R7525:Hectd4	UTSW	5	121,481,728 (GRCm39)	missense	possibly damaging	0.70
R7559:Hectd4	UTSW	5	121,453,573 (GRCm39)	splice site	probably null
R7561:Hectd4	UTSW	5	121,429,288 (GRCm39)	missense	possibly damaging	0.91
R7576:Hectd4	UTSW	5	121,487,522 (GRCm39)	missense	possibly damaging	0.91
R7584:Hectd4	UTSW	5	121,456,798 (GRCm39)	missense	possibly damaging	0.83
R7648:Hectd4	UTSW	5	121,392,434 (GRCm39)	missense	possibly damaging	0.73
R7663:Hectd4	UTSW	5	121,462,094 (GRCm39)	missense	probably benign	0.06
R7692:Hectd4	UTSW	5	121,459,627 (GRCm39)	missense	possibly damaging	0.46
R7725:Hectd4	UTSW	5	121,358,680 (GRCm39)	missense	unknown
R7731:Hectd4	UTSW	5	121,445,077 (GRCm39)	missense	probably benign	0.00
R7732:Hectd4	UTSW	5	121,474,692 (GRCm39)	missense	probably benign	0.14
R7782:Hectd4	UTSW	5	121,443,784 (GRCm39)	missense	possibly damaging	0.53
R7854:Hectd4	UTSW	5	121,467,631 (GRCm39)	missense	probably benign	0.27
R7898:Hectd4	UTSW	5	121,469,880 (GRCm39)	missense	probably benign	0.18
R7910:Hectd4	UTSW	5	121,392,291 (GRCm39)	missense	possibly damaging	0.86
R7962:Hectd4	UTSW	5	121,448,692 (GRCm39)	missense	probably damaging	0.98
R8003:Hectd4	UTSW	5	121,477,581 (GRCm39)	missense	possibly damaging	0.85
R8098:Hectd4	UTSW	5	121,459,461 (GRCm39)	missense	possibly damaging	0.46
R8110:Hectd4	UTSW	5	121,471,012 (GRCm39)	missense	possibly damaging	0.96
R8118:Hectd4	UTSW	5	121,424,439 (GRCm39)	missense	probably benign	0.33
R8171:Hectd4	UTSW	5	121,456,819 (GRCm39)	missense	possibly damaging	0.82
R8234:Hectd4	UTSW	5	121,477,607 (GRCm39)	missense	possibly damaging	0.72
R8289:Hectd4	UTSW	5	121,404,424 (GRCm39)	missense	possibly damaging	0.53
R8292:Hectd4	UTSW	5	121,455,288 (GRCm39)	missense	possibly damaging	0.66
R8348:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8397:Hectd4	UTSW	5	121,397,957 (GRCm39)	missense	probably damaging	0.98
R8436:Hectd4	UTSW	5	121,446,421 (GRCm39)	missense	possibly damaging	0.90
R8436:Hectd4	UTSW	5	121,481,210 (GRCm39)	missense	probably benign	0.00
R8443:Hectd4	UTSW	5	121,467,172 (GRCm39)	missense	possibly damaging	0.72
R8448:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8516:Hectd4	UTSW	5	121,487,073 (GRCm39)	missense	possibly damaging	0.53
R8519:Hectd4	UTSW	5	121,442,489 (GRCm39)	nonsense	probably null
R8553:Hectd4	UTSW	5	121,491,661 (GRCm39)	missense	possibly damaging	0.73
R8557:Hectd4	UTSW	5	121,448,714 (GRCm39)	missense	possibly damaging	0.66
R8725:Hectd4	UTSW	5	121,488,557 (GRCm39)	missense	probably damaging	1.00
R8751:Hectd4	UTSW	5	121,501,838 (GRCm39)	nonsense	probably null
R8769:Hectd4	UTSW	5	121,419,936 (GRCm39)	missense	possibly damaging	0.53
R8803:Hectd4	UTSW	5	121,461,994 (GRCm39)	missense	probably benign	0.01
R8887:Hectd4	UTSW	5	121,433,541 (GRCm39)	missense	probably benign	0.44
R8982:Hectd4	UTSW	5	121,466,305 (GRCm39)	missense	probably benign	0.02
R8988:Hectd4	UTSW	5	121,415,819 (GRCm39)	missense	possibly damaging	0.86
R8991:Hectd4	UTSW	5	121,496,347 (GRCm39)	missense	probably benign	0.33
R8994:Hectd4	UTSW	5	121,441,629 (GRCm39)	missense	probably benign	0.33
R8995:Hectd4	UTSW	5	121,392,422 (GRCm39)	missense	possibly damaging	0.96
R9049:Hectd4	UTSW	5	121,451,955 (GRCm39)	missense	possibly damaging	0.92
R9093:Hectd4	UTSW	5	121,411,677 (GRCm39)	missense	probably benign	0.14
R9106:Hectd4	UTSW	5	121,467,619 (GRCm39)	missense	possibly damaging	0.53
R9137:Hectd4	UTSW	5	121,496,238 (GRCm39)	missense	possibly damaging	0.53
R9146:Hectd4	UTSW	5	121,487,097 (GRCm39)	missense	probably benign	0.33
R9154:Hectd4	UTSW	5	121,391,967 (GRCm39)	missense
R9162:Hectd4	UTSW	5	121,445,042 (GRCm39)	missense	possibly damaging	0.66
R9166:Hectd4	UTSW	5	121,446,690 (GRCm39)	missense	probably damaging	0.96
R9183:Hectd4	UTSW	5	121,437,551 (GRCm39)	missense	possibly damaging	0.51
R9207:Hectd4	UTSW	5	121,433,496 (GRCm39)	missense	possibly damaging	0.86
R9291:Hectd4	UTSW	5	121,487,028 (GRCm39)	missense	probably benign	0.14
R9300:Hectd4	UTSW	5	121,486,952 (GRCm39)	missense	probably benign	0.33
R9314:Hectd4	UTSW	5	121,437,708 (GRCm39)	critical splice donor site	probably null
R9381:Hectd4	UTSW	5	121,472,492 (GRCm39)	missense	possibly damaging	0.53
R9432:Hectd4	UTSW	5	121,460,864 (GRCm39)	missense	probably benign	0.01
R9491:Hectd4	UTSW	5	121,452,981 (GRCm39)	missense	probably damaging	0.97
R9532:Hectd4	UTSW	5	121,502,616 (GRCm39)	missense	probably benign	0.00
R9557:Hectd4	UTSW	5	121,459,617 (GRCm39)	missense	possibly damaging	0.66
R9561:Hectd4	UTSW	5	121,472,532 (GRCm39)	missense	possibly damaging	0.53
R9593:Hectd4	UTSW	5	121,424,844 (GRCm39)	nonsense	probably null
R9704:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9705:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9712:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9713:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9726:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9732:Hectd4	UTSW	5	121,392,254 (GRCm39)	nonsense	probably null
R9750:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9752:Hectd4	UTSW	5	121,472,415 (GRCm39)	missense	possibly damaging	0.85
R9752:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9772:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
X0026:Hectd4	UTSW	5	121,487,700 (GRCm39)	missense	probably benign	0.04
X0027:Hectd4	UTSW	5	121,459,467 (GRCm39)	missense	probably benign	0.27
Z1088:Hectd4	UTSW	5	121,433,566 (GRCm39)	splice site	probably null
Z1177:Hectd4	UTSW	5	121,496,383 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TCCCTCTGTAACATAACTGCTG -3'
(R):5'- ATCAGCTACAGGCAAGGCTAC -3'

Sequencing Primer
(F):5'- CCTCTGTAACATAACTGCTGAGGTG -3'
(R):5'- AAGGCTACTACTCCAGGCTGTC -3'

Posted On

2019-10-17