Mutagenetix > Incidental Mutation

Incidental Mutation 'R0617:Hectd4'

58416

Institutional Source

Beutler Lab

Gene Symbol

Hectd4

Ensembl Gene

ENSMUSG00000042744

Gene Name

HECT domain E3 ubiquitin protein ligase 4

Synonyms

Gm15800

MMRRC Submission

038806-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.927) question?

Stock #

R0617 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

121358282-121506640 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 121481295 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000048345 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042614]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000042614

SMART Domains

Protein: ENSMUSP00000048345
Gene: ENSMUSG00000042744

Domain	Start	End	E-Value	Type
low complexity region	224	234	N/A	INTRINSIC
low complexity region	266	282	N/A	INTRINSIC
low complexity region	553	564	N/A	INTRINSIC
low complexity region	725	735	N/A	INTRINSIC
low complexity region	1252	1265	N/A	INTRINSIC
coiled coil region	1372	1398	N/A	INTRINSIC
low complexity region	1551	1562	N/A	INTRINSIC
low complexity region	1725	1741	N/A	INTRINSIC
low complexity region	1892	1904	N/A	INTRINSIC
low complexity region	2656	2666	N/A	INTRINSIC
low complexity region	2857	2872	N/A	INTRINSIC
low complexity region	2901	2917	N/A	INTRINSIC
low complexity region	2921	2933	N/A	INTRINSIC
low complexity region	3232	3246	N/A	INTRINSIC
low complexity region	3275	3335	N/A	INTRINSIC
low complexity region	3441	3448	N/A	INTRINSIC
low complexity region	3473	3506	N/A	INTRINSIC
low complexity region	3512	3533	N/A	INTRINSIC
low complexity region	3540	3554	N/A	INTRINSIC
low complexity region	3794	3822	N/A	INTRINSIC
HECTc	4048	4412	4.78e-11	SMART

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.5%

Validation Efficiency

98% (130/133)

Allele List at MGI

Other mutations in this stock

Total: 130 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930555F03Rik	A	T	8: 49,953,527 (GRCm39)		noncoding transcript	Het
A630073D07Rik	T	C	6: 132,603,700 (GRCm39)		probably benign	Het
Abca16	G	A	7: 120,032,834 (GRCm39)		probably benign	Het
Abca5	A	T	11: 110,170,515 (GRCm39)	D1265E	probably damaging	Het
Abcf1	C	T	17: 36,272,079 (GRCm39)	V312I	probably benign	Het
Abhd12	T	A	2: 150,688,285 (GRCm39)		probably null	Het
Adam23	A	G	1: 63,582,306 (GRCm39)	H318R	probably benign	Het
Adcy2	T	A	13: 68,826,725 (GRCm39)	K660*	probably null	Het
Adgrf3	T	C	5: 30,400,078 (GRCm39)	T972A	probably benign	Het
Adipoq	T	A	16: 22,974,160 (GRCm39)	D62E	probably damaging	Het
Alk	G	T	17: 72,910,578 (GRCm39)	P43T	probably damaging	Het
Arap2	AT	ATT	5: 62,807,250 (GRCm39)		probably benign	Het
Arhgef28	G	T	13: 98,106,863 (GRCm39)	T687K	probably benign	Het
Arrb1	T	C	7: 99,243,884 (GRCm39)	L278P	probably damaging	Het
Atad2b	C	A	12: 4,987,401 (GRCm39)	D76E	probably benign	Het
Atm	A	T	9: 53,370,241 (GRCm39)	Y2290*	probably null	Het
Atrn	T	A	2: 130,837,005 (GRCm39)		probably null	Het
Bpifb1	A	G	2: 154,054,867 (GRCm39)	D253G	possibly damaging	Het
Bpifb9b	C	T	2: 154,161,545 (GRCm39)	T559M	probably benign	Het
Bsn	T	C	9: 107,984,439 (GRCm39)	E3205G	unknown	Het
Cacna1c	T	C	6: 118,579,174 (GRCm39)	Y1599C	probably damaging	Het
Ccdc40	A	G	11: 119,133,630 (GRCm39)	D590G	probably damaging	Het
Ccdc68	A	G	18: 70,079,623 (GRCm39)		probably null	Het
Ccdc97	G	A	7: 25,413,845 (GRCm39)	R279C	probably damaging	Het
Ccm2l	A	G	2: 152,912,820 (GRCm39)	T120A	probably damaging	Het
Cfap54	T	C	10: 92,665,512 (GRCm39)		probably benign	Het
Cfh	A	G	1: 140,028,621 (GRCm39)	S1043P	probably benign	Het
Chil3	T	C	3: 106,063,072 (GRCm39)	K173E	probably benign	Het
Cib2	T	C	9: 54,461,780 (GRCm39)	D26G	possibly damaging	Het
Col24a1	T	C	3: 145,019,881 (GRCm39)	V84A	probably damaging	Het
Csn3	T	C	5: 88,077,730 (GRCm39)	Y79H	probably benign	Het
Ddx47	T	A	6: 134,994,085 (GRCm39)	V149E	probably damaging	Het
Dennd5b	A	T	6: 148,934,760 (GRCm39)		probably benign	Het
Desi1	T	C	15: 81,882,399 (GRCm39)	N109D	probably damaging	Het
Fam13c	T	C	10: 70,372,182 (GRCm39)		probably benign	Het
Fam234a	A	T	17: 26,435,591 (GRCm39)	D264E	probably benign	Het
Fanca	A	G	8: 124,014,809 (GRCm39)	F831S	probably damaging	Het
Fancm	C	T	12: 65,144,091 (GRCm39)	R518*	probably null	Het
Fat2	A	G	11: 55,202,669 (GRCm39)	V135A	possibly damaging	Het
Fbxl17	A	C	17: 63,691,987 (GRCm39)	F42V	probably damaging	Het
Fgd3	A	T	13: 49,418,173 (GRCm39)	V631E	possibly damaging	Het
Fhod3	G	A	18: 25,245,736 (GRCm39)		probably benign	Het
Focad	T	C	4: 88,039,525 (GRCm39)		probably benign	Het
Foxn4	C	A	5: 114,399,129 (GRCm39)		probably benign	Het
Gm2381	A	T	7: 42,469,402 (GRCm39)	C241S	probably damaging	Het
Gm6483	T	G	8: 19,743,725 (GRCm39)	F117V	probably damaging	Het
Hecw1	T	A	13: 14,455,027 (GRCm39)	Q676L	probably benign	Het
Hipk2	G	A	6: 38,724,420 (GRCm39)	R437C	possibly damaging	Het
Ifnar1	T	C	16: 91,298,570 (GRCm39)	Y396H	probably damaging	Het
Ints5	A	T	19: 8,873,383 (GRCm39)	K447N	probably damaging	Het
Iqsec1	T	C	6: 90,666,952 (GRCm39)	Y495C	probably damaging	Het
Itga5	C	T	15: 103,264,742 (GRCm39)		probably null	Het
Kcnk4	T	A	19: 6,905,528 (GRCm39)		probably benign	Het
Kmo	A	G	1: 175,474,756 (GRCm39)	T174A	possibly damaging	Het
Krt36	T	C	11: 99,993,101 (GRCm39)	D458G	probably damaging	Het
Krtap16-1	G	T	11: 99,877,321 (GRCm39)	P28T	probably damaging	Het
Lama3	T	C	18: 12,552,315 (GRCm39)		probably null	Het
Lrrc9	T	C	12: 72,529,788 (GRCm39)	S920P	probably damaging	Het
Lrrk2	A	T	15: 91,636,481 (GRCm39)	Y1485F	probably benign	Het
Mical1	C	T	10: 41,357,311 (GRCm39)	A372V	probably damaging	Het
Ms4a20	C	A	19: 11,089,764 (GRCm39)	L40F	probably damaging	Het
Mtr	C	T	13: 12,236,318 (GRCm39)	R636Q	probably benign	Het
Muc4	A	T	16: 32,570,925 (GRCm39)	T662S	possibly damaging	Het
Myo10	G	A	15: 25,738,091 (GRCm39)	V546M	probably damaging	Het
Nbeal1	G	A	1: 60,320,991 (GRCm39)	W2034*	probably null	Het
Nhlrc3	T	C	3: 53,366,044 (GRCm39)	T150A	probably damaging	Het
Nicol1	T	C	5: 34,140,896 (GRCm39)		probably benign	Het
Nkx2-1	T	C	12: 56,581,640 (GRCm39)	H69R	possibly damaging	Het
Nlrp4g	A	T	9: 124,349,540 (GRCm38)		noncoding transcript	Het
Nod2	A	G	8: 89,379,859 (GRCm39)	N120S	probably benign	Het
Nol8	T	C	13: 49,807,921 (GRCm39)	F46L	possibly damaging	Het
Ntrk1	T	C	3: 87,691,240 (GRCm39)	D308G	possibly damaging	Het
Oog3	A	T	4: 143,886,784 (GRCm39)	V112D	probably benign	Het
Or10ag58	A	G	2: 87,265,005 (GRCm39)	D58G	probably damaging	Het
Or4a66	A	G	2: 88,531,040 (GRCm39)	V211A	probably damaging	Het
Or51i1	A	T	7: 103,671,196 (GRCm39)	S110T	probably damaging	Het
Or5af2	T	C	11: 58,707,975 (GRCm39)	V47A	probably damaging	Het
Or5b106	T	C	19: 13,123,727 (GRCm39)	M99V	probably benign	Het
Or5b120	C	A	19: 13,479,900 (GRCm39)	N64K	probably damaging	Het
Or5m9b	A	G	2: 85,905,485 (GRCm39)	M134V	probably benign	Het
Or9s18	A	T	13: 65,300,692 (GRCm39)	Y218F	possibly damaging	Het
Pakap	T	C	4: 57,829,434 (GRCm39)		probably benign	Het
Pcdhb8	C	T	18: 37,490,100 (GRCm39)	R593C	probably benign	Het
Pgm3	A	G	9: 86,438,243 (GRCm39)		probably null	Het
Pirt	T	A	11: 66,816,998 (GRCm39)	V103E	probably damaging	Het
Plxnc1	T	A	10: 94,635,230 (GRCm39)	D1332V	probably damaging	Het
Ppfia4	A	T	1: 134,256,518 (GRCm39)	V122E	probably damaging	Het
Pramel14	A	G	4: 143,720,088 (GRCm39)		probably benign	Het
Prmt2	C	T	10: 76,044,517 (GRCm39)		probably benign	Het
Prrc2a	G	T	17: 35,372,536 (GRCm39)	P1702T	probably damaging	Het
Prss39	A	T	1: 34,539,279 (GRCm39)	H173L	probably damaging	Het
Rabl6	A	G	2: 25,476,878 (GRCm39)		probably null	Het
Rb1cc1	T	A	1: 6,319,014 (GRCm39)	I794K	possibly damaging	Het
Reln	T	C	5: 22,125,535 (GRCm39)	D2716G	probably damaging	Het
Sbf2	ACC	AC	7: 109,929,890 (GRCm39)		probably null	Het
Sema6d	T	A	2: 124,502,665 (GRCm39)	F583L	possibly damaging	Het
Setx	T	A	2: 29,036,819 (GRCm39)	H1101Q	possibly damaging	Het
Sis	A	G	3: 72,872,938 (GRCm39)	C67R	probably damaging	Het
Skint1	T	A	4: 111,886,596 (GRCm39)		probably benign	Het
Smg6	C	A	11: 75,053,757 (GRCm39)	T1413K	probably benign	Het
Spata31d1a	A	T	13: 59,850,073 (GRCm39)	I685N	possibly damaging	Het
Spef2	T	A	15: 9,592,844 (GRCm39)	N1499I	probably damaging	Het
Stk11ip	T	A	1: 75,508,932 (GRCm39)		probably null	Het
Stxbp1	A	C	2: 32,692,795 (GRCm39)	I407S	probably damaging	Het
Svil	T	C	18: 5,117,002 (GRCm39)	S2059P	probably damaging	Het
Syne1	C	T	10: 5,300,933 (GRCm39)	V932M	probably damaging	Het
Tacc1	A	C	8: 25,668,020 (GRCm39)		probably benign	Het
Tbc1d13	C	A	2: 30,025,576 (GRCm39)		probably benign	Het
Tbc1d15	A	C	10: 115,075,204 (GRCm39)	D59E	probably damaging	Het
Tcaf2	A	G	6: 42,619,445 (GRCm39)	F194S	probably damaging	Het
Terf2ip	T	A	8: 112,738,127 (GRCm39)	M5K	probably benign	Het
Tgfbr2	A	T	9: 115,987,388 (GRCm39)	D40E	probably benign	Het
Tm4sf5	T	A	11: 70,401,495 (GRCm39)	S165T	probably damaging	Het
Tmprss3	A	T	17: 31,412,886 (GRCm39)	C129S	probably damaging	Het
Tmx2	T	C	2: 84,502,740 (GRCm39)	D256G	probably benign	Het
Tnr	A	G	1: 159,695,673 (GRCm39)	D532G	probably damaging	Het
Tnrc18	T	A	5: 142,762,494 (GRCm39)	H465L	unknown	Het
Togaram2	A	T	17: 72,007,504 (GRCm39)	Q350L	possibly damaging	Het
Topaz1	G	A	9: 122,578,971 (GRCm39)	C627Y	possibly damaging	Het
Tpx2	A	T	2: 152,715,058 (GRCm39)	Q93L	probably benign	Het
Trim54	T	C	5: 31,293,526 (GRCm39)		probably null	Het
Troap	T	A	15: 98,980,541 (GRCm39)	C574S	probably damaging	Het
Tut7	A	T	13: 59,964,669 (GRCm39)		probably null	Het
Ubr4	T	C	4: 139,206,373 (GRCm39)		probably null	Het
Vmn2r51	A	G	7: 9,834,396 (GRCm39)	V214A	possibly damaging	Het
Vmn2r66	A	T	7: 84,644,484 (GRCm39)	M642K	probably benign	Het
Vwa5a	T	A	9: 38,635,191 (GRCm39)	I232N	probably damaging	Het
Zfp820	A	T	17: 22,038,685 (GRCm39)	S214R	probably damaging	Het
Zfp955b	A	T	17: 33,524,437 (GRCm39)	S43R	probably damaging	Het
Zgrf1	C	T	3: 127,381,687 (GRCm39)	T1162M	probably benign	Het

Other mutations in Hectd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Hectd4	APN	5	121,501,933 (GRCm39)	missense	possibly damaging	0.51
IGL00976:Hectd4	APN	5	121,487,169 (GRCm39)	missense	probably benign	0.18
IGL01085:Hectd4	APN	5	121,469,764 (GRCm39)	missense	probably damaging	1.00
IGL01112:Hectd4	APN	5	121,445,013 (GRCm39)	missense	probably benign	0.01
IGL01402:Hectd4	APN	5	121,477,480 (GRCm39)	splice site	probably benign
IGL01474:Hectd4	APN	5	121,474,712 (GRCm39)	missense	possibly damaging	0.53
IGL01503:Hectd4	APN	5	121,456,714 (GRCm39)	missense	probably benign	0.28
IGL01548:Hectd4	APN	5	121,502,723 (GRCm39)	missense	possibly damaging	0.71
IGL01656:Hectd4	APN	5	121,460,763 (GRCm39)	missense	probably damaging	0.99
IGL01756:Hectd4	APN	5	121,482,887 (GRCm39)	missense	probably benign	0.28
IGL01819:Hectd4	APN	5	121,466,481 (GRCm39)	missense	possibly damaging	0.85
IGL02080:Hectd4	APN	5	121,504,669 (GRCm39)	utr 3 prime	probably benign
IGL02488:Hectd4	APN	5	121,430,150 (GRCm39)	missense	probably benign	0.33
IGL02490:Hectd4	APN	5	121,456,676 (GRCm39)	missense	possibly damaging	0.82
IGL02558:Hectd4	APN	5	121,482,848 (GRCm39)	missense	probably benign	0.28
IGL02626:Hectd4	APN	5	121,491,944 (GRCm39)	missense	possibly damaging	0.86
IGL02649:Hectd4	APN	5	121,487,465 (GRCm39)	missense	possibly damaging	0.73
IGL02736:Hectd4	APN	5	121,480,782 (GRCm39)	missense	possibly damaging	0.73
IGL02861:Hectd4	APN	5	121,445,067 (GRCm39)	missense	possibly damaging	0.81
IGL02880:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02889:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02953:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02969:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03031:Hectd4	APN	5	121,486,857 (GRCm39)	missense	possibly damaging	0.96
IGL03066:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03160:Hectd4	APN	5	121,397,942 (GRCm39)	missense	probably benign
IGL03181:Hectd4	APN	5	121,492,021 (GRCm39)	missense	possibly damaging	0.91
IGL03265:Hectd4	APN	5	121,398,002 (GRCm39)	splice site	probably benign
IGL03375:Hectd4	APN	5	121,466,445 (GRCm39)	missense	possibly damaging	0.72
Achilles	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
agamemnon	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
clymnestra	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
hector	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
helen	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
Merriwether	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
PIT4466001:Hectd4	UTSW	5	121,471,123 (GRCm39)	critical splice donor site	probably null
R0018:Hectd4	UTSW	5	121,392,242 (GRCm39)	missense	possibly damaging	0.53
R0024:Hectd4	UTSW	5	121,446,639 (GRCm39)	missense	possibly damaging	0.92
R0030:Hectd4	UTSW	5	121,400,651 (GRCm39)	nonsense	probably null
R0080:Hectd4	UTSW	5	121,487,435 (GRCm39)	missense	probably benign	0.18
R0110:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0110:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0115:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0128:Hectd4	UTSW	5	121,487,306 (GRCm39)	missense	possibly damaging	0.86
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0132:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0244:Hectd4	UTSW	5	121,467,668 (GRCm39)	missense	probably benign	0.33
R0281:Hectd4	UTSW	5	121,392,314 (GRCm39)	missense	possibly damaging	0.85
R0329:Hectd4	UTSW	5	121,397,927 (GRCm39)	missense	probably benign
R0410:Hectd4	UTSW	5	121,424,329 (GRCm39)	missense	possibly damaging	0.86
R0422:Hectd4	UTSW	5	121,481,145 (GRCm39)	splice site	probably null
R0442:Hectd4	UTSW	5	121,462,045 (GRCm39)	missense	possibly damaging	0.66
R0449:Hectd4	UTSW	5	121,502,653 (GRCm39)	splice site	probably null
R0469:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0469:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0481:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0510:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0510:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0520:Hectd4	UTSW	5	121,469,770 (GRCm39)	missense	possibly damaging	0.53
R0534:Hectd4	UTSW	5	121,486,539 (GRCm39)	missense	possibly damaging	0.96
R0603:Hectd4	UTSW	5	121,442,400 (GRCm39)	missense	possibly damaging	0.46
R0622:Hectd4	UTSW	5	121,486,688 (GRCm39)	missense	possibly damaging	0.53
R0626:Hectd4	UTSW	5	121,415,887 (GRCm39)	missense	probably benign	0.18
R0708:Hectd4	UTSW	5	121,424,526 (GRCm39)	critical splice donor site	probably null
R0710:Hectd4	UTSW	5	121,474,691 (GRCm39)	missense	probably benign	0.08
R0763:Hectd4	UTSW	5	121,445,096 (GRCm39)	unclassified	probably benign
R0764:Hectd4	UTSW	5	121,424,832 (GRCm39)	missense	possibly damaging	0.46
R1123:Hectd4	UTSW	5	121,424,799 (GRCm39)	missense	probably damaging	0.96
R1129:Hectd4	UTSW	5	121,448,662 (GRCm39)	missense	possibly damaging	0.66
R1204:Hectd4	UTSW	5	121,488,548 (GRCm39)	missense	possibly damaging	0.85
R1237:Hectd4	UTSW	5	121,459,570 (GRCm39)	missense	possibly damaging	0.90
R1257:Hectd4	UTSW	5	121,456,687 (GRCm39)	nonsense	probably null
R1391:Hectd4	UTSW	5	121,491,758 (GRCm39)	missense	possibly damaging	0.96
R1395:Hectd4	UTSW	5	121,466,576 (GRCm39)	critical splice donor site	probably null
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1545:Hectd4	UTSW	5	121,462,019 (GRCm39)	missense	possibly damaging	0.87
R1553:Hectd4	UTSW	5	121,487,322 (GRCm39)	missense	probably benign	0.00
R1572:Hectd4	UTSW	5	121,439,941 (GRCm39)	missense	possibly damaging	0.85
R1662:Hectd4	UTSW	5	121,455,308 (GRCm39)	missense	probably benign	0.01
R1705:Hectd4	UTSW	5	121,436,167 (GRCm39)	missense	probably benign
R1715:Hectd4	UTSW	5	121,482,881 (GRCm39)	missense	possibly damaging	0.85
R1728:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1736:Hectd4	UTSW	5	121,487,593 (GRCm39)	missense	possibly damaging	0.53
R1768:Hectd4	UTSW	5	121,496,366 (GRCm39)	missense	possibly damaging	0.70
R1775:Hectd4	UTSW	5	121,429,254 (GRCm39)	splice site	probably benign
R1784:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1843:Hectd4	UTSW	5	121,435,243 (GRCm39)	missense	possibly damaging	0.53
R1914:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R1915:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R2024:Hectd4	UTSW	5	121,419,981 (GRCm39)	missense	possibly damaging	0.86
R2103:Hectd4	UTSW	5	121,493,692 (GRCm39)	missense	probably benign	0.04
R2108:Hectd4	UTSW	5	121,471,487 (GRCm39)	missense	possibly damaging	0.72
R2124:Hectd4	UTSW	5	121,456,702 (GRCm39)	missense	probably damaging	0.97
R2150:Hectd4	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
R2192:Hectd4	UTSW	5	121,453,206 (GRCm39)	missense	possibly damaging	0.46
R2301:Hectd4	UTSW	5	121,491,600 (GRCm39)	missense	probably benign	0.18
R2324:Hectd4	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
R2331:Hectd4	UTSW	5	121,458,089 (GRCm39)	missense	probably benign	0.05
R2504:Hectd4	UTSW	5	121,402,030 (GRCm39)	missense	possibly damaging	0.73
R2504:Hectd4	UTSW	5	121,358,683 (GRCm39)	missense	unknown
R2904:Hectd4	UTSW	5	121,430,787 (GRCm39)	splice site	probably benign
R3843:Hectd4	UTSW	5	121,397,936 (GRCm39)	missense	possibly damaging	0.72
R3934:Hectd4	UTSW	5	121,458,164 (GRCm39)	critical splice donor site	probably null
R3944:Hectd4	UTSW	5	121,441,588 (GRCm39)	splice site	probably benign
R4133:Hectd4	UTSW	5	121,415,897 (GRCm39)	critical splice donor site	probably null
R4271:Hectd4	UTSW	5	121,358,567 (GRCm39)	small deletion	probably benign
R4413:Hectd4	UTSW	5	121,488,544 (GRCm39)	missense	possibly damaging	0.53
R4456:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R4489:Hectd4	UTSW	5	121,424,320 (GRCm39)	missense	possibly damaging	0.73
R4539:Hectd4	UTSW	5	121,452,970 (GRCm39)	nonsense	probably null
R4564:Hectd4	UTSW	5	121,488,494 (GRCm39)	missense	probably benign	0.33
R4582:Hectd4	UTSW	5	121,424,482 (GRCm39)	missense	possibly damaging	0.53
R4629:Hectd4	UTSW	5	121,435,266 (GRCm39)	missense	probably benign	0.01
R4633:Hectd4	UTSW	5	121,487,279 (GRCm39)	missense	probably benign	0.33
R4643:Hectd4	UTSW	5	121,487,118 (GRCm39)	missense	possibly damaging	0.53
R4679:Hectd4	UTSW	5	121,463,314 (GRCm39)	missense	possibly damaging	0.72
R4681:Hectd4	UTSW	5	121,441,678 (GRCm39)	missense	possibly damaging	0.86
R4734:Hectd4	UTSW	5	121,480,040 (GRCm39)	missense	possibly damaging	0.53
R4739:Hectd4	UTSW	5	121,486,505 (GRCm39)	missense	probably benign
R4781:Hectd4	UTSW	5	121,444,170 (GRCm39)	critical splice donor site	probably null
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4869:Hectd4	UTSW	5	121,460,735 (GRCm39)	missense	possibly damaging	0.46
R4909:Hectd4	UTSW	5	121,401,954 (GRCm39)	missense	probably benign	0.18
R4922:Hectd4	UTSW	5	121,497,378 (GRCm39)	missense	possibly damaging	0.86
R4925:Hectd4	UTSW	5	121,460,753 (GRCm39)	missense	possibly damaging	0.83
R5004:Hectd4	UTSW	5	121,467,628 (GRCm39)	missense	possibly damaging	0.93
R5004:Hectd4	UTSW	5	121,466,262 (GRCm39)	splice site	probably null
R5129:Hectd4	UTSW	5	121,481,573 (GRCm39)	missense	possibly damaging	0.87
R5217:Hectd4	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
R5267:Hectd4	UTSW	5	121,482,887 (GRCm39)	missense	probably benign	0.28
R5344:Hectd4	UTSW	5	121,481,739 (GRCm39)	missense	probably benign	0.28
R5345:Hectd4	UTSW	5	121,402,037 (GRCm39)	missense	possibly damaging	0.85
R5347:Hectd4	UTSW	5	121,442,511 (GRCm39)	missense	probably benign	0.33
R5360:Hectd4	UTSW	5	121,453,464 (GRCm39)	missense	possibly damaging	0.90
R5363:Hectd4	UTSW	5	121,448,666 (GRCm39)	missense	probably benign	0.04
R5445:Hectd4	UTSW	5	121,404,337 (GRCm39)	missense	probably benign	0.00
R5479:Hectd4	UTSW	5	121,445,011 (GRCm39)	missense	probably benign
R5507:Hectd4	UTSW	5	121,419,164 (GRCm39)	missense	unknown
R5552:Hectd4	UTSW	5	121,480,914 (GRCm39)	missense	possibly damaging	0.96
R5691:Hectd4	UTSW	5	121,486,878 (GRCm39)	missense	possibly damaging	0.85
R5745:Hectd4	UTSW	5	121,491,565 (GRCm39)	missense	possibly damaging	0.96
R5757:Hectd4	UTSW	5	121,486,682 (GRCm39)	missense	possibly damaging	0.72
R5845:Hectd4	UTSW	5	121,445,587 (GRCm39)	critical splice donor site	probably null
R5869:Hectd4	UTSW	5	121,481,288 (GRCm39)	critical splice donor site	probably null
R5913:Hectd4	UTSW	5	121,462,037 (GRCm39)	missense	possibly damaging	0.83
R5920:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R5943:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.01
R6219:Hectd4	UTSW	5	121,446,941 (GRCm39)	missense	possibly damaging	0.92
R6250:Hectd4	UTSW	5	121,477,561 (GRCm39)	missense	possibly damaging	0.85
R6301:Hectd4	UTSW	5	121,392,283 (GRCm39)	missense	possibly damaging	0.91
R6428:Hectd4	UTSW	5	121,488,508 (GRCm39)	missense	possibly damaging	0.53
R6446:Hectd4	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
R6453:Hectd4	UTSW	5	121,488,655 (GRCm39)	missense	probably damaging	1.00
R6513:Hectd4	UTSW	5	121,494,259 (GRCm39)	splice site	probably null
R6540:Hectd4	UTSW	5	121,441,634 (GRCm39)	missense	probably benign	0.33
R6706:Hectd4	UTSW	5	121,458,147 (GRCm39)	missense	possibly damaging	0.92
R6720:Hectd4	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
R6736:Hectd4	UTSW	5	121,415,788 (GRCm39)	missense	possibly damaging	0.86
R6776:Hectd4	UTSW	5	121,491,574 (GRCm39)	missense	possibly damaging	0.85
R7033:Hectd4	UTSW	5	121,502,631 (GRCm39)	missense	possibly damaging	0.86
R7038:Hectd4	UTSW	5	121,437,660 (GRCm39)	missense	possibly damaging	0.90
R7175:Hectd4	UTSW	5	121,411,692 (GRCm39)	missense	possibly damaging	0.85
R7180:Hectd4	UTSW	5	121,446,405 (GRCm39)	missense	probably benign	0.01
R7234:Hectd4	UTSW	5	121,467,136 (GRCm39)	missense	possibly damaging	0.53
R7253:Hectd4	UTSW	5	121,452,944 (GRCm39)	missense	possibly damaging	0.66
R7349:Hectd4	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
R7450:Hectd4	UTSW	5	121,419,995 (GRCm39)	missense	probably benign	0.00
R7467:Hectd4	UTSW	5	121,462,024 (GRCm39)	missense	possibly damaging	0.66
R7475:Hectd4	UTSW	5	121,496,196 (GRCm39)	splice site	probably null
R7482:Hectd4	UTSW	5	121,501,941 (GRCm39)	missense	possibly damaging	0.71
R7512:Hectd4	UTSW	5	121,435,172 (GRCm39)	missense	possibly damaging	0.72
R7525:Hectd4	UTSW	5	121,481,728 (GRCm39)	missense	possibly damaging	0.70
R7559:Hectd4	UTSW	5	121,453,573 (GRCm39)	splice site	probably null
R7560:Hectd4	UTSW	5	121,392,405 (GRCm39)	missense	possibly damaging	0.53
R7561:Hectd4	UTSW	5	121,429,288 (GRCm39)	missense	possibly damaging	0.91
R7576:Hectd4	UTSW	5	121,487,522 (GRCm39)	missense	possibly damaging	0.91
R7584:Hectd4	UTSW	5	121,456,798 (GRCm39)	missense	possibly damaging	0.83
R7648:Hectd4	UTSW	5	121,392,434 (GRCm39)	missense	possibly damaging	0.73
R7663:Hectd4	UTSW	5	121,462,094 (GRCm39)	missense	probably benign	0.06
R7692:Hectd4	UTSW	5	121,459,627 (GRCm39)	missense	possibly damaging	0.46
R7725:Hectd4	UTSW	5	121,358,680 (GRCm39)	missense	unknown
R7731:Hectd4	UTSW	5	121,445,077 (GRCm39)	missense	probably benign	0.00
R7732:Hectd4	UTSW	5	121,474,692 (GRCm39)	missense	probably benign	0.14
R7782:Hectd4	UTSW	5	121,443,784 (GRCm39)	missense	possibly damaging	0.53
R7854:Hectd4	UTSW	5	121,467,631 (GRCm39)	missense	probably benign	0.27
R7898:Hectd4	UTSW	5	121,469,880 (GRCm39)	missense	probably benign	0.18
R7910:Hectd4	UTSW	5	121,392,291 (GRCm39)	missense	possibly damaging	0.86
R7962:Hectd4	UTSW	5	121,448,692 (GRCm39)	missense	probably damaging	0.98
R8003:Hectd4	UTSW	5	121,477,581 (GRCm39)	missense	possibly damaging	0.85
R8098:Hectd4	UTSW	5	121,459,461 (GRCm39)	missense	possibly damaging	0.46
R8110:Hectd4	UTSW	5	121,471,012 (GRCm39)	missense	possibly damaging	0.96
R8118:Hectd4	UTSW	5	121,424,439 (GRCm39)	missense	probably benign	0.33
R8171:Hectd4	UTSW	5	121,456,819 (GRCm39)	missense	possibly damaging	0.82
R8234:Hectd4	UTSW	5	121,477,607 (GRCm39)	missense	possibly damaging	0.72
R8289:Hectd4	UTSW	5	121,404,424 (GRCm39)	missense	possibly damaging	0.53
R8292:Hectd4	UTSW	5	121,455,288 (GRCm39)	missense	possibly damaging	0.66
R8348:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8397:Hectd4	UTSW	5	121,397,957 (GRCm39)	missense	probably damaging	0.98
R8436:Hectd4	UTSW	5	121,446,421 (GRCm39)	missense	possibly damaging	0.90
R8436:Hectd4	UTSW	5	121,481,210 (GRCm39)	missense	probably benign	0.00
R8443:Hectd4	UTSW	5	121,467,172 (GRCm39)	missense	possibly damaging	0.72
R8448:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8516:Hectd4	UTSW	5	121,487,073 (GRCm39)	missense	possibly damaging	0.53
R8519:Hectd4	UTSW	5	121,442,489 (GRCm39)	nonsense	probably null
R8553:Hectd4	UTSW	5	121,491,661 (GRCm39)	missense	possibly damaging	0.73
R8557:Hectd4	UTSW	5	121,448,714 (GRCm39)	missense	possibly damaging	0.66
R8725:Hectd4	UTSW	5	121,488,557 (GRCm39)	missense	probably damaging	1.00
R8751:Hectd4	UTSW	5	121,501,838 (GRCm39)	nonsense	probably null
R8769:Hectd4	UTSW	5	121,419,936 (GRCm39)	missense	possibly damaging	0.53
R8803:Hectd4	UTSW	5	121,461,994 (GRCm39)	missense	probably benign	0.01
R8887:Hectd4	UTSW	5	121,433,541 (GRCm39)	missense	probably benign	0.44
R8982:Hectd4	UTSW	5	121,466,305 (GRCm39)	missense	probably benign	0.02
R8988:Hectd4	UTSW	5	121,415,819 (GRCm39)	missense	possibly damaging	0.86
R8991:Hectd4	UTSW	5	121,496,347 (GRCm39)	missense	probably benign	0.33
R8994:Hectd4	UTSW	5	121,441,629 (GRCm39)	missense	probably benign	0.33
R8995:Hectd4	UTSW	5	121,392,422 (GRCm39)	missense	possibly damaging	0.96
R9049:Hectd4	UTSW	5	121,451,955 (GRCm39)	missense	possibly damaging	0.92
R9093:Hectd4	UTSW	5	121,411,677 (GRCm39)	missense	probably benign	0.14
R9106:Hectd4	UTSW	5	121,467,619 (GRCm39)	missense	possibly damaging	0.53
R9137:Hectd4	UTSW	5	121,496,238 (GRCm39)	missense	possibly damaging	0.53
R9146:Hectd4	UTSW	5	121,487,097 (GRCm39)	missense	probably benign	0.33
R9154:Hectd4	UTSW	5	121,391,967 (GRCm39)	missense
R9162:Hectd4	UTSW	5	121,445,042 (GRCm39)	missense	possibly damaging	0.66
R9166:Hectd4	UTSW	5	121,446,690 (GRCm39)	missense	probably damaging	0.96
R9183:Hectd4	UTSW	5	121,437,551 (GRCm39)	missense	possibly damaging	0.51
R9207:Hectd4	UTSW	5	121,433,496 (GRCm39)	missense	possibly damaging	0.86
R9291:Hectd4	UTSW	5	121,487,028 (GRCm39)	missense	probably benign	0.14
R9300:Hectd4	UTSW	5	121,486,952 (GRCm39)	missense	probably benign	0.33
R9314:Hectd4	UTSW	5	121,437,708 (GRCm39)	critical splice donor site	probably null
R9381:Hectd4	UTSW	5	121,472,492 (GRCm39)	missense	possibly damaging	0.53
R9432:Hectd4	UTSW	5	121,460,864 (GRCm39)	missense	probably benign	0.01
R9491:Hectd4	UTSW	5	121,452,981 (GRCm39)	missense	probably damaging	0.97
R9532:Hectd4	UTSW	5	121,502,616 (GRCm39)	missense	probably benign	0.00
R9557:Hectd4	UTSW	5	121,459,617 (GRCm39)	missense	possibly damaging	0.66
R9561:Hectd4	UTSW	5	121,472,532 (GRCm39)	missense	possibly damaging	0.53
R9593:Hectd4	UTSW	5	121,424,844 (GRCm39)	nonsense	probably null
R9704:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9705:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9712:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9713:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9726:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9732:Hectd4	UTSW	5	121,392,254 (GRCm39)	nonsense	probably null
R9750:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9752:Hectd4	UTSW	5	121,472,415 (GRCm39)	missense	possibly damaging	0.85
R9752:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9772:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
X0026:Hectd4	UTSW	5	121,487,700 (GRCm39)	missense	probably benign	0.04
X0027:Hectd4	UTSW	5	121,459,467 (GRCm39)	missense	probably benign	0.27
Z1088:Hectd4	UTSW	5	121,433,566 (GRCm39)	splice site	probably null
Z1177:Hectd4	UTSW	5	121,496,383 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AGCGTCTTGCATGGGTTACAGC -3'
(R):5'- ACTGGGCATAAGTCATCGGCAC -3'

Sequencing Primer
(F):5'- CCAGATGATGAAGTGTGCCC -3'
(R):5'- GCACCACCTTTAGAAGGCTG -3'

Posted On

2013-07-11