Mutagenetix > Incidental Mutation

Incidental Mutation 'R9300:Rif1'

704830

Institutional Source

Beutler Lab

Gene Symbol

Rif1

Ensembl Gene

ENSMUSG00000036202

Gene Name

replication timing regulatory factor 1

Synonyms

6530403D07Rik, 5730435J01Rik, D2Ertd145e

MMRRC Submission

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9300 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

52072832-52122383 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 52111139 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Leucine at position 1535 (S1535L)

Ref Sequence

ENSEMBL: ENSMUSP00000108313 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000112693]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000112693
AA Change: S1535L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108313
Gene: ENSMUSG00000036202
AA Change: S1535L

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	18	381	1.4e-85	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000125376

Predicted Effect

probably benign
Transcript: ENSMUST00000152178

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality. Mice homozygous for a gene trap allele exhibit embryonic and postnatal lethality, reduced fertility, and decreased cell proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1b	G	A	5: 8,825,614 (GRCm38)	V596I	probably benign	Het
Acad8	A	T	9: 26,977,632 (GRCm38)	H371Q	probably damaging	Het
Actn3	G	T	19: 4,871,628 (GRCm38)	R175S	probably benign	Het
Adamts9	G	T	6: 92,887,390 (GRCm38)	D780E	probably benign	Het
AK157302	C	A	13: 21,495,367 (GRCm38)	T21K	probably benign	Het
Ank3	A	T	10: 69,871,042 (GRCm38)	H329L	unknown	Het
Ankrd36	A	G	11: 5,569,979 (GRCm38)	H18R	possibly damaging	Het
Anks1b	T	A	10: 90,577,104 (GRCm38)	V38D	possibly damaging	Het
Apbb2	A	T	5: 66,313,334 (GRCm38)	L520H	probably damaging	Het
Arid1b	C	A	17: 5,242,999 (GRCm38)	P731T	probably damaging	Het
Atp1a4	A	G	1: 172,239,831 (GRCm38)	F578S	probably damaging	Het
Atp6v0a2	A	T	5: 124,712,248 (GRCm38)	I360F	probably damaging	Het
Bcas3	A	T	11: 85,557,358 (GRCm38)	I600F	probably damaging	Het
C1qtnf7	T	G	5: 43,609,099 (GRCm38)	C20W	possibly damaging	Het
Cadm1	A	T	9: 47,797,523 (GRCm38)	K184*	probably null	Het
Ccdc138	A	T	10: 58,507,626 (GRCm38)	H101L	probably benign	Het
Cep120	T	A	18: 53,719,297 (GRCm38)	N503I	probably damaging	Het
Chil4	T	C	3: 106,202,558 (GRCm38)	T380A	probably benign	Het
Clip2	A	G	5: 134,498,088 (GRCm38)		probably null	Het
Cyp3a11	G	A	5: 145,862,354 (GRCm38)	P341L	possibly damaging	Het
Cyp3a41a	G	C	5: 145,720,096 (GRCm38)		probably benign	Het
Dcaf1	A	G	9: 106,847,843 (GRCm38)	I509M	possibly damaging	Het
Dcaf13	T	C	15: 39,146,707 (GRCm38)	S429P	probably damaging	Het
Eln	A	G	5: 134,729,366 (GRCm38)	V151A	unknown	Het
Endou	T	G	15: 97,713,073 (GRCm38)	M377L	probably benign	Het
Etfa	A	G	9: 55,488,868 (GRCm38)	I138T	probably damaging	Het
Fbxw10	T	A	11: 62,877,283 (GRCm38)	S982T	probably benign	Het
Gm9602	A	T	14: 4,777,288 (GRCm38)	R47*	probably null	Het
Hectd4	G	A	5: 121,348,889 (GRCm38)	V3316I	probably benign	Het
Hif1a	T	C	12: 73,940,528 (GRCm38)	L477S	probably benign	Het
Icam5	G	C	9: 21,035,550 (GRCm38)	G458A	probably benign	Het
Itm2b	A	T	14: 73,366,456 (GRCm38)	D106E	probably benign	Het
Kdm4d	G	A	9: 14,464,040 (GRCm38)	T174M	probably damaging	Het
Kif16b	G	T	2: 142,699,287 (GRCm38)	D1364E	probably benign	Het
Lbhd1	G	A	19: 8,884,186 (GRCm38)	V61M	probably damaging	Het
Lclat1	T	A	17: 73,239,924 (GRCm38)	S279T	probably benign	Het
Lhx4	G	A	1: 155,705,210 (GRCm38)	L190F	probably damaging	Het
Lima1	C	T	15: 99,806,439 (GRCm38)	S283N	probably benign	Het
Magi1	C	A	6: 93,747,228 (GRCm38)	D496Y	probably damaging	Het
Map1a	T	C	2: 121,302,965 (GRCm38)	S1421P	probably damaging	Het
Map3k20	C	T	2: 72,371,913 (GRCm38)	H163Y	probably damaging	Het
Mier3	T	G	13: 111,686,356 (GRCm38)		probably null	Het
Mllt3	G	A	4: 87,774,047 (GRCm38)	Q560*	probably null	Het
Mmp25	T	A	17: 23,632,754 (GRCm38)	D327V	probably benign	Het
Nckap5	G	A	1: 125,981,686 (GRCm38)	Q1603*	probably null	Het
Nell1	G	A	7: 50,062,620 (GRCm38)	V63I	probably benign	Het
Or10al3	C	T	17: 37,700,924 (GRCm38)	P85S	probably damaging	Het
Or2ag2b	A	T	7: 106,818,904 (GRCm38)	I274F	probably benign	Het
Or8a1	A	C	9: 37,730,282 (GRCm38)	S234A	probably benign	Het
Pde12	C	T	14: 26,665,776 (GRCm38)	E526K	probably damaging	Het
Pfn4	T	C	12: 4,775,442 (GRCm38)	V93A	possibly damaging	Het
Poglut2	A	G	1: 44,117,202 (GRCm38)	I75T	possibly damaging	Het
Ppp2r3d	A	G	9: 124,423,977 (GRCm38)	S60P	unknown	Het
Prelp	T	C	1: 133,914,519 (GRCm38)	H296R	probably damaging	Het
Prickle1	T	C	15: 93,500,868 (GRCm38)	E693G	possibly damaging	Het
Rars1	A	G	11: 35,815,661 (GRCm38)	L477P	probably damaging	Het
Rasal1	G	A	5: 120,664,107 (GRCm38)	R328H	probably damaging	Het
Rasef	A	G	4: 73,741,156 (GRCm38)	F220S	probably benign	Het
Rnf17	T	C	14: 56,460,038 (GRCm38)	I463T	possibly damaging	Het
Ryr3	A	T	2: 112,860,350 (GRCm38)	D1138E	probably benign	Het
Scn9a	T	A	2: 66,504,892 (GRCm38)	Y1251F	probably benign	Het
Sdk2	A	G	11: 113,825,030 (GRCm38)	W1568R	possibly damaging	Het
Senp6	A	G	9: 80,142,151 (GRCm38)	E1019G	probably damaging	Het
Shh	A	G	5: 28,458,463 (GRCm38)	Y236H	probably damaging	Het
Sin3a	A	G	9: 57,107,460 (GRCm38)	D655G	probably damaging	Het
Slc35a4	C	T	18: 36,682,221 (GRCm38)	P35S	probably damaging	Het
Slc35g1	T	C	19: 38,396,185 (GRCm38)	L24P	probably benign	Het
Slc39a4	G	T	15: 76,614,559 (GRCm38)	Q312K	probably damaging	Het
Slc6a5	A	T	7: 49,951,427 (GRCm38)	I659F	probably damaging	Het
Slc7a4	T	C	16: 17,574,535 (GRCm38)	Y345C	probably benign	Het
Slco6c1	G	T	1: 97,066,084 (GRCm38)	R645S	probably benign	Het
Soat1	T	C	1: 156,441,353 (GRCm38)	Y209C	probably benign	Het
Tbc1d9b	T	C	11: 50,163,887 (GRCm38)	S953P	probably benign	Het
Tgm1	G	A	14: 55,704,846 (GRCm38)	H683Y	probably benign	Het
Tmem98	G	A	11: 80,817,606 (GRCm38)	R134Q	probably damaging	Het
Trpm3	A	C	19: 22,978,381 (GRCm38)	M1107L	possibly damaging	Het
Vmn1r11	G	A	6: 57,137,887 (GRCm38)	V179I	probably benign	Het
Vmn1r119	G	T	7: 21,011,713 (GRCm38)	T248K	probably damaging	Het
Vmn1r236	T	A	17: 21,286,683 (GRCm38)	I21N	possibly damaging	Het
Vmn2r24	A	G	6: 123,816,071 (GRCm38)	T786A	possibly damaging	Het
Vstm5	A	T	9: 15,257,289 (GRCm38)	I50F	probably damaging	Het
Wdr1	T	C	5: 38,527,912 (GRCm38)	D572G	probably damaging	Het
Xaf1	T	C	11: 72,301,691 (GRCm38)	F5S	probably benign	Het
Zan	A	T	5: 137,470,257 (GRCm38)		probably null	Het
Zfp697	T	C	3: 98,427,663 (GRCm38)	V248A	possibly damaging	Het
Zftraf1	C	A	15: 76,646,341 (GRCm38)	V260L	probably benign	Het
Zkscan3	T	A	13: 21,393,497 (GRCm38)	T249S	unknown	Het
Znfx1	A	G	2: 167,055,940 (GRCm38)	Y355H	probably damaging	Het

Other mutations in Rif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Rif1	APN	2	52,121,007 (GRCm38)	missense	probably damaging	0.96
IGL00711:Rif1	APN	2	52,111,070 (GRCm38)	missense	probably benign	0.00
IGL00721:Rif1	APN	2	52,119,117 (GRCm38)	missense	probably damaging	1.00
IGL01085:Rif1	APN	2	52,085,140 (GRCm38)	missense	possibly damaging	0.71
IGL01093:Rif1	APN	2	52,095,948 (GRCm38)	missense	probably damaging	1.00
IGL01107:Rif1	APN	2	52,111,303 (GRCm38)	missense	probably benign	0.00
IGL01138:Rif1	APN	2	52,111,522 (GRCm38)	missense	probably damaging	1.00
IGL01844:Rif1	APN	2	52,112,543 (GRCm38)	missense	probably benign	0.07
IGL02441:Rif1	APN	2	52,105,515 (GRCm38)	missense	probably benign	0.00
IGL02448:Rif1	APN	2	52,116,696 (GRCm38)	missense	probably damaging	0.99
IGL02563:Rif1	APN	2	52,077,065 (GRCm38)	missense	probably damaging	1.00
IGL02704:Rif1	APN	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
IGL02946:Rif1	APN	2	52,110,125 (GRCm38)	nonsense	probably null
IGL03060:Rif1	APN	2	52,112,137 (GRCm38)	missense	probably damaging	0.97
IGL03206:Rif1	APN	2	52,103,622 (GRCm38)	missense	probably damaging	1.00
IGL03263:Rif1	APN	2	52,090,261 (GRCm38)	missense	probably damaging	0.99
IGL03267:Rif1	APN	2	52,076,988 (GRCm38)	missense	possibly damaging	0.94
IGL03280:Rif1	APN	2	52,112,599 (GRCm38)	missense	probably benign	0.32
hifi	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
nietzsche	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
PIT4305001:Rif1	UTSW	2	52,111,958 (GRCm38)	missense
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0104:Rif1	UTSW	2	52,110,092 (GRCm38)	missense	possibly damaging	0.77
R0268:Rif1	UTSW	2	52,090,286 (GRCm38)	critical splice donor site	probably null
R0276:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0278:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0288:Rif1	UTSW	2	52,110,013 (GRCm38)	missense	probably damaging	1.00
R0314:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0345:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0346:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0383:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R0384:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0387:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0388:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0456:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0477:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0505:Rif1	UTSW	2	52,110,737 (GRCm38)	missense	probably damaging	0.99
R0510:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0511:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0633:Rif1	UTSW	2	52,112,563 (GRCm38)	missense	probably benign	0.00
R0637:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0638:Rif1	UTSW	2	52,111,588 (GRCm38)	missense	probably benign	0.12
R0666:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0675:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0707:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0726:Rif1	UTSW	2	52,110,353 (GRCm38)	missense	possibly damaging	0.52
R0743:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0744:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0938:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0939:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0940:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0941:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0942:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0943:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1006:Rif1	UTSW	2	52,085,029 (GRCm38)	missense	probably damaging	0.99
R1052:Rif1	UTSW	2	52,111,562 (GRCm38)	missense	probably benign	0.01
R1061:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1175:Rif1	UTSW	2	52,107,628 (GRCm38)	unclassified	probably benign
R1183:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1184:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1271:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1332:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1336:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1351:Rif1	UTSW	2	52,111,555 (GRCm38)	missense	possibly damaging	0.74
R1517:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1527:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1560:Rif1	UTSW	2	52,111,131 (GRCm38)	missense	probably damaging	1.00
R1563:Rif1	UTSW	2	52,073,223 (GRCm38)	missense	probably damaging	0.99
R1571:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1625:Rif1	UTSW	2	52,103,640 (GRCm38)	missense	probably benign	0.25
R1679:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1689:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1731:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1744:Rif1	UTSW	2	52,112,392 (GRCm38)	missense	possibly damaging	0.56
R1746:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1748:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1831:Rif1	UTSW	2	52,078,495 (GRCm38)	nonsense	probably null
R1902:Rif1	UTSW	2	52,116,673 (GRCm38)	missense	possibly damaging	0.93
R1964:Rif1	UTSW	2	52,098,409 (GRCm38)	missense	probably benign	0.01
R1978:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2000:Rif1	UTSW	2	52,081,298 (GRCm38)	missense	probably damaging	0.99
R2030:Rif1	UTSW	2	52,092,346 (GRCm38)	missense	probably damaging	1.00
R2056:Rif1	UTSW	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
R2106:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2109:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2125:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2126:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2145:Rif1	UTSW	2	52,111,400 (GRCm38)	missense	possibly damaging	0.63
R2152:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2153:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2213:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2327:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2513:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2516:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2520:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2905:Rif1	UTSW	2	52,098,504 (GRCm38)	missense	probably damaging	0.99
R3005:Rif1	UTSW	2	52,082,764 (GRCm38)	missense	probably damaging	1.00
R3155:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3156:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3429:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3707:Rif1	UTSW	2	52,093,580 (GRCm38)	missense	probably damaging	1.00
R3907:Rif1	UTSW	2	52,112,545 (GRCm38)	missense	probably benign	0.03
R3978:Rif1	UTSW	2	52,116,747 (GRCm38)	critical splice donor site	probably null
R4023:Rif1	UTSW	2	52,121,087 (GRCm38)	missense	probably benign	0.01
R4052:Rif1	UTSW	2	52,098,471 (GRCm38)	nonsense	probably null
R4668:Rif1	UTSW	2	52,111,952 (GRCm38)	missense	probably benign	0.01
R4674:Rif1	UTSW	2	52,106,942 (GRCm38)	missense	probably null	1.00
R4715:Rif1	UTSW	2	52,073,139 (GRCm38)	utr 5 prime	probably benign
R4766:Rif1	UTSW	2	52,098,934 (GRCm38)	missense	probably damaging	1.00
R4783:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4785:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4869:Rif1	UTSW	2	52,093,611 (GRCm38)	intron	probably benign
R4911:Rif1	UTSW	2	52,110,518 (GRCm38)	missense	probably damaging	0.98
R4951:Rif1	UTSW	2	52,084,986 (GRCm38)	splice site	probably null
R5044:Rif1	UTSW	2	52,109,928 (GRCm38)	missense	probably damaging	0.99
R5088:Rif1	UTSW	2	52,092,295 (GRCm38)	missense	possibly damaging	0.91
R5151:Rif1	UTSW	2	52,120,309 (GRCm38)	missense	probably damaging	1.00
R5187:Rif1	UTSW	2	52,081,289 (GRCm38)	missense	probably damaging	1.00
R5222:Rif1	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
R5243:Rif1	UTSW	2	52,111,824 (GRCm38)	missense	possibly damaging	0.86
R5436:Rif1	UTSW	2	52,120,971 (GRCm38)	intron	probably benign
R5476:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
R5496:Rif1	UTSW	2	52,098,916 (GRCm38)	missense	probably damaging	1.00
R5641:Rif1	UTSW	2	52,121,158 (GRCm38)	missense	possibly damaging	0.80
R5883:Rif1	UTSW	2	52,105,639 (GRCm38)	critical splice donor site	probably null
R5987:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5990:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5992:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6019:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6020:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6255:Rif1	UTSW	2	52,085,053 (GRCm38)	missense	probably damaging	1.00
R6342:Rif1	UTSW	2	52,119,156 (GRCm38)	missense	probably damaging	0.97
R6364:Rif1	UTSW	2	52,107,669 (GRCm38)	missense	probably damaging	0.97
R6747:Rif1	UTSW	2	52,078,263 (GRCm38)	splice site	probably null
R6928:Rif1	UTSW	2	52,095,961 (GRCm38)	missense	probably damaging	1.00
R6954:Rif1	UTSW	2	52,112,691 (GRCm38)	missense	probably benign	0.00
R7003:Rif1	UTSW	2	52,076,989 (GRCm38)	missense	probably benign	0.06
R7310:Rif1	UTSW	2	52,105,619 (GRCm38)	missense	probably benign	0.12
R7549:Rif1	UTSW	2	52,078,507 (GRCm38)	missense	possibly damaging	0.52
R7603:Rif1	UTSW	2	52,076,175 (GRCm38)	missense	probably damaging	1.00
R7673:Rif1	UTSW	2	52,088,654 (GRCm38)	missense	probably damaging	1.00
R7741:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R7777:Rif1	UTSW	2	52,116,356 (GRCm38)	missense	probably benign	0.00
R7910:Rif1	UTSW	2	52,078,387 (GRCm38)	nonsense	probably null
R7962:Rif1	UTSW	2	52,074,276 (GRCm38)	missense	probably damaging	1.00
R8264:Rif1	UTSW	2	52,090,278 (GRCm38)	missense	noncoding transcript
R8390:Rif1	UTSW	2	52,110,923 (GRCm38)	missense	probably damaging	1.00
R8479:Rif1	UTSW	2	52,112,551 (GRCm38)	missense	possibly damaging	0.52
R8490:Rif1	UTSW	2	52,110,999 (GRCm38)	missense	probably damaging	0.96
R8762:Rif1	UTSW	2	52,111,730 (GRCm38)	missense
R8785:Rif1	UTSW	2	52,110,481 (GRCm38)	missense	probably benign	0.06
R8890:Rif1	UTSW	2	52,098,863 (GRCm38)	missense	probably damaging	0.99
R9081:Rif1	UTSW	2	52,110,977 (GRCm38)	missense	probably damaging	0.99
R9225:Rif1	UTSW	2	52,111,850 (GRCm38)	missense	probably benign	0.22
R9284:Rif1	UTSW	2	52,108,552 (GRCm38)	missense	probably benign	0.00
R9366:Rif1	UTSW	2	52,120,344 (GRCm38)	missense
R9477:Rif1	UTSW	2	52,111,330 (GRCm38)	missense	probably benign	0.02
R9522:Rif1	UTSW	2	52,081,299 (GRCm38)	missense	probably damaging	1.00
R9573:Rif1	UTSW	2	52,110,454 (GRCm38)	missense	probably benign	0.29
R9630:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
X0064:Rif1	UTSW	2	52,094,633 (GRCm38)	missense	probably damaging	0.96
X0064:Rif1	UTSW	2	52,074,315 (GRCm38)	missense	probably benign	0.00
Z1177:Rif1	UTSW	2	52,088,648 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTTACCTGAGAGAACTTCAGGGG -3'
(R):5'- AGCCGCTGCATTTACATCAAC -3'

Sequencing Primer
(F):5'- GGAACCCAGTGTTAATGCTGAAATTG -3'
(R):5'- GCTGCATTTACATCAACATCAGG -3'

Posted On

2022-03-25