Mutagenetix > Incidental Mutation

Incidental Mutation 'R2125:Rif1'

229722

Institutional Source

Beutler Lab

Gene Symbol

Rif1

Ensembl Gene

ENSMUSG00000036202

Gene Name

replication timing regulatory factor 1

Synonyms

6530403D07Rik, 5730435J01Rik, D2Ertd145e

MMRRC Submission

040128-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2125 (G1)

Quality Score

217

Status

Not validated

Chromosome

Chromosomal Location

52072832-52122383 bp(+) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

GCCACCA to GCCA at 52110324 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069794] [ENSMUST00000112693]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000069794
AA Change: 1264

SMART Domains

Protein: ENSMUSP00000064155
Gene: ENSMUSG00000036202
AA Change: 1264

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	22	368	3.3e-78	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112693
AA Change: P1264

SMART Domains

Protein: ENSMUSP00000108313
Gene: ENSMUSG00000036202
AA Change: P1264

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	18	381	1.4e-85	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000125322

Predicted Effect

probably benign
Transcript: ENSMUST00000125376

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145130

Predicted Effect

probably benign
Transcript: ENSMUST00000152178

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality. Mice homozygous for a gene trap allele exhibit embryonic and postnatal lethality, reduced fertility, and decreased cell proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9530053A07Rik	A	T	7: 28,158,022 (GRCm38)	Y2265F	probably benign	Het
Aadac	A	G	3: 60,039,645 (GRCm38)	T255A	possibly damaging	Het
Abcb10	A	C	8: 123,965,092 (GRCm38)	V378G	probably benign	Het
Ackr2	C	T	9: 121,908,786 (GRCm38)	R76*	probably null	Het
Acly	T	C	11: 100,523,496 (GRCm38)	T35A	probably benign	Het
Acp7	A	C	7: 28,629,549 (GRCm38)	F69V	probably damaging	Het
Acsl3	T	A	1: 78,681,961 (GRCm38)	I110N	probably damaging	Het
Adam5	C	T	8: 24,815,118 (GRCm38)	V107M	probably damaging	Het
Adck2	T	C	6: 39,575,142 (GRCm38)	V281A	probably benign	Het
Adgrv1	A	G	13: 81,419,535 (GRCm38)	V5173A	probably benign	Het
Adgrv1	A	T	13: 81,419,950 (GRCm38)	S5035T	probably benign	Het
AI314180	T	C	4: 58,833,978 (GRCm38)	H834R	probably benign	Het
Arl10	T	A	13: 54,579,124 (GRCm38)		probably null	Het
B3gnt3	A	G	8: 71,693,358 (GRCm38)	W176R	probably damaging	Het
B4galt4	T	C	16: 38,765,938 (GRCm38)	I3T	probably damaging	Het
Casr	T	C	16: 36,495,252 (GRCm38)	I819V	possibly damaging	Het
Cdh1	T	C	8: 106,656,840 (GRCm38)	V237A	probably damaging	Het
Col22a1	G	A	15: 71,848,577 (GRCm38)	R605C	unknown	Het
Crlf3	A	G	11: 80,059,255 (GRCm38)	V183A	probably benign	Het
Crtac1	C	A	19: 42,323,732 (GRCm38)	V181L	probably damaging	Het
Dclk2	C	T	3: 86,805,639 (GRCm38)	R503Q	possibly damaging	Het
Dnah12	A	T	14: 26,724,458 (GRCm38)	R725*	probably null	Het
Dnhd1	A	T	7: 105,677,971 (GRCm38)	H709L	probably benign	Het
Dopey1	A	G	9: 86,521,046 (GRCm38)	Y1431C	probably damaging	Het
Eif2ak4	A	T	2: 118,422,123 (GRCm38)	H392L	probably benign	Het
Entpd3	T	C	9: 120,555,654 (GRCm38)	I99T	probably damaging	Het
Epha6	T	C	16: 59,682,688 (GRCm38)	D952G	probably damaging	Het
Exoc6	T	C	19: 37,590,941 (GRCm38)	L429P	probably damaging	Het
F13a1	A	G	13: 36,892,841 (GRCm38)	S625P	probably benign	Het
Fcho2	T	C	13: 98,775,898 (GRCm38)	N240S	possibly damaging	Het
Fcrl6	C	T	1: 172,599,248 (GRCm38)	V44M	probably benign	Het
Fmo6	A	T	1: 162,929,958 (GRCm38)	M82K	possibly damaging	Het
Gabrr2	T	G	4: 33,095,548 (GRCm38)	I479R	probably damaging	Het
Gm14409	C	A	2: 177,265,402 (GRCm38)	C101F	probably damaging	Het
Gnl2	T	A	4: 125,053,485 (GRCm38)	F633L	probably benign	Het
Greb1l	C	T	18: 10,511,422 (GRCm38)	S648F	probably damaging	Het
Gsap	T	A	5: 21,242,813 (GRCm38)	C290S	probably damaging	Het
Gusb	A	G	5: 129,999,447 (GRCm38)	V268A	probably benign	Het
H2-D1	G	A	17: 35,264,115 (GRCm38)		probably null	Het
Hebp2	T	A	10: 18,541,260 (GRCm38)	E164D	probably benign	Het
Higd1a	A	T	9: 121,850,247 (GRCm38)	I58N	probably damaging	Het
Hoxc13	G	T	15: 102,927,223 (GRCm38)	R262L	probably damaging	Het
Ifih1	A	G	2: 62,623,467 (GRCm38)	V218A	probably benign	Het
Impg2	T	A	16: 56,265,064 (GRCm38)	Y1045N	probably damaging	Het
Kcnk1	A	G	8: 125,995,656 (GRCm38)	E66G	probably damaging	Het
Klkb1	A	G	8: 45,275,504 (GRCm38)	V406A	possibly damaging	Het
Klra10	G	A	6: 130,279,278 (GRCm38)	R138W	probably damaging	Het
Lama2	A	C	10: 27,044,453 (GRCm38)	Y193*	probably null	Het
Larp7	T	C	3: 127,543,130 (GRCm38)	T428A	probably benign	Het
Lipg	T	C	18: 74,945,885 (GRCm38)	N432S	probably benign	Het
Loxl1	T	C	9: 58,293,712 (GRCm38)	D489G	probably damaging	Het
Lrguk	A	G	6: 34,092,902 (GRCm38)	K571E	probably benign	Het
Map3k13	C	T	16: 21,892,144 (GRCm38)	T59I	probably benign	Het
Mertk	T	A	2: 128,762,138 (GRCm38)	D397E	probably benign	Het
Mgat3	G	A	15: 80,211,886 (GRCm38)	V305I	probably benign	Het
Mkl2	T	C	16: 13,400,804 (GRCm38)	V449A	possibly damaging	Het
Mybpc1	G	A	10: 88,573,437 (GRCm38)	Q66*	probably null	Het
Myo3a	A	G	2: 22,578,174 (GRCm38)	D480G	probably benign	Het
Ndufaf3	C	T	9: 108,566,737 (GRCm38)	R31Q	probably benign	Het
Neb	T	C	2: 52,310,638 (GRCm38)	Y343C	probably damaging	Het
Nrxn3	A	G	12: 89,260,520 (GRCm38)		probably null	Het
Olfr1090	A	T	2: 86,754,452 (GRCm38)	N95K	probably benign	Het
Olfr1257	A	G	2: 89,881,638 (GRCm38)	M271V	probably benign	Het
Pcsk4	T	C	10: 80,323,879 (GRCm38)	M379V	probably benign	Het
Pdzd2	A	T	15: 12,373,590 (GRCm38)	V2153E	probably benign	Het
Pkd2l1	T	C	19: 44,154,500 (GRCm38)	D427G	possibly damaging	Het
Plcd4	A	G	1: 74,565,152 (GRCm38)	Y763C	probably damaging	Het
Plekhh1	T	C	12: 79,079,000 (GRCm38)	F1270S	probably damaging	Het
Pmfbp1	T	C	8: 109,520,273 (GRCm38)	V259A	probably damaging	Het
Ptprg	T	C	14: 12,179,283 (GRCm38)	S767P	possibly damaging	Het
Rabl3	T	G	16: 37,556,813 (GRCm38)		probably null	Het
Rffl	T	A	11: 82,818,438 (GRCm38)	H53L	probably damaging	Het
Rtl1	C	T	12: 109,593,921 (GRCm38)	V495I	possibly damaging	Het
Scaf11	A	T	15: 96,419,315 (GRCm38)	D789E	possibly damaging	Het
Scn2a	T	A	2: 65,752,079 (GRCm38)	Y1590*	probably null	Het
Siglech	T	A	7: 55,771,686 (GRCm38)	F190I	probably benign	Het
Slc22a22	C	A	15: 57,254,240 (GRCm38)	A302S	probably damaging	Het
Slc46a3	T	A	5: 147,879,144 (GRCm38)	T458S	probably benign	Het
Spta1	G	T	1: 174,208,344 (GRCm38)	R1072L	possibly damaging	Het
Tbx5	A	G	5: 119,836,923 (GRCm38)	T4A	probably benign	Het
Tdrd5	T	C	1: 156,276,573 (GRCm38)	R528G	probably damaging	Het
Tfip11	T	C	5: 112,335,663 (GRCm38)	V648A	possibly damaging	Het
Thumpd3	T	C	6: 113,066,788 (GRCm38)	V388A	probably benign	Het
Tmem131l	C	T	3: 83,942,751 (GRCm38)		probably null	Het
Ttf2	C	A	3: 100,948,193 (GRCm38)	Q895H	possibly damaging	Het
Ttn	A	T	2: 76,890,092 (GRCm38)		probably null	Het
Vmn2r13	A	G	5: 109,158,192 (GRCm38)	S507P	probably benign	Het
Vtn	A	G	11: 78,500,223 (GRCm38)	T210A	probably damaging	Het
Znhit2	A	G	19: 6,062,061 (GRCm38)	T279A	probably benign	Het

Other mutations in Rif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Rif1	APN	2	52,121,007 (GRCm38)	missense	probably damaging	0.96
IGL00711:Rif1	APN	2	52,111,070 (GRCm38)	missense	probably benign	0.00
IGL00721:Rif1	APN	2	52,119,117 (GRCm38)	missense	probably damaging	1.00
IGL01085:Rif1	APN	2	52,085,140 (GRCm38)	missense	possibly damaging	0.71
IGL01093:Rif1	APN	2	52,095,948 (GRCm38)	missense	probably damaging	1.00
IGL01107:Rif1	APN	2	52,111,303 (GRCm38)	missense	probably benign	0.00
IGL01138:Rif1	APN	2	52,111,522 (GRCm38)	missense	probably damaging	1.00
IGL01844:Rif1	APN	2	52,112,543 (GRCm38)	missense	probably benign	0.07
IGL02441:Rif1	APN	2	52,105,515 (GRCm38)	missense	probably benign	0.00
IGL02448:Rif1	APN	2	52,116,696 (GRCm38)	missense	probably damaging	0.99
IGL02563:Rif1	APN	2	52,077,065 (GRCm38)	missense	probably damaging	1.00
IGL02704:Rif1	APN	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
IGL02946:Rif1	APN	2	52,110,125 (GRCm38)	nonsense	probably null
IGL03060:Rif1	APN	2	52,112,137 (GRCm38)	missense	probably damaging	0.97
IGL03206:Rif1	APN	2	52,103,622 (GRCm38)	missense	probably damaging	1.00
IGL03263:Rif1	APN	2	52,090,261 (GRCm38)	missense	probably damaging	0.99
IGL03267:Rif1	APN	2	52,076,988 (GRCm38)	missense	possibly damaging	0.94
IGL03280:Rif1	APN	2	52,112,599 (GRCm38)	missense	probably benign	0.32
hifi	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
nietzsche	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
PIT4305001:Rif1	UTSW	2	52,111,958 (GRCm38)	missense
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0104:Rif1	UTSW	2	52,110,092 (GRCm38)	missense	possibly damaging	0.77
R0268:Rif1	UTSW	2	52,090,286 (GRCm38)	critical splice donor site	probably null
R0276:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0278:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0288:Rif1	UTSW	2	52,110,013 (GRCm38)	missense	probably damaging	1.00
R0314:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0345:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0346:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0383:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R0384:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0387:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0388:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0456:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0477:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0505:Rif1	UTSW	2	52,110,737 (GRCm38)	missense	probably damaging	0.99
R0510:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0511:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0633:Rif1	UTSW	2	52,112,563 (GRCm38)	missense	probably benign	0.00
R0637:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0638:Rif1	UTSW	2	52,111,588 (GRCm38)	missense	probably benign	0.12
R0666:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0675:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0707:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0726:Rif1	UTSW	2	52,110,353 (GRCm38)	missense	possibly damaging	0.52
R0743:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0744:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0938:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0939:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0940:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0941:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0942:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0943:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1006:Rif1	UTSW	2	52,085,029 (GRCm38)	missense	probably damaging	0.99
R1052:Rif1	UTSW	2	52,111,562 (GRCm38)	missense	probably benign	0.01
R1061:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1175:Rif1	UTSW	2	52,107,628 (GRCm38)	unclassified	probably benign
R1183:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1184:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1271:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1332:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1336:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1351:Rif1	UTSW	2	52,111,555 (GRCm38)	missense	possibly damaging	0.74
R1517:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1527:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1560:Rif1	UTSW	2	52,111,131 (GRCm38)	missense	probably damaging	1.00
R1563:Rif1	UTSW	2	52,073,223 (GRCm38)	missense	probably damaging	0.99
R1571:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1625:Rif1	UTSW	2	52,103,640 (GRCm38)	missense	probably benign	0.25
R1679:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1689:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1731:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1744:Rif1	UTSW	2	52,112,392 (GRCm38)	missense	possibly damaging	0.56
R1746:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1748:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1831:Rif1	UTSW	2	52,078,495 (GRCm38)	nonsense	probably null
R1902:Rif1	UTSW	2	52,116,673 (GRCm38)	missense	possibly damaging	0.93
R1964:Rif1	UTSW	2	52,098,409 (GRCm38)	missense	probably benign	0.01
R1978:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2000:Rif1	UTSW	2	52,081,298 (GRCm38)	missense	probably damaging	0.99
R2030:Rif1	UTSW	2	52,092,346 (GRCm38)	missense	probably damaging	1.00
R2056:Rif1	UTSW	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
R2106:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2109:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2126:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2145:Rif1	UTSW	2	52,111,400 (GRCm38)	missense	possibly damaging	0.63
R2152:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2153:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2213:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2327:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2513:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2516:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2520:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2905:Rif1	UTSW	2	52,098,504 (GRCm38)	missense	probably damaging	0.99
R3005:Rif1	UTSW	2	52,082,764 (GRCm38)	missense	probably damaging	1.00
R3155:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3156:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3429:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3707:Rif1	UTSW	2	52,093,580 (GRCm38)	missense	probably damaging	1.00
R3907:Rif1	UTSW	2	52,112,545 (GRCm38)	missense	probably benign	0.03
R3978:Rif1	UTSW	2	52,116,747 (GRCm38)	critical splice donor site	probably null
R4023:Rif1	UTSW	2	52,121,087 (GRCm38)	missense	probably benign	0.01
R4052:Rif1	UTSW	2	52,098,471 (GRCm38)	nonsense	probably null
R4668:Rif1	UTSW	2	52,111,952 (GRCm38)	missense	probably benign	0.01
R4674:Rif1	UTSW	2	52,106,942 (GRCm38)	missense	probably null	1.00
R4715:Rif1	UTSW	2	52,073,139 (GRCm38)	utr 5 prime	probably benign
R4766:Rif1	UTSW	2	52,098,934 (GRCm38)	missense	probably damaging	1.00
R4783:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4785:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4869:Rif1	UTSW	2	52,093,611 (GRCm38)	intron	probably benign
R4911:Rif1	UTSW	2	52,110,518 (GRCm38)	missense	probably damaging	0.98
R4951:Rif1	UTSW	2	52,084,986 (GRCm38)	splice site	probably null
R5044:Rif1	UTSW	2	52,109,928 (GRCm38)	missense	probably damaging	0.99
R5088:Rif1	UTSW	2	52,092,295 (GRCm38)	missense	possibly damaging	0.91
R5151:Rif1	UTSW	2	52,120,309 (GRCm38)	missense	probably damaging	1.00
R5187:Rif1	UTSW	2	52,081,289 (GRCm38)	missense	probably damaging	1.00
R5222:Rif1	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
R5243:Rif1	UTSW	2	52,111,824 (GRCm38)	missense	possibly damaging	0.86
R5436:Rif1	UTSW	2	52,120,971 (GRCm38)	intron	probably benign
R5476:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
R5496:Rif1	UTSW	2	52,098,916 (GRCm38)	missense	probably damaging	1.00
R5641:Rif1	UTSW	2	52,121,158 (GRCm38)	missense	possibly damaging	0.80
R5883:Rif1	UTSW	2	52,105,639 (GRCm38)	critical splice donor site	probably null
R5987:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5990:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5992:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6019:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6020:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6255:Rif1	UTSW	2	52,085,053 (GRCm38)	missense	probably damaging	1.00
R6342:Rif1	UTSW	2	52,119,156 (GRCm38)	missense	probably damaging	0.97
R6364:Rif1	UTSW	2	52,107,669 (GRCm38)	missense	probably damaging	0.97
R6747:Rif1	UTSW	2	52,078,263 (GRCm38)	splice site	probably null
R6928:Rif1	UTSW	2	52,095,961 (GRCm38)	missense	probably damaging	1.00
R6954:Rif1	UTSW	2	52,112,691 (GRCm38)	missense	probably benign	0.00
R7003:Rif1	UTSW	2	52,076,989 (GRCm38)	missense	probably benign	0.06
R7310:Rif1	UTSW	2	52,105,619 (GRCm38)	missense	probably benign	0.12
R7549:Rif1	UTSW	2	52,078,507 (GRCm38)	missense	possibly damaging	0.52
R7603:Rif1	UTSW	2	52,076,175 (GRCm38)	missense	probably damaging	1.00
R7673:Rif1	UTSW	2	52,088,654 (GRCm38)	missense	probably damaging	1.00
R7741:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R7777:Rif1	UTSW	2	52,116,356 (GRCm38)	missense	probably benign	0.00
R7910:Rif1	UTSW	2	52,078,387 (GRCm38)	nonsense	probably null
R7962:Rif1	UTSW	2	52,074,276 (GRCm38)	missense	probably damaging	1.00
R8264:Rif1	UTSW	2	52,090,278 (GRCm38)	missense	noncoding transcript
R8390:Rif1	UTSW	2	52,110,923 (GRCm38)	missense	probably damaging	1.00
R8479:Rif1	UTSW	2	52,112,551 (GRCm38)	missense	possibly damaging	0.52
R8490:Rif1	UTSW	2	52,110,999 (GRCm38)	missense	probably damaging	0.96
R8762:Rif1	UTSW	2	52,111,730 (GRCm38)	missense
R8785:Rif1	UTSW	2	52,110,481 (GRCm38)	missense	probably benign	0.06
R8890:Rif1	UTSW	2	52,098,863 (GRCm38)	missense	probably damaging	0.99
R9081:Rif1	UTSW	2	52,110,977 (GRCm38)	missense	probably damaging	0.99
R9225:Rif1	UTSW	2	52,111,850 (GRCm38)	missense	probably benign	0.22
R9284:Rif1	UTSW	2	52,108,552 (GRCm38)	missense	probably benign	0.00
R9300:Rif1	UTSW	2	52,111,139 (GRCm38)	missense	probably damaging	1.00
R9366:Rif1	UTSW	2	52,120,344 (GRCm38)	missense
R9477:Rif1	UTSW	2	52,111,330 (GRCm38)	missense	probably benign	0.02
R9522:Rif1	UTSW	2	52,081,299 (GRCm38)	missense	probably damaging	1.00
R9573:Rif1	UTSW	2	52,110,454 (GRCm38)	missense	probably benign	0.29
R9630:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
X0064:Rif1	UTSW	2	52,094,633 (GRCm38)	missense	probably damaging	0.96
X0064:Rif1	UTSW	2	52,074,315 (GRCm38)	missense	probably benign	0.00
Z1177:Rif1	UTSW	2	52,088,648 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTTCGGTGGTCAGCAGTAGTTC -3'
(R):5'- TCTGTTGGGCTGAGCAGATC -3'

Sequencing Primer
(F):5'- GCAGTAGTTCAGTTTCTAATGCCAC -3'
(R):5'- AGCAGATCAGGAGAGTTATTTTCG -3'

Posted On

2014-09-17