Mutagenetix > Incidental Mutation

Incidental Mutation 'R2345:Wdr90'

245997

Institutional Source

Beutler Lab

Gene Symbol

Wdr90

Ensembl Gene

ENSMUSG00000073434

Gene Name

WD repeat domain 90

Synonyms

3230401M21Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R2345 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

26063745-26080475 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 26078136 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Asparagine at position 411 (H411N)

Ref Sequence

ENSEMBL: ENSMUSP00000078426 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026828] [ENSMUST00000079461] [ENSMUST00000176923]

AlphaFold

Q6ZPG2

Predicted Effect

probably benign
Transcript: ENSMUST00000026828

SMART Domains

Protein: ENSMUSP00000026828
Gene: ENSMUSG00000025732

Domain	Start	End	E-Value	Type
Pfam:FAM195	59	158	8.3e-33	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000079461
AA Change: H411N

PolyPhen 2 Score 0.051 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000078426
Gene: ENSMUSG00000073434
AA Change: H411N

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	183	5e-26	PFAM
Pfam:DUF667	210	271	3.7e-9	PFAM
low complexity region	381	396	N/A	INTRINSIC
WD40	478	519	5.94e0	SMART
WD40	522	565	3.2e0	SMART
WD40	572	612	3.3e1	SMART
WD40	687	725	1.15e1	SMART
WD40	728	766	5.75e-1	SMART
WD40	768	808	9.24e-4	SMART
WD40	811	850	4.13e0	SMART
WD40	853	892	4.62e-1	SMART
WD40	950	993	1.07e1	SMART
WD40	996	1035	5.75e-1	SMART
WD40	1040	1077	1.58e-2	SMART
WD40	1290	1334	5.23e-3	SMART
WD40	1337	1378	1.27e-1	SMART
WD40	1384	1419	1.83e2	SMART
WD40	1422	1469	3.08e0	SMART
WD40	1472	1509	9.9e0	SMART
WD40	1568	1607	9.02e-7	SMART
WD40	1610	1655	5.75e-1	SMART
WD40	1659	1697	2.98e-1	SMART
WD40	1700	1749	6.14e1	SMART
WD40	1850	1888	1.92e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000176575

Predicted Effect

unknown
Transcript: ENSMUST00000176678
AA Change: H22N

Predicted Effect

probably benign
Transcript: ENSMUST00000176923
AA Change: H393N

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000135420
Gene: ENSMUSG00000073434
AA Change: H393N

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	252	5.1e-14	PFAM
low complexity region	363	378	N/A	INTRINSIC
WD40	460	501	5.94e0	SMART
WD40	504	547	3.2e0	SMART
WD40	554	594	3.3e1	SMART
WD40	669	707	1.15e1	SMART
WD40	710	748	5.75e-1	SMART
WD40	750	790	9.24e-4	SMART
WD40	793	832	4.13e0	SMART
WD40	835	874	4.62e-1	SMART
WD40	932	975	1.07e1	SMART
WD40	978	1017	5.75e-1	SMART
WD40	1022	1059	1.58e-2	SMART
WD40	1272	1316	5.23e-3	SMART
WD40	1319	1360	1.27e-1	SMART
WD40	1366	1401	1.83e2	SMART
WD40	1404	1451	3.08e0	SMART
WD40	1454	1491	9.9e0	SMART
WD40	1550	1589	9.02e-7	SMART
WD40	1592	1637	5.75e-1	SMART
WD40	1641	1679	2.98e-1	SMART
WD40	1682	1731	6.14e1	SMART
WD40	1832	1870	1.92e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000176963

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177031

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180868

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177170

Meta Mutation Damage Score

0.3116

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.2%

Validation Efficiency

100% (30/30)

Allele List at MGI

Other mutations in this stock

Total: 28 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atxn3	A	T	12: 101,914,580 (GRCm39)	M48K	probably damaging	Het
Bnc2	T	C	4: 84,210,740 (GRCm39)	E638G	probably damaging	Het
Cdh15	G	A	8: 123,583,374 (GRCm39)	R59H	probably benign	Het
Ceacam3	T	G	7: 16,888,925 (GRCm39)	D231E	possibly damaging	Het
Ckb	G	A	12: 111,638,238 (GRCm39)	T52I	probably damaging	Het
Elac2	T	C	11: 64,891,900 (GRCm39)	M773T	probably damaging	Het
Fbxw8	A	T	5: 118,203,872 (GRCm39)		probably benign	Het
Hk3	T	C	13: 55,156,806 (GRCm39)	D582G	probably damaging	Het
Htt	T	A	5: 34,983,348 (GRCm39)	N982K	possibly damaging	Het
Jag2	T	C	12: 112,872,684 (GRCm39)	E1190G	probably damaging	Het
Kcnc1	A	G	7: 46,047,370 (GRCm39)	E90G	probably damaging	Het
Kynu	A	C	2: 43,471,397 (GRCm39)	Y71S	probably damaging	Het
Lonrf1	A	T	8: 36,690,016 (GRCm39)		probably null	Het
Mfsd4b3-ps	T	A	10: 39,824,069 (GRCm39)	M64L	probably benign	Het
Nbea	A	T	3: 55,992,700 (GRCm39)	F302Y	probably damaging	Het
Ndst4	T	C	3: 125,501,769 (GRCm39)	S111P	possibly damaging	Het
Nsf	C	T	11: 103,821,578 (GRCm39)	E26K	possibly damaging	Het
Or1f19	G	A	16: 3,411,003 (GRCm39)	V248M	probably damaging	Het
Or5m8	A	G	2: 85,822,166 (GRCm39)	T2A	probably benign	Het
Or8b57	T	G	9: 40,003,849 (GRCm39)	I138L	probably benign	Het
Phf3	G	T	1: 30,844,432 (GRCm39)	S1509*	probably null	Het
Plekhh1	A	T	12: 79,100,421 (GRCm39)	I130F	probably damaging	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Szt2	A	G	4: 118,238,594 (GRCm39)	F1953S	unknown	Het
Togaram1	T	A	12: 65,055,406 (GRCm39)	S1466T	probably benign	Het
Tox2	T	C	2: 163,161,518 (GRCm39)	Y348H	probably damaging	Het
Vmn2r129	T	A	4: 156,689,334 (GRCm39)		noncoding transcript	Het
Yme1l1	C	T	2: 23,084,798 (GRCm39)	T632I	probably damaging	Het

Other mutations in Wdr90

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00418:Wdr90	APN	17	26,068,338 (GRCm39)	missense	probably damaging	1.00
IGL01118:Wdr90	APN	17	26,073,661 (GRCm39)	missense	probably damaging	1.00
IGL01964:Wdr90	APN	17	26,067,383 (GRCm39)	missense	probably benign
IGL02116:Wdr90	APN	17	26,078,466 (GRCm39)	missense	probably benign	0.12
IGL02172:Wdr90	APN	17	26,069,408 (GRCm39)	missense	probably benign	0.22
IGL02716:Wdr90	APN	17	26,076,194 (GRCm39)	missense	probably damaging	0.96
IGL02961:Wdr90	APN	17	26,067,649 (GRCm39)	nonsense	probably null
IGL03229:Wdr90	APN	17	26,064,437 (GRCm39)	splice site	probably benign
IGL03367:Wdr90	APN	17	26,066,765 (GRCm39)	splice site	probably benign
IGL03098:Wdr90	UTSW	17	26,078,961 (GRCm39)	intron	probably benign
R0111:Wdr90	UTSW	17	26,067,418 (GRCm39)	splice site	probably benign
R0454:Wdr90	UTSW	17	26,079,023 (GRCm39)	missense	probably damaging	0.96
R0457:Wdr90	UTSW	17	26,079,459 (GRCm39)	missense	probably benign	0.00
R0488:Wdr90	UTSW	17	26,067,591 (GRCm39)	missense	probably damaging	1.00
R0622:Wdr90	UTSW	17	26,074,632 (GRCm39)	missense	probably damaging	1.00
R0671:Wdr90	UTSW	17	26,065,367 (GRCm39)	missense	probably benign	0.04
R0799:Wdr90	UTSW	17	26,079,104 (GRCm39)	missense	probably benign	0.38
R1177:Wdr90	UTSW	17	26,065,028 (GRCm39)	missense	possibly damaging	0.85
R1460:Wdr90	UTSW	17	26,079,422 (GRCm39)	missense	possibly damaging	0.82
R1468:Wdr90	UTSW	17	26,073,027 (GRCm39)	missense	probably damaging	1.00
R1468:Wdr90	UTSW	17	26,073,027 (GRCm39)	missense	probably damaging	1.00
R1544:Wdr90	UTSW	17	26,068,284 (GRCm39)	missense	possibly damaging	0.69
R2057:Wdr90	UTSW	17	26,074,173 (GRCm39)	missense	probably benign	0.05
R2087:Wdr90	UTSW	17	26,065,577 (GRCm39)	missense	probably damaging	1.00
R2159:Wdr90	UTSW	17	26,070,715 (GRCm39)	missense	probably benign
R2208:Wdr90	UTSW	17	26,079,362 (GRCm39)	missense	probably damaging	1.00
R2391:Wdr90	UTSW	17	26,070,429 (GRCm39)	missense	probably damaging	1.00
R2394:Wdr90	UTSW	17	26,070,429 (GRCm39)	missense	probably damaging	1.00
R2520:Wdr90	UTSW	17	26,074,326 (GRCm39)	missense	probably damaging	1.00
R3798:Wdr90	UTSW	17	26,069,472 (GRCm39)	missense	probably benign	0.01
R3979:Wdr90	UTSW	17	26,078,252 (GRCm39)	missense	probably benign	0.00
R4111:Wdr90	UTSW	17	26,068,342 (GRCm39)	missense	possibly damaging	0.94
R4326:Wdr90	UTSW	17	26,072,705 (GRCm39)	missense	probably benign	0.25
R4459:Wdr90	UTSW	17	26,079,976 (GRCm39)	missense	possibly damaging	0.95
R4697:Wdr90	UTSW	17	26,074,337 (GRCm39)	missense	probably benign	0.03
R4735:Wdr90	UTSW	17	26,078,424 (GRCm39)	missense	probably benign
R4907:Wdr90	UTSW	17	26,079,624 (GRCm39)	intron	probably benign
R5070:Wdr90	UTSW	17	26,065,307 (GRCm39)	missense	probably damaging	1.00
R5230:Wdr90	UTSW	17	26,074,277 (GRCm39)	missense	probably benign	0.01
R5268:Wdr90	UTSW	17	26,069,819 (GRCm39)	missense	probably damaging	1.00
R5287:Wdr90	UTSW	17	26,080,441 (GRCm39)	utr 5 prime	probably benign
R5382:Wdr90	UTSW	17	26,064,572 (GRCm39)	missense	probably damaging	1.00
R5511:Wdr90	UTSW	17	26,063,995 (GRCm39)	unclassified	probably benign
R5545:Wdr90	UTSW	17	26,064,830 (GRCm39)	missense	probably damaging	1.00
R5707:Wdr90	UTSW	17	26,076,166 (GRCm39)	missense	probably benign	0.00
R5973:Wdr90	UTSW	17	26,065,381 (GRCm39)	missense	probably damaging	1.00
R5973:Wdr90	UTSW	17	26,064,107 (GRCm39)	missense	probably damaging	0.99
R6385:Wdr90	UTSW	17	26,067,504 (GRCm39)	missense	probably damaging	1.00
R6481:Wdr90	UTSW	17	26,064,885 (GRCm39)	missense	probably damaging	0.99
R7078:Wdr90	UTSW	17	26,068,623 (GRCm39)	missense	probably damaging	1.00
R7214:Wdr90	UTSW	17	26,064,367 (GRCm39)	missense	probably benign	0.00
R7288:Wdr90	UTSW	17	26,065,286 (GRCm39)	missense	probably benign	0.03
R7304:Wdr90	UTSW	17	26,070,480 (GRCm39)	missense	probably benign	0.10
R7309:Wdr90	UTSW	17	26,079,676 (GRCm39)	missense	probably benign	0.02
R7391:Wdr90	UTSW	17	26,065,502 (GRCm39)	missense	probably benign	0.08
R7622:Wdr90	UTSW	17	26,073,083 (GRCm39)	missense	probably benign	0.00
R7646:Wdr90	UTSW	17	26,079,104 (GRCm39)	missense	probably benign	0.38
R7772:Wdr90	UTSW	17	26,080,465 (GRCm39)	start gained	probably benign
R7779:Wdr90	UTSW	17	26,065,300 (GRCm39)	missense	probably damaging	1.00
R7780:Wdr90	UTSW	17	26,065,300 (GRCm39)	missense	probably damaging	1.00
R7781:Wdr90	UTSW	17	26,065,300 (GRCm39)	missense	probably damaging	1.00
R7782:Wdr90	UTSW	17	26,065,300 (GRCm39)	missense	probably damaging	1.00
R7812:Wdr90	UTSW	17	26,071,532 (GRCm39)	missense	probably damaging	1.00
R7870:Wdr90	UTSW	17	26,079,513 (GRCm39)	missense	probably damaging	0.96
R7911:Wdr90	UTSW	17	26,069,723 (GRCm39)	missense	probably benign	0.00
R8126:Wdr90	UTSW	17	26,067,951 (GRCm39)	missense	possibly damaging	0.48
R8260:Wdr90	UTSW	17	26,064,141 (GRCm39)	missense	probably damaging	1.00
R8315:Wdr90	UTSW	17	26,064,399 (GRCm39)	missense	probably benign	0.21
R8919:Wdr90	UTSW	17	26,076,146 (GRCm39)	missense
R8938:Wdr90	UTSW	17	26,076,146 (GRCm39)	missense
R8940:Wdr90	UTSW	17	26,076,146 (GRCm39)	missense
R9038:Wdr90	UTSW	17	26,076,200 (GRCm39)	missense
R9079:Wdr90	UTSW	17	26,076,403 (GRCm39)	missense
R9401:Wdr90	UTSW	17	26,064,750 (GRCm39)	small insertion	probably benign
R9471:Wdr90	UTSW	17	26,080,015 (GRCm39)	missense	possibly damaging	0.72
R9642:Wdr90	UTSW	17	26,072,694 (GRCm39)	critical splice donor site	probably null
X0064:Wdr90	UTSW	17	26,067,537 (GRCm39)	missense	probably damaging	1.00
Z1176:Wdr90	UTSW	17	26,079,470 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CTGGCTGCAGTGGTAAAGTC -3'
(R):5'- TCCATTACAGCCATGCCAGG -3'

Sequencing Primer
(F):5'- GGTAAAGTCTTTAGAAACCCCAGGTC -3'
(R):5'- AGCCATGCCAGGTCCTTTCAG -3'

Posted On

2014-10-30