Mutagenetix > Incidental Mutation

Incidental Mutation 'R3522:Ptgfrn'

267522

Institutional Source

Beutler Lab

Gene Symbol

Ptgfrn

Ensembl Gene

ENSMUSG00000027864

Gene Name

prostaglandin F2 receptor negative regulator

Synonyms

4833445A08Rik, CD9P-1

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3522 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

100947548-101017594 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 100950718 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 865 (E865G)

Ref Sequence

ENSEMBL: ENSMUSP00000099755 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102694]

AlphaFold

Q9WV91

Predicted Effect

probably damaging
Transcript: ENSMUST00000102694
AA Change: E865G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000099755
Gene: ENSMUSG00000027864
AA Change: E865G

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
IGv	38	121	3.01e-7	SMART
IG	154	264	1.54e-4	SMART
IG	284	390	1.11e-5	SMART
IG	414	532	1.72e-2	SMART
IG	556	676	9.71e-2	SMART
IG	696	822	5.21e-2	SMART
transmembrane domain	831	853	N/A	INTRINSIC
low complexity region	862	872	N/A	INTRINSIC

Meta Mutation Damage Score

0.0999

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 94.8%

Validation Efficiency

100% (72/72)

MGI Phenotype

PHENOTYPE: Homozygous mice for a null gene trap mutation exhibit a decreased depressive-like response during tail suspension testing when compared with their wild-type littermates, [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd35	A	G	3: 96,592,378 (GRCm39)	E888G	probably damaging	Het
Arhgef10	T	C	8: 15,004,918 (GRCm39)	F150S	probably damaging	Het
Atp10d	G	T	5: 72,396,500 (GRCm39)	R235L	probably benign	Het
Cacna1b	A	G	2: 24,653,055 (GRCm39)	V2A	possibly damaging	Het
Cand1	A	T	10: 119,075,102 (GRCm39)	L15Q	probably benign	Het
Cavin3	C	A	7: 105,130,350 (GRCm39)	G154V	probably benign	Het
Ccdc73	A	T	2: 104,821,830 (GRCm39)	D593V	probably damaging	Het
Cdk5rap2	T	C	4: 70,168,647 (GRCm39)	K161E	probably damaging	Het
Chil4	A	T	3: 106,111,056 (GRCm39)	N279K	probably benign	Het
Chst13	T	G	6: 90,295,245 (GRCm39)	D56A	probably damaging	Het
Cnn1	C	A	9: 22,010,664 (GRCm39)	H5N	probably benign	Het
Cpsf4l	T	A	11: 113,593,319 (GRCm39)	K88N	probably damaging	Het
Ctnnbl1	G	T	2: 157,713,113 (GRCm39)		probably null	Het
Dnah7a	A	C	1: 53,657,275 (GRCm39)	F834V	probably damaging	Het
Fbxo41	A	G	6: 85,461,163 (GRCm39)	S182P	probably benign	Het
Fkbp5	T	C	17: 28,634,970 (GRCm39)	T180A	probably benign	Het
Flg2	T	A	3: 93,127,334 (GRCm39)	I2082N	unknown	Het
Gm4968	A	G	6: 127,210,725 (GRCm39)		noncoding transcript	Het
Gpc5	T	A	14: 116,761,747 (GRCm39)	H612Q	probably benign	Het
Gsg1	A	T	6: 135,218,251 (GRCm39)	V212D	probably damaging	Het
Hipk1	A	G	3: 103,651,430 (GRCm39)	V1111A	probably damaging	Het
Hormad1	A	T	3: 95,483,596 (GRCm39)	Q136L	probably benign	Het
Ifi35	T	A	11: 101,348,511 (GRCm39)	S147R	probably benign	Het
Iqca1l	A	G	5: 24,754,624 (GRCm39)		probably null	Het
Iqgap3	C	T	3: 87,998,089 (GRCm39)	A282V	probably null	Het
Jmy	T	C	13: 93,590,558 (GRCm39)	D515G	probably damaging	Het
Kctd10	G	A	5: 114,512,984 (GRCm39)	R64C	probably damaging	Het
Kidins220	T	C	12: 25,040,757 (GRCm39)	V121A	probably damaging	Het
Lcn3	G	A	2: 25,656,133 (GRCm39)	V63M	possibly damaging	Het
Lmx1b	T	A	2: 33,529,543 (GRCm39)	Y72F	probably benign	Het
Lrp1	T	C	10: 127,389,424 (GRCm39)	D3164G	probably damaging	Het
Mdh1b	C	T	1: 63,758,927 (GRCm39)	V222M	probably damaging	Het
Mst1	T	C	9: 107,958,702 (GRCm39)		probably benign	Het
Myo7b	C	A	18: 32,143,132 (GRCm39)	V189F	probably damaging	Het
Ndc1	T	C	4: 107,250,355 (GRCm39)	S533P	probably damaging	Het
Ndrg3	T	C	2: 156,785,947 (GRCm39)	D164G	probably damaging	Het
Nol11	C	T	11: 107,064,454 (GRCm39)	C500Y	possibly damaging	Het
Nsd3	A	G	8: 26,196,642 (GRCm39)	N1208D	probably benign	Het
Nup155	C	T	15: 8,186,162 (GRCm39)		probably benign	Het
Or5ak20	A	T	2: 85,183,347 (GRCm39)	C308S	probably benign	Het
Or6c38	A	G	10: 128,929,711 (GRCm39)	I44T	possibly damaging	Het
Or8b47	A	G	9: 38,435,081 (GRCm39)	T18A	probably damaging	Het
Or8b54	A	T	9: 38,687,016 (GRCm39)	D155V	possibly damaging	Het
Phf3	A	G	1: 30,844,684 (GRCm39)	L1425P	probably damaging	Het
Pla2r1	A	G	2: 60,279,250 (GRCm39)	Y777H	probably damaging	Het
Pld1	A	G	3: 28,085,396 (GRCm39)	E184G	probably damaging	Het
Plxna1	T	C	6: 89,314,335 (GRCm39)		probably null	Het
Ptpn13	G	T	5: 103,737,720 (GRCm39)		probably benign	Het
Pygb	G	T	2: 150,670,473 (GRCm39)	V763F	probably benign	Het
Ros1	A	C	10: 51,967,091 (GRCm39)	Y1705*	probably null	Het
Sec61a2	A	G	2: 5,898,027 (GRCm39)	F5L	probably benign	Het
Skint5	A	G	4: 113,614,102 (GRCm39)		probably null	Het
Sntg2	A	G	12: 30,362,566 (GRCm39)	V60A	probably damaging	Het
Sppl2a	A	G	2: 126,762,242 (GRCm39)	C280R	possibly damaging	Het
Srrm4	A	C	5: 116,584,603 (GRCm39)	M1R	probably null	Het
Sult1c2	T	C	17: 54,279,043 (GRCm39)	E91G	probably damaging	Het
Themis2	C	G	4: 132,512,906 (GRCm39)	R440P	probably damaging	Het
Tmem229a	A	G	6: 24,955,058 (GRCm39)	L232P	probably benign	Het
Trappc1	T	C	11: 69,215,248 (GRCm39)	F43L	probably damaging	Het
Trappc11	A	T	8: 47,951,708 (GRCm39)	Y982N	possibly damaging	Het
Trpv6	A	T	6: 41,604,339 (GRCm39)	M139K	probably damaging	Het
Txnrd3	A	G	6: 89,640,057 (GRCm39)		probably null	Het
Vmn1r184	T	A	7: 25,967,008 (GRCm39)	Y251*	probably null	Het
Vmn1r216	A	G	13: 23,283,544 (GRCm39)	N76D	possibly damaging	Het
Vmn1r71	C	A	7: 10,481,792 (GRCm39)	V233F	probably benign	Het
Vps13a	A	C	19: 16,743,857 (GRCm39)		probably benign	Het
Vwa5b2	A	G	16: 20,420,358 (GRCm39)	S756G	probably damaging	Het
Wdr36	T	A	18: 32,994,538 (GRCm39)		probably null	Het
Wdr86	A	G	5: 24,923,305 (GRCm39)	V129A	probably benign	Het
Zfyve9	A	G	4: 108,576,940 (GRCm39)	L47S	probably benign	Het

Other mutations in Ptgfrn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00949:Ptgfrn	APN	3	100,980,161 (GRCm39)	missense	probably benign	0.01
IGL01710:Ptgfrn	APN	3	100,980,404 (GRCm39)	missense	probably damaging	0.98
IGL02557:Ptgfrn	APN	3	100,967,952 (GRCm39)	critical splice donor site	probably null
IGL02740:Ptgfrn	APN	3	100,980,253 (GRCm39)	missense	possibly damaging	0.84
IGL02817:Ptgfrn	APN	3	100,968,068 (GRCm39)	missense	probably benign
IGL02948:Ptgfrn	APN	3	100,980,135 (GRCm39)	missense	probably benign	0.21
R1540:Ptgfrn	UTSW	3	100,967,970 (GRCm39)	missense	probably benign	0.41
R1563:Ptgfrn	UTSW	3	100,967,967 (GRCm39)	missense	possibly damaging	0.67
R1730:Ptgfrn	UTSW	3	100,963,758 (GRCm39)	missense	possibly damaging	0.71
R1766:Ptgfrn	UTSW	3	100,957,438 (GRCm39)	missense	probably benign	0.00
R1783:Ptgfrn	UTSW	3	100,963,758 (GRCm39)	missense	possibly damaging	0.71
R1918:Ptgfrn	UTSW	3	100,963,623 (GRCm39)	missense	probably benign
R2113:Ptgfrn	UTSW	3	100,984,625 (GRCm39)	missense	probably benign	0.00
R2290:Ptgfrn	UTSW	3	100,984,677 (GRCm39)	missense	possibly damaging	0.77
R5223:Ptgfrn	UTSW	3	100,952,909 (GRCm39)	missense	probably benign	0.13
R5600:Ptgfrn	UTSW	3	100,963,566 (GRCm39)	missense	probably damaging	0.99
R5642:Ptgfrn	UTSW	3	100,950,678 (GRCm39)	missense	probably damaging	1.00
R5927:Ptgfrn	UTSW	3	100,967,968 (GRCm39)	missense	possibly damaging	0.92
R5984:Ptgfrn	UTSW	3	100,957,459 (GRCm39)	missense	probably damaging	0.99
R6124:Ptgfrn	UTSW	3	100,980,405 (GRCm39)	missense	probably damaging	0.98
R6331:Ptgfrn	UTSW	3	100,952,936 (GRCm39)	missense	possibly damaging	0.64
R6363:Ptgfrn	UTSW	3	100,952,894 (GRCm39)	missense	possibly damaging	0.93
R6473:Ptgfrn	UTSW	3	100,952,955 (GRCm39)	missense	probably damaging	1.00
R6856:Ptgfrn	UTSW	3	100,952,762 (GRCm39)	missense	probably damaging	1.00
R7151:Ptgfrn	UTSW	3	100,987,511 (GRCm39)	nonsense	probably null
R7313:Ptgfrn	UTSW	3	100,980,363 (GRCm39)	missense	possibly damaging	0.84
R7361:Ptgfrn	UTSW	3	100,984,760 (GRCm39)	missense	probably benign	0.03
R7806:Ptgfrn	UTSW	3	100,984,448 (GRCm39)	missense	possibly damaging	0.50
R7823:Ptgfrn	UTSW	3	100,950,725 (GRCm39)	missense	probably damaging	1.00
R7841:Ptgfrn	UTSW	3	100,968,126 (GRCm39)	missense	probably damaging	0.98
R8093:Ptgfrn	UTSW	3	100,980,257 (GRCm39)	missense	probably benign	0.09
R8093:Ptgfrn	UTSW	3	100,963,753 (GRCm39)	missense	probably benign	0.19
R8490:Ptgfrn	UTSW	3	100,963,686 (GRCm39)	missense	probably damaging	0.99
R8856:Ptgfrn	UTSW	3	100,963,927 (GRCm39)	missense	possibly damaging	0.86
Z1088:Ptgfrn	UTSW	3	100,963,753 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCTCGCTCAGAACTTGACATG -3'
(R):5'- ACTAAGGAGTGAGCCAGTGC -3'

Sequencing Primer
(F):5'- CTCAGAACTTGACATGCAGAAAAG -3'
(R):5'- AGTGCTCTCTACCCTCCAG -3'

Posted On

2015-02-18