Mutagenetix > Incidental Mutation

Incidental Mutation 'R5702:Vmn2r109'

451768

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r109

Ensembl Gene

ENSMUSG00000090572

Gene Name

vomeronasal 2, receptor 109

Synonyms

EG627814

MMRRC Submission

043182-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R5702 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20760779-20785018 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 20774407 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 316 (D316G)

Ref Sequence

ENSEMBL: ENSMUSP00000132641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167093]

AlphaFold

K7N747

Predicted Effect

probably benign
Transcript: ENSMUST00000167093
AA Change: D316G

PolyPhen 2 Score 0.424 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000132641
Gene: ENSMUSG00000090572
AA Change: D316G

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	467	1.4e-35	PFAM
Pfam:NCD3G	510	563	3.1e-21	PFAM
Pfam:7tm_3	596	831	7.4e-52	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1a	G	A	5: 8,787,752 (GRCm39)	S1018N	probably benign	Het
Adat1	G	T	8: 112,704,704 (GRCm39)	T414K	probably benign	Het
Ahnak	G	T	19: 8,979,204 (GRCm39)	V163L	probably damaging	Het
Atf6b	T	C	17: 34,869,978 (GRCm39)	I288T	possibly damaging	Het
Blm	A	T	7: 80,108,675 (GRCm39)	V1323E	probably benign	Het
Brap	T	A	5: 121,803,206 (GRCm39)	L118Q	probably damaging	Het
C1qbp	G	A	11: 70,869,570 (GRCm39)	T171I	probably benign	Het
Ccdc136	C	A	6: 29,412,981 (GRCm39)	H455Q	probably damaging	Het
Chd3	A	T	11: 69,252,261 (GRCm39)	V47D	possibly damaging	Het
Cnot10	A	C	9: 114,458,078 (GRCm39)	F143V	probably damaging	Het
Cnot8	T	C	11: 58,004,873 (GRCm39)	S191P	possibly damaging	Het
Coro2b	G	A	9: 62,333,859 (GRCm39)	T345I	probably damaging	Het
Dennd1b	T	C	1: 139,061,413 (GRCm39)	I365T	probably damaging	Het
Dnah11	C	A	12: 118,077,642 (GRCm39)	A1284S	probably benign	Het
Dock4	G	A	12: 40,787,490 (GRCm39)	D802N	probably benign	Het
Dsg1a	T	C	18: 20,469,922 (GRCm39)		probably null	Het
Elp3	C	T	14: 65,815,431 (GRCm39)	R187Q	probably damaging	Het
Ercc3	G	A	18: 32,387,206 (GRCm39)	R473Q	probably damaging	Het
F5	G	A	1: 164,022,116 (GRCm39)	W1530*	probably null	Het
Fmnl1	T	C	11: 103,076,491 (GRCm39)	I219T	probably damaging	Het
Gemin4	G	A	11: 76,101,663 (GRCm39)	R1033C	probably benign	Het
Gm2381	T	A	7: 42,471,820 (GRCm39)	I20F	probably benign	Het
Hmgcll1	A	G	9: 75,991,672 (GRCm39)	M129V	possibly damaging	Het
Ltbp3	G	T	19: 5,797,849 (GRCm39)	R496L	probably benign	Het
Mettl13	A	G	1: 162,373,549 (GRCm39)	V234A	probably benign	Het
Minar1	G	A	9: 89,473,208 (GRCm39)	A901V	probably benign	Het
Or1j4	A	G	2: 36,740,946 (GRCm39)	D296G	probably damaging	Het
Pde7a	A	T	3: 19,295,371 (GRCm39)	C146*	probably null	Het
Pla2g4e	T	A	2: 120,018,992 (GRCm39)	N202Y	possibly damaging	Het
Plk5	G	A	10: 80,196,401 (GRCm39)		probably null	Het
Plscr1l1	G	A	9: 92,225,741 (GRCm39)		probably null	Het
Ptpn7	A	G	1: 135,061,582 (GRCm39)	T15A	probably benign	Het
Rrn3	T	A	16: 13,631,130 (GRCm39)	Y655*	probably null	Het
Scarb2	C	T	5: 92,599,255 (GRCm39)	G355D	probably damaging	Het
Scd2	G	A	19: 44,286,502 (GRCm39)	A111T	possibly damaging	Het
Smim8	TTTAATGAAGAGCT	TT	4: 34,771,261 (GRCm39)		probably benign	Het
Smurf1	T	C	5: 144,838,021 (GRCm39)	T57A	possibly damaging	Het
Spire2	C	T	8: 124,073,402 (GRCm39)	P94S	probably benign	Het
Tex2	T	C	11: 106,435,221 (GRCm39)	H237R	possibly damaging	Het
Zfp830	T	A	11: 82,655,800 (GRCm39)	F201L	possibly damaging	Het
Zic1	A	G	9: 91,246,133 (GRCm39)	F313S	probably damaging	Het

Other mutations in Vmn2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01313:Vmn2r109	APN	17	20,770,419 (GRCm39)	missense	probably damaging	1.00
IGL01383:Vmn2r109	APN	17	20,761,383 (GRCm39)	missense	possibly damaging	0.89
IGL01469:Vmn2r109	APN	17	20,761,671 (GRCm39)	missense	probably damaging	1.00
IGL01762:Vmn2r109	APN	17	20,774,654 (GRCm39)	missense	probably benign
IGL01864:Vmn2r109	APN	17	20,761,396 (GRCm39)	missense	probably benign	0.28
IGL02028:Vmn2r109	APN	17	20,761,342 (GRCm39)	missense	probably benign	0.28
IGL02074:Vmn2r109	APN	17	20,774,603 (GRCm39)	missense	probably benign	0.05
IGL02162:Vmn2r109	APN	17	20,774,422 (GRCm39)	missense	probably benign	0.01
IGL02474:Vmn2r109	APN	17	20,761,150 (GRCm39)	missense	probably benign
IGL02490:Vmn2r109	APN	17	20,761,246 (GRCm39)	missense	possibly damaging	0.78
IGL02604:Vmn2r109	APN	17	20,760,963 (GRCm39)	missense	probably damaging	1.00
IGL02669:Vmn2r109	APN	17	20,774,518 (GRCm39)	missense	possibly damaging	0.64
IGL02705:Vmn2r109	APN	17	20,774,062 (GRCm39)	missense	probably benign
IGL02745:Vmn2r109	APN	17	20,761,512 (GRCm39)	missense	probably damaging	0.99
PIT4142001:Vmn2r109	UTSW	17	20,774,839 (GRCm39)	critical splice acceptor site	probably null
R0389:Vmn2r109	UTSW	17	20,761,336 (GRCm39)	missense	probably damaging	1.00
R0470:Vmn2r109	UTSW	17	20,773,148 (GRCm39)	missense	probably benign	0.06
R0570:Vmn2r109	UTSW	17	20,760,937 (GRCm39)	missense	probably damaging	0.99
R0855:Vmn2r109	UTSW	17	20,761,670 (GRCm39)	nonsense	probably null
R0882:Vmn2r109	UTSW	17	20,774,842 (GRCm39)	splice site	probably benign
R1241:Vmn2r109	UTSW	17	20,775,503 (GRCm39)	missense	possibly damaging	0.86
R1587:Vmn2r109	UTSW	17	20,761,002 (GRCm39)	missense	probably damaging	1.00
R1931:Vmn2r109	UTSW	17	20,774,072 (GRCm39)	nonsense	probably null
R1957:Vmn2r109	UTSW	17	20,784,969 (GRCm39)	missense	probably benign	0.11
R1962:Vmn2r109	UTSW	17	20,774,185 (GRCm39)	missense	probably damaging	0.99
R2020:Vmn2r109	UTSW	17	20,761,448 (GRCm39)	nonsense	probably null
R2073:Vmn2r109	UTSW	17	20,784,974 (GRCm39)	missense	probably benign	0.00
R2436:Vmn2r109	UTSW	17	20,774,798 (GRCm39)	missense	probably damaging	0.99
R3123:Vmn2r109	UTSW	17	20,761,248 (GRCm39)	missense	probably damaging	1.00
R3839:Vmn2r109	UTSW	17	20,774,704 (GRCm39)	missense	probably damaging	1.00
R4019:Vmn2r109	UTSW	17	20,774,074 (GRCm39)	missense	probably benign
R4428:Vmn2r109	UTSW	17	20,773,286 (GRCm39)	missense	probably benign
R4584:Vmn2r109	UTSW	17	20,774,820 (GRCm39)	nonsense	probably null
R4652:Vmn2r109	UTSW	17	20,761,656 (GRCm39)	missense	probably damaging	1.00
R4708:Vmn2r109	UTSW	17	20,761,605 (GRCm39)	missense	probably damaging	0.97
R4823:Vmn2r109	UTSW	17	20,774,153 (GRCm39)	missense	probably damaging	1.00
R4831:Vmn2r109	UTSW	17	20,761,494 (GRCm39)	missense	probably benign	0.01
R4907:Vmn2r109	UTSW	17	20,770,348 (GRCm39)	missense	probably damaging	1.00
R5011:Vmn2r109	UTSW	17	20,775,451 (GRCm39)	missense	probably damaging	1.00
R5296:Vmn2r109	UTSW	17	20,774,603 (GRCm39)	missense	possibly damaging	0.90
R5600:Vmn2r109	UTSW	17	20,761,189 (GRCm39)	missense	probably damaging	1.00
R5602:Vmn2r109	UTSW	17	20,760,933 (GRCm39)	missense	possibly damaging	0.94
R5652:Vmn2r109	UTSW	17	20,760,781 (GRCm39)	makesense	probably null
R5706:Vmn2r109	UTSW	17	20,774,567 (GRCm39)	missense	probably benign	0.16
R5714:Vmn2r109	UTSW	17	20,773,121 (GRCm39)	missense	probably damaging	1.00
R5832:Vmn2r109	UTSW	17	20,761,318 (GRCm39)	missense	probably benign	0.10
R6008:Vmn2r109	UTSW	17	20,760,981 (GRCm39)	missense	probably damaging	1.00
R6334:Vmn2r109	UTSW	17	20,761,440 (GRCm39)	missense	probably benign	0.18
R6377:Vmn2r109	UTSW	17	20,784,796 (GRCm39)	critical splice donor site	probably null
R6738:Vmn2r109	UTSW	17	20,774,785 (GRCm39)	missense	possibly damaging	0.52
R6857:Vmn2r109	UTSW	17	20,760,932 (GRCm39)	missense	probably benign	0.45
R6953:Vmn2r109	UTSW	17	20,760,973 (GRCm39)	missense	possibly damaging	0.95
R7108:Vmn2r109	UTSW	17	20,785,006 (GRCm39)	missense	probably benign	0.03
R7229:Vmn2r109	UTSW	17	20,761,225 (GRCm39)	missense	possibly damaging	0.80
R7238:Vmn2r109	UTSW	17	20,761,336 (GRCm39)	missense	probably damaging	1.00
R7244:Vmn2r109	UTSW	17	20,760,945 (GRCm39)	missense	possibly damaging	0.70
R7292:Vmn2r109	UTSW	17	20,761,700 (GRCm39)	missense	probably benign	0.05
R7354:Vmn2r109	UTSW	17	20,761,043 (GRCm39)	missense	probably damaging	1.00
R7357:Vmn2r109	UTSW	17	20,761,536 (GRCm39)	missense	probably damaging	1.00
R7522:Vmn2r109	UTSW	17	20,774,665 (GRCm39)	missense	probably benign	0.11
R7596:Vmn2r109	UTSW	17	20,760,942 (GRCm39)	missense	probably damaging	0.98
R7728:Vmn2r109	UTSW	17	20,773,117 (GRCm39)	missense	probably damaging	0.99
R7859:Vmn2r109	UTSW	17	20,761,436 (GRCm39)	missense	probably damaging	1.00
R7871:Vmn2r109	UTSW	17	20,760,782 (GRCm39)	missense	probably benign	0.08
R8113:Vmn2r109	UTSW	17	20,774,729 (GRCm39)	missense	probably benign	0.01
R8153:Vmn2r109	UTSW	17	20,784,969 (GRCm39)	missense	probably benign	0.11
R8977:Vmn2r109	UTSW	17	20,774,531 (GRCm39)	missense	possibly damaging	0.96
R9687:Vmn2r109	UTSW	17	20,775,332 (GRCm39)	missense
Z1176:Vmn2r109	UTSW	17	20,773,256 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCATTGGAGTGACAGTCAGC -3'
(R):5'- CTTTTGTGAAAATGATCCCTGACACG -3'

Sequencing Primer
(F):5'- TGACAGTCAGCCAACAGTTG -3'
(R):5'- AATGATCCCTGACACGTGGGC -3'

Posted On

2017-01-03